Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase

Quick info Localization Phosphorylation Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

FtsJ-like methyltransferase - putative

Species:

Leishmania infantum

UniProt:

A4HRN5_LEIIN

TriTrypDb:

LINF_020008800

Length:

457

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 13
NetGPI	no	yes: 0, no: 13

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	10
GO:0110165	cellular anatomical entity	1	11
GO:0005730	nucleolus	5	1
GO:0030684	preribosome	3	1
GO:0030687	preribosome, large subunit precursor	4	1
GO:0032991	protein-containing complex	1	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:1990904	ribonucleoprotein complex	2	1

Phosphorylation

Amastigote: 134
Promastigote: 145, 147
Promastigote/Amastigote: 148

Expansion

Sequence features

A4HRN5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HRN5

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	11
GO:0002128	tRNA nucleoside ribose methylation	6	10
GO:0002181	cytoplasmic translation	5	10
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006396	RNA processing	6	11
GO:0006399	tRNA metabolic process	7	10
GO:0006400	tRNA modification	6	10
GO:0006412	translation	4	10
GO:0006518	peptide metabolic process	4	10
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008033	tRNA processing	8	10
GO:0008152	metabolic process	1	14
GO:0009058	biosynthetic process	2	10
GO:0009059	macromolecule biosynthetic process	4	10
GO:0009451	RNA modification	5	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0019538	protein metabolic process	3	10
GO:0030488	tRNA methylation	5	10
GO:0032259	methylation	2	14
GO:0034470	ncRNA processing	7	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034645	obsolete cellular macromolecule biosynthetic process	4	10
GO:0034660	ncRNA metabolic process	6	11
GO:0043043	peptide biosynthetic process	5	10
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0043414	macromolecule methylation	3	11
GO:0043603	amide metabolic process	3	10
GO:0043604	amide biosynthetic process	4	10
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	10
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	10
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901564	organonitrogen compound metabolic process	3	10
GO:1901566	organonitrogen compound biosynthetic process	4	10
GO:1901576	organic substance biosynthetic process	3	10
GO:0000154	rRNA modification	6	1
GO:0000460	maturation of 5.8S rRNA	9	1
GO:0000463	maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	10	1
GO:0000466	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	10	1
GO:0000470	maturation of LSU-rRNA	9	1
GO:0006364	rRNA processing	8	1
GO:0016072	rRNA metabolic process	7	1
GO:0031167	rRNA methylation	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	14
GO:0008168	methyltransferase activity	4	14
GO:0008173	RNA methyltransferase activity	4	11
GO:0008175	tRNA methyltransferase activity	5	10
GO:0016740	transferase activity	2	14
GO:0016741	transferase activity, transferring one-carbon groups	3	14
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140101	catalytic activity, acting on a tRNA	4	10
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:0008171	O-methyltransferase activity	5	1
GO:0008649	rRNA methyltransferase activity	5	1
GO:0008650	rRNA (uridine-2'-O-)-methyltransferase activity	6	1
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	1
GO:0016435	rRNA (guanine) methyltransferase activity	6	1
GO:0016436	rRNA (uridine) methyltransferase activity	6	1
GO:0062105	RNA 2'-O-methyltransferase activity	5	1
GO:0140102	catalytic activity, acting on a rRNA	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	309	313	PF00656	0.544
CLV_C14_Caspase3-7	419	423	PF00656	0.624
CLV_C14_Caspase3-7	426	430	PF00656	0.610
CLV_NRD_NRD_1	13	15	PF00675	0.377
CLV_NRD_NRD_1	159	161	PF00675	0.282
CLV_PCSK_KEX2_1	8	10	PF00082	0.433
CLV_PCSK_PC1ET2_1	8	10	PF00082	0.433
CLV_PCSK_SKI1_1	14	18	PF00082	0.359
CLV_PCSK_SKI1_1	230	234	PF00082	0.315
DEG_APCC_DBOX_1	251	259	PF00400	0.396
DEG_SPOP_SBC_1	281	285	PF00917	0.748
DOC_CYCLIN_yCln2_LP_2	402	408	PF00134	0.551
DOC_MAPK_gen_1	160	166	PF00069	0.458
DOC_PP2B_LxvP_1	123	126	PF13499	0.524
DOC_PP2B_LxvP_1	402	405	PF13499	0.503
DOC_PP4_FxxP_1	146	149	PF00568	0.405
DOC_PP4_FxxP_1	210	213	PF00568	0.442
DOC_PP4_FxxP_1	251	254	PF00568	0.353
DOC_USP7_MATH_1	141	145	PF00917	0.556
DOC_USP7_MATH_1	234	238	PF00917	0.488
DOC_USP7_MATH_1	268	272	PF00917	0.636
DOC_USP7_MATH_1	282	286	PF00917	0.753
DOC_USP7_MATH_1	308	312	PF00917	0.622
DOC_USP7_MATH_1	412	416	PF00917	0.552
DOC_USP7_MATH_1	428	432	PF00917	0.495
DOC_WW_Pin1_4	124	129	PF00397	0.471
DOC_WW_Pin1_4	145	150	PF00397	0.542
DOC_WW_Pin1_4	155	160	PF00397	0.477
DOC_WW_Pin1_4	318	323	PF00397	0.653
DOC_WW_Pin1_4	410	415	PF00397	0.456
DOC_WW_Pin1_4	437	442	PF00397	0.587
DOC_WW_Pin1_4	83	88	PF00397	0.484
LIG_14-3-3_CanoR_1	211	219	PF00244	0.542
LIG_14-3-3_CanoR_1	22	30	PF00244	0.472
LIG_14-3-3_CanoR_1	238	243	PF00244	0.442
LIG_Actin_WH2_2	185	202	PF00022	0.458
LIG_APCC_ABBA_1	30	35	PF00400	0.434
LIG_BRCT_BRCA1_1	221	225	PF00533	0.461
LIG_eIF4E_1	182	188	PF01652	0.442
LIG_FHA_1	109	115	PF00498	0.505
LIG_FHA_1	193	199	PF00498	0.471
LIG_FHA_1	213	219	PF00498	0.475
LIG_FHA_1	305	311	PF00498	0.560
LIG_FHA_1	44	50	PF00498	0.453
LIG_FHA_1	84	90	PF00498	0.480
LIG_FHA_2	113	119	PF00498	0.442
LIG_FHA_2	132	138	PF00498	0.471
LIG_FHA_2	307	313	PF00498	0.534
LIG_FHA_2	346	352	PF00498	0.585
LIG_FHA_2	424	430	PF00498	0.578
LIG_FHA_2	449	455	PF00498	0.590
LIG_LIR_Apic_2	144	149	PF02991	0.405
LIG_LIR_Apic_2	207	213	PF02991	0.442
LIG_LIR_Gen_1	34	44	PF02991	0.460
LIG_LIR_Gen_1	57	66	PF02991	0.458
LIG_LIR_Nem_3	25	30	PF02991	0.460
LIG_LIR_Nem_3	34	39	PF02991	0.490
LIG_LIR_Nem_3	57	61	PF02991	0.458
LIG_PCNA_PIPBox_1	176	185	PF02747	0.458
LIG_PCNA_yPIPBox_3	233	246	PF02747	0.442
LIG_SH2_CRK	27	31	PF00017	0.442
LIG_SH2_STAP1	329	333	PF00017	0.598
LIG_SH2_STAT5	182	185	PF00017	0.442
LIG_SH2_STAT5	257	260	PF00017	0.479
LIG_SH2_STAT5	327	330	PF00017	0.650
LIG_SH2_STAT5	406	409	PF00017	0.478
LIG_SH3_3	228	234	PF00018	0.541
LIG_SH3_3	319	325	PF00018	0.562
LIG_SH3_3	364	370	PF00018	0.463
LIG_SH3_3	391	397	PF00018	0.484
LIG_SH3_3	435	441	PF00018	0.672
LIG_SUMO_SIM_anti_2	101	107	PF11976	0.472
LIG_SUMO_SIM_par_1	88	93	PF11976	0.472
LIG_TRAF2_1	16	19	PF00917	0.357
LIG_WRC_WIRS_1	194	199	PF05994	0.442
LIG_WRC_WIRS_1	242	247	PF05994	0.524
MOD_CDC14_SPxK_1	158	161	PF00782	0.498
MOD_CDK_SPK_2	155	160	PF00069	0.498
MOD_CDK_SPK_2	83	88	PF00069	0.472
MOD_CDK_SPxK_1	155	161	PF00069	0.498
MOD_CK1_1	112	118	PF00069	0.459
MOD_CK1_1	144	150	PF00069	0.515
MOD_CK1_1	241	247	PF00069	0.524
MOD_CK1_1	272	278	PF00069	0.670
MOD_CK1_1	318	324	PF00069	0.572
MOD_CK1_1	336	342	PF00069	0.594
MOD_CK2_1	112	118	PF00069	0.442
MOD_CK2_1	131	137	PF00069	0.541
MOD_CK2_1	287	293	PF00069	0.663
MOD_CK2_1	448	454	PF00069	0.591
MOD_DYRK1A_RPxSP_1	437	441	PF00069	0.586
MOD_GlcNHglycan	221	224	PF01048	0.243
MOD_GlcNHglycan	24	27	PF01048	0.276
MOD_GlcNHglycan	271	274	PF01048	0.671
MOD_GlcNHglycan	284	287	PF01048	0.693
MOD_GlcNHglycan	317	320	PF01048	0.680
MOD_GlcNHglycan	376	380	PF01048	0.506
MOD_GSK3_1	108	115	PF00069	0.448
MOD_GSK3_1	141	148	PF00069	0.496
MOD_GSK3_1	234	241	PF00069	0.474
MOD_GSK3_1	268	275	PF00069	0.587
MOD_GSK3_1	302	309	PF00069	0.751
MOD_GSK3_1	329	336	PF00069	0.649
MOD_GSK3_1	437	444	PF00069	0.552
MOD_N-GLC_1	142	147	PF02516	0.329
MOD_N-GLC_1	238	243	PF02516	0.242
MOD_N-GLC_1	71	76	PF02516	0.260
MOD_NEK2_1	192	197	PF00069	0.442
MOD_NEK2_1	315	320	PF00069	0.651
MOD_NEK2_2	345	350	PF00069	0.557
MOD_PIKK_1	412	418	PF00454	0.689
MOD_PIKK_1	428	434	PF00454	0.523
MOD_PIKK_1	56	62	PF00454	0.442
MOD_PKA_2	2	8	PF00069	0.488
MOD_PKA_2	315	321	PF00069	0.633
MOD_PKA_2	357	363	PF00069	0.675
MOD_PKA_2	423	429	PF00069	0.594
MOD_PKA_2	446	452	PF00069	0.591
MOD_Plk_1	142	148	PF00069	0.500
MOD_Plk_1	238	244	PF00069	0.442
MOD_Plk_2-3	306	312	PF00069	0.530
MOD_Plk_4	101	107	PF00069	0.448
MOD_Plk_4	193	199	PF00069	0.466
MOD_Plk_4	241	247	PF00069	0.538
MOD_ProDKin_1	124	130	PF00069	0.471
MOD_ProDKin_1	145	151	PF00069	0.542
MOD_ProDKin_1	155	161	PF00069	0.477
MOD_ProDKin_1	318	324	PF00069	0.645
MOD_ProDKin_1	410	416	PF00069	0.464
MOD_ProDKin_1	437	443	PF00069	0.586
MOD_ProDKin_1	83	89	PF00069	0.484
MOD_SUMO_for_1	16	19	PF00179	0.357
MOD_SUMO_for_1	300	303	PF00179	0.590
TRG_DiLeu_BaEn_1	118	123	PF01217	0.458
TRG_DiLeu_BaEn_4	34	40	PF01217	0.458
TRG_DiLeu_LyEn_5	118	123	PF01217	0.458
TRG_ENDOCYTIC_2	27	30	PF00928	0.460
TRG_ER_diArg_1	21	24	PF00400	0.449
TRG_NES_CRM1_1	95	109	PF08389	0.442
TRG_NLS_MonoExtC_3	432	437	PF00514	0.628
TRG_NLS_MonoExtN_4	433	438	PF00514	0.677

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HTF7	Leptomonas seymouri	61%	100%
A0A0S4JD71	Bodo saltans	45%	100%
A0A3S7X469	Leishmania donovani	24%	100%
A0A451EJH4	Leishmania donovani	100%	100%
A4H3D4	Leishmania braziliensis	76%	99%
A4I6F9	Leishmania infantum	24%	100%
C9ZJ17	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	45%	100%
E9ACB3	Leishmania major	95%	100%
E9AJK1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	99%
E9B1L6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	100%
Q9BHC8	Leishmania major	24%	100%
V5BCX7	Trypanosoma cruzi	50%	100%