Vacuolar protein sorting-associated protein 28 homolog

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

vacuolar protein sorting-associated protein-like protein

Species:

Leishmania braziliensis

UniProt:

A4HQ33_LEIBR

TriTrypDb:

LbrM.35.5420 , LBRM2903_350065200

Length:

209

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 12

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0000813	ESCRT I complex	3	11
GO:0032991	protein-containing complex	1	11
GO:0036452	ESCRT complex	2	11
GO:0098796	membrane protein complex	2	11

Expansion

Sequence features

A4HQ33

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HQ33

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	11
GO:0006886	intracellular protein transport	4	11
GO:0007034	vacuolar transport	4	11
GO:0008104	protein localization	4	11
GO:0009987	cellular process	1	11
GO:0015031	protein transport	4	11
GO:0016192	vesicle-mediated transport	4	11
GO:0016197	endosomal transport	4	11
GO:0032509	endosome transport via multivesicular body sorting pathway	5	11
GO:0032511	late endosome to vacuole transport via multivesicular body sorting pathway	6	11
GO:0033036	macromolecule localization	2	11
GO:0033365	protein localization to organelle	5	11
GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	5	11
GO:0045184	establishment of protein localization	3	11
GO:0045324	late endosome to vacuole transport	5	11
GO:0046907	intracellular transport	3	11
GO:0051179	localization	1	11
GO:0051234	establishment of localization	2	11
GO:0051641	cellular localization	2	11
GO:0051649	establishment of localization in cell	3	11
GO:0070727	cellular macromolecule localization	3	11
GO:0071702	organic substance transport	4	11
GO:0071705	nitrogen compound transport	4	11
GO:0071985	multivesicular body sorting pathway	5	11
GO:0072594	establishment of protein localization to organelle	4	11
GO:0072665	protein localization to vacuole	6	11
GO:0072666	establishment of protein localization to vacuole	5	11

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	1
GO:0044877	protein-containing complex binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	120	124	PF00656	0.306
CLV_C14_Caspase3-7	90	94	PF00656	0.415
CLV_NRD_NRD_1	146	148	PF00675	0.435
CLV_NRD_NRD_1	165	167	PF00675	0.398
CLV_NRD_NRD_1	35	37	PF00675	0.437
CLV_NRD_NRD_1	49	51	PF00675	0.338
CLV_PCSK_KEX2_1	165	167	PF00082	0.409
CLV_PCSK_KEX2_1	35	37	PF00082	0.453
CLV_PCSK_KEX2_1	49	51	PF00082	0.338
CLV_PCSK_SKI1_1	196	200	PF00082	0.360
CLV_PCSK_SKI1_1	49	53	PF00082	0.352
DOC_CYCLIN_RxL_1	144	155	PF00134	0.360
DOC_CYCLIN_RxL_1	46	53	PF00134	0.338
DOC_PP2B_LxvP_1	135	138	PF13499	0.428
DOC_USP7_MATH_1	108	112	PF00917	0.446
DOC_WW_Pin1_4	4	9	PF00397	0.423
DOC_WW_Pin1_4	95	100	PF00397	0.402
LIG_14-3-3_CanoR_1	109	113	PF00244	0.428
LIG_14-3-3_CanoR_1	126	135	PF00244	0.428
LIG_BIR_II_1	1	5	PF00653	0.715
LIG_BRCT_BRCA1_1	114	118	PF00533	0.428
LIG_FHA_2	118	124	PF00498	0.317
LIG_FHA_2	128	134	PF00498	0.295
LIG_FHA_2	69	75	PF00498	0.351
LIG_FHA_2	88	94	PF00498	0.393
LIG_IBAR_NPY_1	67	69	PF08397	0.313
LIG_LIR_Gen_1	115	125	PF02991	0.442
LIG_LIR_Gen_1	189	199	PF02991	0.336
LIG_LIR_Gen_1	19	27	PF02991	0.322
LIG_LIR_Nem_3	115	121	PF02991	0.428
LIG_LIR_Nem_3	189	195	PF02991	0.336
LIG_LIR_Nem_3	19	24	PF02991	0.314
LIG_LIR_Nem_3	74	80	PF02991	0.343
LIG_NRP_CendR_1	208	209	PF00754	0.707
LIG_PCNA_yPIPBox_3	136	148	PF02747	0.338
LIG_PTB_Apo_2	64	71	PF02174	0.295
LIG_REV1ctd_RIR_1	74	83	PF16727	0.428
LIG_SH2_CRK	192	196	PF00017	0.241
LIG_SH2_STAP1	44	48	PF00017	0.295
LIG_SH2_STAT5	16	19	PF00017	0.312
LIG_SH2_STAT5	69	72	PF00017	0.330
LIG_SH2_STAT5	80	83	PF00017	0.362
LIG_SH3_3	131	137	PF00018	0.428
LIG_SUMO_SIM_anti_2	130	136	PF11976	0.428
LIG_SUMO_SIM_par_1	23	28	PF11976	0.341
LIG_UBA3_1	142	148	PF00899	0.351
LIG_UBA3_1	24	32	PF00899	0.319
MOD_CDK_SPxxK_3	4	11	PF00069	0.406
MOD_CK1_1	201	207	PF00069	0.560
MOD_CK2_1	95	101	PF00069	0.402
MOD_GlcNHglycan	63	66	PF01048	0.308
MOD_GSK3_1	108	115	PF00069	0.361
MOD_GSK3_1	117	124	PF00069	0.263
MOD_NEK2_1	121	126	PF00069	0.343
MOD_NEK2_1	127	132	PF00069	0.343
MOD_NEK2_1	176	181	PF00069	0.502
MOD_NEK2_2	44	49	PF00069	0.313
MOD_NEK2_2	87	92	PF00069	0.428
MOD_PKA_2	108	114	PF00069	0.428
MOD_ProDKin_1	4	10	PF00069	0.417
MOD_ProDKin_1	95	101	PF00069	0.402
TRG_DiLeu_BaLyEn_6	47	52	PF01217	0.295
TRG_ENDOCYTIC_2	192	195	PF00928	0.295
TRG_ER_diArg_1	165	167	PF00400	0.424
TRG_ER_diArg_1	48	50	PF00400	0.338
TRG_NES_CRM1_1	141	155	PF08389	0.338
TRG_NES_CRM1_1	15	28	PF08389	0.295
TRG_Pf-PMV_PEXEL_1	105	110	PF00026	0.430
TRG_Pf-PMV_PEXEL_1	36	40	PF00026	0.291
TRG_Pf-PMV_PEXEL_1	49	53	PF00026	0.338

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HW69	Leptomonas seymouri	89%	100%
A0A0S4IXL0	Bodo saltans	32%	100%
A0A1X0P3A4	Trypanosomatidae	50%	97%
A0A3Q8IWE4	Leishmania donovani	91%	100%
A0A3R7MQI1	Trypanosoma rangeli	50%	99%
A4IDT9	Leishmania infantum	91%	100%
A8XWB7	Caenorhabditis briggsae	33%	100%
B5DEN9	Rattus norvegicus	31%	92%
D0A8T3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	49%	96%
E9ATV1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
O13872	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	84%
O65421	Arabidopsis thaliana	32%	100%
Q02767	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	29%	86%
Q3T178	Bos taurus	31%	95%
Q4Q0P5	Leishmania major	89%	100%
Q54NF1	Dictyostelium discoideum	28%	73%
Q9D1C8	Mus musculus	31%	95%
Q9NA26	Caenorhabditis elegans	33%	100%
Q9S9T7	Arabidopsis thaliana	34%	100%
Q9UK41	Homo sapiens	31%	95%
Q9V359	Drosophila melanogaster	36%	100%
V5BRX4	Trypanosoma cruzi	52%	98%