Glutathione synthetase

Quick info Annotations Localization Phosphorylation Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

glutathione synthetase - putative

Species:

Leishmania infantum

UniProt:

A4HW34_LEIIN

TriTrypDb:

LINF_140015200

Length:

606

Annotations

Annotations by Jardim et al.

Redox homeostasis, Glutathione synthetase

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	1
GO:0005737	cytoplasm	2	1
GO:0005829	cytosol	2	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	2
GO:0016020	membrane	2	1

Phosphorylation

Promastigote: 223, 228

Expansion

Sequence features

A4HW34

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HW34

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0000287	magnesium ion binding	5	12
GO:0003824	catalytic activity	1	12
GO:0004363	glutathione synthase activity	3	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	12
GO:0016881	acid-amino acid ligase activity	4	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0033218	amide binding	2	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0042277	peptide binding	3	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0043169	cation binding	3	12
GO:0043295	glutathione binding	3	12
GO:0046872	metal ion binding	4	12
GO:0072341	modified amino acid binding	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1900750	oligopeptide binding	4	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12
GO:1901681	sulfur compound binding	2	12
GO:1904091	non-ribosomal peptide synthetase activity	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	586	590	PF00656	0.446
CLV_NRD_NRD_1	315	317	PF00675	0.318
CLV_NRD_NRD_1	521	523	PF00675	0.353
CLV_NRD_NRD_1	527	529	PF00675	0.337
CLV_PCSK_KEX2_1	315	317	PF00082	0.318
CLV_PCSK_KEX2_1	521	523	PF00082	0.337
CLV_PCSK_SKI1_1	102	106	PF00082	0.422
CLV_PCSK_SKI1_1	199	203	PF00082	0.465
CLV_PCSK_SKI1_1	300	304	PF00082	0.400
CLV_PCSK_SKI1_1	454	458	PF00082	0.313
CLV_PCSK_SKI1_1	484	488	PF00082	0.270
CLV_PCSK_SKI1_1	508	512	PF00082	0.355
CLV_PCSK_SKI1_1	72	76	PF00082	0.341
DEG_APCC_DBOX_1	314	322	PF00400	0.315
DEG_SPOP_SBC_1	10	14	PF00917	0.445
DEG_SPOP_SBC_1	2	6	PF00917	0.551
DOC_CKS1_1	117	122	PF01111	0.422
DOC_CYCLIN_RxL_1	297	307	PF00134	0.449
DOC_CYCLIN_yCln2_LP_2	351	357	PF00134	0.449
DOC_MAPK_DCC_7	275	285	PF00069	0.449
DOC_MAPK_gen_1	197	205	PF00069	0.371
DOC_MAPK_gen_1	360	366	PF00069	0.235
DOC_MAPK_JIP1_4	281	287	PF00069	0.422
DOC_MAPK_MEF2A_6	33	41	PF00069	0.397
DOC_MAPK_RevD_3	301	316	PF00069	0.362
DOC_PP1_RVXF_1	70	77	PF00149	0.342
DOC_PP1_SILK_1	133	138	PF00149	0.371
DOC_PP2B_LxvP_1	351	354	PF13499	0.449
DOC_USP7_MATH_1	10	14	PF00917	0.646
DOC_USP7_MATH_1	220	224	PF00917	0.387
DOC_USP7_MATH_1	264	268	PF00917	0.441
DOC_USP7_MATH_1	575	579	PF00917	0.352
DOC_USP7_MATH_1	598	602	PF00917	0.302
DOC_WW_Pin1_4	116	121	PF00397	0.389
DOC_WW_Pin1_4	182	187	PF00397	0.326
DOC_WW_Pin1_4	225	230	PF00397	0.420
LIG_14-3-3_CanoR_1	315	319	PF00244	0.317
LIG_14-3-3_CanoR_1	370	374	PF00244	0.318
LIG_14-3-3_CanoR_1	550	559	PF00244	0.364
LIG_Actin_WH2_2	299	317	PF00022	0.369
LIG_BIR_III_2	261	265	PF00653	0.235
LIG_BIR_III_4	566	570	PF00653	0.373
LIG_BRCT_BRCA1_1	177	181	PF00533	0.302
LIG_BRCT_BRCA1_1	83	87	PF00533	0.371
LIG_FHA_1	103	109	PF00498	0.302
LIG_FHA_1	116	122	PF00498	0.302
LIG_FHA_1	306	312	PF00498	0.407
LIG_FHA_1	322	328	PF00498	0.280
LIG_FHA_1	44	50	PF00498	0.404
LIG_FHA_2	341	347	PF00498	0.323
LIG_FHA_2	509	515	PF00498	0.406
LIG_LIR_Apic_2	218	222	PF02991	0.274
LIG_LIR_Gen_1	200	210	PF02991	0.371
LIG_LIR_Gen_1	400	411	PF02991	0.318
LIG_LIR_Gen_1	449	456	PF02991	0.367
LIG_LIR_Gen_1	523	532	PF02991	0.259
LIG_LIR_Gen_1	84	94	PF02991	0.388
LIG_LIR_Nem_3	178	184	PF02991	0.337
LIG_LIR_Nem_3	200	205	PF02991	0.371
LIG_LIR_Nem_3	297	302	PF02991	0.337
LIG_LIR_Nem_3	449	455	PF02991	0.367
LIG_LIR_Nem_3	514	520	PF02991	0.422
LIG_LIR_Nem_3	64	70	PF02991	0.241
LIG_LIR_Nem_3	84	90	PF02991	0.371
LIG_NRBOX	301	307	PF00104	0.449
LIG_Pex14_2	198	202	PF04695	0.371
LIG_Rb_pABgroove_1	64	72	PF01858	0.410
LIG_REV1ctd_RIR_1	537	546	PF16727	0.371
LIG_SH2_CRK	128	132	PF00017	0.321
LIG_SH2_CRK	219	223	PF00017	0.235
LIG_SH2_CRK	299	303	PF00017	0.387
LIG_SH2_CRK	452	456	PF00017	0.371
LIG_SH2_CRK	517	521	PF00017	0.318
LIG_SH2_NCK_1	219	223	PF00017	0.235
LIG_SH2_STAP1	265	269	PF00017	0.371
LIG_SH2_STAP1	452	456	PF00017	0.371
LIG_SH2_STAP1	70	74	PF00017	0.410
LIG_SH2_STAT3	70	73	PF00017	0.422
LIG_SH2_STAT5	19	22	PF00017	0.390
LIG_SH2_STAT5	194	197	PF00017	0.364
LIG_SH2_STAT5	236	239	PF00017	0.410
LIG_SH2_STAT5	362	365	PF00017	0.315
LIG_SH2_STAT5	368	371	PF00017	0.262
LIG_SH2_STAT5	373	376	PF00017	0.249
LIG_SH2_STAT5	485	488	PF00017	0.322
LIG_SH2_STAT5	524	527	PF00017	0.302
LIG_SH2_STAT5	570	573	PF00017	0.339
LIG_SH3_3	124	130	PF00018	0.420
LIG_SH3_3	393	399	PF00018	0.354
LIG_SH3_3	418	424	PF00018	0.318
LIG_SH3_3	476	482	PF00018	0.324
LIG_SH3_3	483	489	PF00018	0.280
LIG_SUMO_SIM_par_1	107	113	PF11976	0.446
LIG_SUMO_SIM_par_1	301	308	PF11976	0.427
LIG_TRAF2_1	25	28	PF00917	0.478
LIG_TRFH_1	485	489	PF08558	0.302
LIG_UBA3_1	108	114	PF00899	0.369
LIG_WRC_WIRS_1	265	270	PF05994	0.371
LIG_WRC_WIRS_1	405	410	PF05994	0.318
MOD_CDK_SPK_2	225	230	PF00069	0.371
MOD_CDK_SPxxK_3	225	232	PF00069	0.332
MOD_CK1_1	150	156	PF00069	0.457
MOD_CK1_1	340	346	PF00069	0.449
MOD_CK1_1	416	422	PF00069	0.418
MOD_CK1_1	93	99	PF00069	0.429
MOD_CK2_1	107	113	PF00069	0.436
MOD_CK2_1	150	156	PF00069	0.404
MOD_CK2_1	23	29	PF00069	0.455
MOD_CK2_1	443	449	PF00069	0.438
MOD_CK2_1	93	99	PF00069	0.397
MOD_GlcNHglycan	147	150	PF01048	0.389
MOD_GlcNHglycan	153	156	PF01048	0.383
MOD_GlcNHglycan	233	236	PF01048	0.365
MOD_GlcNHglycan	243	246	PF01048	0.320
MOD_GlcNHglycan	248	251	PF01048	0.274
MOD_GSK3_1	135	142	PF00069	0.422
MOD_GSK3_1	147	154	PF00069	0.333
MOD_GSK3_1	173	180	PF00069	0.302
MOD_GSK3_1	2	9	PF00069	0.727
MOD_GSK3_1	211	218	PF00069	0.377
MOD_GSK3_1	220	227	PF00069	0.368
MOD_GSK3_1	231	238	PF00069	0.185
MOD_GSK3_1	310	317	PF00069	0.318
MOD_GSK3_1	379	386	PF00069	0.369
MOD_GSK3_1	43	50	PF00069	0.387
MOD_GSK3_1	575	582	PF00069	0.426
MOD_GSK3_1	90	97	PF00069	0.429
MOD_N-GLC_1	416	421	PF02516	0.422
MOD_N-GLC_1	80	85	PF02516	0.359
MOD_N-GLC_2	551	553	PF02516	0.310
MOD_NEK2_1	305	310	PF00069	0.357
MOD_NEK2_1	314	319	PF00069	0.310
MOD_NEK2_1	321	326	PF00069	0.204
MOD_NEK2_1	369	374	PF00069	0.307
MOD_NEK2_1	579	584	PF00069	0.342
MOD_NEK2_1	90	95	PF00069	0.354
MOD_NEK2_2	310	315	PF00069	0.338
MOD_NEK2_2	52	57	PF00069	0.422
MOD_PK_1	107	113	PF00069	0.313
MOD_PK_1	397	403	PF00069	0.318
MOD_PKA_2	231	237	PF00069	0.371
MOD_PKA_2	314	320	PF00069	0.318
MOD_PKA_2	369	375	PF00069	0.318
MOD_PKA_2	81	87	PF00069	0.371
MOD_PKA_2	90	96	PF00069	0.354
MOD_Plk_2-3	509	515	PF00069	0.449
MOD_Plk_4	107	113	PF00069	0.376
MOD_Plk_4	220	226	PF00069	0.429
MOD_Plk_4	264	270	PF00069	0.371
MOD_Plk_4	369	375	PF00069	0.302
MOD_Plk_4	575	581	PF00069	0.402
MOD_ProDKin_1	116	122	PF00069	0.389
MOD_ProDKin_1	182	188	PF00069	0.326
MOD_ProDKin_1	225	231	PF00069	0.420
MOD_SUMO_rev_2	463	468	PF00179	0.457
TRG_DiLeu_BaLyEn_6	104	109	PF01217	0.387
TRG_ENDOCYTIC_2	128	131	PF00928	0.347
TRG_ENDOCYTIC_2	265	268	PF00928	0.437
TRG_ENDOCYTIC_2	299	302	PF00928	0.387
TRG_ENDOCYTIC_2	403	406	PF00928	0.318
TRG_ENDOCYTIC_2	452	455	PF00928	0.371
TRG_ENDOCYTIC_2	517	520	PF00928	0.302
TRG_ENDOCYTIC_2	524	527	PF00928	0.302
TRG_ER_diArg_1	229	232	PF00400	0.432
TRG_ER_diArg_1	314	316	PF00400	0.305
TRG_ER_diArg_1	459	462	PF00400	0.371
TRG_ER_diArg_1	520	522	PF00400	0.337
TRG_ER_diArg_1	542	545	PF00400	0.245
TRG_NLS_MonoExtC_3	517	522	PF00514	0.271
TRG_NLS_MonoExtN_4	515	522	PF00514	0.157
TRG_Pf-PMV_PEXEL_1	300	304	PF00026	0.400
TRG_Pf-PMV_PEXEL_1	316	320	PF00026	0.199
TRG_Pf-PMV_PEXEL_1	505	509	PF00026	0.422

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IHC1	Leptomonas seymouri	63%	95%
A0A0S4J7U4	Bodo saltans	37%	100%
A0A1X0NNJ6	Trypanosomatidae	44%	100%
A0A3Q8IC15	Leishmania donovani	98%	100%
A0A3R7LQL5	Trypanosoma rangeli	46%	100%
A4H7P4	Leishmania braziliensis	76%	100%
C9ZSZ1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
E9APT6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
O22494	Solanum lycopersicum	29%	100%
O23732	Brassica juncea	29%	100%
P35668	Xenopus laevis	28%	100%
P35669	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	100%
P46413	Rattus norvegicus	28%	100%
P46416	Arabidopsis thaliana	29%	100%
P48637	Homo sapiens	28%	100%
P51855	Mus musculus	28%	100%
Q08220	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	100%
Q4QFP4	Leishmania major	91%	100%
Q54E83	Dictyostelium discoideum	26%	100%
Q5EAC2	Bos taurus	27%	100%
Q8HXX5	Macaca fascicularis	27%	100%
V5BNE3	Trypanosoma cruzi	47%	100%