Vacuolar calcium ion transporter

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein - conserved

Species:

Leishmania infantum

UniProt:

A4HVW9_LEIIN

TriTrypDb:

LINF_140007700

Length:

494

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005774	vacuolar membrane	5	7
GO:0016020	membrane	2	8
GO:0031090	organelle membrane	3	7
GO:0098588	bounding membrane of organelle	4	7
GO:0110165	cellular anatomical entity	1	8

Expansion

Sequence features

A4HVW9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HVW9

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	1
GO:0006811	monoatomic ion transport	4	1
GO:0006812	monoatomic cation transport	5	1
GO:0006816	calcium ion transport	7	1
GO:0006873	intracellular monoatomic ion homeostasis	4	1
GO:0006874	intracellular calcium ion homeostasis	7	1
GO:0006875	obsolete intracellular metal ion homeostasis	6	1
GO:0009987	cellular process	1	1
GO:0019725	cellular homeostasis	2	1
GO:0030001	metal ion transport	6	1
GO:0030003	intracellular monoatomic cation homeostasis	5	1
GO:0034220	monoatomic ion transmembrane transport	3	1
GO:0042592	homeostatic process	3	1
GO:0048878	chemical homeostasis	4	1
GO:0050801	monoatomic ion homeostasis	5	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0055065	obsolete metal ion homeostasis	7	1
GO:0055074	calcium ion homeostasis	8	1
GO:0055080	monoatomic cation homeostasis	6	1
GO:0055082	intracellular chemical homeostasis	3	1
GO:0055085	transmembrane transport	2	1
GO:0065007	biological regulation	1	1
GO:0065008	regulation of biological quality	2	1
GO:0070588	calcium ion transmembrane transport	6	1
GO:0072503	obsolete cellular divalent inorganic cation homeostasis	6	1
GO:0072507	obsolete divalent inorganic cation homeostasis	7	1
GO:0098655	monoatomic cation transmembrane transport	4	1
GO:0098660	inorganic ion transmembrane transport	4	1
GO:0098662	inorganic cation transmembrane transport	5	1
GO:0098771	inorganic ion homeostasis	6	1

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	8
GO:0008324	monoatomic cation transmembrane transporter activity	4	8
GO:0015075	monoatomic ion transmembrane transporter activity	3	8
GO:0022857	transmembrane transporter activity	2	8
GO:0005451	obsolete monoatomic cation:proton antiporter activity	5	3
GO:0015078	proton transmembrane transporter activity	5	3
GO:0015085	calcium ion transmembrane transporter activity	6	3
GO:0015291	secondary active transmembrane transporter activity	4	3
GO:0015297	antiporter activity	5	3
GO:0015298	obsolete solute:monoatomic cation antiporter activity	5	3
GO:0015299	obsolete solute:proton antiporter activity	6	3
GO:0015318	inorganic molecular entity transmembrane transporter activity	3	3
GO:0015368	calcium:monoatomic cation antiporter activity	7	3
GO:0015369	calcium:proton antiporter activity	8	3
GO:0022804	active transmembrane transporter activity	3	3
GO:0022853	active monoatomic ion transmembrane transporter activity	4	3
GO:0022890	inorganic cation transmembrane transporter activity	4	3
GO:0046873	metal ion transmembrane transporter activity	5	3
GO:0051139	metal cation:proton antiporter activity	7	3
GO:0140828	metal cation:monoatomic cation antiporter activity	6	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	35	39	PF00656	0.754
CLV_C14_Caspase3-7	92	96	PF00656	0.506
CLV_NRD_NRD_1	249	251	PF00675	0.712
CLV_PCSK_KEX2_1	170	172	PF00082	0.587
CLV_PCSK_KEX2_1	249	251	PF00082	0.712
CLV_PCSK_PC1ET2_1	170	172	PF00082	0.587
CLV_PCSK_SKI1_1	177	181	PF00082	0.497
CLV_PCSK_SKI1_1	361	365	PF00082	0.555
CLV_PCSK_SKI1_1	74	78	PF00082	0.388
DEG_Nend_Nbox_1	1	3	PF02207	0.757
DEG_SPOP_SBC_1	41	45	PF00917	0.708
DOC_CDC14_PxL_1	413	421	PF14671	0.459
DOC_CYCLIN_yCln2_LP_2	427	433	PF00134	0.342
DOC_CYCLIN_yCln2_LP_2	79	85	PF00134	0.386
DOC_MAPK_gen_1	317	326	PF00069	0.575
DOC_MAPK_MEF2A_6	139	147	PF00069	0.574
DOC_MAPK_MEF2A_6	361	370	PF00069	0.331
DOC_MAPK_MEF2A_6	74	83	PF00069	0.327
DOC_PP1_RVXF_1	169	176	PF00149	0.334
DOC_PP1_RVXF_1	456	462	PF00149	0.591
DOC_PP2B_LxvP_1	121	124	PF13499	0.410
DOC_PP2B_LxvP_1	197	200	PF13499	0.410
DOC_PP2B_LxvP_1	427	430	PF13499	0.387
DOC_PP2B_LxvP_1	79	82	PF13499	0.386
DOC_PP4_FxxP_1	229	232	PF00568	0.328
DOC_PP4_FxxP_1	414	417	PF00568	0.362
DOC_SPAK_OSR1_1	333	337	PF12202	0.459
DOC_USP7_MATH_1	385	389	PF00917	0.534
DOC_USP7_MATH_1	404	408	PF00917	0.171
DOC_USP7_MATH_1	489	493	PF00917	0.515
DOC_USP7_MATH_1	96	100	PF00917	0.523
DOC_WW_Pin1_4	139	144	PF00397	0.649
DOC_WW_Pin1_4	298	303	PF00397	0.674
DOC_WW_Pin1_4	36	41	PF00397	0.735
LIG_14-3-3_CanoR_1	17	23	PF00244	0.753
LIG_14-3-3_CanoR_1	329	337	PF00244	0.565
LIG_14-3-3_CanoR_1	33	42	PF00244	0.753
LIG_Actin_WH2_2	305	321	PF00022	0.623
LIG_BRCT_BRCA1_1	330	334	PF00533	0.546
LIG_BRCT_BRCA1_1	4	8	PF00533	0.662
LIG_CaM_IQ_9	309	324	PF13499	0.576
LIG_CSL_BTD_1	79	82	PF09270	0.434
LIG_CtBP_PxDLS_1	430	434	PF00389	0.369
LIG_FHA_1	123	129	PF00498	0.386
LIG_FHA_1	21	27	PF00498	0.753
LIG_FHA_1	319	325	PF00498	0.648
LIG_FHA_1	346	352	PF00498	0.417
LIG_FHA_1	369	375	PF00498	0.416
LIG_FHA_1	404	410	PF00498	0.479
LIG_FHA_1	445	451	PF00498	0.432
LIG_FHA_2	203	209	PF00498	0.565
LIG_IRF3_LxIS_1	207	212	PF10401	0.587
LIG_LIR_Apic_2	172	178	PF02991	0.356
LIG_LIR_Apic_2	226	232	PF02991	0.305
LIG_LIR_Gen_1	158	167	PF02991	0.422
LIG_LIR_Gen_1	212	222	PF02991	0.597
LIG_LIR_Gen_1	331	341	PF02991	0.553
LIG_LIR_LC3C_4	135	138	PF02991	0.567
LIG_LIR_LC3C_4	446	450	PF02991	0.459
LIG_LIR_Nem_3	158	164	PF02991	0.379
LIG_LIR_Nem_3	212	217	PF02991	0.559
LIG_LIR_Nem_3	432	438	PF02991	0.301
LIG_LIR_Nem_3	66	72	PF02991	0.620
LIG_NRBOX	184	190	PF00104	0.459
LIG_PCNA_yPIPBox_3	126	139	PF02747	0.386
LIG_PTB_Apo_2	262	269	PF02174	0.264
LIG_SH2_CRK	264	268	PF00017	0.330
LIG_SH2_CRK	412	416	PF00017	0.459
LIG_SH2_CRK	471	475	PF00017	0.362
LIG_SH2_STAP1	281	285	PF00017	0.302
LIG_SH2_STAP1	310	314	PF00017	0.570
LIG_SH2_STAP1	330	334	PF00017	0.546
LIG_SH2_STAP1	471	475	PF00017	0.410
LIG_SH2_STAT3	310	313	PF00017	0.534
LIG_SH2_STAT5	132	135	PF00017	0.559
LIG_SH2_STAT5	284	287	PF00017	0.354
LIG_SH2_STAT5	72	75	PF00017	0.471
LIG_SH3_3	236	242	PF00018	0.532
LIG_SH3_3	299	305	PF00018	0.665
LIG_SH3_3	363	369	PF00018	0.296
LIG_SH3_3	99	105	PF00018	0.509
LIG_SUMO_SIM_anti_2	371	377	PF11976	0.377
LIG_SUMO_SIM_par_1	119	125	PF11976	0.350
LIG_SUMO_SIM_par_1	126	131	PF11976	0.301
LIG_SUMO_SIM_par_1	350	356	PF11976	0.435
LIG_SUMO_SIM_par_1	429	434	PF11976	0.412
MOD_CK1_1	169	175	PF00069	0.338
MOD_CK1_1	20	26	PF00069	0.701
MOD_CK1_1	212	218	PF00069	0.555
MOD_CK1_1	27	33	PF00069	0.680
MOD_CK1_1	298	304	PF00069	0.709
MOD_CK1_1	328	334	PF00069	0.580
MOD_CK1_1	36	42	PF00069	0.744
MOD_GlcNHglycan	217	220	PF01048	0.363
MOD_GlcNHglycan	26	29	PF01048	0.517
MOD_GlcNHglycan	35	38	PF01048	0.519
MOD_GlcNHglycan	4	7	PF01048	0.514
MOD_GlcNHglycan	98	101	PF01048	0.723
MOD_GSK3_1	13	20	PF00069	0.724
MOD_GSK3_1	32	39	PF00069	0.760
MOD_GSK3_1	341	348	PF00069	0.453
MOD_GSK3_1	385	392	PF00069	0.561
MOD_GSK3_1	90	97	PF00069	0.628
MOD_NEK2_1	1	6	PF00069	0.741
MOD_NEK2_1	159	164	PF00069	0.328
MOD_NEK2_1	166	171	PF00069	0.337
MOD_NEK2_1	181	186	PF00069	0.236
MOD_NEK2_1	192	197	PF00069	0.353
MOD_NEK2_1	209	214	PF00069	0.534
MOD_NEK2_1	217	222	PF00069	0.463
MOD_NEK2_1	267	272	PF00069	0.328
MOD_NEK2_1	32	37	PF00069	0.727
MOD_NEK2_1	353	358	PF00069	0.432
MOD_NEK2_1	431	436	PF00069	0.370
MOD_NEK2_1	444	449	PF00069	0.324
MOD_NEK2_1	63	68	PF00069	0.591
MOD_PIKK_1	328	334	PF00454	0.611
MOD_PIKK_1	389	395	PF00454	0.564
MOD_PKA_2	318	324	PF00069	0.641
MOD_PKA_2	32	38	PF00069	0.754
MOD_PKA_2	328	334	PF00069	0.492
MOD_Plk_1	157	163	PF00069	0.428
MOD_Plk_1	431	437	PF00069	0.366
MOD_Plk_2-3	203	209	PF00069	0.581
MOD_Plk_4	124	130	PF00069	0.381
MOD_Plk_4	181	187	PF00069	0.340
MOD_Plk_4	192	198	PF00069	0.310
MOD_Plk_4	223	229	PF00069	0.348
MOD_Plk_4	230	236	PF00069	0.293
MOD_Plk_4	27	33	PF00069	0.732
MOD_Plk_4	304	310	PF00069	0.580
MOD_Plk_4	336	342	PF00069	0.388
MOD_Plk_4	385	391	PF00069	0.525
MOD_Plk_4	404	410	PF00069	0.410
MOD_Plk_4	444	450	PF00069	0.410
MOD_Plk_4	463	469	PF00069	0.527
MOD_ProDKin_1	139	145	PF00069	0.649
MOD_ProDKin_1	298	304	PF00069	0.665
MOD_ProDKin_1	36	42	PF00069	0.736
TRG_DiLeu_BaEn_1	463	468	PF01217	0.497
TRG_DiLeu_BaLyEn_6	99	104	PF01217	0.407
TRG_ENDOCYTIC_2	264	267	PF00928	0.423
TRG_ENDOCYTIC_2	412	415	PF00928	0.410
TRG_ENDOCYTIC_2	471	474	PF00928	0.328
TRG_ER_diArg_1	248	250	PF00400	0.500
TRG_ER_diArg_1	458	461	PF00400	0.582

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HT55	Leptomonas seymouri	63%	100%
A0A0S4J0Y4	Bodo saltans	28%	100%
A0A1X0NNQ4	Trypanosomatidae	32%	100%
A0A3Q8ID24	Leishmania donovani	99%	100%
E9APM0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
O59768	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	100%
P74072	Synechocystis sp. (strain PCC 6803 / Kazusa)	25%	100%
Q39253	Arabidopsis thaliana	25%	100%
Q39254	Arabidopsis thaliana	24%	100%
Q4QFV8	Leishmania major	93%	100%
Q5KQN0	Oryza sativa subsp. japonica	24%	100%
Q6K1C4	Oryza sativa subsp. japonica	25%	100%
Q75XW3	Aphanothece halophytica	27%	100%
Q8L783	Arabidopsis thaliana	24%	100%
Q945S5	Arabidopsis thaliana	22%	100%