LeishMANIAdb
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tRNA 4-demethylwyosine synthase (AdoMet-dependent)

Quick info Annotations Function or PPIs Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:
tRNA 4-demethylwyosine synthase (AdoMet-dependent)
Gene product:
Flavodoxin/Radical SAM superfamily/Wyosine base formation - putative
Species:
Leishmania infantum
UniProt:
A4HSJ7_LEIIN
TriTrypDb:
LINF_050012700
Length:
824

Annotations

Annotations by Jardim et al.

Metal Binding, Flavodoxin/Radical SAM superfamily

Localization

Secreted promastigote
Source Evidence on protein Close homologs
Cuervo et al. no yes: 0
Hassani et al. no yes: 0
Forrest at al. (metacyclic) no yes: 0
Forrest at al. (procyclic) no yes: 0
Silverman et al. no yes: 0
Pissara et al. no yes: 0
Secreted amastigote
Source Evidence on protein Close homologs
Pires et al. no yes: 0
Exosome
Source Evidence on protein Close homologs
Silverman et al. no yes: 0
Glycosome
Source Evidence on protein Close homologs
Jamdhade et al. no yes: 0
Predictions
Source Evidence on protein Close homologs
DeepLoc
SignalP6 no yes: 0, no: 9
NetGPI no yes: 0, no: 9
Cellular components
Term Name Level Count
GO:0005737 cytoplasm 2 10
GO:0110165 cellular anatomical entity 1 10
GO:0016020 membrane 2 3

Expansion

Sequence features

A4HSJ7
Sequence
MSA
Disorder
Secondary
Topology
Domains
SignalP
GPI
Phosphorylations
ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HSJ7

Function

Biological processes
Term Name Level Count
GO:0006139 nucleobase-containing compound metabolic process 3 10
GO:0006396 RNA processing 6 10
GO:0006399 tRNA metabolic process 7 10
GO:0006725 cellular aromatic compound metabolic process 3 10
GO:0006807 nitrogen compound metabolic process 2 10
GO:0008033 tRNA processing 8 10
GO:0008152 metabolic process 1 10
GO:0009987 cellular process 1 10
GO:0016070 RNA metabolic process 5 10
GO:0034470 ncRNA processing 7 10
GO:0034641 cellular nitrogen compound metabolic process 3 10
GO:0034660 ncRNA metabolic process 6 10
GO:0043170 macromolecule metabolic process 3 10
GO:0044237 cellular metabolic process 2 10
GO:0044238 primary metabolic process 2 10
GO:0046483 heterocycle metabolic process 3 10
GO:0071704 organic substance metabolic process 2 10
GO:0090304 nucleic acid metabolic process 4 10
GO:1901360 organic cyclic compound metabolic process 3 10
GO:0006400 tRNA modification 6 1
GO:0009058 biosynthetic process 2 1
GO:0009451 RNA modification 5 1
GO:0031590 wybutosine metabolic process 4 1
GO:0031591 wybutosine biosynthetic process 5 1
GO:0043412 macromolecule modification 4 1
GO:1901135 carbohydrate derivative metabolic process 3 1
GO:1901137 carbohydrate derivative biosynthetic process 4 1
GO:1901564 organonitrogen compound metabolic process 3 1
GO:1901566 organonitrogen compound biosynthetic process 4 1
GO:1901576 organic substance biosynthetic process 3 1
GO:1901657 glycosyl compound metabolic process 4 1
GO:1901659 glycosyl compound biosynthetic process 5 1
Molecular functions
Term Name Level Count
GO:0000166 nucleotide binding 3 10
GO:0003824 catalytic activity 1 10
GO:0005488 binding 1 10
GO:0010181 FMN binding 4 10
GO:0016829 lyase activity 2 10
GO:0032553 ribonucleotide binding 3 10
GO:0036094 small molecule binding 2 10
GO:0043167 ion binding 2 10
GO:0043168 anion binding 3 10
GO:0043169 cation binding 3 10
GO:0046872 metal ion binding 4 10
GO:0051536 iron-sulfur cluster binding 3 10
GO:0051539 4 iron, 4 sulfur cluster binding 4 10
GO:0051540 metal cluster binding 2 10
GO:0097159 organic cyclic compound binding 2 10
GO:0097367 carbohydrate derivative binding 2 10
GO:1901265 nucleoside phosphate binding 3 10
GO:1901363 heterocyclic compound binding 2 10

Putative motif mimicry

Leishmania From To Domain/Motif Score
CLV_C14_Caspase3-7 175 179 PF00656 0.516
CLV_C14_Caspase3-7 366 370 PF00656 0.791
CLV_NRD_NRD_1 207 209 PF00675 0.391
CLV_NRD_NRD_1 361 363 PF00675 0.559
CLV_NRD_NRD_1 627 629 PF00675 0.249
CLV_NRD_NRD_1 811 813 PF00675 0.463
CLV_PCSK_KEX2_1 209 211 PF00082 0.357
CLV_PCSK_KEX2_1 605 607 PF00082 0.308
CLV_PCSK_PC1ET2_1 209 211 PF00082 0.357
CLV_PCSK_PC1ET2_1 605 607 PF00082 0.308
CLV_PCSK_SKI1_1 193 197 PF00082 0.313
CLV_PCSK_SKI1_1 448 452 PF00082 0.327
CLV_PCSK_SKI1_1 482 486 PF00082 0.290
CLV_PCSK_SKI1_1 584 588 PF00082 0.293
CLV_PCSK_SKI1_1 609 613 PF00082 0.279
CLV_PCSK_SKI1_1 632 636 PF00082 0.282
CLV_PCSK_SKI1_1 666 670 PF00082 0.290
CLV_PCSK_SKI1_1 754 758 PF00082 0.328
DEG_APCC_DBOX_1 53 61 PF00400 0.652
DEG_MDM2_SWIB_1 612 620 PF02201 0.490
DEG_MDM2_SWIB_1 757 765 PF02201 0.587
DEG_Nend_Nbox_1 1 3 PF02207 0.443
DOC_ANK_TNKS_1 349 356 PF00023 0.766
DOC_CKS1_1 709 714 PF01111 0.649
DOC_CYCLIN_yCln2_LP_2 12 18 PF00134 0.408
DOC_MAPK_DCC_7 499 509 PF00069 0.531
DOC_MAPK_gen_1 584 594 PF00069 0.487
DOC_MAPK_gen_1 628 639 PF00069 0.493
DOC_MAPK_MEF2A_6 501 509 PF00069 0.531
DOC_MAPK_MEF2A_6 584 592 PF00069 0.498
DOC_MAPK_MEF2A_6 79 88 PF00069 0.707
DOC_PP2B_LxvP_1 12 15 PF13499 0.408
DOC_PP2B_LxvP_1 240 243 PF13499 0.561
DOC_PP4_FxxP_1 245 248 PF00568 0.537
DOC_SPAK_OSR1_1 210 214 PF12202 0.524
DOC_USP7_MATH_1 102 106 PF00917 0.549
DOC_USP7_MATH_1 297 301 PF00917 0.775
DOC_USP7_MATH_1 317 321 PF00917 0.545
DOC_WW_Pin1_4 124 129 PF00397 0.508
DOC_WW_Pin1_4 292 297 PF00397 0.750
DOC_WW_Pin1_4 471 476 PF00397 0.510
DOC_WW_Pin1_4 527 532 PF00397 0.479
DOC_WW_Pin1_4 597 602 PF00397 0.477
DOC_WW_Pin1_4 708 713 PF00397 0.656
DOC_WW_Pin1_4 71 76 PF00397 0.760
DOC_WW_Pin1_4 78 83 PF00397 0.756
DOC_WW_Pin1_4 787 792 PF00397 0.526
LIG_14-3-3_CanoR_1 203 207 PF00244 0.561
LIG_14-3-3_CanoR_1 262 272 PF00244 0.573
LIG_14-3-3_CanoR_1 274 278 PF00244 0.491
LIG_14-3-3_CanoR_1 445 451 PF00244 0.608
LIG_14-3-3_CanoR_1 568 573 PF00244 0.553
LIG_14-3-3_CanoR_1 618 626 PF00244 0.551
LIG_14-3-3_CanoR_1 636 640 PF00244 0.388
LIG_14-3-3_CanoR_1 816 821 PF00244 0.715
LIG_Actin_WH2_2 256 272 PF00022 0.604
LIG_Actin_WH2_2 589 607 PF00022 0.508
LIG_BRCT_BRCA1_1 184 188 PF00533 0.490
LIG_BRCT_BRCA1_1 494 498 PF00533 0.508
LIG_Clathr_ClatBox_1 749 753 PF01394 0.572
LIG_deltaCOP1_diTrp_1 758 764 PF00928 0.587
LIG_FHA_1 1 7 PF00498 0.355
LIG_FHA_1 118 124 PF00498 0.630
LIG_FHA_1 175 181 PF00498 0.547
LIG_FHA_1 255 261 PF00498 0.574
LIG_FHA_1 264 270 PF00498 0.507
LIG_FHA_1 280 286 PF00498 0.549
LIG_FHA_1 317 323 PF00498 0.724
LIG_FHA_1 472 478 PF00498 0.490
LIG_FHA_1 489 495 PF00498 0.490
LIG_FHA_1 643 649 PF00498 0.490
LIG_FHA_1 806 812 PF00498 0.697
LIG_FHA_2 157 163 PF00498 0.558
LIG_FHA_2 173 179 PF00498 0.406
LIG_FHA_2 364 370 PF00498 0.745
LIG_FHA_2 598 604 PF00498 0.490
LIG_FHA_2 761 767 PF00498 0.525
LIG_FHA_2 780 786 PF00498 0.448
LIG_GBD_Chelix_1 17 25 PF00786 0.444
LIG_LIR_Gen_1 185 196 PF02991 0.490
LIG_LIR_Gen_1 223 233 PF02991 0.509
LIG_LIR_Gen_1 661 670 PF02991 0.503
LIG_LIR_Gen_1 766 774 PF02991 0.533
LIG_LIR_Gen_1 779 789 PF02991 0.543
LIG_LIR_Nem_3 223 229 PF02991 0.509
LIG_LIR_Nem_3 259 264 PF02991 0.560
LIG_LIR_Nem_3 417 423 PF02991 0.572
LIG_LIR_Nem_3 461 465 PF02991 0.515
LIG_LIR_Nem_3 661 665 PF02991 0.503
LIG_LIR_Nem_3 763 767 PF02991 0.511
LIG_LIR_Nem_3 768 774 PF02991 0.533
LIG_LIR_Nem_3 779 784 PF02991 0.553
LIG_LIR_Nem_3 785 789 PF02991 0.539
LIG_LIR_Nem_3 790 796 PF02991 0.495
LIG_LYPXL_S_1 419 423 PF13949 0.374
LIG_LYPXL_S_1 553 557 PF13949 0.290
LIG_LYPXL_yS_3 420 423 PF13949 0.580
LIG_LYPXL_yS_3 554 557 PF13949 0.490
LIG_MAD2 809 817 PF02301 0.710
LIG_Pex14_2 612 616 PF04695 0.490
LIG_Pex14_2 757 761 PF04695 0.598
LIG_PTB_Apo_2 573 580 PF02174 0.490
LIG_SH2_CRK 166 170 PF00017 0.475
LIG_SH2_CRK 432 436 PF00017 0.540
LIG_SH2_CRK 517 521 PF00017 0.561
LIG_SH2_CRK 709 713 PF00017 0.664
LIG_SH2_CRK 786 790 PF00017 0.533
LIG_SH2_NCK_1 771 775 PF00017 0.577
LIG_SH2_NCK_1 786 790 PF00017 0.493
LIG_SH2_STAP1 166 170 PF00017 0.531
LIG_SH2_STAP1 22 26 PF00017 0.637
LIG_SH2_STAP1 251 255 PF00017 0.599
LIG_SH2_STAP1 651 655 PF00017 0.490
LIG_SH2_STAP1 767 771 PF00017 0.595
LIG_SH2_STAT3 101 104 PF00017 0.529
LIG_SH2_STAT5 176 179 PF00017 0.480
LIG_SH2_STAT5 593 596 PF00017 0.490
LIG_SH2_STAT5 670 673 PF00017 0.490
LIG_SH3_3 131 137 PF00018 0.625
LIG_SH3_3 177 183 PF00018 0.591
LIG_SH3_3 243 249 PF00018 0.541
LIG_SH3_3 545 551 PF00018 0.490
LIG_SH3_3 83 89 PF00018 0.779
LIG_SH3_4 587 594 PF00018 0.469
LIG_SUMO_SIM_anti_2 723 728 PF11976 0.677
LIG_SUMO_SIM_par_1 748 753 PF11976 0.571
LIG_TRAF2_1 600 603 PF00917 0.508
LIG_TRAF2_1 728 731 PF00917 0.669
LIG_TYR_ITIM 20 25 PF00017 0.565
LIG_TYR_ITIM 418 423 PF00017 0.569
LIG_TYR_ITIM 552 557 PF00017 0.490
LIG_TYR_ITIM 784 789 PF00017 0.613
LIG_UBA3_1 749 754 PF00899 0.578
LIG_WRC_WIRS_1 1 6 PF05994 0.452
LIG_WRC_WIRS_1 569 574 PF05994 0.512
LIG_WW_1 248 251 PF00397 0.545
LIG_WW_3 181 185 PF00397 0.561
MOD_CDK_SPxK_1 527 533 PF00069 0.490
MOD_CK1_1 105 111 PF00069 0.561
MOD_CK1_1 117 123 PF00069 0.509
MOD_CK1_1 174 180 PF00069 0.485
MOD_CK1_1 273 279 PF00069 0.615
MOD_CK1_1 28 34 PF00069 0.706
MOD_CK1_1 320 326 PF00069 0.732
MOD_CK1_1 674 680 PF00069 0.490
MOD_CK1_1 776 782 PF00069 0.572
MOD_CK2_1 156 162 PF00069 0.561
MOD_CK2_1 297 303 PF00069 0.764
MOD_CK2_1 380 386 PF00069 0.772
MOD_CK2_1 597 603 PF00069 0.490
MOD_CMANNOS 761 764 PF00535 0.302
MOD_Cter_Amidation 206 209 PF01082 0.336
MOD_GlcNHglycan 299 302 PF01048 0.591
MOD_GlcNHglycan 310 313 PF01048 0.527
MOD_GlcNHglycan 322 325 PF01048 0.520
MOD_GlcNHglycan 438 441 PF01048 0.311
MOD_GlcNHglycan 63 66 PF01048 0.531
MOD_GlcNHglycan 67 70 PF01048 0.519
MOD_GlcNHglycan 775 778 PF01048 0.358
MOD_GSK3_1 250 257 PF00069 0.571
MOD_GSK3_1 299 306 PF00069 0.758
MOD_GSK3_1 313 320 PF00069 0.620
MOD_GSK3_1 406 413 PF00069 0.573
MOD_GSK3_1 488 495 PF00069 0.508
MOD_GSK3_1 568 575 PF00069 0.489
MOD_GSK3_1 61 68 PF00069 0.735
MOD_GSK3_1 666 673 PF00069 0.490
MOD_GSK3_1 776 783 PF00069 0.615
MOD_GSK3_1 816 823 PF00069 0.678
MOD_N-GLC_1 317 322 PF02516 0.563
MOD_N-GLC_1 465 470 PF02516 0.388
MOD_N-GLC_1 675 680 PF02516 0.238
MOD_N-GLC_2 153 155 PF02516 0.361
MOD_N-GLC_2 481 483 PF02516 0.290
MOD_NEK2_1 171 176 PF00069 0.508
MOD_NEK2_1 20 25 PF00069 0.453
MOD_NEK2_1 291 296 PF00069 0.754
MOD_NEK2_1 346 351 PF00069 0.755
MOD_NEK2_1 434 439 PF00069 0.519
MOD_NEK2_1 572 577 PF00069 0.490
MOD_NEK2_1 635 640 PF00069 0.490
MOD_NEK2_1 773 778 PF00069 0.556
MOD_NEK2_1 8 13 PF00069 0.389
MOD_NEK2_2 281 286 PF00069 0.658
MOD_NEK2_2 458 463 PF00069 0.514
MOD_OFUCOSY 740 746 PF10250 0.320
MOD_PIKK_1 100 106 PF00454 0.538
MOD_PIKK_1 182 188 PF00454 0.508
MOD_PIKK_1 254 260 PF00454 0.651
MOD_PIKK_1 340 346 PF00454 0.746
MOD_PIKK_1 465 471 PF00454 0.587
MOD_PIKK_1 47 53 PF00454 0.672
MOD_PKA_1 286 292 PF00069 0.692
MOD_PKA_2 202 208 PF00069 0.531
MOD_PKA_2 273 279 PF00069 0.584
MOD_PKA_2 444 450 PF00069 0.542
MOD_PKA_2 567 573 PF00069 0.536
MOD_PKA_2 635 641 PF00069 0.490
MOD_PKA_2 815 821 PF00069 0.686
MOD_Plk_1 317 323 PF00069 0.733
MOD_Plk_1 675 681 PF00069 0.438
MOD_Plk_1 779 785 PF00069 0.623
MOD_Plk_1 805 811 PF00069 0.645
MOD_Plk_2-3 780 786 PF00069 0.612
MOD_Plk_4 156 162 PF00069 0.545
MOD_Plk_4 256 262 PF00069 0.582
MOD_Plk_4 317 323 PF00069 0.780
MOD_Plk_4 446 452 PF00069 0.547
MOD_Plk_4 458 464 PF00069 0.470
MOD_Plk_4 543 549 PF00069 0.490
MOD_Plk_4 568 574 PF00069 0.512
MOD_Plk_4 635 641 PF00069 0.490
MOD_Plk_4 760 766 PF00069 0.511
MOD_ProDKin_1 124 130 PF00069 0.512
MOD_ProDKin_1 292 298 PF00069 0.751
MOD_ProDKin_1 471 477 PF00069 0.490
MOD_ProDKin_1 527 533 PF00069 0.479
MOD_ProDKin_1 597 603 PF00069 0.477
MOD_ProDKin_1 708 714 PF00069 0.652
MOD_ProDKin_1 71 77 PF00069 0.758
MOD_ProDKin_1 78 84 PF00069 0.754
MOD_ProDKin_1 787 793 PF00069 0.539
MOD_SUMO_rev_2 597 607 PF00179 0.485
MOD_SUMO_rev_2 661 668 PF00179 0.490
TRG_DiLeu_BaEn_1 414 419 PF01217 0.593
TRG_ENDOCYTIC_2 166 169 PF00928 0.475
TRG_ENDOCYTIC_2 22 25 PF00928 0.681
TRG_ENDOCYTIC_2 420 423 PF00928 0.580
TRG_ENDOCYTIC_2 432 435 PF00928 0.540
TRG_ENDOCYTIC_2 517 520 PF00928 0.561
TRG_ENDOCYTIC_2 554 557 PF00928 0.490
TRG_ENDOCYTIC_2 593 596 PF00928 0.490
TRG_ENDOCYTIC_2 651 654 PF00928 0.490
TRG_ENDOCYTIC_2 767 770 PF00928 0.527
TRG_ENDOCYTIC_2 771 774 PF00928 0.533
TRG_ENDOCYTIC_2 786 789 PF00928 0.538
TRG_ER_diArg_1 208 211 PF00400 0.563
TRG_ER_diArg_1 451 454 PF00400 0.525
TRG_ER_diArg_1 732 735 PF00400 0.641
TRG_NES_CRM1_1 133 148 PF08389 0.504
TRG_Pf-PMV_PEXEL_1 231 235 PF00026 0.361
TRG_Pf-PMV_PEXEL_1 609 613 PF00026 0.290

Homologs

Protein Taxonomy Sequence identity Coverage
A0A0N1I710 Leptomonas seymouri 66% 93%
A0A3S5H5J8 Leishmania donovani 100% 100%
A0A3S5IRA8 Trypanosoma rangeli 55% 100%
A4H4B8 Leishmania braziliensis 81% 98%
C9ZU99 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 54% 99%
E9AKI1 Leishmania mexicana (strain MHOM/GT/2001/U1103) 89% 97%
Q4QJC7 Leishmania major 95% 100%
V5AVD9 Trypanosoma cruzi 54% 100%

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LeishMANIAdb - Server version: v0.0.2. - Database version: v0.0.1. - ChangeLog - © 2022-2024 Protein Bioinformatics Research Group, Institute of Enzymology, RCNS