Leishmanolysin

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Leishmanolysin

Gene product:

GP63 - leishmanolysin

Species:

Leishmania infantum

UniProt:

A4HUG0_LEIIN

TriTrypDb:

LINF_100011300

Length:

763

Annotations

LeishMANIAdb annotations

Publication identifier(s): 31356625
GPI-anchored cell surface protease. Broad-spectrum ectoenzyme involved in pathogenesis. Heavily expanded family in all parazitic species.. Localization: Cell surface (experimental)

LeishMANIAdb interaction annotations

by homology
Contact email: albert.descoteaux@iaf.inrs.ca
Publication title: The host cell secretory pathway mediates the export of Leishmania virulence factors out of the parasitophorous vacuole
Publication 1st author(s): Amandine Isnard
Publication Identifier(s): 25826301
Host organism: -1
Interaction detection method(s): protease assay
Interaction type: physical association
Identification method participant A: monoclonal antibody western blot
Identification method participant B: monoclonal antibody western blot
ID(s) interactor A: P05627
ID(s) interactor B: P08148
Taxid interactor A: Mus musculus
Taxid interactor B: Leishmania major
Biological role(s) interactor A: enzyme
Biological role(s) interactor B: enzyme target
Experimental role(s) interactor A: neutral component

Annotations by Jardim et al.

Plasma membrane, GP63, leishmanolysin GP63-3

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	yes	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 48

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 41, no: 6
NetGPI	no	yes: 0, no: 47

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	48
GO:0110165	cellular anatomical entity	1	48
GO:0005737	cytoplasm	2	4
GO:0018995	host cellular component	2	4
GO:0033643	host cell part	3	4
GO:0033646	host intracellular part	4	4
GO:0033647	host intracellular organelle	5	4
GO:0033648	host intracellular membrane-bounded organelle	6	4
GO:0042025	host cell nucleus	7	4

Expansion

Sequence features

A4HUG0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HUG0

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	48
GO:0006807	nitrogen compound metabolic process	2	48
GO:0007155	cell adhesion	2	48
GO:0008152	metabolic process	1	48
GO:0009987	cellular process	1	48
GO:0019538	protein metabolic process	3	48
GO:0043170	macromolecule metabolic process	3	48
GO:0044238	primary metabolic process	2	48
GO:0071704	organic substance metabolic process	2	48
GO:1901564	organonitrogen compound metabolic process	3	48
GO:0009966	regulation of signal transduction	4	4
GO:0010646	regulation of cell communication	4	4
GO:0010749	regulation of nitric oxide mediated signal transduction	6	4
GO:0023051	regulation of signaling	3	4
GO:0035821	modulation of process of another organism	2	4
GO:0044003	modulation by symbiont of host process	3	4
GO:0044068	modulation by symbiont of host cellular process	4	4
GO:0044081	modulation by symbiont of host nitric oxide-mediated signal transduction	5	4
GO:0044403	biological process involved in symbiotic interaction	2	4
GO:0044419	biological process involved in interspecies interaction between organisms	1	4
GO:0044501	modulation of signal transduction in another organism	3	4
GO:0048583	regulation of response to stimulus	3	4
GO:0050789	regulation of biological process	2	4
GO:0050794	regulation of cellular process	3	4
GO:0051701	biological process involved in interaction with host	3	4
GO:0052027	modulation by symbiont of host signal transduction pathway	4	4
GO:0065007	biological regulation	1	4
GO:0075130	modulation by symbiont of host protein kinase-mediated signal transduction	5	4
GO:1902531	regulation of intracellular signal transduction	5	4

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	48
GO:0004175	endopeptidase activity	4	48
GO:0004222	metalloendopeptidase activity	5	48
GO:0005488	binding	1	48
GO:0008233	peptidase activity	3	48
GO:0008237	metallopeptidase activity	4	48
GO:0016787	hydrolase activity	2	48
GO:0043167	ion binding	2	48
GO:0043169	cation binding	3	48
GO:0046872	metal ion binding	4	48
GO:0140096	catalytic activity, acting on a protein	2	48
GO:0008047	enzyme activator activity	3	4
GO:0008160	protein tyrosine phosphatase activator activity	6	4
GO:0019208	phosphatase regulator activity	3	4
GO:0019211	phosphatase activator activity	4	4
GO:0019888	protein phosphatase regulator activity	4	4
GO:0030234	enzyme regulator activity	2	4
GO:0072542	protein phosphatase activator activity	5	4
GO:0098772	molecular function regulator activity	1	4
GO:0140677	molecular function activator activity	2	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	136	138	PF00675	0.444
CLV_NRD_NRD_1	24	26	PF00675	0.454
CLV_NRD_NRD_1	253	255	PF00675	0.486
CLV_NRD_NRD_1	44	46	PF00675	0.438
CLV_NRD_NRD_1	451	453	PF00675	0.434
CLV_NRD_NRD_1	48	50	PF00675	0.467
CLV_NRD_NRD_1	743	745	PF00675	0.717
CLV_PCSK_KEX2_1	136	138	PF00082	0.444
CLV_PCSK_KEX2_1	253	255	PF00082	0.486
CLV_PCSK_KEX2_1	313	315	PF00082	0.621
CLV_PCSK_KEX2_1	44	46	PF00082	0.440
CLV_PCSK_KEX2_1	451	453	PF00082	0.440
CLV_PCSK_KEX2_1	48	50	PF00082	0.467
CLV_PCSK_KEX2_1	743	745	PF00082	0.717
CLV_PCSK_PC1ET2_1	313	315	PF00082	0.613
CLV_PCSK_PC7_1	44	50	PF00082	0.448
CLV_PCSK_SKI1_1	314	318	PF00082	0.599
CLV_PCSK_SKI1_1	421	425	PF00082	0.606
CLV_PCSK_SKI1_1	48	52	PF00082	0.465
CLV_PCSK_SKI1_1	557	561	PF00082	0.514
DEG_APCC_DBOX_1	700	708	PF00400	0.373
DEG_Nend_UBRbox_1	1	4	PF02207	0.661
DEG_SCF_TRCP1_1	297	302	PF00400	0.234
DEG_SPOP_SBC_1	167	171	PF00917	0.740
DEG_SPOP_SBC_1	86	90	PF00917	0.644
DOC_CKS1_1	83	88	PF01111	0.639
DOC_CYCLIN_yCln2_LP_2	279	285	PF00134	0.334
DOC_CYCLIN_yCln2_LP_2	505	511	PF00134	0.345
DOC_MAPK_gen_1	133	143	PF00069	0.632
DOC_MAPK_gen_1	158	167	PF00069	0.629
DOC_MAPK_gen_1	23	33	PF00069	0.644
DOC_MAPK_gen_1	451	459	PF00069	0.384
DOC_MAPK_MEF2A_6	136	145	PF00069	0.628
DOC_PP2B_LxvP_1	109	112	PF13499	0.640
DOC_PP2B_LxvP_1	123	126	PF13499	0.649
DOC_PP2B_LxvP_1	505	508	PF13499	0.429
DOC_PP2B_LxvP_1	62	65	PF13499	0.638
DOC_PP4_FxxP_1	355	358	PF00568	0.449
DOC_PP4_FxxP_1	601	604	PF00568	0.317
DOC_PP4_FxxP_1	92	95	PF00568	0.649
DOC_USP7_MATH_1	65	69	PF00917	0.625
DOC_USP7_MATH_1	657	661	PF00917	0.341
DOC_USP7_MATH_1	86	90	PF00917	0.647
DOC_WW_Pin1_4	107	112	PF00397	0.636
DOC_WW_Pin1_4	149	154	PF00397	0.618
DOC_WW_Pin1_4	694	699	PF00397	0.451
DOC_WW_Pin1_4	82	87	PF00397	0.639
DOC_WW_Pin1_4	91	96	PF00397	0.652
LIG_14-3-3_CanoR_1	163	168	PF00244	0.710
LIG_14-3-3_CanoR_1	174	179	PF00244	0.751
LIG_14-3-3_CanoR_1	220	226	PF00244	0.427
LIG_14-3-3_CanoR_1	227	232	PF00244	0.357
LIG_14-3-3_CanoR_1	271	279	PF00244	0.437
LIG_14-3-3_CanoR_1	34	41	PF00244	0.584
LIG_14-3-3_CanoR_1	451	460	PF00244	0.257
LIG_14-3-3_CanoR_1	48	53	PF00244	0.661
LIG_14-3-3_CanoR_1	580	584	PF00244	0.352
LIG_14-3-3_CanoR_1	630	638	PF00244	0.295
LIG_14-3-3_CanoR_1	656	664	PF00244	0.337
LIG_14-3-3_CanoR_1	681	686	PF00244	0.435
LIG_14-3-3_CanoR_1	69	76	PF00244	0.624
LIG_14-3-3_CanoR_1	747	755	PF00244	0.507
LIG_14-3-3_CanoR_1	8	15	PF00244	0.643
LIG_APCC_ABBA_1	326	331	PF00400	0.451
LIG_APCC_ABBA_1	520	525	PF00400	0.424
LIG_APCC_ABBA_1	619	624	PF00400	0.320
LIG_APCC_ABBA_1	641	646	PF00400	0.451
LIG_BRCT_BRCA1_1	103	107	PF00533	0.638
LIG_BRCT_BRCA1_1	433	437	PF00533	0.377
LIG_BRCT_BRCA1_1	67	71	PF00533	0.624
LIG_BRCT_BRCA1_1	707	711	PF00533	0.451
LIG_BRCT_BRCA1_1	88	92	PF00533	0.645
LIG_FHA_1	116	122	PF00498	0.641
LIG_FHA_1	274	280	PF00498	0.375
LIG_FHA_1	328	334	PF00498	0.371
LIG_FHA_1	361	367	PF00498	0.336
LIG_FHA_1	388	394	PF00498	0.348
LIG_FHA_1	4	10	PF00498	0.638
LIG_FHA_1	418	424	PF00498	0.400
LIG_FHA_1	452	458	PF00498	0.265
LIG_FHA_1	517	523	PF00498	0.441
LIG_FHA_1	576	582	PF00498	0.425
LIG_FHA_1	680	686	PF00498	0.435
LIG_FHA_1	695	701	PF00498	0.279
LIG_FHA_1	749	755	PF00498	0.476
LIG_FHA_2	302	308	PF00498	0.377
LIG_FHA_2	343	349	PF00498	0.283
LIG_GBD_Chelix_1	141	149	PF00786	0.420
LIG_GBD_Chelix_1	755	763	PF00786	0.689
LIG_LIR_Apic_2	353	358	PF02991	0.428
LIG_LIR_Apic_2	80	86	PF02991	0.631
LIG_LIR_Apic_2	89	95	PF02991	0.646
LIG_LIR_Gen_1	413	423	PF02991	0.400
LIG_LIR_Gen_1	477	485	PF02991	0.446
LIG_LIR_Nem_3	369	374	PF02991	0.425
LIG_LIR_Nem_3	413	418	PF02991	0.400
LIG_LIR_Nem_3	477	483	PF02991	0.408
LIG_LIR_Nem_3	666	670	PF02991	0.358
LIG_MYND_1	592	596	PF01753	0.283
LIG_MYND_1	99	103	PF01753	0.623
LIG_PCNA_yPIPBox_3	414	423	PF02747	0.238
LIG_PDZ_Class_2	758	763	PF00595	0.478
LIG_Pex14_1	562	566	PF04695	0.439
LIG_Pex14_2	433	437	PF04695	0.386
LIG_PTB_Apo_2	365	372	PF02174	0.435
LIG_SH2_CRK	415	419	PF00017	0.396
LIG_SH2_CRK	47	51	PF00017	0.657
LIG_SH2_CRK	515	519	PF00017	0.451
LIG_SH2_CRK	667	671	PF00017	0.411
LIG_SH2_CRK	83	87	PF00017	0.637
LIG_SH2_GRB2like	693	696	PF00017	0.338
LIG_SH2_NCK_1	83	87	PF00017	0.637
LIG_SH2_PTP2	242	245	PF00017	0.341
LIG_SH2_PTP2	722	725	PF00017	0.447
LIG_SH2_SRC	480	483	PF00017	0.340
LIG_SH2_SRC	722	725	PF00017	0.447
LIG_SH2_STAP1	683	687	PF00017	0.451
LIG_SH2_STAT3	683	686	PF00017	0.435
LIG_SH2_STAT5	242	245	PF00017	0.425
LIG_SH2_STAT5	374	377	PF00017	0.429
LIG_SH2_STAT5	480	483	PF00017	0.414
LIG_SH2_STAT5	600	603	PF00017	0.434
LIG_SH2_STAT5	716	719	PF00017	0.447
LIG_SH2_STAT5	722	725	PF00017	0.381
LIG_SH2_STAT5	83	86	PF00017	0.633
LIG_SH3_2	153	158	PF14604	0.615
LIG_SH3_3	108	114	PF00018	0.630
LIG_SH3_3	119	125	PF00018	0.657
LIG_SH3_3	150	156	PF00018	0.618
LIG_SH3_3	235	241	PF00018	0.338
LIG_SH3_3	401	407	PF00018	0.254
LIG_SH3_3	589	595	PF00018	0.296
LIG_SH3_3	615	621	PF00018	0.314
LIG_SH3_3	64	70	PF00018	0.625
LIG_SH3_3	92	98	PF00018	0.644
LIG_Sin3_3	750	757	PF02671	0.487
LIG_SxIP_EBH_1	32	45	PF03271	0.604
LIG_TRAF2_1	379	382	PF00917	0.322
LIG_TRAF2_1	710	713	PF00917	0.300
LIG_TRFH_1	107	111	PF08558	0.521
LIG_TYR_ITIM	240	245	PF00017	0.387
LIG_TYR_ITIM	478	483	PF00017	0.437
LIG_WW_2	125	128	PF00397	0.529
MOD_CDK_SPxxK_3	694	701	PF00069	0.464
MOD_CK1_1	110	116	PF00069	0.510
MOD_CK1_1	166	172	PF00069	0.696
MOD_CK1_1	257	263	PF00069	0.397
MOD_CK1_1	298	304	PF00069	0.237
MOD_CK1_1	377	383	PF00069	0.527
MOD_CK1_1	491	497	PF00069	0.471
MOD_CK1_1	516	522	PF00069	0.338
MOD_CK1_1	579	585	PF00069	0.354
MOD_CK1_1	705	711	PF00069	0.414
MOD_CK1_1	72	78	PF00069	0.503
MOD_CK1_1	91	97	PF00069	0.524
MOD_CK2_1	531	537	PF00069	0.541
MOD_CK2_1	706	712	PF00069	0.360
MOD_Cter_Amidation	741	744	PF01082	0.632
MOD_DYRK1A_RPxSP_1	93	97	PF00069	0.525
MOD_GlcNHglycan	235	238	PF01048	0.511
MOD_GlcNHglycan	256	259	PF01048	0.365
MOD_GlcNHglycan	297	300	PF01048	0.248
MOD_GlcNHglycan	490	493	PF01048	0.472
MOD_GlcNHglycan	515	518	PF01048	0.412
MOD_GlcNHglycan	609	612	PF01048	0.405
MOD_GlcNHglycan	690	693	PF01048	0.471
MOD_GlcNHglycan	708	711	PF01048	0.261
MOD_GlcNHglycan	79	82	PF01048	0.506
MOD_GlcNHglycan	9	12	PF01048	0.527
MOD_GSK3_1	101	108	PF00069	0.521
MOD_GSK3_1	163	170	PF00069	0.527
MOD_GSK3_1	273	280	PF00069	0.375
MOD_GSK3_1	291	298	PF00069	0.244
MOD_GSK3_1	3	10	PF00069	0.524
MOD_GSK3_1	360	367	PF00069	0.531
MOD_GSK3_1	417	424	PF00069	0.433
MOD_GSK3_1	427	434	PF00069	0.329
MOD_GSK3_1	48	55	PF00069	0.554
MOD_GSK3_1	575	582	PF00069	0.390
MOD_GSK3_1	65	72	PF00069	0.500
MOD_GSK3_1	675	682	PF00069	0.390
MOD_GSK3_1	702	709	PF00069	0.404
MOD_GSK3_1	77	84	PF00069	0.507
MOD_GSK3_1	87	94	PF00069	0.535
MOD_N-GLC_1	557	562	PF02516	0.455
MOD_N-GLC_1	694	699	PF02516	0.385
MOD_NEK2_1	105	110	PF00069	0.531
MOD_NEK2_1	17	22	PF00069	0.533
MOD_NEK2_1	427	432	PF00069	0.454
MOD_NEK2_1	71	76	PF00069	0.505
MOD_NEK2_2	29	34	PF00069	0.517
MOD_PIKK_1	3	9	PF00454	0.524
MOD_PIKK_1	39	45	PF00454	0.501
MOD_PIKK_1	57	63	PF00454	0.536
MOD_PK_1	163	169	PF00069	0.599
MOD_PK_1	174	180	PF00069	0.676
MOD_PK_1	681	687	PF00069	0.423
MOD_PKA_1	451	457	PF00069	0.237
MOD_PKA_1	48	54	PF00069	0.556
MOD_PKA_2	221	227	PF00069	0.382
MOD_PKA_2	291	297	PF00069	0.311
MOD_PKA_2	451	457	PF00069	0.237
MOD_PKA_2	48	54	PF00069	0.556
MOD_PKA_2	579	585	PF00069	0.401
MOD_PKA_2	629	635	PF00069	0.325
MOD_PKA_2	7	13	PF00069	0.526
MOD_Plk_1	439	445	PF00069	0.293
MOD_Plk_1	570	576	PF00069	0.373
MOD_Plk_1	702	708	PF00069	0.396
MOD_Plk_2-3	307	313	PF00069	0.440
MOD_Plk_4	257	263	PF00069	0.344
MOD_Plk_4	439	445	PF00069	0.346
MOD_ProDKin_1	107	113	PF00069	0.520
MOD_ProDKin_1	149	155	PF00069	0.497
MOD_ProDKin_1	694	700	PF00069	0.541
MOD_ProDKin_1	82	88	PF00069	0.527
MOD_ProDKin_1	91	97	PF00069	0.535
MOD_SUMO_rev_2	348	358	PF00179	0.393
TRG_ENDOCYTIC_2	242	245	PF00928	0.387
TRG_ENDOCYTIC_2	415	418	PF00928	0.481
TRG_ENDOCYTIC_2	47	50	PF00928	0.550
TRG_ENDOCYTIC_2	480	483	PF00928	0.488
TRG_ENDOCYTIC_2	515	518	PF00928	0.541
TRG_ENDOCYTIC_2	667	670	PF00928	0.515
TRG_ENDOCYTIC_2	722	725	PF00928	0.535
TRG_ER_diArg_1	253	255	PF00400	0.310
TRG_ER_diArg_1	450	452	PF00400	0.267
TRG_ER_diArg_1	47	49	PF00400	0.548
TRG_Pf-PMV_PEXEL_1	211	215	PF00026	0.358
TRG_Pf-PMV_PEXEL_1	48	52	PF00026	0.558

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IL11	Leptomonas seymouri	35%	100%
A0A0S4IN60	Bodo saltans	32%	85%
A0A0S4IYM3	Bodo saltans	29%	100%
A0A1X0NDU8	Trypanosomatidae	27%	100%
A0A1X0NFL1	Trypanosomatidae	27%	81%
A0A1X0NFT1	Trypanosomatidae	26%	83%
A0A1X0NI38	Trypanosomatidae	27%	87%
A0A1X0NI74	Trypanosomatidae	27%	100%
A0A1X0NII4	Trypanosomatidae	28%	91%
A0A1X0NK29	Trypanosomatidae	30%	83%
A0A1X0NMK3	Trypanosomatidae	26%	100%
A0A1X0NRY8	Trypanosomatidae	33%	100%
A0A1X0NVC3	Trypanosomatidae	29%	100%
A0A1X0NX98	Trypanosomatidae	30%	95%
A0A1X0NXH6	Trypanosomatidae	32%	100%
A0A1X0NXR7	Trypanosomatidae	32%	100%
A0A1X0NYE3	Trypanosomatidae	29%	100%
A0A1X0NZN6	Trypanosomatidae	28%	100%
A0A1X0P3K0	Trypanosomatidae	28%	100%
A0A1X0P5J0	Trypanosomatidae	33%	100%
A0A1X0P7R3	Trypanosomatidae	35%	100%
A0A3Q8I8N3	Leishmania donovani	88%	100%
A0A3Q8I8P8	Leishmania donovani	94%	100%
A0A3Q8I8S6	Leishmania donovani	85%	100%
A0A3Q8I8S9	Leishmania donovani	94%	100%
A0A3Q8IAZ8	Leishmania donovani	88%	100%
A0A3Q8IC35	Leishmania donovani	88%	100%
A0A3Q8IC44	Leishmania donovani	90%	100%
A0A3Q8IH61	Leishmania donovani	92%	100%
A0A3Q8IIN4	Leishmania donovani	35%	100%
A0A3R7KLR6	Trypanosoma rangeli	32%	100%
A0A3R7KMY2	Trypanosoma rangeli	34%	100%
A0A3R7LFC4	Trypanosoma rangeli	34%	100%
A0A3R7LFZ4	Trypanosoma rangeli	33%	100%
A0A3R7LWG1	Trypanosoma rangeli	33%	100%
A0A3R7LX11	Trypanosoma rangeli	35%	100%
A0A3R7NTI8	Trypanosoma rangeli	33%	100%
A0A3R7R5R1	Trypanosoma rangeli	34%	100%
A0A3S5H6G0	Leishmania donovani	94%	100%
A0A3S5IQY2	Trypanosoma rangeli	34%	100%
A0A3S7WR43	Leishmania donovani	88%	100%
A0A3S7WR46	Leishmania donovani	93%	100%
A0A3S7WR60	Leishmania donovani	95%	100%
A0A3S7X192	Leishmania donovani	36%	100%
A0A422MVF5	Trypanosoma rangeli	33%	100%
A0A422N361	Trypanosoma rangeli	33%	100%
A4H626	Leishmania braziliensis	59%	100%
A4H627	Leishmania braziliensis	57%	100%
A4H630	Leishmania braziliensis	63%	100%
A4H631	Leishmania braziliensis	59%	100%
A4H633	Leishmania braziliensis	59%	100%
A4H634	Leishmania braziliensis	62%	100%
A4H635	Leishmania braziliensis	62%	100%
A4H637	Leishmania braziliensis	63%	100%
A4H638	Leishmania braziliensis	70%	100%
A4H639	Leishmania braziliensis	66%	100%
A4H640	Leishmania braziliensis	62%	100%
A4H6D3	Leishmania braziliensis	58%	100%
A4H6D5	Leishmania braziliensis	59%	100%
A4H6D7	Leishmania braziliensis	57%	100%
A4H6D8	Leishmania braziliensis	57%	100%
A4H6D9	Leishmania braziliensis	57%	100%
A4H6E0	Leishmania braziliensis	57%	100%
A4H6E1	Leishmania braziliensis	57%	100%
A4H6E2	Leishmania braziliensis	59%	100%
A4H6E3	Leishmania braziliensis	61%	100%
A4H6E5	Leishmania braziliensis	68%	100%
A4HJI2	Leishmania braziliensis	35%	100%
A4HUF6	Leishmania infantum	88%	100%
A4HUF8	Leishmania infantum	95%	100%
A4HUF9	Leishmania infantum	85%	100%
A4I6X5	Leishmania infantum	35%	100%
E9AHH5	Leishmania infantum	36%	100%
E9AN53	Leishmania mexicana (strain MHOM/GT/2001/U1103)	75%	100%
E9AN54	Leishmania mexicana (strain MHOM/GT/2001/U1103)	79%	100%
E9AN55	Leishmania mexicana (strain MHOM/GT/2001/U1103)	76%	100%
E9AN56	Leishmania mexicana (strain MHOM/GT/2001/U1103)	75%	100%
E9AN57	Leishmania mexicana (strain MHOM/GT/2001/U1103)	81%	100%
E9AZL8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
E9B1Z9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
P08148	Leishmania major	83%	100%
P15706	Leishmania chagasi	95%	100%
P23223	Leishmania donovani	87%	100%
P43150	Leishmania mexicana	73%	100%
Q00689	Leishmania guyanensis	63%	100%
Q06031	Crithidia fasciculata	53%	100%
Q27673	Leishmania amazonensis	78%	100%
Q29AK2	Drosophila pseudoobscura pseudoobscura	24%	100%
Q4Q662	Leishmania major	35%	100%
Q4Q8L3	Leishmania major	39%	100%
Q4QHG9	Leishmania major	83%	100%
Q4QHH0	Leishmania major	81%	100%
Q4QHH1	Leishmania major	84%	100%
Q4QHH2	Leishmania major	84%	100%
Q6LA77	Leishmania infantum	95%	100%
Q8MNZ1	Leishmania tropica	81%	100%
Q9VH19	Drosophila melanogaster	23%	100%
V5AII7	Trypanosoma cruzi	37%	100%