tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Gcd10p family, putative

Species:

Leishmania braziliensis

UniProt:

A4HQ94_LEIBR

TriTrypDb:

LbrM.35.6060 , LBRM2903_350072000

Length:

543

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	11
GO:0031515	tRNA (m1A) methyltransferase complex	6	11
GO:0032991	protein-containing complex	1	11
GO:0034708	methyltransferase complex	4	11
GO:0043226	organelle	2	11
GO:0043227	membrane-bounded organelle	3	11
GO:0043229	intracellular organelle	3	11
GO:0043231	intracellular membrane-bounded organelle	4	11
GO:0043527	tRNA methyltransferase complex	5	11
GO:0110165	cellular anatomical entity	1	11
GO:1902494	catalytic complex	2	11
GO:1990234	transferase complex	3	11
GO:0005643	nuclear pore	3	1
GO:0140513	nuclear protein-containing complex	2	1

Expansion

Sequence features

A4HQ94

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HQ94

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006396	RNA processing	6	11
GO:0006399	tRNA metabolic process	7	11
GO:0006400	tRNA modification	6	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008033	tRNA processing	8	11
GO:0008152	metabolic process	1	11
GO:0009451	RNA modification	5	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0030488	tRNA methylation	5	11
GO:0032259	methylation	2	11
GO:0034470	ncRNA processing	7	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034660	ncRNA metabolic process	6	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0043414	macromolecule methylation	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	2
GO:0008168	methyltransferase activity	4	2
GO:0016740	transferase activity	2	2
GO:0016741	transferase activity, transferring one-carbon groups	3	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	155	159	PF00656	0.286
CLV_NRD_NRD_1	180	182	PF00675	0.387
CLV_NRD_NRD_1	21	23	PF00675	0.613
CLV_NRD_NRD_1	37	39	PF00675	0.446
CLV_NRD_NRD_1	8	10	PF00675	0.621
CLV_PCSK_FUR_1	8	12	PF00082	0.490
CLV_PCSK_KEX2_1	134	136	PF00082	0.272
CLV_PCSK_KEX2_1	180	182	PF00082	0.387
CLV_PCSK_KEX2_1	21	23	PF00082	0.613
CLV_PCSK_KEX2_1	462	464	PF00082	0.541
CLV_PCSK_KEX2_1	510	512	PF00082	0.766
CLV_PCSK_KEX2_1	8	10	PF00082	0.621
CLV_PCSK_PC1ET2_1	10	12	PF00082	0.488
CLV_PCSK_PC1ET2_1	134	136	PF00082	0.272
CLV_PCSK_PC1ET2_1	462	464	PF00082	0.541
CLV_PCSK_PC1ET2_1	510	512	PF00082	0.766
CLV_PCSK_SKI1_1	101	105	PF00082	0.387
CLV_PCSK_SKI1_1	209	213	PF00082	0.412
CLV_PCSK_SKI1_1	274	278	PF00082	0.458
CLV_PCSK_SKI1_1	316	320	PF00082	0.507
CLV_PCSK_SKI1_1	387	391	PF00082	0.381
CLV_PCSK_SKI1_1	42	46	PF00082	0.436
CLV_PCSK_SKI1_1	435	439	PF00082	0.451
DEG_APCC_DBOX_1	41	49	PF00400	0.434
DEG_Nend_UBRbox_1	1	4	PF02207	0.503
DEG_SCF_FBW7_1	483	490	PF00400	0.395
DOC_CDC14_PxL_1	48	56	PF14671	0.417
DOC_CKS1_1	32	37	PF01111	0.596
DOC_MAPK_gen_1	100	107	PF00069	0.425
DOC_MAPK_gen_1	254	262	PF00069	0.314
DOC_MAPK_gen_1	38	49	PF00069	0.437
DOC_MAPK_gen_1	8	17	PF00069	0.484
DOC_MAPK_JIP1_4	39	45	PF00069	0.440
DOC_MAPK_MEF2A_6	100	107	PF00069	0.425
DOC_MAPK_MEF2A_6	256	264	PF00069	0.314
DOC_MAPK_MEF2A_6	38	47	PF00069	0.438
DOC_MAPK_MEF2A_6	441	449	PF00069	0.416
DOC_MAPK_MEF2A_6	462	470	PF00069	0.461
DOC_MAPK_NFAT4_5	42	50	PF00069	0.432
DOC_PP2B_LxvP_1	235	238	PF13499	0.458
DOC_PP2B_LxvP_1	318	321	PF13499	0.540
DOC_PP2B_LxvP_1	47	50	PF13499	0.426
DOC_PP2B_PxIxI_1	310	316	PF00149	0.288
DOC_USP7_MATH_1	394	398	PF00917	0.342
DOC_USP7_MATH_1	487	491	PF00917	0.446
DOC_USP7_MATH_1	50	54	PF00917	0.415
DOC_USP7_UBL2_3	100	104	PF12436	0.328
DOC_USP7_UBL2_3	209	213	PF12436	0.312
DOC_USP7_UBL2_3	324	328	PF12436	0.491
DOC_USP7_UBL2_3	523	527	PF12436	0.606
DOC_WW_Pin1_4	31	36	PF00397	0.596
DOC_WW_Pin1_4	402	407	PF00397	0.398
DOC_WW_Pin1_4	424	429	PF00397	0.401
DOC_WW_Pin1_4	483	488	PF00397	0.642
DOC_WW_Pin1_4	495	500	PF00397	0.448
LIG_14-3-3_CanoR_1	226	230	PF00244	0.425
LIG_14-3-3_CanoR_1	408	417	PF00244	0.268
LIG_14-3-3_CanoR_1	485	494	PF00244	0.586
LIG_14-3-3_CanoR_1	73	80	PF00244	0.411
LIG_Clathr_ClatBox_1	221	225	PF01394	0.425
LIG_CtBP_PxDLS_1	430	434	PF00389	0.438
LIG_deltaCOP1_diTrp_1	23	32	PF00928	0.456
LIG_deltaCOP1_diTrp_1	61	70	PF00928	0.424
LIG_eIF4E_1	384	390	PF01652	0.277
LIG_FAT_LD_1	185	193	PF03623	0.458
LIG_FHA_1	114	120	PF00498	0.353
LIG_FHA_1	12	18	PF00498	0.751
LIG_FHA_1	257	263	PF00498	0.344
LIG_FHA_1	279	285	PF00498	0.425
LIG_FHA_1	310	316	PF00498	0.444
LIG_FHA_1	376	382	PF00498	0.546
LIG_FHA_2	189	195	PF00498	0.328
LIG_FHA_2	371	377	PF00498	0.691
LIG_FHA_2	501	507	PF00498	0.607
LIG_LIR_Gen_1	434	445	PF02991	0.386
LIG_LIR_Gen_1	60	71	PF02991	0.423
LIG_LIR_Nem_3	121	126	PF02991	0.401
LIG_LIR_Nem_3	139	145	PF02991	0.293
LIG_LIR_Nem_3	23	27	PF02991	0.457
LIG_LIR_Nem_3	325	330	PF02991	0.365
LIG_LIR_Nem_3	434	440	PF02991	0.383
LIG_LIR_Nem_3	60	66	PF02991	0.416
LIG_LIR_Nem_3	82	88	PF02991	0.359
LIG_LYPXL_yS_3	142	145	PF13949	0.314
LIG_NRBOX	184	190	PF00104	0.425
LIG_Pex14_1	380	384	PF04695	0.295
LIG_SH2_CRK	123	127	PF00017	0.423
LIG_SH2_CRK	218	222	PF00017	0.316
LIG_SH2_PTP2	85	88	PF00017	0.356
LIG_SH2_STAT5	210	213	PF00017	0.298
LIG_SH2_STAT5	236	239	PF00017	0.268
LIG_SH2_STAT5	476	479	PF00017	0.370
LIG_SH2_STAT5	85	88	PF00017	0.335
LIG_SH3_1	328	334	PF00018	0.433
LIG_SH3_3	328	334	PF00018	0.508
LIG_SH3_3	422	428	PF00018	0.381
LIG_SH3_3	493	499	PF00018	0.608
LIG_SUMO_SIM_anti_2	258	264	PF11976	0.314
LIG_SUMO_SIM_par_1	492	498	PF11976	0.481
LIG_TRAF2_1	513	516	PF00917	0.769
LIG_TYR_ITSM	119	126	PF00017	0.206
LIG_UBA3_1	188	195	PF00899	0.387
LIG_UBA3_1	477	482	PF00899	0.390
LIG_UBA3_1	96	104	PF00899	0.206
MOD_CDC14_SPxK_1	405	408	PF00782	0.486
MOD_CDK_SPxK_1	402	408	PF00069	0.498
MOD_CDK_SPxxK_3	31	38	PF00069	0.443
MOD_CK1_1	198	204	PF00069	0.425
MOD_CK1_1	288	294	PF00069	0.458
MOD_CK2_1	169	175	PF00069	0.313
MOD_CK2_1	188	194	PF00069	0.314
MOD_CK2_1	500	506	PF00069	0.594
MOD_CK2_1	509	515	PF00069	0.676
MOD_CK2_1	527	533	PF00069	0.738
MOD_Cter_Amidation	132	135	PF01082	0.328
MOD_GlcNHglycan	109	112	PF01048	0.333
MOD_GlcNHglycan	511	514	PF01048	0.667
MOD_GlcNHglycan	88	91	PF01048	0.425
MOD_GSK3_1	114	121	PF00069	0.209
MOD_GSK3_1	194	201	PF00069	0.235
MOD_GSK3_1	236	243	PF00069	0.317
MOD_GSK3_1	344	351	PF00069	0.726
MOD_GSK3_1	371	378	PF00069	0.548
MOD_GSK3_1	464	471	PF00069	0.462
MOD_GSK3_1	483	490	PF00069	0.444
MOD_GSK3_1	501	508	PF00069	0.709
MOD_GSK3_1	86	93	PF00069	0.361
MOD_N-GLC_1	468	473	PF02516	0.481
MOD_N-GLC_1	74	79	PF02516	0.357
MOD_N-GLC_2	442	444	PF02516	0.328
MOD_NEK2_1	107	112	PF00069	0.314
MOD_NEK2_1	114	119	PF00069	0.308
MOD_NEK2_1	17	22	PF00069	0.691
MOD_NEK2_1	188	193	PF00069	0.283
MOD_NEK2_1	370	375	PF00069	0.735
MOD_NEK2_2	344	349	PF00069	0.488
MOD_PIKK_1	375	381	PF00454	0.452
MOD_PKA_2	225	231	PF00069	0.425
MOD_PKA_2	348	354	PF00069	0.772
MOD_PKB_1	254	262	PF00069	0.314
MOD_PKB_1	31	39	PF00069	0.443
MOD_PKB_1	9	17	PF00069	0.482
MOD_Plk_1	375	381	PF00069	0.406
MOD_Plk_1	505	511	PF00069	0.600
MOD_Plk_2-3	225	231	PF00069	0.419
MOD_Plk_2-3	244	250	PF00069	0.419
MOD_Plk_4	114	120	PF00069	0.425
MOD_Plk_4	188	194	PF00069	0.328
MOD_Plk_4	217	223	PF00069	0.328
MOD_Plk_4	285	291	PF00069	0.458
MOD_Plk_4	394	400	PF00069	0.359
MOD_Plk_4	449	455	PF00069	0.443
MOD_Plk_4	50	56	PF00069	0.413
MOD_ProDKin_1	31	37	PF00069	0.596
MOD_ProDKin_1	402	408	PF00069	0.401
MOD_ProDKin_1	424	430	PF00069	0.405
MOD_ProDKin_1	483	489	PF00069	0.653
MOD_ProDKin_1	495	501	PF00069	0.450
MOD_SUMO_for_1	222	225	PF00179	0.425
TRG_DiLeu_BaEn_1	385	390	PF01217	0.524
TRG_ENDOCYTIC_2	123	126	PF00928	0.415
TRG_ENDOCYTIC_2	142	145	PF00928	0.146
TRG_ENDOCYTIC_2	218	221	PF00928	0.316
TRG_ENDOCYTIC_2	24	27	PF00928	0.462
TRG_ENDOCYTIC_2	300	303	PF00928	0.206
TRG_ENDOCYTIC_2	426	429	PF00928	0.440
TRG_ENDOCYTIC_2	85	88	PF00928	0.335
TRG_ER_diArg_1	179	181	PF00400	0.387
TRG_ER_diArg_1	253	256	PF00400	0.314
TRG_ER_diArg_1	8	11	PF00400	0.622
TRG_NLS_MonoExtC_3	211	216	PF00514	0.314
TRG_NLS_MonoExtN_4	209	216	PF00514	0.314
TRG_Pf-PMV_PEXEL_1	160	164	PF00026	0.314
TRG_Pf-PMV_PEXEL_1	183	187	PF00026	0.403
TRG_Pf-PMV_PEXEL_1	387	392	PF00026	0.435

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I267	Leptomonas seymouri	73%	100%
A0A1X0NLQ1	Trypanosomatidae	53%	100%
A0A3Q8IJV9	Leishmania donovani	82%	100%
A0A422NSE7	Trypanosoma rangeli	53%	100%
A4IDZ2	Leishmania infantum	82%	100%
D0A417	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	100%
E9AU12	Leishmania mexicana (strain MHOM/GT/2001/U1103)	81%	100%
P41814	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	22%	100%
Q2T9V5	Bos taurus	25%	100%
Q4Q0I3	Leishmania major	80%	100%
Q54UB1	Dictyostelium discoideum	28%	100%
Q5A6Q4	Candida albicans (strain SC5314 / ATCC MYA-2876)	24%	100%
Q6BKK7	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	23%	100%
Q6CF35	Yarrowia lipolytica (strain CLIB 122 / E 150)	22%	100%
Q6CLF6	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	22%	100%
Q6FNP9	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	22%	100%
Q75B32	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	22%	100%
Q8CE96	Mus musculus	26%	100%
Q9HGL4	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	25%	100%
Q9UJA5	Homo sapiens	25%	100%
V5B3L2	Trypanosoma cruzi	52%	100%