Ubiquitin thioesterase OTU1

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

OTU-like cysteine protease, putative

Species:

Leishmania braziliensis

UniProt:

A4HQC1_LEIBR

TriTrypDb:

LbrM.35.6330 , LBRM2903_350074700

Length:

370

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

A4HQC1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HQC1

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	12
GO:0006511	ubiquitin-dependent protein catabolic process	7	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	12
GO:0008152	metabolic process	1	12
GO:0009056	catabolic process	2	12
GO:0009057	macromolecule catabolic process	4	12
GO:0009987	cellular process	1	12
GO:0010033	response to organic substance	3	12
GO:0010243	response to organonitrogen compound	4	12
GO:0010498	proteasomal protein catabolic process	5	12
GO:0016579	protein deubiquitination	6	12
GO:0019538	protein metabolic process	3	12
GO:0019941	modification-dependent protein catabolic process	6	12
GO:0030163	protein catabolic process	4	12
GO:0030433	ubiquitin-dependent ERAD pathway	6	12
GO:0033554	cellular response to stress	3	12
GO:0034976	response to endoplasmic reticulum stress	4	12
GO:0036211	protein modification process	4	12
GO:0036503	ERAD pathway	5	12
GO:0042221	response to chemical	2	12
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0043632	modification-dependent macromolecule catabolic process	5	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044248	cellular catabolic process	3	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0044265	obsolete cellular macromolecule catabolic process	4	12
GO:0050896	response to stimulus	1	12
GO:0051603	proteolysis involved in protein catabolic process	5	12
GO:0051716	cellular response to stimulus	2	12
GO:0070646	protein modification by small protein removal	5	12
GO:0070647	protein modification by small protein conjugation or removal	5	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1901565	organonitrogen compound catabolic process	4	12
GO:1901575	organic substance catabolic process	3	12
GO:1901698	response to nitrogen compound	3	12
GO:0007165	signal transduction	2	1
GO:0030968	endoplasmic reticulum unfolded protein response	3	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0065007	biological regulation	1	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0004843	cysteine-type deubiquitinase activity	5	12
GO:0008233	peptidase activity	3	12
GO:0008234	cysteine-type peptidase activity	4	12
GO:0016787	hydrolase activity	2	12
GO:0019783	ubiquitin-like protein peptidase activity	4	12
GO:0101005	deubiquitinase activity	5	12
GO:0140096	catalytic activity, acting on a protein	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	267	271	PF00656	0.241
CLV_C14_Caspase3-7	31	35	PF00656	0.427
CLV_NRD_NRD_1	207	209	PF00675	0.323
CLV_NRD_NRD_1	55	57	PF00675	0.440
CLV_PCSK_FUR_1	352	356	PF00082	0.415
CLV_PCSK_KEX2_1	206	208	PF00082	0.333
CLV_PCSK_KEX2_1	354	356	PF00082	0.451
CLV_PCSK_KEX2_1	96	98	PF00082	0.498
CLV_PCSK_PC1ET2_1	354	356	PF00082	0.483
CLV_PCSK_PC1ET2_1	96	98	PF00082	0.390
CLV_PCSK_SKI1_1	184	188	PF00082	0.537
CLV_PCSK_SKI1_1	241	245	PF00082	0.417
CLV_PCSK_SKI1_1	30	34	PF00082	0.456
CLV_PCSK_SKI1_1	311	315	PF00082	0.398
CLV_PCSK_SKI1_1	56	60	PF00082	0.391
DEG_SCF_FBW7_1	109	115	PF00400	0.435
DEG_SPOP_SBC_1	112	116	PF00917	0.476
DOC_CKS1_1	109	114	PF01111	0.566
DOC_CYCLIN_RxL_1	91	104	PF00134	0.373
DOC_MAPK_FxFP_2	79	82	PF00069	0.387
DOC_MAPK_gen_1	110	120	PF00069	0.410
DOC_MAPK_MEF2A_6	113	122	PF00069	0.289
DOC_MAPK_MEF2A_6	96	103	PF00069	0.395
DOC_MAPK_NFAT4_5	96	104	PF00069	0.379
DOC_MAPK_RevD_3	81	97	PF00069	0.398
DOC_PP4_FxxP_1	79	82	PF00568	0.387
DOC_USP7_MATH_1	112	116	PF00917	0.482
DOC_USP7_MATH_1	211	215	PF00917	0.449
DOC_USP7_MATH_1	58	62	PF00917	0.385
DOC_WW_Pin1_4	108	113	PF00397	0.496
DOC_WW_Pin1_4	160	165	PF00397	0.498
DOC_WW_Pin1_4	329	334	PF00397	0.320
DOC_WW_Pin1_4	38	43	PF00397	0.379
LIG_14-3-3_CanoR_1	110	120	PF00244	0.415
LIG_14-3-3_CanoR_1	252	256	PF00244	0.334
LIG_BIR_II_1	1	5	PF00653	0.422
LIG_BRCT_BRCA1_1	213	217	PF00533	0.268
LIG_BRCT_BRCA1_1	40	44	PF00533	0.381
LIG_Clathr_ClatBox_1	20	24	PF01394	0.398
LIG_FHA_1	65	71	PF00498	0.393
LIG_FHA_2	125	131	PF00498	0.448
LIG_FHA_2	29	35	PF00498	0.544
LIG_LIR_Nem_3	41	47	PF02991	0.574
LIG_MLH1_MIPbox_1	40	44	PF16413	0.381
LIG_PCNA_yPIPBox_3	236	244	PF02747	0.376
LIG_PTB_Apo_2	324	331	PF02174	0.357
LIG_PTB_Phospho_1	324	330	PF10480	0.374
LIG_REV1ctd_RIR_1	42	52	PF16727	0.387
LIG_SH2_CRK	321	325	PF00017	0.262
LIG_SH2_CRK	330	334	PF00017	0.365
LIG_SH2_NCK_1	321	325	PF00017	0.262
LIG_SH2_NCK_1	330	334	PF00017	0.365
LIG_SH2_PTP2	194	197	PF00017	0.352
LIG_SH2_SRC	194	197	PF00017	0.380
LIG_SH2_STAP1	262	266	PF00017	0.387
LIG_SH2_STAP1	308	312	PF00017	0.281
LIG_SH2_STAP1	321	325	PF00017	0.277
LIG_SH2_STAT5	13	16	PF00017	0.406
LIG_SH2_STAT5	194	197	PF00017	0.398
LIG_SH2_STAT5	222	225	PF00017	0.271
LIG_SH2_STAT5	308	311	PF00017	0.332
LIG_SH2_STAT5	316	319	PF00017	0.331
LIG_SH2_STAT5	78	81	PF00017	0.608
LIG_SH3_1	193	199	PF00018	0.441
LIG_SH3_2	196	201	PF14604	0.453
LIG_SH3_3	12	18	PF00018	0.390
LIG_SH3_3	19	25	PF00018	0.387
LIG_SH3_3	193	199	PF00018	0.441
LIG_SUMO_SIM_anti_2	277	282	PF11976	0.274
LIG_SUMO_SIM_par_1	174	180	PF11976	0.350
LIG_SUMO_SIM_par_1	18	24	PF11976	0.395
LIG_WRC_WIRS_1	280	285	PF05994	0.283
MOD_CDK_SPK_2	108	113	PF00069	0.413
MOD_CK1_1	124	130	PF00069	0.444
MOD_CK1_1	136	142	PF00069	0.316
MOD_CK1_1	160	166	PF00069	0.459
MOD_CK2_1	124	130	PF00069	0.448
MOD_CK2_1	225	231	PF00069	0.388
MOD_CK2_1	329	335	PF00069	0.390
MOD_CK2_1	341	347	PF00069	0.407
MOD_GlcNHglycan	124	127	PF01048	0.474
MOD_GlcNHglycan	138	141	PF01048	0.268
MOD_GlcNHglycan	158	162	PF01048	0.461
MOD_GlcNHglycan	165	168	PF01048	0.463
MOD_GlcNHglycan	266	269	PF01048	0.233
MOD_GSK3_1	108	115	PF00069	0.443
MOD_GSK3_1	159	166	PF00069	0.361
MOD_GSK3_1	24	31	PF00069	0.422
MOD_GSK3_1	264	271	PF00069	0.474
MOD_GSK3_1	337	344	PF00069	0.455
MOD_GSK3_1	81	88	PF00069	0.437
MOD_LATS_1	225	231	PF00433	0.360
MOD_LATS_1	62	68	PF00433	0.395
MOD_N-GLC_1	122	127	PF02516	0.450
MOD_N-GLC_2	213	215	PF02516	0.280
MOD_NEK2_1	101	106	PF00069	0.472
MOD_NEK2_1	122	127	PF00069	0.341
MOD_NEK2_1	159	164	PF00069	0.345
MOD_NEK2_1	187	192	PF00069	0.533
MOD_NEK2_1	264	269	PF00069	0.264
MOD_NEK2_1	306	311	PF00069	0.352
MOD_NEK2_1	314	319	PF00069	0.341
MOD_NEK2_2	66	71	PF00069	0.408
MOD_OFUCOSY	209	215	PF10250	0.321
MOD_PIKK_1	133	139	PF00454	0.329
MOD_PIKK_1	25	31	PF00454	0.426
MOD_PKA_2	251	257	PF00069	0.372
MOD_PKA_2	6	12	PF00069	0.398
MOD_Plk_1	211	217	PF00069	0.289
MOD_Plk_1	337	343	PF00069	0.453
MOD_Plk_4	130	136	PF00069	0.310
MOD_Plk_4	279	285	PF00069	0.288
MOD_Plk_4	337	343	PF00069	0.447
MOD_Plk_4	66	72	PF00069	0.388
MOD_ProDKin_1	108	114	PF00069	0.502
MOD_ProDKin_1	160	166	PF00069	0.499
MOD_ProDKin_1	329	335	PF00069	0.318
MOD_ProDKin_1	38	44	PF00069	0.379
TRG_DiLeu_BaEn_1	130	135	PF01217	0.528
TRG_DiLeu_BaEn_1	182	187	PF01217	0.398
TRG_DiLeu_BaEn_2	278	284	PF01217	0.415
TRG_DiLeu_BaLyEn_6	15	20	PF01217	0.394
TRG_ENDOCYTIC_2	321	324	PF00928	0.265
TRG_ENDOCYTIC_2	78	81	PF00928	0.397
TRG_ER_diArg_1	107	110	PF00400	0.318
TRG_ER_diArg_1	145	148	PF00400	0.363
TRG_ER_diArg_1	206	208	PF00400	0.354

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P901	Leptomonas seymouri	75%	100%
A0A0S4IPY5	Bodo saltans	47%	100%
A0A1X0NNB5	Trypanosomatidae	52%	100%
A0A3R7LXZ2	Trypanosoma rangeli	55%	100%
A0A3S7XC16	Leishmania donovani	76%	96%
A4IE15	Leishmania infantum	76%	96%
D0A3Y1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	100%
E9AU37	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%
O13974	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	25%	100%
Q05B57	Bos taurus	33%	100%
Q4Q0F8	Leishmania major	75%	100%
Q55BI3	Dictyostelium discoideum	32%	100%
Q5VVQ6	Homo sapiens	34%	100%
Q9VRJ9	Drosophila melanogaster	30%	100%
V5B3J2	Trypanosoma cruzi	55%	100%