Glycine--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

glycyl tRNA synthetase, putative

Species:

Leishmania braziliensis

UniProt:

A4HPQ1_LEIBR

TriTrypDb:

LbrM.35.4070 , LBRM2903_350050900

Length:

627

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 2
Pissara et al.	yes	yes: 8

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 4

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 4

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	12
GO:0110165	cellular anatomical entity	1	12
GO:0005739	mitochondrion	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

A4HPQ1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HPQ1

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006399	tRNA metabolic process	7	12
GO:0006418	tRNA aminoacylation for protein translation	6	12
GO:0006426	glycyl-tRNA aminoacylation	7	12
GO:0006520	amino acid metabolic process	3	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0019752	carboxylic acid metabolic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043038	amino acid activation	4	12
GO:0043039	tRNA aminoacylation	5	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043436	oxoacid metabolic process	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044281	small molecule metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:0000959	mitochondrial RNA metabolic process	6	1
GO:0070127	tRNA aminoacylation for mitochondrial protein translation	7	1
GO:0070150	mitochondrial glycyl-tRNA aminoacylation	8	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004812	aminoacyl-tRNA ligase activity	4	12
GO:0004820	glycine-tRNA ligase activity	5	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	288	292	PF00656	0.510
CLV_NRD_NRD_1	24	26	PF00675	0.493
CLV_NRD_NRD_1	489	491	PF00675	0.375
CLV_PCSK_KEX2_1	202	204	PF00082	0.303
CLV_PCSK_KEX2_1	23	25	PF00082	0.498
CLV_PCSK_KEX2_1	402	404	PF00082	0.297
CLV_PCSK_KEX2_1	489	491	PF00082	0.340
CLV_PCSK_KEX2_1	498	500	PF00082	0.332
CLV_PCSK_PC1ET2_1	202	204	PF00082	0.303
CLV_PCSK_PC1ET2_1	402	404	PF00082	0.297
CLV_PCSK_PC1ET2_1	498	500	PF00082	0.320
CLV_PCSK_SKI1_1	231	235	PF00082	0.310
CLV_PCSK_SKI1_1	238	242	PF00082	0.266
CLV_PCSK_SKI1_1	24	28	PF00082	0.494
CLV_PCSK_SKI1_1	306	310	PF00082	0.280
CLV_PCSK_SKI1_1	385	389	PF00082	0.411
CLV_PCSK_SKI1_1	499	503	PF00082	0.300
CLV_PCSK_SKI1_1	512	516	PF00082	0.254
DEG_APCC_DBOX_1	230	238	PF00400	0.497
DEG_APCC_DBOX_1	384	392	PF00400	0.626
DEG_ODPH_VHL_1	508	520	PF01847	0.454
DOC_CYCLIN_RxL_1	382	392	PF00134	0.630
DOC_CYCLIN_yCln2_LP_2	561	567	PF00134	0.444
DOC_CYCLIN_yCln2_LP_2	602	608	PF00134	0.575
DOC_MAPK_DCC_7	512	520	PF00069	0.444
DOC_MAPK_gen_1	23	32	PF00069	0.288
DOC_MAPK_gen_1	553	563	PF00069	0.444
DOC_MAPK_JIP1_4	521	527	PF00069	0.463
DOC_MAPK_MEF2A_6	512	520	PF00069	0.467
DOC_MAPK_MEF2A_6	557	565	PF00069	0.444
DOC_PP2B_LxvP_1	507	510	PF13499	0.481
DOC_PP2B_LxvP_1	561	564	PF13499	0.444
DOC_PP4_FxxP_1	165	168	PF00568	0.357
DOC_PP4_FxxP_1	248	251	PF00568	0.491
DOC_USP7_MATH_1	354	358	PF00917	0.526
DOC_USP7_MATH_1	50	54	PF00917	0.382
DOC_USP7_UBL2_3	254	258	PF12436	0.511
DOC_USP7_UBL2_3	398	402	PF12436	0.546
DOC_WW_Pin1_4	156	161	PF00397	0.313
DOC_WW_Pin1_4	461	466	PF00397	0.622
DOC_WW_Pin1_4	502	507	PF00397	0.552
DOC_WW_Pin1_4	75	80	PF00397	0.301
LIG_14-3-3_CanoR_1	322	332	PF00244	0.634
LIG_14-3-3_CanoR_1	499	505	PF00244	0.496
LIG_14-3-3_CanoR_1	538	543	PF00244	0.548
LIG_14-3-3_CanoR_1	589	599	PF00244	0.446
LIG_AP2alpha_1	165	169	PF02296	0.357
LIG_AP2alpha_2	167	169	PF02296	0.423
LIG_APCC_ABBA_1	307	312	PF00400	0.481
LIG_APCC_ABBAyCdc20_2	306	312	PF00400	0.427
LIG_BIR_II_1	1	5	PF00653	0.467
LIG_BIR_III_4	139	143	PF00653	0.436
LIG_BRCT_BRCA1_1	165	169	PF00533	0.275
LIG_deltaCOP1_diTrp_1	266	270	PF00928	0.490
LIG_EH1_1	471	479	PF00400	0.469
LIG_FHA_1	36	42	PF00498	0.361
LIG_FHA_1	390	396	PF00498	0.611
LIG_FHA_1	431	437	PF00498	0.580
LIG_FHA_1	462	468	PF00498	0.586
LIG_FHA_1	594	600	PF00498	0.463
LIG_FHA_1	620	626	PF00498	0.538
LIG_FHA_1	72	78	PF00498	0.329
LIG_FHA_2	162	168	PF00498	0.211
LIG_FHA_2	239	245	PF00498	0.471
LIG_FHA_2	576	582	PF00498	0.444
LIG_FHA_2	76	82	PF00498	0.299
LIG_LIR_Apic_2	164	168	PF02991	0.203
LIG_LIR_Apic_2	246	251	PF02991	0.486
LIG_LIR_Apic_2	266	271	PF02991	0.400
LIG_LIR_Apic_2	376	381	PF02991	0.540
LIG_LIR_Gen_1	131	141	PF02991	0.435
LIG_LIR_Gen_1	257	267	PF02991	0.524
LIG_LIR_Gen_1	610	619	PF02991	0.521
LIG_LIR_Gen_1	90	97	PF02991	0.357
LIG_LIR_Nem_3	131	136	PF02991	0.389
LIG_LIR_Nem_3	257	262	PF02991	0.518
LIG_LIR_Nem_3	343	348	PF02991	0.480
LIG_LIR_Nem_3	503	507	PF02991	0.587
LIG_LIR_Nem_3	610	614	PF02991	0.500
LIG_LIR_Nem_3	90	95	PF02991	0.370
LIG_MYND_1	506	510	PF01753	0.559
LIG_NRBOX	477	483	PF00104	0.461
LIG_Pex14_1	59	63	PF04695	0.268
LIG_Pex14_2	111	115	PF04695	0.401
LIG_Pex14_2	165	169	PF04695	0.290
LIG_Pex14_2	171	175	PF04695	0.491
LIG_SH2_GRB2like	410	413	PF00017	0.542
LIG_SH2_NCK_1	555	559	PF00017	0.444
LIG_SH2_SRC	378	381	PF00017	0.621
LIG_SH2_STAT3	618	621	PF00017	0.616
LIG_SH2_STAT5	302	305	PF00017	0.449
LIG_SH2_STAT5	410	413	PF00017	0.566
LIG_SH2_STAT5	463	466	PF00017	0.501
LIG_SH2_STAT5	479	482	PF00017	0.430
LIG_SH3_3	464	470	PF00018	0.508
LIG_SH3_3	585	591	PF00018	0.444
LIG_SH3_3	602	608	PF00018	0.508
LIG_SUMO_SIM_anti_2	523	530	PF11976	0.506
LIG_SUMO_SIM_par_1	432	438	PF11976	0.573
LIG_SUMO_SIM_par_1	523	530	PF11976	0.470
LIG_TRAF2_1	374	377	PF00917	0.531
LIG_TRAF2_1	438	441	PF00917	0.652
LIG_TRAF2_1	450	453	PF00917	0.623
LIG_TRAF2_1	78	81	PF00917	0.323
LIG_TRFH_1	42	46	PF08558	0.335
LIG_TYR_ITIM	477	482	PF00017	0.450
LIG_TYR_ITSM	341	348	PF00017	0.473
LIG_UBA3_1	387	393	PF00899	0.627
LIG_UBA3_1	515	521	PF00899	0.510
LIG_WRC_WIRS_1	448	453	PF05994	0.612
LIG_WRC_WIRS_1	501	506	PF05994	0.586
MOD_CDK_SPK_2	156	161	PF00069	0.275
MOD_CK1_1	2	8	PF00069	0.348
MOD_CK1_1	365	371	PF00069	0.535
MOD_CK1_1	547	553	PF00069	0.462
MOD_CK1_1	593	599	PF00069	0.440
MOD_CK2_1	13	19	PF00069	0.334
MOD_CK2_1	238	244	PF00069	0.473
MOD_CK2_1	435	441	PF00069	0.610
MOD_CK2_1	447	453	PF00069	0.579
MOD_CK2_1	463	469	PF00069	0.472
MOD_CK2_1	575	581	PF00069	0.444
MOD_CK2_1	65	71	PF00069	0.314
MOD_CK2_1	75	81	PF00069	0.284
MOD_Cter_Amidation	400	403	PF01082	0.424
MOD_Cter_Amidation	551	554	PF01082	0.244
MOD_GlcNHglycan	1	4	PF01048	0.708
MOD_GlcNHglycan	206	209	PF01048	0.346
MOD_GlcNHglycan	231	234	PF01048	0.342
MOD_GlcNHglycan	355	359	PF01048	0.291
MOD_GlcNHglycan	437	440	PF01048	0.429
MOD_GlcNHglycan	85	88	PF01048	0.489
MOD_GSK3_1	459	466	PF00069	0.561
MOD_GSK3_1	590	597	PF00069	0.609
MOD_GSK3_1	71	78	PF00069	0.304
MOD_GSK3_1	83	90	PF00069	0.303
MOD_LATS_1	256	262	PF00433	0.524
MOD_N-GLC_1	252	257	PF02516	0.342
MOD_N-GLC_1	296	301	PF02516	0.267
MOD_N-GLC_1	365	370	PF02516	0.400
MOD_NEK2_1	174	179	PF00069	0.509
MOD_NEK2_1	18	23	PF00069	0.297
MOD_NEK2_1	204	209	PF00069	0.518
MOD_NEK2_1	310	315	PF00069	0.519
MOD_NEK2_1	341	346	PF00069	0.489
MOD_NEK2_1	482	487	PF00069	0.464
MOD_NEK2_1	65	70	PF00069	0.281
MOD_NEK2_1	83	88	PF00069	0.365
MOD_NEK2_2	575	580	PF00069	0.444
MOD_OFUCOSY	70	76	PF10250	0.511
MOD_PIKK_1	359	365	PF00454	0.508
MOD_PIKK_1	583	589	PF00454	0.548
MOD_PK_1	258	264	PF00069	0.522
MOD_PK_1	538	544	PF00069	0.548
MOD_PKA_2	456	462	PF00069	0.461
MOD_PKA_2	581	587	PF00069	0.444
MOD_PKA_2	97	103	PF00069	0.317
MOD_PKB_1	236	244	PF00069	0.478
MOD_Plk_1	238	244	PF00069	0.473
MOD_Plk_1	296	302	PF00069	0.465
MOD_Plk_1	354	360	PF00069	0.488
MOD_Plk_1	440	446	PF00069	0.617
MOD_Plk_1	482	488	PF00069	0.467
MOD_Plk_1	544	550	PF00069	0.480
MOD_Plk_1	593	599	PF00069	0.556
MOD_Plk_1	65	71	PF00069	0.318
MOD_Plk_1	72	78	PF00069	0.316
MOD_Plk_4	463	469	PF00069	0.472
MOD_Plk_4	482	488	PF00069	0.467
MOD_Plk_4	594	600	PF00069	0.583
MOD_ProDKin_1	156	162	PF00069	0.313
MOD_ProDKin_1	461	467	PF00069	0.617
MOD_ProDKin_1	502	508	PF00069	0.548
MOD_ProDKin_1	75	81	PF00069	0.304
MOD_SUMO_for_1	520	523	PF00179	0.444
MOD_SUMO_for_1	8	11	PF00179	0.354
MOD_SUMO_rev_2	288	295	PF00179	0.579
MOD_SUMO_rev_2	450	456	PF00179	0.545
TRG_DiLeu_BaEn_1	132	137	PF01217	0.383
TRG_DiLeu_BaEn_2	243	249	PF01217	0.471
TRG_DiLeu_BaLyEn_6	503	508	PF01217	0.502
TRG_ENDOCYTIC_2	345	348	PF00928	0.485
TRG_ENDOCYTIC_2	479	482	PF00928	0.456
TRG_ENDOCYTIC_2	555	558	PF00928	0.444
TRG_ER_diArg_1	23	25	PF00400	0.298
TRG_ER_diArg_1	235	238	PF00400	0.487
TRG_ER_diArg_1	488	490	PF00400	0.513
TRG_NES_CRM1_1	298	312	PF08389	0.477
TRG_Pf-PMV_PEXEL_1	110	114	PF00026	0.512
TRG_Pf-PMV_PEXEL_1	385	390	PF00026	0.364

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HZB0	Leptomonas seymouri	84%	95%
A0A0S4JJA5	Bodo saltans	66%	100%
A0A1X0P3R2	Trypanosomatidae	73%	100%
A0A3Q8IPA6	Leishmania donovani	94%	100%
A0A422MT81	Trypanosoma rangeli	70%	100%
A0B5U4	Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT)	38%	100%
A4FZX1	Methanococcus maripaludis (strain C5 / ATCC BAA-1333)	37%	100%
A4ID12	Leishmania infantum	94%	100%
A6URK3	Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB)	37%	100%
A6VIK1	Methanococcus maripaludis (strain C7 / ATCC BAA-1331)	38%	100%
A9A885	Methanococcus maripaludis (strain C6 / ATCC BAA-1332)	38%	100%
C5A1H0	Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)	37%	100%
C6A317	Thermococcus sibiricus (strain DSM 12597 / MM 739)	36%	100%
D0A8G2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	68%	100%
E9ATG6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
O23627	Arabidopsis thaliana	47%	86%
O27874	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	39%	100%
O29346	Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)	39%	100%
O59235	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	36%	100%
P38088	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	44%	91%
P41250	Homo sapiens	45%	85%
Q04451	Bombyx mori	44%	86%
Q06817	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	42%	100%
Q10039	Caenorhabditis elegans	44%	85%
Q10179	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	42%	96%
Q4Q131	Leishmania major	93%	100%
Q57681	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	39%	100%
Q5I0G4	Rattus norvegicus	46%	86%
Q5JID8	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	37%	100%
Q5RBL1	Pongo abelii	45%	85%
Q6M0Q7	Methanococcus maripaludis (strain S2 / LL)	37%	100%
Q8SQZ6	Encephalitozoon cuniculi (strain GB-M1)	41%	100%
Q8TYY9	Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)	37%	100%
Q8U0G2	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	36%	100%
Q9CZD3	Mus musculus	46%	86%
Q9RSR5	Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)	30%	100%
Q9V176	Pyrococcus abyssi (strain GE5 / Orsay)	36%	100%
Q9VUK8	Drosophila melanogaster	45%	82%
Q9YBF8	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	35%	100%
V5AU76	Trypanosoma cruzi	72%	100%