Pre-mRNA-splicing factor ISY1

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Isy1-like splicing family - putative

Species:

Leishmania infantum

UniProt:

A4HT23_LEIIN

TriTrypDb:

LINF_070005100

Length:

293

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	11
GO:0043226	organelle	2	11
GO:0043227	membrane-bounded organelle	3	11
GO:0043229	intracellular organelle	3	11
GO:0043231	intracellular membrane-bounded organelle	4	11
GO:0110165	cellular anatomical entity	1	11
GO:0000974	Prp19 complex	2	1
GO:0005681	spliceosomal complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0071013	catalytic step 2 spliceosome	3	1
GO:0071014	post-mRNA release spliceosomal complex	4	1
GO:0071020	post-spliceosomal complex	4	1
GO:0140513	nuclear protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1990904	ribonucleoprotein complex	2	1

Expansion

Sequence features

A4HT23

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HT23

Function

Biological processes
Term	Name	Level	Count
GO:0000350	generation of catalytic spliceosome for second transesterification step	7	12
GO:0009987	cellular process	1	12
GO:0016043	cellular component organization	3	12
GO:0022607	cellular component assembly	4	12
GO:0022618	ribonucleoprotein complex assembly	6	12
GO:0043933	protein-containing complex organization	4	12
GO:0065003	protein-containing complex assembly	5	12
GO:0071826	ribonucleoprotein complex subunit organization	5	12
GO:0071840	cellular component organization or biogenesis	2	12
GO:0000389	mRNA 3'-splice site recognition	8	1
GO:0006376	mRNA splice site selection	7	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	182	186	PF00656	0.559
CLV_NRD_NRD_1	127	129	PF00675	0.312
CLV_NRD_NRD_1	148	150	PF00675	0.292
CLV_NRD_NRD_1	166	168	PF00675	0.262
CLV_NRD_NRD_1	277	279	PF00675	0.294
CLV_NRD_NRD_1	41	43	PF00675	0.282
CLV_NRD_NRD_1	53	55	PF00675	0.319
CLV_PCSK_KEX2_1	168	170	PF00082	0.346
CLV_PCSK_KEX2_1	52	54	PF00082	0.291
CLV_PCSK_PC1ET2_1	168	170	PF00082	0.346
CLV_PCSK_PC1ET2_1	52	54	PF00082	0.291
CLV_PCSK_SKI1_1	150	154	PF00082	0.336
CLV_PCSK_SKI1_1	279	283	PF00082	0.309
CLV_PCSK_SKI1_1	79	83	PF00082	0.278
DEG_Nend_Nbox_1	1	3	PF02207	0.404
DOC_MAPK_gen_1	147	154	PF00069	0.562
DOC_PP1_RVXF_1	133	139	PF00149	0.563
DOC_PP1_RVXF_1	210	216	PF00149	0.556
DOC_PP1_RVXF_1	80	87	PF00149	0.493
DOC_USP7_MATH_1	161	165	PF00917	0.521
DOC_USP7_MATH_1	184	188	PF00917	0.563
DOC_USP7_MATH_1	20	24	PF00917	0.420
DOC_USP7_UBL2_3	235	239	PF12436	0.556
LIG_14-3-3_CanoR_1	135	139	PF00244	0.538
LIG_14-3-3_CanoR_1	246	256	PF00244	0.577
LIG_14-3-3_CterR_2	290	293	PF00244	0.590
LIG_Clathr_ClatBox_1	104	108	PF01394	0.501
LIG_Clathr_ClatBox_1	225	229	PF01394	0.525
LIG_FHA_1	10	16	PF00498	0.427
LIG_FHA_1	94	100	PF00498	0.559
LIG_HCF-1_HBM_1	206	209	PF13415	0.525
LIG_PCNA_yPIPBox_3	116	126	PF02747	0.487
LIG_SH2_STAT5	159	162	PF00017	0.445
LIG_SH2_STAT5	209	212	PF00017	0.512
LIG_SH2_STAT5	256	259	PF00017	0.501
LIG_SH2_STAT5	83	86	PF00017	0.517
LIG_SH3_3	191	197	PF00018	0.546
LIG_SH3_3	259	265	PF00018	0.470
LIG_SH3_3	66	72	PF00018	0.487
LIG_SUMO_SIM_par_1	103	108	PF11976	0.518
LIG_TRAF2_1	140	143	PF00917	0.486
LIG_TRAF2_1	174	177	PF00917	0.534
LIG_TRAF2_1	186	189	PF00917	0.536
LIG_UBA3_1	46	52	PF00899	0.501
LIG_UBA3_1	85	89	PF00899	0.517
MOD_CK2_1	171	177	PF00069	0.537
MOD_CK2_1	183	189	PF00069	0.500
MOD_GlcNHglycan	218	221	PF01048	0.349
MOD_GlcNHglycan	22	25	PF01048	0.522
MOD_GlcNHglycan	249	252	PF01048	0.322
MOD_GSK3_1	242	249	PF00069	0.551
MOD_GSK3_1	32	39	PF00069	0.519
MOD_NEK2_1	216	221	PF00069	0.581
MOD_NEK2_1	252	257	PF00069	0.556
MOD_NEK2_1	3	8	PF00069	0.399
MOD_PIKK_1	9	15	PF00454	0.429
MOD_PK_1	103	109	PF00069	0.558
MOD_PKA_1	42	48	PF00069	0.487
MOD_PKA_2	134	140	PF00069	0.581
MOD_PKB_1	169	177	PF00069	0.428
MOD_Plk_1	161	167	PF00069	0.589
MOD_Plk_4	11	17	PF00069	0.494
MOD_Plk_4	134	140	PF00069	0.565
MOD_Plk_4	42	48	PF00069	0.476
MOD_SUMO_for_1	88	91	PF00179	0.537
MOD_SUMO_rev_2	229	236	PF00179	0.512
MOD_SUMO_rev_2	255	263	PF00179	0.581
MOD_SUMO_rev_2	73	81	PF00179	0.519
TRG_DiLeu_BaEn_1	121	126	PF01217	0.487
TRG_DiLeu_BaEn_1	188	193	PF01217	0.525
TRG_ENDOCYTIC_2	83	86	PF00928	0.501
TRG_ER_diArg_1	146	149	PF00400	0.563
TRG_NLS_Bipartite_1	149	171	PF00514	0.561
TRG_NLS_MonoExtC_3	166	171	PF00514	0.561
TRG_NLS_MonoExtN_4	146	153	PF00514	0.581
TRG_NLS_MonoExtN_4	167	172	PF00514	0.509
TRG_Pf-PMV_PEXEL_1	158	162	PF00026	0.287
TRG_Pf-PMV_PEXEL_1	280	284	PF00026	0.328

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PAF4	Leptomonas seymouri	63%	100%
A0A0S4IZ16	Bodo saltans	36%	100%
A0A1X0NL28	Trypanosomatidae	43%	100%
A0A3R7KL93	Trypanosoma rangeli	43%	100%
A0A3S5H5V5	Leishmania donovani	99%	100%
A4H4U5	Leishmania braziliensis	80%	100%
C9ZUW9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	100%
E9AL11	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
Q4QIU3	Leishmania major	89%	100%
V5B8Q7	Trypanosoma cruzi	42%	100%