EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania braziliensis

UniProt:

A4HPH8_LEIBR

TriTrypDb:

LbrM.35.3340 , LBRM2903_350042200

Length:

568

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	1
GO:0031514	motile cilium	5	1
GO:0042995	cell projection	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0110165	cellular anatomical entity	1	2
GO:0120025	plasma membrane bounded cell projection	3	1
GO:0016020	membrane	2	1

Expansion

Sequence features

A4HPH8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HPH8

Function

Biological processes
Term	Name	Level	Count
GO:0006996	organelle organization	4	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0030030	cell projection organization	4	1
GO:0030031	cell projection assembly	5	1
GO:0044782	cilium organization	5	1
GO:0060271	cilium assembly	6	1
GO:0070925	organelle assembly	5	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:0120031	plasma membrane bounded cell projection assembly	6	1
GO:0120036	plasma membrane bounded cell projection organization	5	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	4
GO:0005509	calcium ion binding	5	4
GO:0043167	ion binding	2	4
GO:0043169	cation binding	3	4
GO:0046872	metal ion binding	4	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	382	386	PF00656	0.475
CLV_C14_Caspase3-7	442	446	PF00656	0.446
CLV_NRD_NRD_1	349	351	PF00675	0.497
CLV_NRD_NRD_1	358	360	PF00675	0.436
CLV_NRD_NRD_1	548	550	PF00675	0.469
CLV_PCSK_FUR_1	347	351	PF00082	0.472
CLV_PCSK_KEX2_1	349	351	PF00082	0.463
CLV_PCSK_KEX2_1	358	360	PF00082	0.411
CLV_PCSK_KEX2_1	378	380	PF00082	0.284
CLV_PCSK_KEX2_1	548	550	PF00082	0.494
CLV_PCSK_PC1ET2_1	378	380	PF00082	0.340
CLV_PCSK_PC7_1	354	360	PF00082	0.266
CLV_PCSK_PC7_1	544	550	PF00082	0.461
CLV_PCSK_SKI1_1	349	353	PF00082	0.477
CLV_PCSK_SKI1_1	354	358	PF00082	0.446
CLV_PCSK_SKI1_1	496	500	PF00082	0.371
CLV_PCSK_SKI1_1	50	54	PF00082	0.563
CLV_PCSK_SKI1_1	517	521	PF00082	0.416
CLV_PCSK_SKI1_1	548	552	PF00082	0.417
CLV_PCSK_SKI1_1	557	561	PF00082	0.419
CLV_PCSK_SKI1_1	58	62	PF00082	0.388
DEG_SPOP_SBC_1	290	294	PF00917	0.743
DOC_CYCLIN_RxL_1	493	501	PF00134	0.242
DOC_MAPK_gen_1	262	269	PF00069	0.547
DOC_MAPK_gen_1	318	326	PF00069	0.359
DOC_MAPK_gen_1	358	366	PF00069	0.505
DOC_MAPK_HePTP_8	88	100	PF00069	0.404
DOC_MAPK_MEF2A_6	13	20	PF00069	0.374
DOC_MAPK_MEF2A_6	333	341	PF00069	0.473
DOC_MAPK_MEF2A_6	359	368	PF00069	0.494
DOC_MAPK_MEF2A_6	398	406	PF00069	0.260
DOC_MAPK_MEF2A_6	91	100	PF00069	0.544
DOC_MAPK_RevD_3	364	379	PF00069	0.314
DOC_PP1_MyPhoNE_1	30	37	PF00149	0.556
DOC_PP1_RVXF_1	327	333	PF00149	0.353
DOC_PP4_FxxP_1	213	216	PF00568	0.462
DOC_USP7_MATH_1	487	491	PF00917	0.451
DOC_USP7_MATH_1	505	509	PF00917	0.501
DOC_WW_Pin1_4	12	17	PF00397	0.443
DOC_WW_Pin1_4	212	217	PF00397	0.408
DOC_WW_Pin1_4	366	371	PF00397	0.407
DOC_WW_Pin1_4	482	487	PF00397	0.339
LIG_14-3-3_CanoR_1	126	132	PF00244	0.372
LIG_14-3-3_CanoR_1	180	188	PF00244	0.459
LIG_14-3-3_CanoR_1	299	306	PF00244	0.637
LIG_14-3-3_CanoR_1	517	522	PF00244	0.436
LIG_Actin_WH2_2	164	182	PF00022	0.441
LIG_Actin_WH2_2	543	559	PF00022	0.462
LIG_BIR_II_1	1	5	PF00653	0.576
LIG_BIR_III_2	282	286	PF00653	0.437
LIG_BRCT_BRCA1_1	422	426	PF00533	0.449
LIG_eIF4E_1	14	20	PF01652	0.420
LIG_FHA_1	126	132	PF00498	0.383
LIG_FHA_1	299	305	PF00498	0.640
LIG_FHA_1	371	377	PF00498	0.500
LIG_FHA_1	398	404	PF00498	0.440
LIG_FHA_1	469	475	PF00498	0.410
LIG_FHA_1	51	57	PF00498	0.536
LIG_FHA_1	526	532	PF00498	0.456
LIG_FHA_1	67	73	PF00498	0.535
LIG_FHA_2	204	210	PF00498	0.621
LIG_FHA_2	380	386	PF00498	0.527
LIG_FHA_2	437	443	PF00498	0.515
LIG_FHA_2	471	477	PF00498	0.385
LIG_LIR_Apic_2	210	216	PF02991	0.572
LIG_LIR_Apic_2	501	505	PF02991	0.391
LIG_LIR_Gen_1	127	136	PF02991	0.493
LIG_LIR_Gen_1	479	487	PF02991	0.398
LIG_LIR_Gen_1	491	499	PF02991	0.356
LIG_LIR_Nem_3	127	132	PF02991	0.533
LIG_LIR_Nem_3	166	171	PF02991	0.343
LIG_LIR_Nem_3	475	480	PF02991	0.330
LIG_LIR_Nem_3	491	497	PF02991	0.437
LIG_Pex14_2	457	461	PF04695	0.322
LIG_Rb_pABgroove_1	517	525	PF01858	0.333
LIG_SH2_CRK	413	417	PF00017	0.466
LIG_SH2_NCK_1	186	190	PF00017	0.537
LIG_SH2_NCK_1	481	485	PF00017	0.315
LIG_SH2_SRC	481	484	PF00017	0.383
LIG_SH2_STAP1	186	190	PF00017	0.330
LIG_SH2_STAP1	255	259	PF00017	0.567
LIG_SH2_STAT3	37	40	PF00017	0.505
LIG_SH2_STAT3	411	414	PF00017	0.468
LIG_SH2_STAT5	170	173	PF00017	0.342
LIG_SH2_STAT5	255	258	PF00017	0.567
LIG_SH2_STAT5	276	279	PF00017	0.401
LIG_SH2_STAT5	510	513	PF00017	0.544
LIG_SH3_3	265	271	PF00018	0.494
LIG_SH3_3	528	534	PF00018	0.602
LIG_SUMO_SIM_anti_2	221	228	PF11976	0.419
LIG_SUMO_SIM_par_1	221	228	PF11976	0.431
LIG_SUMO_SIM_par_1	470	476	PF11976	0.407
LIG_UBA3_1	518	526	PF00899	0.337
MOD_CDC14_SPxK_1	215	218	PF00782	0.285
MOD_CDK_SPxK_1	212	218	PF00069	0.417
MOD_CDK_SPxxK_3	482	489	PF00069	0.257
MOD_CK1_1	107	113	PF00069	0.614
MOD_CK1_1	116	122	PF00069	0.537
MOD_CK1_1	127	133	PF00069	0.225
MOD_CK1_1	145	151	PF00069	0.547
MOD_CK1_1	173	179	PF00069	0.531
MOD_CK1_1	294	300	PF00069	0.648
MOD_CK2_1	366	372	PF00069	0.548
MOD_CK2_1	470	476	PF00069	0.459
MOD_CK2_1	482	488	PF00069	0.358
MOD_GlcNHglycan	115	118	PF01048	0.546
MOD_GlcNHglycan	120	123	PF01048	0.600
MOD_GlcNHglycan	163	166	PF01048	0.425
MOD_GlcNHglycan	176	179	PF01048	0.439
MOD_GlcNHglycan	277	280	PF01048	0.457
MOD_GlcNHglycan	309	312	PF01048	0.390
MOD_GlcNHglycan	507	510	PF01048	0.525
MOD_GlcNHglycan	511	514	PF01048	0.525
MOD_GSK3_1	138	145	PF00069	0.371
MOD_GSK3_1	170	177	PF00069	0.454
MOD_GSK3_1	199	206	PF00069	0.529
MOD_GSK3_1	2	9	PF00069	0.526
MOD_GSK3_1	249	256	PF00069	0.381
MOD_GSK3_1	290	297	PF00069	0.642
MOD_GSK3_1	298	305	PF00069	0.575
MOD_GSK3_1	366	373	PF00069	0.335
MOD_GSK3_1	505	512	PF00069	0.458
MOD_GSK3_1	56	63	PF00069	0.483
MOD_N-GLC_1	138	143	PF02516	0.581
MOD_N-GLC_1	161	166	PF02516	0.340
MOD_N-GLC_2	192	194	PF02516	0.418
MOD_NEK2_1	115	120	PF00069	0.440
MOD_NEK2_1	199	204	PF00069	0.383
MOD_NEK2_1	225	230	PF00069	0.426
MOD_NEK2_1	275	280	PF00069	0.487
MOD_NEK2_1	397	402	PF00069	0.404
MOD_NEK2_1	498	503	PF00069	0.446
MOD_NEK2_1	6	11	PF00069	0.550
MOD_NEK2_1	60	65	PF00069	0.536
MOD_NEK2_2	203	208	PF00069	0.481
MOD_NEK2_2	527	532	PF00069	0.650
MOD_PIKK_1	105	111	PF00454	0.612
MOD_PIKK_1	60	66	PF00454	0.573
MOD_PIKK_1	89	95	PF00454	0.409
MOD_PK_1	333	339	PF00069	0.470
MOD_PKA_2	125	131	PF00069	0.370
MOD_PKA_2	174	180	PF00069	0.475
MOD_PKA_2	290	296	PF00069	0.758
MOD_PKA_2	298	304	PF00069	0.592
MOD_PKA_2	397	403	PF00069	0.485
MOD_PKA_2	420	426	PF00069	0.459
MOD_Plk_1	138	144	PF00069	0.590
MOD_Plk_1	2	8	PF00069	0.429
MOD_Plk_4	145	151	PF00069	0.325
MOD_Plk_4	299	305	PF00069	0.643
MOD_Plk_4	428	434	PF00069	0.424
MOD_Plk_4	517	523	PF00069	0.389
MOD_Plk_4	533	539	PF00069	0.550
MOD_Plk_4	67	73	PF00069	0.537
MOD_ProDKin_1	12	18	PF00069	0.437
MOD_ProDKin_1	212	218	PF00069	0.405
MOD_ProDKin_1	366	372	PF00069	0.405
MOD_ProDKin_1	482	488	PF00069	0.339
MOD_SUMO_for_1	269	272	PF00179	0.428
MOD_SUMO_rev_2	419	429	PF00179	0.444
TRG_DiLeu_BaEn_1	372	377	PF01217	0.481
TRG_DiLeu_BaLyEn_6	514	519	PF01217	0.349
TRG_DiLeu_BaLyEn_6	546	551	PF01217	0.521
TRG_ENDOCYTIC_2	413	416	PF00928	0.413
TRG_ENDOCYTIC_2	481	484	PF00928	0.318
TRG_ENDOCYTIC_2	85	88	PF00928	0.516
TRG_ER_diArg_1	155	158	PF00400	0.430
TRG_ER_diArg_1	347	350	PF00400	0.573
TRG_ER_diArg_1	357	359	PF00400	0.461
TRG_ER_diArg_1	547	549	PF00400	0.377
TRG_NES_CRM1_1	392	407	PF08389	0.401
TRG_Pf-PMV_PEXEL_1	548	553	PF00026	0.473

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HW03	Leptomonas seymouri	62%	99%
A0A0S4JII0	Bodo saltans	27%	97%
A0A1X0P3R1	Trypanosomatidae	43%	100%
A0A3Q8IJI1	Leishmania donovani	84%	100%
A0A422NKK1	Trypanosoma rangeli	44%	100%
A4IDA0	Leishmania infantum	84%	100%
D0A8E0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	86%
E9AT90	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
Q4Q1A7	Leishmania major	85%	100%
V5AU55	Trypanosoma cruzi	41%	100%