GOLD domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

emp24/gp25L/p24 family/GOLD, putative

Species:

Leishmania braziliensis

UniProt:

A4HQ73_LEIBR

TriTrypDb:

LbrM.35.5840 , LBRM2903_350069800

Length:

241

Annotations

LeishMANIAdb annotations

Conserved eukaryotic protein. Probable receptor for ER-Golgi trafficking of GPI-anchored proteins. distinct from the other GOLD domain cluster (related to YB79_SCHPO instead). Localization: ER (by homology) / Golgi (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 8, no: 1
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	10
GO:0110165	cellular anatomical entity	1	10
GO:0005783	endoplasmic reticulum	5	1
GO:0005793	endoplasmic reticulum-Golgi intermediate compartment	5	1
GO:0005794	Golgi apparatus	5	1
GO:0030134	COPII-coated ER to Golgi transport vesicle	8	1
GO:0030135	coated vesicle	7	1
GO:0031410	cytoplasmic vesicle	6	1
GO:0031982	vesicle	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0097708	intracellular vesicle	5	1

Expansion

Sequence features

A4HQ73

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HQ73

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	1
GO:0006886	intracellular protein transport	4	1
GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport	4	1
GO:0006996	organelle organization	4	1
GO:0007030	Golgi organization	5	1
GO:0008104	protein localization	4	1
GO:0009987	cellular process	1	1
GO:0015031	protein transport	4	1
GO:0016043	cellular component organization	3	1
GO:0016192	vesicle-mediated transport	4	1
GO:0033036	macromolecule localization	2	1
GO:0045184	establishment of protein localization	3	1
GO:0046907	intracellular transport	3	1
GO:0048193	Golgi vesicle transport	5	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0051641	cellular localization	2	1
GO:0051649	establishment of localization in cell	3	1
GO:0070727	cellular macromolecule localization	3	1
GO:0071702	organic substance transport	4	1
GO:0071705	nitrogen compound transport	4	1
GO:0071840	cellular component organization or biogenesis	2	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	194	196	PF00675	0.530
CLV_PCSK_KEX2_1	194	196	PF00082	0.530
CLV_PCSK_KEX2_1	93	95	PF00082	0.622
CLV_PCSK_PC1ET2_1	93	95	PF00082	0.622
CLV_PCSK_PC7_1	89	95	PF00082	0.497
CLV_PCSK_SKI1_1	195	199	PF00082	0.530
DEG_Nend_Nbox_1	1	3	PF02207	0.407
DOC_CYCLIN_RxL_1	172	183	PF00134	0.302
DOC_MAPK_gen_1	132	138	PF00069	0.302
DOC_MAPK_gen_1	173	181	PF00069	0.296
DOC_MAPK_gen_1	93	100	PF00069	0.418
DOC_MAPK_MEF2A_6	12	21	PF00069	0.517
DOC_MAPK_MEF2A_6	173	181	PF00069	0.247
DOC_MAPK_MEF2A_6	93	100	PF00069	0.390
DOC_PP1_RVXF_1	122	128	PF00149	0.207
DOC_PP2B_LxvP_1	84	87	PF13499	0.244
DOC_PP2B_LxvP_1	96	99	PF13499	0.301
DOC_PP4_FxxP_1	127	130	PF00568	0.330
LIG_Actin_WH2_2	77	95	PF00022	0.374
LIG_BRCT_BRCA1_1	110	114	PF00533	0.240
LIG_eIF4E_1	123	129	PF01652	0.330
LIG_FHA_1	120	126	PF00498	0.263
LIG_FHA_2	104	110	PF00498	0.406
LIG_FHA_2	181	187	PF00498	0.267
LIG_FHA_2	25	31	PF00498	0.479
LIG_GBD_Chelix_1	17	25	PF00786	0.486
LIG_LIR_Gen_1	139	149	PF02991	0.244
LIG_LIR_Gen_1	204	215	PF02991	0.306
LIG_LIR_Nem_3	105	110	PF02991	0.353
LIG_LIR_Nem_3	139	145	PF02991	0.244
LIG_LIR_Nem_3	204	210	PF02991	0.264
LIG_LIR_Nem_3	52	56	PF02991	0.389
LIG_PCNA_APIM_2	231	237	PF02747	0.502
LIG_Pex14_1	226	230	PF04695	0.447
LIG_Pex14_2	138	142	PF04695	0.247
LIG_SH2_PTP2	40	43	PF00017	0.248
LIG_SH2_SRC	85	88	PF00017	0.323
LIG_SH2_STAP1	170	174	PF00017	0.290
LIG_SH2_STAT5	196	199	PF00017	0.309
LIG_SH2_STAT5	207	210	PF00017	0.302
LIG_SH2_STAT5	230	233	PF00017	0.447
LIG_SH2_STAT5	234	237	PF00017	0.541
LIG_SH2_STAT5	40	43	PF00017	0.242
LIG_SH3_3	146	152	PF00018	0.330
LIG_SH3_3	158	164	PF00018	0.234
LIG_SUMO_SIM_par_1	61	67	PF11976	0.263
LIG_UBA3_1	128	133	PF00899	0.330
LIG_WRC_WIRS_1	104	109	PF05994	0.346
LIG_WRC_WIRS_1	65	70	PF05994	0.235
MOD_CK1_1	180	186	PF00069	0.330
MOD_CK2_1	181	187	PF00069	0.265
MOD_CK2_1	24	30	PF00069	0.447
MOD_GlcNHglycan	142	145	PF01048	0.532
MOD_GlcNHglycan	203	206	PF01048	0.552
MOD_GlcNHglycan	4	7	PF01048	0.579
MOD_GSK3_1	177	184	PF00069	0.330
MOD_N-GLC_1	177	182	PF02516	0.530
MOD_NEK2_1	1	6	PF00069	0.578
MOD_NEK2_1	138	143	PF00069	0.330
MOD_NEK2_1	190	195	PF00069	0.354
MOD_NEK2_1	198	203	PF00069	0.314
MOD_NEK2_2	31	36	PF00069	0.568
MOD_PIKK_1	108	114	PF00454	0.330
MOD_Plk_1	108	114	PF00069	0.330
MOD_Plk_1	138	144	PF00069	0.330
MOD_Plk_1	177	183	PF00069	0.330
MOD_Plk_1	198	204	PF00069	0.333
MOD_Plk_4	221	227	PF00069	0.453
MOD_SUMO_for_1	152	155	PF00179	0.302
TRG_DiLeu_BaEn_2	154	160	PF01217	0.330
TRG_ENDOCYTIC_2	207	210	PF00928	0.308
TRG_ENDOCYTIC_2	40	43	PF00928	0.327
TRG_ER_diArg_1	173	176	PF00400	0.338
TRG_ER_diArg_1	194	196	PF00400	0.330

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IAH0	Leptomonas seymouri	75%	100%
A0A1X0P077	Trypanosomatidae	45%	100%
A0A3Q8IJ75	Leishmania donovani	83%	100%
A0A422NKF6	Trypanosoma rangeli	45%	100%
A4IDX1	Leishmania infantum	83%	100%
E9ATZ2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	82%	100%
Q4Q0K4	Leishmania major	85%	100%
V5BTC3	Trypanosoma cruzi	46%	100%