Putative ubiquitin-conjugating enzyme

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme E2 - putative

Species:

Leishmania infantum

UniProt:

A4HSL5_LEIIN

TriTrypDb:

LINF_050014500

Length:

465

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0016020	membrane	2	3
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	3

Expansion

Sequence features

A4HSL5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HSL5

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0016567	protein ubiquitination	7	1
GO:0019538	protein metabolic process	3	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044238	primary metabolic process	2	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	4
GO:0016874	ligase activity	2	4
GO:0004842	ubiquitin-protein transferase activity	4	1
GO:0016740	transferase activity	2	1
GO:0019787	ubiquitin-like protein transferase activity	3	1
GO:0061631	ubiquitin conjugating enzyme activity	5	1
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	1
GO:0140096	catalytic activity, acting on a protein	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	208	212	PF00656	0.704
CLV_C14_Caspase3-7	272	276	PF00656	0.675
CLV_NRD_NRD_1	16	18	PF00675	0.278
CLV_NRD_NRD_1	456	458	PF00675	0.312
CLV_PCSK_KEX2_1	16	18	PF00082	0.275
CLV_PCSK_KEX2_1	456	458	PF00082	0.312
CLV_PCSK_KEX2_1	9	11	PF00082	0.275
CLV_PCSK_PC1ET2_1	9	11	PF00082	0.275
CLV_PCSK_SKI1_1	300	304	PF00082	0.469
CLV_PCSK_SKI1_1	422	426	PF00082	0.462
DEG_APCC_DBOX_1	455	463	PF00400	0.388
DEG_SCF_FBW7_2	364	371	PF00400	0.652
DOC_CKS1_1	290	295	PF01111	0.712
DOC_CYCLIN_yCln2_LP_2	189	195	PF00134	0.677
DOC_MAPK_DCC_7	180	189	PF00069	0.557
DOC_MAPK_MEF2A_6	180	189	PF00069	0.557
DOC_MAPK_RevD_3	443	457	PF00069	0.385
DOC_PP1_RVXF_1	420	426	PF00149	0.630
DOC_PP2B_LxvP_1	187	190	PF13499	0.674
DOC_PP2B_LxvP_1	73	76	PF13499	0.475
DOC_SPAK_OSR1_1	86	90	PF12202	0.529
DOC_USP7_MATH_1	195	199	PF00917	0.691
DOC_USP7_MATH_1	225	229	PF00917	0.705
DOC_USP7_MATH_1	238	242	PF00917	0.750
DOC_USP7_MATH_1	268	272	PF00917	0.708
DOC_USP7_MATH_1	385	389	PF00917	0.661
DOC_USP7_MATH_1	400	404	PF00917	0.596
DOC_WW_Pin1_4	174	179	PF00397	0.649
DOC_WW_Pin1_4	215	220	PF00397	0.746
DOC_WW_Pin1_4	223	228	PF00397	0.688
DOC_WW_Pin1_4	229	234	PF00397	0.675
DOC_WW_Pin1_4	260	265	PF00397	0.712
DOC_WW_Pin1_4	278	283	PF00397	0.728
DOC_WW_Pin1_4	289	294	PF00397	0.671
DOC_WW_Pin1_4	325	330	PF00397	0.737
DOC_WW_Pin1_4	364	369	PF00397	0.645
DOC_WW_Pin1_4	380	385	PF00397	0.650
DOC_WW_Pin1_4	402	407	PF00397	0.625
LIG_Actin_WH2_2	443	458	PF00022	0.380
LIG_APCC_ABBA_1	170	175	PF00400	0.576
LIG_BIR_II_1	1	5	PF00653	0.553
LIG_BIR_III_2	381	385	PF00653	0.669
LIG_BRCT_BRCA1_1	265	269	PF00533	0.651
LIG_BRCT_BRCA1_1	445	449	PF00533	0.286
LIG_BRCT_BRCA1_1	83	87	PF00533	0.529
LIG_deltaCOP1_diTrp_1	144	153	PF00928	0.582
LIG_deltaCOP1_diTrp_1	39	42	PF00928	0.475
LIG_EH1_1	448	456	PF00400	0.279
LIG_EVH1_1	187	191	PF00568	0.755
LIG_FHA_1	106	112	PF00498	0.475
LIG_FHA_1	140	146	PF00498	0.568
LIG_FHA_1	160	166	PF00498	0.583
LIG_FHA_1	281	287	PF00498	0.745
LIG_FHA_1	381	387	PF00498	0.713
LIG_FHA_2	174	180	PF00498	0.607
LIG_FHA_2	270	276	PF00498	0.673
LIG_GBD_Chelix_1	444	452	PF00786	0.331
LIG_LIR_Apic_2	27	32	PF02991	0.529
LIG_LIR_Gen_1	322	332	PF02991	0.711
LIG_LIR_Gen_1	446	455	PF02991	0.279
LIG_LIR_Nem_3	12	18	PF02991	0.475
LIG_LIR_Nem_3	446	452	PF02991	0.286
LIG_NRBOX	458	464	PF00104	0.431
LIG_Pex14_1	105	109	PF04695	0.475
LIG_Pex14_2	38	42	PF04695	0.475
LIG_PROFILIN_1	341	347	PF00235	0.611
LIG_REV1ctd_RIR_1	78	87	PF16727	0.475
LIG_SH2_CRK	15	19	PF00017	0.460
LIG_SH2_PTP2	29	32	PF00017	0.495
LIG_SH2_SRC	109	112	PF00017	0.547
LIG_SH2_STAP1	109	113	PF00017	0.475
LIG_SH2_STAP1	3	7	PF00017	0.521
LIG_SH2_STAP1	356	360	PF00017	0.628
LIG_SH2_STAT5	29	32	PF00017	0.495
LIG_SH3_1	261	267	PF00018	0.690
LIG_SH3_1	69	75	PF00018	0.475
LIG_SH3_3	182	188	PF00018	0.692
LIG_SH3_3	189	195	PF00018	0.699
LIG_SH3_3	216	222	PF00018	0.709
LIG_SH3_3	248	254	PF00018	0.695
LIG_SH3_3	261	267	PF00018	0.657
LIG_SH3_3	330	336	PF00018	0.684
LIG_SH3_3	338	344	PF00018	0.710
LIG_SH3_3	411	417	PF00018	0.659
LIG_SH3_3	69	75	PF00018	0.475
LIG_SUMO_SIM_par_1	136	142	PF11976	0.557
LIG_TRAF2_1	32	35	PF00917	0.426
LIG_TRFH_1	71	75	PF08558	0.312
LIG_UBA3_1	137	143	PF00899	0.363
LIG_WRC_WIRS_1	444	449	PF05994	0.340
MOD_CDC14_SPxK_1	177	180	PF00782	0.555
MOD_CDC14_SPxK_1	328	331	PF00782	0.641
MOD_CDK_SPK_2	364	369	PF00069	0.489
MOD_CDK_SPxK_1	174	180	PF00069	0.545
MOD_CDK_SPxK_1	325	331	PF00069	0.648
MOD_CK1_1	2	8	PF00069	0.415
MOD_CK1_1	226	232	PF00069	0.676
MOD_CK1_1	304	310	PF00069	0.649
MOD_CK1_1	322	328	PF00069	0.474
MOD_CK1_1	405	411	PF00069	0.558
MOD_CK1_1	95	101	PF00069	0.330
MOD_CK2_1	173	179	PF00069	0.397
MOD_CK2_1	304	310	PF00069	0.675
MOD_GlcNHglycan	197	200	PF01048	0.626
MOD_GlcNHglycan	223	226	PF01048	0.705
MOD_GlcNHglycan	303	306	PF01048	0.738
MOD_GlcNHglycan	387	390	PF01048	0.597
MOD_GlcNHglycan	402	405	PF01048	0.464
MOD_GlcNHglycan	5	8	PF01048	0.389
MOD_GlcNHglycan	83	86	PF01048	0.452
MOD_GSK3_1	147	154	PF00069	0.386
MOD_GSK3_1	221	228	PF00069	0.683
MOD_GSK3_1	274	281	PF00069	0.583
MOD_GSK3_1	380	387	PF00069	0.597
MOD_GSK3_1	402	409	PF00069	0.494
MOD_GSK3_1	92	99	PF00069	0.324
MOD_NEK2_1	113	118	PF00069	0.312
MOD_NEK2_1	153	158	PF00069	0.481
MOD_NEK2_1	269	274	PF00069	0.590
MOD_NEK2_1	430	435	PF00069	0.644
MOD_NEK2_1	65	70	PF00069	0.328
MOD_NEK2_1	79	84	PF00069	0.371
MOD_NEK2_2	40	45	PF00069	0.312
MOD_PIKK_1	165	171	PF00454	0.423
MOD_PKA_1	9	15	PF00069	0.312
MOD_PKA_2	9	15	PF00069	0.329
MOD_Plk_1	274	280	PF00069	0.545
MOD_Plk_1	430	436	PF00069	0.535
MOD_Plk_1	50	56	PF00069	0.331
MOD_Plk_4	133	139	PF00069	0.353
MOD_Plk_4	359	365	PF00069	0.558
MOD_Plk_4	443	449	PF00069	0.388
MOD_ProDKin_1	174	180	PF00069	0.552
MOD_ProDKin_1	215	221	PF00069	0.688
MOD_ProDKin_1	223	229	PF00069	0.609
MOD_ProDKin_1	231	237	PF00069	0.571
MOD_ProDKin_1	260	266	PF00069	0.641
MOD_ProDKin_1	278	284	PF00069	0.667
MOD_ProDKin_1	289	295	PF00069	0.584
MOD_ProDKin_1	325	331	PF00069	0.681
MOD_ProDKin_1	364	370	PF00069	0.553
MOD_ProDKin_1	380	386	PF00069	0.557
MOD_ProDKin_1	402	408	PF00069	0.522
MOD_SUMO_for_1	201	204	PF00179	0.621
MOD_SUMO_for_1	308	311	PF00179	0.590
MOD_SUMO_rev_2	136	145	PF00179	0.335
TRG_DiLeu_BaLyEn_6	434	439	PF01217	0.429
TRG_ENDOCYTIC_2	15	18	PF00928	0.312
TRG_ER_diArg_1	15	17	PF00400	0.315
TRG_ER_diArg_1	455	457	PF00400	0.312

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1C5	Leptomonas seymouri	45%	96%
A0A3S5H5K9	Leishmania donovani	97%	100%
A4H4D6	Leishmania braziliensis	59%	94%
E9AKJ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	68%	93%
Q4QJA9	Leishmania major	81%	99%