tRNA pseudouridine(55) synthase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein - conserved

Species:

Leishmania infantum

UniProt:

A4HUW4_LEIIN

TriTrypDb:

LINF_110011000

Length:

720

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A4HUW4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HUW4

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006399	tRNA metabolic process	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008033	tRNA processing	8	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0034470	ncRNA processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006400	tRNA modification	6	1
GO:0031119	tRNA pseudouridine synthesis	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0009982	pseudouridine synthase activity	4	12
GO:0016853	isomerase activity	2	12
GO:0016866	intramolecular transferase activity	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0106029	tRNA pseudouridine synthase activity	5	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	94	98	PF00656	0.748
CLV_NRD_NRD_1	250	252	PF00675	0.467
CLV_NRD_NRD_1	426	428	PF00675	0.410
CLV_NRD_NRD_1	562	564	PF00675	0.539
CLV_NRD_NRD_1	582	584	PF00675	0.248
CLV_NRD_NRD_1	644	646	PF00675	0.467
CLV_NRD_NRD_1	649	651	PF00675	0.389
CLV_NRD_NRD_1	82	84	PF00675	0.555
CLV_PCSK_KEX2_1	250	252	PF00082	0.673
CLV_PCSK_KEX2_1	288	290	PF00082	0.669
CLV_PCSK_KEX2_1	562	564	PF00082	0.510
CLV_PCSK_KEX2_1	582	584	PF00082	0.248
CLV_PCSK_KEX2_1	644	646	PF00082	0.467
CLV_PCSK_KEX2_1	649	651	PF00082	0.389
CLV_PCSK_KEX2_1	82	84	PF00082	0.575
CLV_PCSK_PC1ET2_1	288	290	PF00082	0.677
CLV_PCSK_PC7_1	640	646	PF00082	0.455
CLV_PCSK_SKI1_1	17	21	PF00082	0.383
CLV_PCSK_SKI1_1	179	183	PF00082	0.472
CLV_PCSK_SKI1_1	316	320	PF00082	0.668
CLV_PCSK_SKI1_1	86	90	PF00082	0.601
DEG_APCC_DBOX_1	315	323	PF00400	0.663
DEG_MDM2_SWIB_1	181	188	PF02201	0.466
DEG_SCF_FBW7_1	657	664	PF00400	0.468
DOC_ANK_TNKS_1	535	542	PF00023	0.518
DOC_CKS1_1	635	640	PF01111	0.410
DOC_MAPK_DCC_7	250	260	PF00069	0.583
DOC_MAPK_FxFP_2	32	35	PF00069	0.557
DOC_MAPK_FxFP_2	461	464	PF00069	0.411
DOC_MAPK_gen_1	582	590	PF00069	0.535
DOC_MAPK_gen_1	649	659	PF00069	0.428
DOC_MAPK_JIP1_4	542	548	PF00069	0.366
DOC_MAPK_MEF2A_6	251	260	PF00069	0.572
DOC_MAPK_MEF2A_6	530	537	PF00069	0.413
DOC_MAPK_MEF2A_6	583	592	PF00069	0.512
DOC_MAPK_MEF2A_6	689	696	PF00069	0.487
DOC_MAPK_MEF2A_6	701	709	PF00069	0.384
DOC_PP1_RVXF_1	602	609	PF00149	0.379
DOC_PP2B_LxvP_1	99	102	PF13499	0.799
DOC_PP4_FxxP_1	32	35	PF00568	0.522
DOC_PP4_FxxP_1	461	464	PF00568	0.395
DOC_PP4_FxxP_1	548	551	PF00568	0.420
DOC_USP7_MATH_1	115	119	PF00917	0.771
DOC_USP7_MATH_1	203	207	PF00917	0.545
DOC_USP7_MATH_1	240	244	PF00917	0.609
DOC_USP7_MATH_1	296	300	PF00917	0.700
DOC_USP7_MATH_1	302	306	PF00917	0.749
DOC_USP7_MATH_1	330	334	PF00917	0.566
DOC_USP7_MATH_1	409	413	PF00917	0.525
DOC_USP7_MATH_1	593	597	PF00917	0.494
DOC_WW_Pin1_4	160	165	PF00397	0.424
DOC_WW_Pin1_4	433	438	PF00397	0.405
DOC_WW_Pin1_4	473	478	PF00397	0.750
DOC_WW_Pin1_4	505	510	PF00397	0.549
DOC_WW_Pin1_4	547	552	PF00397	0.406
DOC_WW_Pin1_4	634	639	PF00397	0.406
DOC_WW_Pin1_4	657	662	PF00397	0.452
DOC_WW_Pin1_4	688	693	PF00397	0.466
DOC_WW_Pin1_4	74	79	PF00397	0.533
LIG_14-3-3_CanoR_1	179	184	PF00244	0.392
LIG_14-3-3_CanoR_1	250	258	PF00244	0.599
LIG_14-3-3_CanoR_1	27	31	PF00244	0.470
LIG_14-3-3_CanoR_1	289	298	PF00244	0.662
LIG_14-3-3_CanoR_1	341	349	PF00244	0.535
LIG_14-3-3_CanoR_1	362	369	PF00244	0.555
LIG_14-3-3_CanoR_1	431	440	PF00244	0.501
LIG_14-3-3_CanoR_1	555	561	PF00244	0.481
LIG_14-3-3_CanoR_1	582	590	PF00244	0.442
LIG_14-3-3_CanoR_1	594	600	PF00244	0.447
LIG_14-3-3_CanoR_1	644	648	PF00244	0.462
LIG_14-3-3_CanoR_1	652	658	PF00244	0.403
LIG_APCC_ABBA_1	258	263	PF00400	0.536
LIG_APCC_ABBAyCdc20_2	680	686	PF00400	0.393
LIG_BIR_II_1	1	5	PF00653	0.708
LIG_BIR_III_4	263	267	PF00653	0.485
LIG_BRCT_BRCA1_1	179	183	PF00533	0.361
LIG_BRCT_BRCA1_1	28	32	PF00533	0.474
LIG_BRCT_BRCA1_1	435	439	PF00533	0.419
LIG_Clathr_ClatBox_1	668	672	PF01394	0.405
LIG_CtBP_PxDLS_1	44	48	PF00389	0.512
LIG_eIF4E_1	145	151	PF01652	0.476
LIG_FHA_1	170	176	PF00498	0.505
LIG_FHA_1	250	256	PF00498	0.635
LIG_FHA_1	293	299	PF00498	0.724
LIG_FHA_1	343	349	PF00498	0.536
LIG_FHA_1	362	368	PF00498	0.486
LIG_FHA_1	506	512	PF00498	0.522
LIG_FHA_1	587	593	PF00498	0.469
LIG_FHA_1	689	695	PF00498	0.435
LIG_FHA_1	700	706	PF00498	0.346
LIG_FHA_2	196	202	PF00498	0.627
LIG_FHA_2	266	272	PF00498	0.605
LIG_FHA_2	555	561	PF00498	0.468
LIG_FHA_2	582	588	PF00498	0.449
LIG_FHA_2	92	98	PF00498	0.733
LIG_FXI_DFP_1	714	718	PF00024	0.412
LIG_LIR_Apic_2	29	35	PF02991	0.504
LIG_LIR_Apic_2	374	378	PF02991	0.394
LIG_LIR_Apic_2	547	551	PF02991	0.416
LIG_LIR_Gen_1	135	144	PF02991	0.500
LIG_LIR_Gen_1	180	189	PF02991	0.348
LIG_LIR_Gen_1	502	512	PF02991	0.633
LIG_LIR_Nem_3	135	139	PF02991	0.508
LIG_LIR_Nem_3	180	186	PF02991	0.348
LIG_LIR_Nem_3	190	195	PF02991	0.405
LIG_LIR_Nem_3	214	219	PF02991	0.474
LIG_LIR_Nem_3	502	507	PF02991	0.668
LIG_LIR_Nem_3	664	669	PF02991	0.489
LIG_MYND_1	467	471	PF01753	0.605
LIG_NRBOX	693	699	PF00104	0.401
LIG_Pex14_2	181	185	PF04695	0.466
LIG_Pex14_2	544	548	PF04695	0.394
LIG_Rb_pABgroove_1	26	34	PF01858	0.546
LIG_SH2_CRK	369	373	PF00017	0.364
LIG_SH2_CRK	375	379	PF00017	0.383
LIG_SH2_CRK	603	607	PF00017	0.374
LIG_SH2_NCK_1	369	373	PF00017	0.399
LIG_SH2_NCK_1	375	379	PF00017	0.418
LIG_SH2_SRC	261	264	PF00017	0.529
LIG_SH2_SRC	373	376	PF00017	0.387
LIG_SH2_STAP1	132	136	PF00017	0.517
LIG_SH2_STAP1	166	170	PF00017	0.589
LIG_SH2_STAP1	369	373	PF00017	0.380
LIG_SH2_STAT5	192	195	PF00017	0.543
LIG_SH2_STAT5	231	234	PF00017	0.417
LIG_SH2_STAT5	261	264	PF00017	0.455
LIG_SH2_STAT5	358	361	PF00017	0.466
LIG_SH2_STAT5	379	382	PF00017	0.406
LIG_SH2_STAT5	421	424	PF00017	0.468
LIG_SH2_STAT5	678	681	PF00017	0.388
LIG_SH2_STAT5	84	87	PF00017	0.553
LIG_SH3_2	477	482	PF14604	0.584
LIG_SH3_3	454	460	PF00018	0.426
LIG_SH3_3	461	467	PF00018	0.483
LIG_SH3_3	474	480	PF00018	0.691
LIG_SH3_3	49	55	PF00018	0.690
LIG_SUMO_SIM_par_1	491	496	PF11976	0.688
LIG_SUMO_SIM_par_1	667	673	PF11976	0.449
LIG_TRAF2_1	393	396	PF00917	0.682
LIG_TRAF2_1	449	452	PF00917	0.364
LIG_TYR_ITIM	367	372	PF00017	0.371
LIG_WRC_WIRS_1	445	450	PF05994	0.497
LIG_WW_3	35	39	PF00397	0.568
MOD_CDC14_SPxK_1	550	553	PF00782	0.423
MOD_CDK_SPK_2	74	79	PF00069	0.504
MOD_CDK_SPxK_1	547	553	PF00069	0.411
MOD_CDK_SPxK_1	634	640	PF00069	0.411
MOD_CK1_1	10	16	PF00069	0.659
MOD_CK1_1	242	248	PF00069	0.564
MOD_CK1_1	301	307	PF00069	0.756
MOD_CK1_1	328	334	PF00069	0.545
MOD_CK1_1	46	52	PF00069	0.737
MOD_CK1_1	510	516	PF00069	0.564
MOD_CK1_1	53	59	PF00069	0.633
MOD_CK1_1	567	573	PF00069	0.473
MOD_CK2_1	195	201	PF00069	0.605
MOD_CK2_1	446	452	PF00069	0.397
MOD_CK2_1	567	573	PF00069	0.486
MOD_GlcNHglycan	1	4	PF01048	0.727
MOD_GlcNHglycan	244	247	PF01048	0.555
MOD_GlcNHglycan	298	301	PF01048	0.681
MOD_GlcNHglycan	327	330	PF01048	0.624
MOD_GlcNHglycan	355	358	PF01048	0.615
MOD_GlcNHglycan	48	51	PF01048	0.726
MOD_GlcNHglycan	488	491	PF01048	0.724
MOD_GlcNHglycan	52	55	PF01048	0.697
MOD_GlcNHglycan	526	529	PF01048	0.395
MOD_GlcNHglycan	79	82	PF01048	0.433
MOD_GlcNHglycan	9	12	PF01048	0.663
MOD_GSK3_1	111	118	PF00069	0.766
MOD_GSK3_1	165	172	PF00069	0.436
MOD_GSK3_1	177	184	PF00069	0.364
MOD_GSK3_1	292	299	PF00069	0.677
MOD_GSK3_1	46	53	PF00069	0.694
MOD_GSK3_1	507	514	PF00069	0.588
MOD_GSK3_1	564	571	PF00069	0.448
MOD_GSK3_1	653	660	PF00069	0.430
MOD_GSK3_1	670	677	PF00069	0.377
MOD_N-GLC_1	177	182	PF02516	0.449
MOD_N-GLC_1	360	365	PF02516	0.559
MOD_N-GLC_1	431	436	PF02516	0.522
MOD_NEK2_1	181	186	PF00069	0.414
MOD_NEK2_1	195	200	PF00069	0.529
MOD_NEK2_1	26	31	PF00069	0.406
MOD_NEK2_1	325	330	PF00069	0.581
MOD_NEK2_1	415	420	PF00069	0.371
MOD_NEK2_1	422	427	PF00069	0.363
MOD_NEK2_1	564	569	PF00069	0.511
MOD_NEK2_1	653	658	PF00069	0.436
MOD_NEK2_1	687	692	PF00069	0.436
MOD_NEK2_2	409	414	PF00069	0.443
MOD_NEK2_2	586	591	PF00069	0.482
MOD_PIKK_1	250	256	PF00454	0.562
MOD_PIKK_1	431	437	PF00454	0.403
MOD_PIKK_1	670	676	PF00454	0.367
MOD_PKA_1	250	256	PF00069	0.426
MOD_PKA_2	249	255	PF00069	0.617
MOD_PKA_2	26	32	PF00069	0.386
MOD_PKA_2	325	331	PF00069	0.580
MOD_PKA_2	361	367	PF00069	0.584
MOD_PKA_2	401	407	PF00069	0.621
MOD_PKA_2	409	415	PF00069	0.522
MOD_PKA_2	554	560	PF00069	0.543
MOD_PKA_2	581	587	PF00069	0.435
MOD_PKA_2	593	599	PF00069	0.460
MOD_PKA_2	643	649	PF00069	0.465
MOD_Plk_1	177	183	PF00069	0.437
MOD_Plk_1	56	62	PF00069	0.621
MOD_Plk_1	564	570	PF00069	0.500
MOD_Plk_1	586	592	PF00069	0.458
MOD_Plk_2-3	554	560	PF00069	0.508
MOD_Plk_4	132	138	PF00069	0.503
MOD_Plk_4	204	210	PF00069	0.517
MOD_Plk_4	26	32	PF00069	0.387
MOD_Plk_4	330	336	PF00069	0.554
MOD_Plk_4	367	373	PF00069	0.398
MOD_Plk_4	674	680	PF00069	0.390
MOD_ProDKin_1	160	166	PF00069	0.433
MOD_ProDKin_1	433	439	PF00069	0.406
MOD_ProDKin_1	473	479	PF00069	0.753
MOD_ProDKin_1	505	511	PF00069	0.542
MOD_ProDKin_1	547	553	PF00069	0.411
MOD_ProDKin_1	634	640	PF00069	0.411
MOD_ProDKin_1	657	663	PF00069	0.455
MOD_ProDKin_1	688	694	PF00069	0.461
MOD_ProDKin_1	74	80	PF00069	0.528
MOD_SUMO_for_1	209	212	PF00179	0.563
MOD_SUMO_rev_2	596	600	PF00179	0.549
TRG_DiLeu_BaEn_2	539	545	PF01217	0.535
TRG_DiLeu_BaLyEn_6	454	459	PF01217	0.440
TRG_DiLeu_BaLyEn_6	649	654	PF01217	0.542
TRG_ENDOCYTIC_2	145	148	PF00928	0.346
TRG_ENDOCYTIC_2	192	195	PF00928	0.543
TRG_ENDOCYTIC_2	369	372	PF00928	0.362
TRG_ENDOCYTIC_2	426	429	PF00928	0.383
TRG_ENDOCYTIC_2	603	606	PF00928	0.380
TRG_ER_diArg_1	224	227	PF00400	0.385
TRG_ER_diArg_1	249	251	PF00400	0.447
TRG_ER_diArg_1	561	563	PF00400	0.507
TRG_ER_diArg_1	581	583	PF00400	0.239
TRG_ER_diArg_1	649	652	PF00400	0.435
TRG_ER_diArg_1	82	84	PF00400	0.572
TRG_NES_CRM1_1	211	223	PF08389	0.521
TRG_NES_CRM1_1	703	718	PF08389	0.419
TRG_NLS_MonoExtN_4	285	292	PF00514	0.694
TRG_Pf-PMV_PEXEL_1	536	540	PF00026	0.496
TRG_Pf-PMV_PEXEL_1	611	615	PF00026	0.447
TRG_Pf-PMV_PEXEL_1	629	634	PF00026	0.536

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HW55	Leptomonas seymouri	67%	96%
A0A0S4IV39	Bodo saltans	37%	90%
A0A1X0NVE2	Trypanosomatidae	45%	100%
A0A3S5H6I6	Leishmania donovani	100%	100%
A0A422MV99	Trypanosoma rangeli	45%	100%
A4H6H8	Leishmania braziliensis	83%	100%
D0A7I9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E9ANJ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
Q4QH27	Leishmania major	95%	100%
V5BW97	Trypanosoma cruzi	45%	100%