EF-hand domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Protein of unknown function (DUF2453), putative

Species:

Leishmania braziliensis

UniProt:

A4HQK4_LEIBR

TriTrypDb:

LbrM.35.7200 , LBRM2903_350085100

Length:

350

Annotations

LeishMANIAdb annotations

These Kinetoplastid proteins share considerable homology with the animal TMEM65 proteins at their membrane domains, with a unique EF-hand at the N-terminus.. While the animal orthologs have a mitochondrial transit signal, it seems to be absent from the Kinetoplastid proteins. The latter have an alanine-rich segment of unclear function.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 13
NetGPI	no	yes: 0, no: 13

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	14
GO:0110165	cellular anatomical entity	1	14
GO:0005739	mitochondrion	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

A4HQK4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HQK4

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	12
GO:0005509	calcium ion binding	5	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	186	190	PF00656	0.286
CLV_C14_Caspase3-7	341	345	PF00656	0.466
CLV_C14_Caspase3-7	90	94	PF00656	0.622
CLV_NRD_NRD_1	115	117	PF00675	0.394
CLV_NRD_NRD_1	244	246	PF00675	0.310
CLV_NRD_NRD_1	68	70	PF00675	0.367
CLV_PCSK_KEX2_1	114	116	PF00082	0.383
CLV_PCSK_KEX2_1	122	124	PF00082	0.438
CLV_PCSK_KEX2_1	244	246	PF00082	0.297
CLV_PCSK_KEX2_1	67	69	PF00082	0.364
CLV_PCSK_PC1ET2_1	114	116	PF00082	0.428
CLV_PCSK_PC1ET2_1	122	124	PF00082	0.479
CLV_PCSK_SKI1_1	122	126	PF00082	0.510
CLV_PCSK_SKI1_1	128	132	PF00082	0.548
CLV_PCSK_SKI1_1	244	248	PF00082	0.311
CLV_PCSK_SKI1_1	346	350	PF00082	0.656
DEG_SCF_FBW7_1	231	237	PF00400	0.556
DOC_CDC14_PxL_1	264	272	PF14671	0.421
DOC_CKS1_1	231	236	PF01111	0.540
DOC_CYCLIN_RxL_1	303	314	PF00134	0.452
DOC_MAPK_gen_1	5	14	PF00069	0.585
DOC_MAPK_RevD_3	229	245	PF00069	0.393
DOC_PP1_RVXF_1	327	333	PF00149	0.409
DOC_USP7_MATH_1	139	143	PF00917	0.751
DOC_USP7_MATH_1	30	34	PF00917	0.741
DOC_USP7_MATH_1	35	39	PF00917	0.691
DOC_WW_Pin1_4	230	235	PF00397	0.515
DOC_WW_Pin1_4	98	103	PF00397	0.610
LIG_14-3-3_CanoR_1	123	130	PF00244	0.688
LIG_BIR_II_1	1	5	PF00653	0.714
LIG_BRCT_BRCA1_1	190	194	PF00533	0.361
LIG_Clathr_ClatBox_1	269	273	PF01394	0.397
LIG_FHA_1	151	157	PF00498	0.643
LIG_FHA_1	178	184	PF00498	0.309
LIG_FHA_1	18	24	PF00498	0.726
LIG_FHA_1	231	237	PF00498	0.532
LIG_FHA_1	253	259	PF00498	0.346
LIG_FHA_2	129	135	PF00498	0.769
LIG_FHA_2	35	41	PF00498	0.641
LIG_GBD_Chelix_1	248	256	PF00786	0.281
LIG_LIR_Gen_1	13	21	PF02991	0.712
LIG_LIR_Gen_1	280	290	PF02991	0.425
LIG_LIR_Gen_1	300	308	PF02991	0.200
LIG_LIR_Gen_1	333	342	PF02991	0.407
LIG_LIR_Nem_3	13	17	PF02991	0.713
LIG_LIR_Nem_3	280	285	PF02991	0.430
LIG_LIR_Nem_3	300	304	PF02991	0.184
LIG_LIR_Nem_3	333	338	PF02991	0.371
LIG_Pex14_2	105	109	PF04695	0.596
LIG_SH2_GRB2like	195	198	PF00017	0.397
LIG_SH2_STAT5	195	198	PF00017	0.376
LIG_SH3_3	293	299	PF00018	0.431
LIG_SUMO_SIM_par_1	160	165	PF11976	0.417
LIG_TRAF2_1	85	88	PF00917	0.649
MOD_CDK_SPK_2	98	103	PF00069	0.611
MOD_CK1_1	13	19	PF00069	0.640
MOD_CK2_1	128	134	PF00069	0.775
MOD_CK2_1	34	40	PF00069	0.649
MOD_GlcNHglycan	110	113	PF01048	0.408
MOD_GlcNHglycan	137	140	PF01048	0.589
MOD_GlcNHglycan	202	205	PF01048	0.367
MOD_GlcNHglycan	210	213	PF01048	0.283
MOD_GlcNHglycan	32	35	PF01048	0.472
MOD_GlcNHglycan	83	86	PF01048	0.422
MOD_GSK3_1	13	20	PF00069	0.667
MOD_GSK3_1	135	142	PF00069	0.739
MOD_GSK3_1	196	203	PF00069	0.421
MOD_GSK3_1	21	28	PF00069	0.693
MOD_GSK3_1	230	237	PF00069	0.519
MOD_GSK3_1	273	280	PF00069	0.428
MOD_GSK3_1	30	37	PF00069	0.670
MOD_N-GLC_1	177	182	PF02516	0.538
MOD_N-GLC_1	196	201	PF02516	0.197
MOD_N-GLC_2	208	210	PF02516	0.451
MOD_NEK2_1	1	6	PF00069	0.740
MOD_NEK2_1	176	181	PF00069	0.379
MOD_NEK2_1	196	201	PF00069	0.138
MOD_NEK2_1	252	257	PF00069	0.381
MOD_PKA_1	122	128	PF00069	0.748
MOD_PKA_2	122	128	PF00069	0.716
MOD_Plk_1	176	182	PF00069	0.334
MOD_Plk_1	196	202	PF00069	0.138
MOD_Plk_1	222	228	PF00069	0.393
MOD_Plk_4	177	183	PF00069	0.285
MOD_Plk_4	196	202	PF00069	0.197
MOD_ProDKin_1	230	236	PF00069	0.515
MOD_ProDKin_1	98	104	PF00069	0.604
MOD_SUMO_rev_2	341	348	PF00179	0.471
TRG_DiLeu_BaLyEn_6	265	270	PF01217	0.330
TRG_ENDOCYTIC_2	282	285	PF00928	0.425
TRG_ER_diArg_1	244	246	PF00400	0.506
TRG_ER_diArg_1	67	69	PF00400	0.563
TRG_NLS_MonoExtC_3	120	126	PF00514	0.723
TRG_Pf-PMV_PEXEL_1	303	307	PF00026	0.610
TRG_Pf-PMV_PEXEL_1	5	9	PF00026	0.559
TRG_Pf-PMV_PEXEL_1	53	57	PF00026	0.461

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P806	Leptomonas seymouri	78%	99%
A0A0S4J5S5	Bodo saltans	30%	100%
A0A0S4J8V3	Bodo saltans	35%	100%
A0A0S4JY67	Bodo saltans	52%	100%
A0A1X0NL71	Trypanosomatidae	55%	100%
A0A3R7NFF7	Trypanosoma rangeli	29%	90%
A0A3S7XCB7	Leishmania donovani	85%	100%
A4ICC4	Leishmania infantum	85%	100%
E9AUB9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	100%
Q4Q071	Leishmania major	85%	100%
V5BGX0	Trypanosoma cruzi	34%	84%
V5BHN9	Trypanosoma cruzi	55%	100%