Putative ubiquitin-conjugating enzyme e2

Quick info Annotations Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme E2 - putative

Species:

Leishmania infantum

UniProt:

A4HTB1_LEIIN

TriTrypDb:

LINF_070014700

Length:

182

Annotations

Annotations by Jardim et al.

Protein modification, ubiquitin-conjugating enzyme e2

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A4HTB1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HTB1

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0016567	protein ubiquitination	7	1
GO:0019538	protein metabolic process	3	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044238	primary metabolic process	2	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	8
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12
GO:0004842	ubiquitin-protein transferase activity	4	1
GO:0016740	transferase activity	2	10
GO:0019787	ubiquitin-like protein transferase activity	3	1
GO:0061631	ubiquitin conjugating enzyme activity	5	1
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	1
GO:0140096	catalytic activity, acting on a protein	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_PCSK_KEX2_1	116	118	PF00082	0.359
CLV_PCSK_PC1ET2_1	116	118	PF00082	0.352
CLV_PCSK_SKI1_1	10	14	PF00082	0.372
CLV_PCSK_SKI1_1	129	133	PF00082	0.260
CLV_PCSK_SKI1_1	75	79	PF00082	0.296
CLV_PCSK_SKI1_1	90	94	PF00082	0.212
DEG_APCC_DBOX_1	128	136	PF00400	0.282
DOC_CKS1_1	65	70	PF01111	0.227
DOC_CYCLIN_yCln2_LP_2	86	89	PF00134	0.352
DOC_MAPK_MEF2A_6	96	104	PF00069	0.212
DOC_PP2B_LxvP_1	78	81	PF13499	0.212
DOC_PP2B_LxvP_1	86	89	PF13499	0.212
DOC_USP7_MATH_1	70	74	PF00917	0.262
DOC_WW_Pin1_4	116	121	PF00397	0.212
DOC_WW_Pin1_4	139	144	PF00397	0.236
DOC_WW_Pin1_4	64	69	PF00397	0.227
LIG_FHA_1	104	110	PF00498	0.239
LIG_LIR_Gen_1	158	168	PF02991	0.247
LIG_LIR_Gen_1	56	65	PF02991	0.230
LIG_LIR_Nem_3	158	163	PF02991	0.247
LIG_LIR_Nem_3	56	60	PF02991	0.230
LIG_LIR_Nem_3	73	77	PF02991	0.109
LIG_LIR_Nem_3	83	87	PF02991	0.197
LIG_LYPXL_yS_3	84	87	PF13949	0.212
LIG_PCNA_yPIPBox_3	51	62	PF02747	0.301
LIG_SH2_CRK	160	164	PF00017	0.237
LIG_SH2_CRK	74	78	PF00017	0.247
LIG_SH2_STAP1	160	164	PF00017	0.247
LIG_SH2_STAP1	55	59	PF00017	0.302
LIG_SH2_STAT5	55	58	PF00017	0.336
LIG_SH3_1	62	68	PF00018	0.272
LIG_SH3_2	85	90	PF14604	0.306
LIG_SH3_3	62	68	PF00018	0.227
LIG_SH3_3	82	88	PF00018	0.242
LIG_SH3_3	94	100	PF00018	0.242
LIG_SUMO_SIM_anti_2	56	62	PF11976	0.302
LIG_TRFH_1	84	88	PF08558	0.352
LIG_TYR_ITIM	164	169	PF00017	0.302
MOD_CK1_1	34	40	PF00069	0.307
MOD_CK1_1	45	51	PF00069	0.278
MOD_GSK3_1	116	123	PF00069	0.219
MOD_GSK3_1	33	40	PF00069	0.660
MOD_N-GLC_1	34	39	PF02516	0.457
MOD_N-GLC_2	108	110	PF02516	0.352
MOD_NEK2_1	76	81	PF00069	0.354
MOD_PIKK_1	37	43	PF00454	0.302
MOD_PIKK_1	45	51	PF00454	0.351
MOD_PKA_2	37	43	PF00069	0.310
MOD_Plk_4	159	165	PF00069	0.247
MOD_Plk_4	53	59	PF00069	0.337
MOD_ProDKin_1	116	122	PF00069	0.212
MOD_ProDKin_1	139	145	PF00069	0.236
MOD_ProDKin_1	64	70	PF00069	0.227
TRG_ENDOCYTIC_2	160	163	PF00928	0.221
TRG_ENDOCYTIC_2	166	169	PF00928	0.282
TRG_ENDOCYTIC_2	55	58	PF00928	0.352
TRG_ENDOCYTIC_2	74	77	PF00928	0.227
TRG_ENDOCYTIC_2	84	87	PF00928	0.189
TRG_Pf-PMV_PEXEL_1	10	14	PF00026	0.386

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6R4	Leptomonas seymouri	80%	100%
A0A0N1I1Z1	Leptomonas seymouri	38%	100%
A0A0N1IA94	Leptomonas seymouri	25%	100%
A0A0N1PB60	Leptomonas seymouri	35%	100%
A0A0S4IJE0	Bodo saltans	37%	82%
A0A0S4JRZ8	Bodo saltans	37%	100%
A0A0S4KIJ1	Bodo saltans	57%	100%
A0A0S4KJ33	Bodo saltans	37%	100%
A0A1X0NPI5	Trypanosomatidae	61%	100%
A0A1X0PAA1	Trypanosomatidae	30%	78%
A0A3Q8II46	Leishmania donovani	34%	100%
A0A3S5H5C0	Leishmania donovani	34%	100%
A0A3S5H604	Leishmania donovani	100%	100%
A0A3S7WYK3	Leishmania donovani	39%	100%
A0A422MY24	Trypanosoma rangeli	62%	100%
A0A422NDI3	Trypanosoma rangeli	37%	86%
A0A422NWQ7	Trypanosoma rangeli	38%	100%
A0A451EJH5	Leishmania donovani	36%	100%
A4H402	Leishmania braziliensis	35%	100%
A4H533	Leishmania braziliensis	92%	99%
A4H861	Leishmania braziliensis	41%	76%
A4HDS4	Leishmania braziliensis	39%	100%
A4HLY8	Leishmania braziliensis	43%	100%
A4HRN6	Leishmania infantum	36%	100%
A4HS64	Leishmania infantum	34%	100%
A4I122	Leishmania infantum	39%	100%
A4IB24	Leishmania infantum	34%	100%
A6H795	Bos taurus	28%	100%
B5DEI4	Rattus norvegicus	28%	100%
C9ZJ18	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	92%
C9ZJA6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	73%
C9ZMR5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	100%
C9ZMW8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	83%
C9ZMZ2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
C9ZUL2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	60%	100%
E9ACB4	Leishmania major	36%	100%
E9AJK2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
E9AK51	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E9ALA2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
E9AX59	Leishmania mexicana (strain MHOM/GT/2001/U1103)	39%	100%
E9B4B7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	43%	76%
E9B609	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	100%
O00102	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	35%	100%
O60015	Pichia angusta	42%	97%
O74810	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	33%	84%
P0C8G3	African swine fever virus (isolate Pig/Kenya/KEN-50/1950)	34%	83%
P0C8G4	African swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996)	34%	84%
P0C8G5	African swine fever virus (isolate Warthog/Namibia/Wart80/1980)	33%	82%
P25868	Triticum aestivum	30%	100%
P25869	African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983)	35%	85%
P27949	African swine fever virus (strain Badajoz 1971 Vero-adapted)	34%	85%
P34477	Caenorhabditis elegans	34%	100%
P40984	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	35%	100%
P42747	Arabidopsis thaliana	31%	100%
P42749	Arabidopsis thaliana	35%	98%
P49427	Homo sapiens	33%	77%
P49428	Komagataella pastoris	36%	89%
P52478	Caenorhabditis elegans	39%	95%
P52484	Caenorhabditis elegans	32%	85%
P52485	Drosophila melanogaster	35%	78%
P60604	Homo sapiens	33%	100%
P60605	Mus musculus	33%	100%
Q02159	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	34%	100%
Q17QG5	Bos taurus	33%	100%
Q1JPX4	Danio rerio	27%	100%
Q28FC1	Xenopus tropicalis	28%	100%
Q29503	Oryctolagus cuniculus	32%	76%
Q4Q3Q8	Leishmania major	41%	75%
Q4QAB7	Leishmania major	39%	100%
Q4QIK2	Leishmania major	98%	100%
Q54I43	Dictyostelium discoideum	32%	85%
Q55EY8	Dictyostelium discoideum	31%	100%
Q5JXB2	Homo sapiens	32%	100%
Q5RF84	Pongo abelii	33%	100%
Q5UQC9	Acanthamoeba polyphaga mimivirus	28%	100%
Q6C093	Yarrowia lipolytica (strain CLIB 122 / E 150)	37%	100%
Q6FQK7	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	29%	74%
Q6ZWZ2	Mus musculus	32%	76%
Q712K3	Homo sapiens	32%	76%
Q74Z34	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	27%	75%
Q7ZY08	Xenopus laevis	29%	95%
Q8CFI2	Mus musculus	33%	77%
Q8LGF7	Arabidopsis thaliana	48%	100%
Q8VDW4	Mus musculus	28%	100%
Q94A97	Arabidopsis thaliana	32%	100%
Q95017	Caenorhabditis elegans	30%	100%
Q96B02	Homo sapiens	28%	100%
Q9CQ37	Mus musculus	30%	89%
Q9FF66	Arabidopsis thaliana	33%	73%
Q9FZ48	Arabidopsis thaliana	32%	100%
Q9GNC1	Leishmania major	34%	88%
Q9NED4	Leishmania major	34%	100%
Q9NPD8	Homo sapiens	28%	92%
Q9UTN8	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	33%	100%
V5BYE2	Trypanosoma cruzi	64%	100%