Methylenetetrahydrofolate reductase (NAD(P)H)

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

methylenetetrahydrofolate reductase, putative

Species:

Leishmania braziliensis

UniProt:

A4HQF9_LEIBR

TriTrypDb:

LbrM.35.6720 , LBRM2903_350080000

Length:

309

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005829	cytosol	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HQF9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HQF9

Function

Biological processes
Term	Name	Level	Count
GO:0000096	sulfur amino acid metabolic process	4	9
GO:0006082	organic acid metabolic process	3	9
GO:0006520	amino acid metabolic process	3	9
GO:0006555	methionine metabolic process	5	9
GO:0006575	cellular modified amino acid metabolic process	3	9
GO:0006725	cellular aromatic compound metabolic process	3	9
GO:0006730	one-carbon metabolic process	3	9
GO:0006760	folic acid-containing compound metabolic process	4	9
GO:0006790	sulfur compound metabolic process	3	9
GO:0006807	nitrogen compound metabolic process	2	9
GO:0008152	metabolic process	1	9
GO:0009066	aspartate family amino acid metabolic process	5	9
GO:0009987	cellular process	1	9
GO:0019752	carboxylic acid metabolic process	5	9
GO:0035999	tetrahydrofolate interconversion	4	9
GO:0042558	pteridine-containing compound metabolic process	4	9
GO:0043436	oxoacid metabolic process	4	9
GO:0044237	cellular metabolic process	2	9
GO:0044238	primary metabolic process	2	9
GO:0044281	small molecule metabolic process	2	9
GO:0046483	heterocycle metabolic process	3	9
GO:0046653	tetrahydrofolate metabolic process	5	9
GO:0071704	organic substance metabolic process	2	9
GO:1901360	organic cyclic compound metabolic process	3	9
GO:1901564	organonitrogen compound metabolic process	3	9
GO:1901605	alpha-amino acid metabolic process	4	9
GO:0000097	sulfur amino acid biosynthetic process	5	1
GO:0008652	amino acid biosynthetic process	4	1
GO:0009058	biosynthetic process	2	1
GO:0009067	aspartate family amino acid biosynthetic process	6	1
GO:0009086	methionine biosynthetic process	6	1
GO:0016053	organic acid biosynthetic process	4	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044272	sulfur compound biosynthetic process	4	1
GO:0044283	small molecule biosynthetic process	3	1
GO:0046394	carboxylic acid biosynthetic process	5	1
GO:1901566	organonitrogen compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1
GO:1901607	alpha-amino acid biosynthetic process	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	9
GO:0004489	methylenetetrahydrofolate reductase (NAD(P)H) activity	5	9
GO:0016491	oxidoreductase activity	2	9
GO:0016645	oxidoreductase activity, acting on the CH-NH group of donors	3	9
GO:0016646	oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor	4	9
GO:0000166	nucleotide binding	3	1
GO:0005488	binding	1	1
GO:0036094	small molecule binding	2	1
GO:0043167	ion binding	2	1
GO:0043168	anion binding	3	1
GO:0050660	flavin adenine dinucleotide binding	4	1
GO:0071949	FAD binding	5	1
GO:0097159	organic cyclic compound binding	2	1
GO:1901265	nucleoside phosphate binding	3	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	183	187	PF00656	0.319
CLV_PCSK_KEX2_1	249	251	PF00082	0.383
CLV_PCSK_KEX2_1	302	304	PF00082	0.356
CLV_PCSK_PC1ET2_1	249	251	PF00082	0.383
CLV_PCSK_PC1ET2_1	302	304	PF00082	0.356
CLV_PCSK_SKI1_1	108	112	PF00082	0.316
CLV_PCSK_SKI1_1	205	209	PF00082	0.311
DEG_APCC_KENBOX_2	254	258	PF00400	0.319
DOC_CKS1_1	77	82	PF01111	0.319
DOC_MAPK_gen_1	205	215	PF00069	0.265
DOC_MAPK_MEF2A_6	208	217	PF00069	0.299
DOC_MAPK_MEF2A_6	45	53	PF00069	0.229
DOC_PP4_FxxP_1	23	26	PF00568	0.188
DOC_USP7_MATH_1	187	191	PF00917	0.319
DOC_WW_Pin1_4	44	49	PF00397	0.338
DOC_WW_Pin1_4	76	81	PF00397	0.319
LIG_14-3-3_CanoR_1	234	239	PF00244	0.348
LIG_14-3-3_CanoR_1	60	68	PF00244	0.259
LIG_BIR_II_1	1	5	PF00653	0.398
LIG_BRCT_BRCA1_1	19	23	PF00533	0.188
LIG_BRCT_BRCA1_1	46	50	PF00533	0.288
LIG_EH1_1	104	112	PF00400	0.319
LIG_FHA_1	214	220	PF00498	0.348
LIG_FHA_1	231	237	PF00498	0.229
LIG_FHA_1	295	301	PF00498	0.225
LIG_FHA_1	63	69	PF00498	0.293
LIG_FHA_2	141	147	PF00498	0.348
LIG_FHA_2	166	172	PF00498	0.213
LIG_FHA_2	17	23	PF00498	0.348
LIG_Integrin_RGD_1	114	116	PF01839	0.254
LIG_Integrin_RGD_1	119	121	PF01839	0.254
LIG_LIR_Apic_2	20	26	PF02991	0.188
LIG_LIR_Gen_1	201	207	PF02991	0.288
LIG_LIR_Gen_1	282	291	PF02991	0.319
LIG_LIR_Gen_1	297	306	PF02991	0.180
LIG_LIR_Gen_1	32	43	PF02991	0.388
LIG_LIR_Gen_1	47	57	PF02991	0.272
LIG_LIR_Nem_3	143	147	PF02991	0.292
LIG_LIR_Nem_3	19	24	PF02991	0.311
LIG_LIR_Nem_3	201	206	PF02991	0.292
LIG_LIR_Nem_3	227	232	PF02991	0.332
LIG_LIR_Nem_3	282	286	PF02991	0.319
LIG_LIR_Nem_3	297	301	PF02991	0.180
LIG_LIR_Nem_3	32	38	PF02991	0.388
LIG_LIR_Nem_3	47	53	PF02991	0.315
LIG_Pex14_1	17	21	PF04695	0.348
LIG_SH2_SRC	139	142	PF00017	0.177
LIG_SH2_STAP1	198	202	PF00017	0.269
LIG_SH2_STAT5	139	142	PF00017	0.266
LIG_SH2_STAT5	147	150	PF00017	0.239
LIG_SH2_STAT5	18	21	PF00017	0.259
LIG_SH2_STAT5	180	183	PF00017	0.254
LIG_SH2_STAT5	203	206	PF00017	0.360
LIG_SH2_STAT5	289	292	PF00017	0.292
LIG_SH3_3	35	41	PF00018	0.348
LIG_SH3_3	74	80	PF00018	0.372
LIG_TYR_ITIM	281	286	PF00017	0.319
LIG_WW_3	116	120	PF00397	0.243
LIG_WW_3	24	28	PF00397	0.254
MOD_CK1_1	294	300	PF00069	0.265
MOD_CK1_1	62	68	PF00069	0.302
MOD_CK2_1	122	128	PF00069	0.304
MOD_CK2_1	16	22	PF00069	0.288
MOD_GlcNHglycan	1	4	PF01048	0.295
MOD_GlcNHglycan	278	281	PF01048	0.319
MOD_GlcNHglycan	293	296	PF01048	0.180
MOD_GSK3_1	187	194	PF00069	0.376
MOD_GSK3_1	230	237	PF00069	0.266
MOD_GSK3_1	287	294	PF00069	0.348
MOD_GSK3_1	58	65	PF00069	0.286
MOD_N-GLC_1	291	296	PF02516	0.348
MOD_N-GLC_1	88	93	PF02516	0.177
MOD_NEK2_1	148	153	PF00069	0.328
MOD_NEK2_1	213	218	PF00069	0.199
MOD_NEK2_1	238	243	PF00069	0.254
MOD_NEK2_1	291	296	PF00069	0.376
MOD_NEK2_1	51	56	PF00069	0.310
MOD_NEK2_1	83	88	PF00069	0.254
MOD_NEK2_2	198	203	PF00069	0.348
MOD_PIKK_1	191	197	PF00454	0.254
MOD_PKA_2	276	282	PF00069	0.322
MOD_PKA_2	59	65	PF00069	0.305
MOD_Plk_1	198	204	PF00069	0.348
MOD_Plk_1	51	57	PF00069	0.288
MOD_Plk_1	62	68	PF00069	0.254
MOD_Plk_2-3	165	171	PF00069	0.229
MOD_Plk_4	165	171	PF00069	0.362
MOD_Plk_4	187	193	PF00069	0.421
MOD_Plk_4	198	204	PF00069	0.362
MOD_ProDKin_1	44	50	PF00069	0.338
MOD_ProDKin_1	76	82	PF00069	0.319
TRG_ENDOCYTIC_2	147	150	PF00928	0.328
TRG_ENDOCYTIC_2	203	206	PF00928	0.374
TRG_ENDOCYTIC_2	229	232	PF00928	0.288
TRG_ENDOCYTIC_2	283	286	PF00928	0.319

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IKP8	Leptomonas seymouri	66%	100%
A0A0S4IQL6	Bodo saltans	53%	99%
A0A1X0NM13	Trypanosomatidae	57%	100%
A0A3Q8IJZ9	Leishmania donovani	87%	100%
A4IC95	Leishmania infantum	87%	100%
E9AU75	Leishmania mexicana (strain MHOM/GT/2001/U1103)	86%	100%
O54235	Streptomyces lividans	32%	100%
O67422	Aquifex aeolicus (strain VF5)	35%	100%
P0AEZ1	Escherichia coli (strain K12)	30%	100%
P0AEZ2	Shigella flexneri	30%	100%
P11003	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	30%	100%
P45208	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	30%	100%
P57154	Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)	30%	100%
P71319	Pectobacterium carotovorum subsp. carotovorum	31%	100%
Q4Q0B9	Leishmania major	86%	100%
Q89B13	Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)	29%	100%
Q8KA62	Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)	29%	100%
Q9JZQ3	Neisseria meningitidis serogroup B (strain MC58)	30%	100%