Alpha/beta-hydrolase-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

nicotinamidase - putative

Species:

Leishmania infantum

UniProt:

A4HRG8_LEIIN

TriTrypDb:

LINF_010009600

Length:

218

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005829	cytosol	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HRG8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HRG8

PDB structure(s): 3r2j_A , 3r2j_B , 3r2j_C , 3r2j_D

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006753	nucleoside phosphate metabolic process	4	1
GO:0006793	phosphorus metabolic process	3	1
GO:0006796	phosphate-containing compound metabolic process	4	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009117	nucleotide metabolic process	5	1
GO:0009165	nucleotide biosynthetic process	6	1
GO:0009435	NAD biosynthetic process	8	1
GO:0009987	cellular process	1	1
GO:0018130	heterocycle biosynthetic process	4	1
GO:0019359	nicotinamide nucleotide biosynthetic process	7	1
GO:0019362	pyridine nucleotide metabolic process	5	1
GO:0019363	pyridine nucleotide biosynthetic process	6	1
GO:0019438	aromatic compound biosynthetic process	4	1
GO:0019637	organophosphate metabolic process	3	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034654	nucleobase-containing compound biosynthetic process	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0044281	small molecule metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0046496	nicotinamide nucleotide metabolic process	6	1
GO:0055086	nucleobase-containing small molecule metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0072524	pyridine-containing compound metabolic process	4	1
GO:0072525	pyridine-containing compound biosynthetic process	5	1
GO:0090407	organophosphate biosynthetic process	4	1
GO:1901293	nucleoside phosphate biosynthetic process	5	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901566	organonitrogen compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	6
GO:0005488	binding	1	2
GO:0016787	hydrolase activity	2	6
GO:0043167	ion binding	2	2
GO:0043169	cation binding	3	2
GO:0046872	metal ion binding	4	2
GO:0008936	nicotinamidase activity	5	1
GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	3	1
GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	165	169	PF00656	0.469
CLV_C14_Caspase3-7	197	201	PF00656	0.415
CLV_NRD_NRD_1	115	117	PF00675	0.345
CLV_NRD_NRD_1	149	151	PF00675	0.287
CLV_NRD_NRD_1	169	171	PF00675	0.147
CLV_PCSK_KEX2_1	149	151	PF00082	0.287
CLV_PCSK_SKI1_1	117	121	PF00082	0.272
CLV_PCSK_SKI1_1	149	153	PF00082	0.287
DEG_SPOP_SBC_1	21	25	PF00917	0.312
DOC_MAPK_DCC_7	10	19	PF00069	0.510
DOC_MAPK_gen_1	170	178	PF00069	0.525
DOC_MAPK_MEF2A_6	10	19	PF00069	0.510
DOC_PP4_FxxP_1	37	40	PF00568	0.469
DOC_USP7_MATH_1	40	44	PF00917	0.494
DOC_USP7_MATH_1	58	62	PF00917	0.560
LIG_14-3-3_CanoR_1	116	126	PF00244	0.540
LIG_CSL_BTD_1	88	91	PF09270	0.453
LIG_FHA_1	106	112	PF00498	0.551
LIG_FHA_1	14	20	PF00498	0.553
LIG_FHA_1	181	187	PF00498	0.483
LIG_FHA_1	193	199	PF00498	0.478
LIG_FHA_1	22	28	PF00498	0.419
LIG_FHA_2	163	169	PF00498	0.469
LIG_FHA_2	3	9	PF00498	0.663
LIG_LIR_Apic_2	36	40	PF02991	0.469
LIG_LIR_Gen_1	124	130	PF02991	0.483
LIG_LIR_Gen_1	159	167	PF02991	0.521
LIG_LIR_Nem_3	124	129	PF02991	0.466
LIG_LIR_Nem_3	159	163	PF02991	0.469
LIG_NRBOX	138	144	PF00104	0.487
LIG_Pex14_2	160	164	PF04695	0.540
LIG_SH2_STAP1	126	130	PF00017	0.504
LIG_SH2_STAP1	158	162	PF00017	0.504
LIG_SH3_3	6	12	PF00018	0.519
LIG_SH3_3	85	91	PF00018	0.460
LIG_SUMO_SIM_anti_2	175	181	PF11976	0.413
LIG_SUMO_SIM_anti_2	22	32	PF11976	0.322
LIG_SUMO_SIM_par_1	175	181	PF11976	0.457
LIG_SUMO_SIM_par_1	202	209	PF11976	0.545
LIG_SUMO_SIM_par_1	22	32	PF11976	0.321
MOD_CK1_1	192	198	PF00069	0.415
MOD_CK1_1	22	28	PF00069	0.437
MOD_CK1_1	81	87	PF00069	0.533
MOD_GlcNHglycan	126	129	PF01048	0.285
MOD_GlcNHglycan	191	194	PF01048	0.306
MOD_GlcNHglycan	208	211	PF01048	0.405
MOD_GlcNHglycan	96	99	PF01048	0.331
MOD_GSK3_1	13	20	PF00069	0.588
MOD_N-GLC_2	78	80	PF02516	0.345
MOD_NEK2_1	142	147	PF00069	0.550
MOD_NEK2_1	17	22	PF00069	0.514
MOD_NEK2_1	205	210	PF00069	0.551
MOD_NEK2_2	40	45	PF00069	0.494
MOD_PIKK_1	105	111	PF00454	0.448
MOD_PIKK_1	117	123	PF00454	0.458
MOD_PIKK_1	58	64	PF00454	0.565
MOD_PIKK_1	68	74	PF00454	0.523
MOD_Plk_4	78	84	PF00069	0.540
MOD_SUMO_rev_2	192	201	PF00179	0.549
TRG_ENDOCYTIC_2	126	129	PF00928	0.455
TRG_ER_diArg_1	149	151	PF00400	0.490

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P300	Leptomonas seymouri	61%	100%
A0A1X0P2N6	Trypanosomatidae	46%	80%
A0A3S5H4V3	Leishmania donovani	100%	100%
A0A422NY51	Trypanosoma rangeli	45%	90%
A4H371	Leishmania braziliensis	77%	100%
C9ZXJ1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	45%	100%
E9AC36	Leishmania major	94%	100%
E9AJD2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	81%	100%
I6XD65	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	42%	100%
P21369	Escherichia coli (strain K12)	40%	100%
P53184	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	100%
Q9USS0	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	99%
V5BLL8	Trypanosoma cruzi	46%	98%