Probable eukaryotic initiation factor 4A

Quick info Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ATP-dependent RNA helicase - putative

Species:

Leishmania infantum

UniProt:

A4HSF8_LEIIN

TriTrypDb:

LINF_050008600

Length:

625

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005730	nucleolus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A4HSF8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HSF8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006364	rRNA processing	8	1
GO:0006396	RNA processing	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0016072	rRNA metabolic process	7	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003676	nucleic acid binding	3	12
GO:0003724	RNA helicase activity	3	12
GO:0003743	translation initiation factor activity	4	12
GO:0003824	catalytic activity	1	12
GO:0004386	helicase activity	2	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0008135	translation factor activity, RNA binding	3	12
GO:0008186	ATP-dependent activity, acting on RNA	2	12
GO:0016787	hydrolase activity	2	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0045182	translation regulator activity	1	12
GO:0090079	translation regulator activity, nucleic acid binding	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:0140657	ATP-dependent activity	1	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	112	116	PF00656	0.470
CLV_NRD_NRD_1	505	507	PF00675	0.554
CLV_NRD_NRD_1	558	560	PF00675	0.626
CLV_NRD_NRD_1	595	597	PF00675	0.654
CLV_NRD_NRD_1	617	619	PF00675	0.728
CLV_PCSK_FUR_1	556	560	PF00082	0.691
CLV_PCSK_KEX2_1	555	557	PF00082	0.731
CLV_PCSK_KEX2_1	558	560	PF00082	0.680
CLV_PCSK_KEX2_1	595	597	PF00082	0.688
CLV_PCSK_KEX2_1	619	621	PF00082	0.710
CLV_PCSK_PC1ET2_1	555	557	PF00082	0.693
CLV_PCSK_PC1ET2_1	619	621	PF00082	0.710
CLV_PCSK_SKI1_1	100	104	PF00082	0.249
CLV_PCSK_SKI1_1	132	136	PF00082	0.233
CLV_PCSK_SKI1_1	143	147	PF00082	0.212
CLV_PCSK_SKI1_1	182	186	PF00082	0.206
CLV_PCSK_SKI1_1	507	511	PF00082	0.472
CLV_PCSK_SKI1_1	576	580	PF00082	0.580
CLV_PCSK_SKI1_1	596	600	PF00082	0.417
CLV_PCSK_SKI1_1	602	606	PF00082	0.588
CLV_PCSK_SKI1_1	619	623	PF00082	0.528
CLV_Separin_Metazoa	349	353	PF03568	0.415
DEG_APCC_DBOX_1	346	354	PF00400	0.429
DEG_SCF_FBW7_2	74	81	PF00400	0.415
DOC_CDC14_PxL_1	319	327	PF14671	0.385
DOC_CKS1_1	22	27	PF01111	0.472
DOC_CKS1_1	75	80	PF01111	0.415
DOC_CYCLIN_RxL_1	144	158	PF00134	0.407
DOC_CYCLIN_yCln2_LP_2	175	178	PF00134	0.475
DOC_MAPK_DCC_7	397	406	PF00069	0.426
DOC_MAPK_gen_1	130	137	PF00069	0.476
DOC_MAPK_gen_1	180	187	PF00069	0.439
DOC_MAPK_gen_1	428	438	PF00069	0.484
DOC_MAPK_gen_1	452	460	PF00069	0.346
DOC_MAPK_gen_1	98	105	PF00069	0.436
DOC_MAPK_HePTP_8	394	406	PF00069	0.426
DOC_MAPK_MEF2A_6	347	355	PF00069	0.415
DOC_MAPK_MEF2A_6	397	406	PF00069	0.426
DOC_PP2B_LxvP_1	175	178	PF13499	0.456
DOC_PP2B_LxvP_1	198	201	PF13499	0.643
DOC_PP2B_LxvP_1	314	317	PF13499	0.335
DOC_PP2B_LxvP_1	399	402	PF13499	0.405
DOC_PP4_FxxP_1	303	306	PF00568	0.513
DOC_PP4_FxxP_1	56	59	PF00568	0.415
DOC_PP4_FxxP_1	93	96	PF00568	0.475
DOC_USP7_MATH_1	208	212	PF00917	0.687
DOC_USP7_MATH_1	275	279	PF00917	0.674
DOC_USP7_MATH_1	287	291	PF00917	0.715
DOC_USP7_MATH_1	361	365	PF00917	0.415
DOC_USP7_MATH_1	402	406	PF00917	0.518
DOC_USP7_MATH_1	466	470	PF00917	0.520
DOC_USP7_MATH_1	47	51	PF00917	0.439
DOC_USP7_MATH_1	513	517	PF00917	0.594
DOC_USP7_MATH_1	538	542	PF00917	0.700
DOC_USP7_MATH_1	557	561	PF00917	0.732
DOC_USP7_MATH_1	591	595	PF00917	0.726
DOC_USP7_MATH_1	604	608	PF00917	0.540
DOC_USP7_UBL2_3	576	580	PF12436	0.608
DOC_WW_Pin1_4	124	129	PF00397	0.415
DOC_WW_Pin1_4	21	26	PF00397	0.379
DOC_WW_Pin1_4	224	229	PF00397	0.731
DOC_WW_Pin1_4	460	465	PF00397	0.479
DOC_WW_Pin1_4	516	521	PF00397	0.643
DOC_WW_Pin1_4	544	549	PF00397	0.722
DOC_WW_Pin1_4	612	617	PF00397	0.750
DOC_WW_Pin1_4	74	79	PF00397	0.415
LIG_14-3-3_CanoR_1	11	20	PF00244	0.400
LIG_14-3-3_CanoR_1	159	166	PF00244	0.445
LIG_14-3-3_CanoR_1	194	199	PF00244	0.477
LIG_14-3-3_CanoR_1	299	304	PF00244	0.553
LIG_14-3-3_CanoR_1	352	356	PF00244	0.415
LIG_14-3-3_CanoR_1	559	568	PF00244	0.645
LIG_14-3-3_CanoR_1	595	603	PF00244	0.674
LIG_14-3-3_CanoR_1	98	103	PF00244	0.436
LIG_APCC_ABBA_1	406	411	PF00400	0.426
LIG_BIR_III_4	67	71	PF00653	0.520
LIG_eIF4E_1	69	75	PF01652	0.415
LIG_FHA_1	186	192	PF00498	0.426
LIG_FHA_1	256	262	PF00498	0.411
LIG_FHA_1	352	358	PF00498	0.415
LIG_FHA_1	455	461	PF00498	0.377
LIG_FHA_2	110	116	PF00498	0.470
LIG_FHA_2	159	165	PF00498	0.415
LIG_FHA_2	194	200	PF00498	0.450
LIG_FHA_2	438	444	PF00498	0.326
LIG_FHA_2	561	567	PF00498	0.718
LIG_LIR_Apic_2	301	306	PF02991	0.534
LIG_LIR_Apic_2	459	464	PF02991	0.516
LIG_LIR_Apic_2	53	59	PF02991	0.415
LIG_LIR_Gen_1	18	27	PF02991	0.337
LIG_LIR_Gen_1	2	9	PF02991	0.351
LIG_LIR_Gen_1	243	253	PF02991	0.516
LIG_LIR_Gen_1	310	320	PF02991	0.401
LIG_LIR_Gen_1	81	92	PF02991	0.415
LIG_LIR_Nem_3	18	22	PF02991	0.337
LIG_LIR_Nem_3	2	7	PF02991	0.337
LIG_LIR_Nem_3	243	248	PF02991	0.539
LIG_LIR_Nem_3	310	316	PF02991	0.394
LIG_LIR_Nem_3	67	72	PF02991	0.414
LIG_LIR_Nem_3	81	87	PF02991	0.393
LIG_LIR_Nem_3	88	93	PF02991	0.405
LIG_LYPXL_SIV_4	323	331	PF13949	0.520
LIG_NRBOX	130	136	PF00104	0.475
LIG_NRBOX	364	370	PF00104	0.501
LIG_PDZ_Class_3	620	625	PF00595	0.595
LIG_RPA_C_Fungi	501	513	PF08784	0.410
LIG_RPA_C_Plants	496	507	PF08784	0.609
LIG_SH2_CRK	410	414	PF00017	0.267
LIG_SH2_GRB2like	341	344	PF00017	0.387
LIG_SH2_SRC	341	344	PF00017	0.385
LIG_SH2_SRC	84	87	PF00017	0.302
LIG_SH2_STAP1	442	446	PF00017	0.374
LIG_SH2_STAT5	151	154	PF00017	0.387
LIG_SH2_STAT5	313	316	PF00017	0.323
LIG_SH2_STAT5	324	327	PF00017	0.267
LIG_SH2_STAT5	341	344	PF00017	0.262
LIG_SH2_STAT5	419	422	PF00017	0.262
LIG_SH3_2	294	299	PF14604	0.608
LIG_SH3_3	134	140	PF00018	0.334
LIG_SH3_3	291	297	PF00018	0.610
LIG_SH3_3	317	323	PF00018	0.433
LIG_SH3_3	72	78	PF00018	0.267
LIG_SUMO_SIM_anti_2	260	265	PF11976	0.480
LIG_SUMO_SIM_anti_2	434	441	PF11976	0.365
LIG_SUMO_SIM_anti_2	443	448	PF11976	0.345
LIG_SUMO_SIM_par_1	468	477	PF11976	0.470
LIG_TRAF2_1	267	270	PF00917	0.456
LIG_TYR_ITIM	149	154	PF00017	0.387
LIG_TYR_ITIM	82	87	PF00017	0.267
LIG_WRC_WIRS_1	378	383	PF05994	0.283
MOD_CDC14_SPxK_1	615	618	PF00782	0.743
MOD_CDK_SPK_2	516	521	PF00069	0.505
MOD_CDK_SPxK_1	124	130	PF00069	0.267
MOD_CDK_SPxK_1	612	618	PF00069	0.744
MOD_CDK_SPxK_1	74	80	PF00069	0.267
MOD_CDK_SPxxK_3	460	467	PF00069	0.481
MOD_CDK_SPxxK_3	516	523	PF00069	0.629
MOD_CDK_SPxxK_3	612	619	PF00069	0.706
MOD_CK1_1	224	230	PF00069	0.669
MOD_CK1_1	278	284	PF00069	0.679
MOD_CK1_1	289	295	PF00069	0.650
MOD_CK1_1	364	370	PF00069	0.267
MOD_CK1_1	377	383	PF00069	0.267
MOD_CK1_1	50	56	PF00069	0.266
MOD_CK1_1	516	522	PF00069	0.617
MOD_CK1_1	547	553	PF00069	0.640
MOD_CK1_1	560	566	PF00069	0.632
MOD_CK1_1	594	600	PF00069	0.751
MOD_CK1_1	612	618	PF00069	0.661
MOD_CK2_1	193	199	PF00069	0.427
MOD_CK2_1	254	260	PF00069	0.472
MOD_GlcNHglycan	210	213	PF01048	0.732
MOD_GlcNHglycan	277	280	PF01048	0.681
MOD_GlcNHglycan	289	292	PF01048	0.679
MOD_GlcNHglycan	468	471	PF01048	0.414
MOD_GlcNHglycan	479	482	PF01048	0.291
MOD_GlcNHglycan	49	52	PF01048	0.275
MOD_GlcNHglycan	515	518	PF01048	0.494
MOD_GlcNHglycan	532	535	PF01048	0.735
MOD_GlcNHglycan	551	554	PF01048	0.692
MOD_GlcNHglycan	559	562	PF01048	0.715
MOD_GlcNHglycan	596	599	PF01048	0.697
MOD_GlcNHglycan	606	609	PF01048	0.674
MOD_GSK3_1	11	18	PF00069	0.467
MOD_GSK3_1	251	258	PF00069	0.448
MOD_GSK3_1	456	463	PF00069	0.490
MOD_GSK3_1	466	473	PF00069	0.506
MOD_GSK3_1	538	545	PF00069	0.672
MOD_LATS_1	192	198	PF00433	0.499
MOD_N-GLC_1	536	541	PF02516	0.662
MOD_N-GLC_2	343	345	PF02516	0.267
MOD_NEK2_1	185	190	PF00069	0.270
MOD_NEK2_1	222	227	PF00069	0.608
MOD_NEK2_1	542	547	PF00069	0.745
MOD_PIKK_1	109	115	PF00454	0.312
MOD_PIKK_1	307	313	PF00454	0.429
MOD_PIKK_1	367	373	PF00454	0.283
MOD_PIKK_1	566	572	PF00454	0.693
MOD_PIKK_1	596	602	PF00454	0.636
MOD_PK_1	374	380	PF00069	0.200
MOD_PKA_1	507	513	PF00069	0.340
MOD_PKA_2	158	164	PF00069	0.267
MOD_PKA_2	193	199	PF00069	0.425
MOD_PKA_2	298	304	PF00069	0.567
MOD_PKA_2	351	357	PF00069	0.267
MOD_PKA_2	557	563	PF00069	0.716
MOD_PKA_2	594	600	PF00069	0.691
MOD_Plk_1	254	260	PF00069	0.511
MOD_Plk_1	334	340	PF00069	0.289
MOD_Plk_1	442	448	PF00069	0.410
MOD_Plk_2-3	158	164	PF00069	0.332
MOD_Plk_2-3	255	261	PF00069	0.452
MOD_Plk_4	15	21	PF00069	0.486
MOD_Plk_4	168	174	PF00069	0.340
MOD_Plk_4	335	341	PF00069	0.254
MOD_Plk_4	364	370	PF00069	0.262
MOD_Plk_4	402	408	PF00069	0.362
MOD_Plk_4	415	421	PF00069	0.205
MOD_Plk_4	442	448	PF00069	0.397
MOD_Plk_4	456	462	PF00069	0.298
MOD_Plk_4	470	476	PF00069	0.364
MOD_ProDKin_1	124	130	PF00069	0.267
MOD_ProDKin_1	21	27	PF00069	0.380
MOD_ProDKin_1	224	230	PF00069	0.733
MOD_ProDKin_1	460	466	PF00069	0.477
MOD_ProDKin_1	516	522	PF00069	0.642
MOD_ProDKin_1	544	550	PF00069	0.723
MOD_ProDKin_1	612	618	PF00069	0.752
MOD_ProDKin_1	74	80	PF00069	0.267
MOD_SUMO_rev_2	445	454	PF00179	0.385
TRG_DiLeu_BaEn_1	243	248	PF01217	0.539
TRG_DiLeu_BaLyEn_6	171	176	PF01217	0.385
TRG_ENDOCYTIC_2	151	154	PF00928	0.387
TRG_ENDOCYTIC_2	19	22	PF00928	0.368
TRG_ENDOCYTIC_2	313	316	PF00928	0.323
TRG_ENDOCYTIC_2	69	72	PF00928	0.266
TRG_ENDOCYTIC_2	84	87	PF00928	0.218
TRG_ER_diArg_1	520	523	PF00400	0.640
TRG_ER_diArg_1	556	559	PF00400	0.671
TRG_ER_diArg_1	618	621	PF00400	0.751
TRG_ER_diArg_1	97	100	PF00400	0.297
TRG_NLS_Bipartite_1	558	580	PF00514	0.689
TRG_NLS_Bipartite_1	605	622	PF00514	0.711
TRG_NLS_MonoExtC_3	554	559	PF00514	0.629
TRG_NLS_MonoExtC_3	575	580	PF00514	0.647
TRG_NLS_MonoExtC_3	617	622	PF00514	0.710
TRG_NLS_MonoExtN_4	616	622	PF00514	0.708
TRG_Pf-PMV_PEXEL_1	11	15	PF00026	0.391
TRG_Pf-PMV_PEXEL_1	150	155	PF00026	0.206
TRG_Pf-PMV_PEXEL_1	585	590	PF00026	0.659
TRG_Pf-PMV_PEXEL_1	80	85	PF00026	0.267

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I2T9	Leptomonas seymouri	72%	100%
A0A0S4J5D6	Bodo saltans	49%	100%
A0A0S4KKU4	Bodo saltans	30%	90%
A0A1X0NJH3	Trypanosomatidae	26%	100%
A0A1X0P0F9	Trypanosomatidae	56%	100%
A0A1X0P7J3	Trypanosomatidae	23%	89%
A0A3R7M1K3	Trypanosoma rangeli	27%	87%
A0A3S5H5H4	Leishmania donovani	100%	100%
A0A3S5H6T7	Leishmania donovani	29%	100%
A0A3S7X5R1	Leishmania donovani	29%	81%
A0A422NDK5	Trypanosoma rangeli	54%	100%
A2XVF7	Oryza sativa subsp. indica	30%	75%
A3AVH5	Oryza sativa subsp. japonica	30%	75%
A4H481	Leishmania braziliensis	80%	100%
A4HWB0	Leishmania infantum	29%	100%
A4I846	Leishmania infantum	29%	81%
A4R5B8	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	32%	68%
A7ESL7	Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1)	33%	68%
A7TJ71	Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17)	27%	81%
C9ZNU6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	98%
D0A4H6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	100%
D0AAB3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	86%
E9AKE2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	99%
E9AQ10	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
E9B304	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	81%
P26802	Drosophila melanogaster	29%	91%
Q0CNX1	Aspergillus terreus (strain NIH 2624 / FGSC A1156)	38%	100%
Q1EB38	Coccidioides immitis (strain RS)	32%	67%
Q4HZ42	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	31%	70%
Q4Q552	Leishmania major	28%	81%
Q4QFH1	Leishmania major	30%	100%
Q4QJG6	Leishmania major	95%	100%
Q54BD6	Dictyostelium discoideum	25%	100%
Q6C835	Yarrowia lipolytica (strain CLIB 122 / E 150)	29%	78%
Q6FU81	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	27%	94%
Q7XJN0	Arabidopsis thaliana	28%	100%
Q8N8A6	Homo sapiens	28%	94%
Q93Y39	Arabidopsis thaliana	32%	76%
V5CZW7	Trypanosoma cruzi	58%	100%
V5DCA1	Trypanosoma cruzi	27%	87%