E3 ubiquitin-protein ligase CHFR

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

FHA domain/Ring finger domain/Zinc finger- C3HC4 type (RING finger) containing protein - putative

Species:

Leishmania infantum

UniProt:

A4HTT2_LEIIN

TriTrypDb:

LINF_090006500

Length:

524

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016604	nuclear body	2	12
GO:0016605	PML body	3	12
GO:0110165	cellular anatomical entity	1	12
GO:0005634	nucleus	5	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0016020	membrane	2	1

Expansion

Sequence features

A4HTT2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HTT2

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0016567	protein ubiquitination	7	12
GO:0019538	protein metabolic process	3	12
GO:0032446	protein modification by small protein conjugation	6	12
GO:0036211	protein modification process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044238	primary metabolic process	2	12
GO:0070647	protein modification by small protein conjugation or removal	5	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:0006508	proteolysis	4	1
GO:0006511	ubiquitin-dependent protein catabolic process	7	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009987	cellular process	1	1
GO:0019941	modification-dependent protein catabolic process	6	1
GO:0043632	modification-dependent macromolecule catabolic process	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0051603	proteolysis involved in protein catabolic process	5	1
GO:1901575	organic substance catabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0003824	catalytic activity	1	1
GO:0004842	ubiquitin-protein transferase activity	4	1
GO:0016740	transferase activity	2	1
GO:0019787	ubiquitin-like protein transferase activity	3	1
GO:0140096	catalytic activity, acting on a protein	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	150	154	PF00656	0.613
CLV_C14_Caspase3-7	277	281	PF00656	0.577
CLV_C14_Caspase3-7	292	296	PF00656	0.520
CLV_C14_Caspase3-7	329	333	PF00656	0.271
CLV_NRD_NRD_1	116	118	PF00675	0.344
CLV_NRD_NRD_1	251	253	PF00675	0.592
CLV_NRD_NRD_1	461	463	PF00675	0.439
CLV_NRD_NRD_1	476	478	PF00675	0.246
CLV_NRD_NRD_1	521	523	PF00675	0.484
CLV_PCSK_KEX2_1	116	118	PF00082	0.395
CLV_PCSK_KEX2_1	251	253	PF00082	0.501
CLV_PCSK_KEX2_1	275	277	PF00082	0.552
CLV_PCSK_KEX2_1	461	463	PF00082	0.439
CLV_PCSK_KEX2_1	476	478	PF00082	0.241
CLV_PCSK_KEX2_1	490	492	PF00082	0.257
CLV_PCSK_KEX2_1	521	523	PF00082	0.476
CLV_PCSK_PC1ET2_1	275	277	PF00082	0.517
CLV_PCSK_PC1ET2_1	490	492	PF00082	0.321
CLV_PCSK_PC1ET2_1	521	523	PF00082	0.476
CLV_PCSK_SKI1_1	117	121	PF00082	0.341
CLV_PCSK_SKI1_1	123	127	PF00082	0.288
CLV_PCSK_SKI1_1	131	135	PF00082	0.161
CLV_PCSK_SKI1_1	187	191	PF00082	0.298
DEG_APCC_DBOX_1	99	107	PF00400	0.391
DOC_CDC14_PxL_1	50	58	PF14671	0.496
DOC_CYCLIN_yCln2_LP_2	38	44	PF00134	0.526
DOC_MAPK_gen_1	116	126	PF00069	0.252
DOC_MAPK_gen_1	302	311	PF00069	0.546
DOC_MAPK_RevD_3	101	117	PF00069	0.384
DOC_PP2B_LxvP_1	33	36	PF13499	0.569
DOC_PP4_FxxP_1	190	193	PF00568	0.343
DOC_PP4_FxxP_1	334	337	PF00568	0.309
DOC_USP7_MATH_1	137	141	PF00917	0.570
DOC_USP7_MATH_1	175	179	PF00917	0.592
DOC_USP7_MATH_1	347	351	PF00917	0.368
DOC_USP7_MATH_1	420	424	PF00917	0.314
DOC_USP7_MATH_1	7	11	PF00917	0.763
DOC_WW_Pin1_4	11	16	PF00397	0.664
DOC_WW_Pin1_4	3	8	PF00397	0.693
DOC_WW_Pin1_4	324	329	PF00397	0.391
DOC_WW_Pin1_4	454	459	PF00397	0.343
LIG_14-3-3_CanoR_1	192	197	PF00244	0.284
LIG_14-3-3_CanoR_1	224	232	PF00244	0.399
LIG_14-3-3_CanoR_1	235	244	PF00244	0.547
LIG_14-3-3_CanoR_1	344	352	PF00244	0.391
LIG_14-3-3_CanoR_1	462	468	PF00244	0.457
LIG_14-3-3_CanoR_1	477	485	PF00244	0.478
LIG_APCC_ABBA_1	388	393	PF00400	0.329
LIG_APCC_ABBA_1	56	61	PF00400	0.332
LIG_BIR_II_1	1	5	PF00653	0.632
LIG_deltaCOP1_diTrp_1	428	437	PF00928	0.217
LIG_EVH1_2	401	405	PF00568	0.343
LIG_FHA_1	180	186	PF00498	0.278
LIG_FHA_1	231	237	PF00498	0.376
LIG_FHA_1	53	59	PF00498	0.432
LIG_FHA_1	96	102	PF00498	0.313
LIG_Integrin_isoDGR_2	114	116	PF01839	0.304
LIG_Integrin_isoDGR_2	158	160	PF01839	0.524
LIG_LIR_Apic_2	188	193	PF02991	0.394
LIG_LIR_Apic_2	332	337	PF02991	0.264
LIG_LIR_Gen_1	162	171	PF02991	0.386
LIG_LIR_Gen_1	395	405	PF02991	0.279
LIG_LIR_Nem_3	162	168	PF02991	0.357
LIG_LIR_Nem_3	386	391	PF02991	0.431
LIG_LIR_Nem_3	395	400	PF02991	0.217
LIG_LIR_Nem_3	466	471	PF02991	0.312
LIG_PCNA_PIPBox_1	412	421	PF02747	0.249
LIG_SH2_CRK	50	54	PF00017	0.515
LIG_SH2_STAT3	163	166	PF00017	0.392
LIG_SH2_STAT5	243	246	PF00017	0.401
LIG_SH2_STAT5	418	421	PF00017	0.249
LIG_SH3_3	396	402	PF00018	0.440
LIG_TRAF2_1	253	256	PF00917	0.489
LIG_UBA3_1	164	169	PF00899	0.457
MOD_CDK_SPK_2	3	8	PF00069	0.606
MOD_CDK_SPxxK_3	11	18	PF00069	0.631
MOD_CDK_SPxxK_3	454	461	PF00069	0.279
MOD_CK1_1	279	285	PF00069	0.606
MOD_CK1_1	3	9	PF00069	0.623
MOD_CK1_1	406	412	PF00069	0.347
MOD_CK2_1	235	241	PF00069	0.426
MOD_Cter_Amidation	114	117	PF01082	0.346
MOD_DYRK1A_RPxSP_1	454	458	PF00069	0.279
MOD_GlcNHglycan	149	152	PF01048	0.577
MOD_GlcNHglycan	177	180	PF01048	0.577
MOD_GlcNHglycan	208	211	PF01048	0.396
MOD_GlcNHglycan	345	348	PF01048	0.329
MOD_GlcNHglycan	405	408	PF01048	0.441
MOD_GlcNHglycan	430	433	PF01048	0.372
MOD_GlcNHglycan	61	64	PF01048	0.487
MOD_GlcNHglycan	85	88	PF01048	0.290
MOD_GSK3_1	104	111	PF00069	0.285
MOD_GSK3_1	175	182	PF00069	0.537
MOD_GSK3_1	270	277	PF00069	0.521
MOD_GSK3_1	291	298	PF00069	0.533
MOD_GSK3_1	3	10	PF00069	0.632
MOD_GSK3_1	343	350	PF00069	0.279
MOD_GSK3_1	380	387	PF00069	0.376
MOD_GSK3_1	79	86	PF00069	0.448
MOD_N-GLC_1	108	113	PF02516	0.249
MOD_NEK2_1	181	186	PF00069	0.298
MOD_NEK2_1	343	348	PF00069	0.425
MOD_NEK2_2	297	302	PF00069	0.545
MOD_NEK2_2	347	352	PF00069	0.279
MOD_NEK2_2	463	468	PF00069	0.343
MOD_OFUCOSY	345	351	PF10250	0.279
MOD_PK_1	192	198	PF00069	0.343
MOD_PKA_2	175	181	PF00069	0.450
MOD_PKA_2	223	229	PF00069	0.450
MOD_PKA_2	230	236	PF00069	0.308
MOD_PKA_2	343	349	PF00069	0.329
MOD_PKA_2	7	13	PF00069	0.672
MOD_Plk_4	108	114	PF00069	0.249
MOD_Plk_4	185	191	PF00069	0.304
MOD_Plk_4	192	198	PF00069	0.266
MOD_Plk_4	420	426	PF00069	0.304
MOD_Plk_4	463	469	PF00069	0.305
MOD_ProDKin_1	11	17	PF00069	0.663
MOD_ProDKin_1	3	9	PF00069	0.693
MOD_ProDKin_1	324	330	PF00069	0.391
MOD_ProDKin_1	454	460	PF00069	0.343
MOD_SUMO_for_1	119	122	PF00179	0.384
MOD_SUMO_for_1	168	171	PF00179	0.488
MOD_SUMO_for_1	435	438	PF00179	0.369
TRG_DiLeu_BaEn_4	255	261	PF01217	0.447
TRG_ENDOCYTIC_2	50	53	PF00928	0.499
TRG_ENDOCYTIC_2	513	516	PF00928	0.435
TRG_ER_diArg_1	116	118	PF00400	0.304
TRG_ER_diArg_1	174	177	PF00400	0.543
TRG_ER_diArg_1	251	253	PF00400	0.578
TRG_ER_diArg_1	475	477	PF00400	0.301
TRG_Pf-PMV_PEXEL_1	477	482	PF00026	0.454

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P377	Leptomonas seymouri	63%	98%
A0A0S4JT63	Bodo saltans	42%	100%
A0A1X0NPC5	Trypanosomatidae	45%	99%
A0A3R7MDE3	Trypanosoma rangeli	44%	96%
A0A3S5H6B1	Leishmania donovani	100%	100%
A4H5J1	Leishmania braziliensis	75%	99%
A5WW08	Danio rerio	29%	82%
D0A993	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	94%
E9AML5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
Q4QI21	Leishmania major	94%	100%
Q5FWP4	Xenopus laevis	30%	84%
Q5RF77	Pongo abelii	28%	92%
Q6P256	Xenopus tropicalis	29%	84%
Q810L3	Mus musculus	29%	79%
Q96EP1	Homo sapiens	30%	79%
V5DJC2	Trypanosoma cruzi	44%	84%