Leishmanolysin

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Leishmanolysin

Gene product:

GP63 - leishmanolysin

Species:

Leishmania infantum

UniProt:

A4HUF8_LEIIN

TriTrypDb:

LINF_100010800

Length:

599

Annotations

LeishMANIAdb annotations

Publication identifier(s): 31356625
GPI-anchored cell surface protease. Broad-spectrum ectoenzyme involved in pathogenesis. Heavily expanded family in all parazitic species.. Localization: Cell surface (experimental)

LeishMANIAdb interaction annotations

by homology
Contact email: albert.descoteaux@iaf.inrs.ca
Publication title: The host cell secretory pathway mediates the export of Leishmania virulence factors out of the parasitophorous vacuole
Publication 1st author(s): Amandine Isnard
Publication Identifier(s): 25826301
Host organism: -1
Interaction detection method(s): protease assay
Interaction type: physical association
Identification method participant A: monoclonal antibody western blot
Identification method participant B: monoclonal antibody western blot
ID(s) interactor A: P05627
ID(s) interactor B: P08148
Taxid interactor A: Mus musculus
Taxid interactor B: Leishmania major
Biological role(s) interactor A: enzyme
Biological role(s) interactor B: enzyme target
Experimental role(s) interactor A: neutral component

Annotations by Jardim et al.

Plasma membrane, GP63, leishmanolysin GP63-1

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 51

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 41, no: 9
NetGPI	no	yes: 0, no: 50

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Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	4
GO:0016020	membrane	2	51
GO:0018995	host cellular component	2	4
GO:0033643	host cell part	3	4
GO:0033646	host intracellular part	4	4
GO:0033647	host intracellular organelle	5	4
GO:0033648	host intracellular membrane-bounded organelle	6	4
GO:0042025	host cell nucleus	7	4
GO:0110165	cellular anatomical entity	1	51

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Expansion

Sequence features

A4HUF8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HUF8

Function

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Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	51
GO:0006807	nitrogen compound metabolic process	2	51
GO:0007155	cell adhesion	2	51
GO:0008152	metabolic process	1	51
GO:0009966	regulation of signal transduction	4	4
GO:0009987	cellular process	1	51
GO:0010646	regulation of cell communication	4	4
GO:0010749	regulation of nitric oxide mediated signal transduction	6	4
GO:0019538	protein metabolic process	3	51
GO:0023051	regulation of signaling	3	4

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Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	51
GO:0004175	endopeptidase activity	4	51
GO:0004222	metalloendopeptidase activity	5	51
GO:0005488	binding	1	51
GO:0008047	enzyme activator activity	3	4
GO:0008160	protein tyrosine phosphatase activator activity	6	4
GO:0008233	peptidase activity	3	51
GO:0008237	metallopeptidase activity	4	51
GO:0016787	hydrolase activity	2	51
GO:0019208	phosphatase regulator activity	3	4

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Putative motif mimicry

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Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	131	133	PF00675	0.436
CLV_NRD_NRD_1	579	581	PF00675	0.608
CLV_NRD_NRD_1	89	91	PF00675	0.460
CLV_PCSK_KEX2_1	130	132	PF00082	0.445
CLV_PCSK_KEX2_1	149	151	PF00082	0.595
CLV_PCSK_KEX2_1	579	581	PF00082	0.608
CLV_PCSK_KEX2_1	89	91	PF00082	0.460
CLV_PCSK_PC1ET2_1	130	132	PF00082	0.441
CLV_PCSK_PC1ET2_1	149	151	PF00082	0.584
CLV_PCSK_SKI1_1	150	154	PF00082	0.573

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Homologs

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Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IL11	Leptomonas seymouri	35%	91%
A0A0S4IN60	Bodo saltans	32%	67%
A0A0S4IP65	Bodo saltans	22%	74%
A0A0S4IYM3	Bodo saltans	29%	82%
A0A1X0NDG7	Trypanosomatidae	31%	92%
A0A1X0NDJ3	Trypanosomatidae	28%	83%
A0A1X0NDK2	Trypanosomatidae	33%	100%
A0A1X0NDK7	Trypanosomatidae	28%	84%
A0A1X0NDK9	Trypanosomatidae	29%	100%
A0A1X0NDM7	Trypanosomatidae	29%	93%

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