Putative pre-mRNA branch site protein p14

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

pre-mRNA branch site protein p14, putative

Species:

Leishmania braziliensis

UniProt:

A4HPH0_LEIBR

TriTrypDb:

LbrM.35.3260 , LBRM2903_350041300

Length:

160

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005681	spliceosomal complex	3	1
GO:0005684	U2-type spliceosomal complex	4	1
GO:0005686	U2 snRNP	5	1
GO:0030532	small nuclear ribonucleoprotein complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0071011	precatalytic spliceosome	4	1
GO:0071013	catalytic step 2 spliceosome	3	1
GO:0097525	spliceosomal snRNP complex	4	1
GO:0120114	Sm-like protein family complex	2	1
GO:0140513	nuclear protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1990904	ribonucleoprotein complex	2	1

Expansion

Sequence features

A4HPH0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HPH0

Function

Biological processes
Term	Name	Level	Count
GO:0000375	RNA splicing, via transesterification reactions	8	1
GO:0000377	RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	9	1
GO:0000398	mRNA splicing, via spliceosome	8	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006397	mRNA processing	7	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0008380	RNA splicing	7	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0016071	mRNA metabolic process	6	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0005488	binding	1	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	133	135	PF00675	0.534
CLV_PCSK_FUR_1	133	137	PF00082	0.498
CLV_PCSK_KEX2_1	133	135	PF00082	0.481
CLV_PCSK_KEX2_1	137	139	PF00082	0.478
CLV_PCSK_KEX2_1	145	147	PF00082	0.467
CLV_PCSK_PC1ET2_1	135	137	PF00082	0.483
CLV_PCSK_PC1ET2_1	145	147	PF00082	0.467
CLV_PCSK_PC7_1	133	139	PF00082	0.481
CLV_PCSK_SKI1_1	128	132	PF00082	0.490
CLV_PCSK_SKI1_1	146	150	PF00082	0.458
CLV_PCSK_SKI1_1	47	51	PF00082	0.381
DEG_Nend_Nbox_1	1	3	PF02207	0.389
DOC_MAPK_gen_1	113	121	PF00069	0.437
DOC_MAPK_MEF2A_6	113	121	PF00069	0.422
DOC_PP4_FxxP_1	15	18	PF00568	0.370
DOC_WW_Pin1_4	19	24	PF00397	0.377
DOC_WW_Pin1_4	29	34	PF00397	0.374
LIG_14-3-3_CanoR_1	11	16	PF00244	0.515
LIG_14-3-3_CanoR_1	118	122	PF00244	0.393
LIG_14-3-3_CanoR_1	78	84	PF00244	0.458
LIG_APCC_ABBA_1	119	124	PF00400	0.419
LIG_APCC_ABBAyCdc20_2	118	124	PF00400	0.421
LIG_CtBP_PxDLS_1	18	22	PF00389	0.374
LIG_DCNL_PONY_1	1	4	PF03556	0.385
LIG_FHA_1	12	18	PF00498	0.428
LIG_LIR_Apic_2	13	18	PF02991	0.371
LIG_LIR_Gen_1	62	71	PF02991	0.500
LIG_LIR_Nem_3	62	68	PF02991	0.437
LIG_MAD2	47	55	PF02301	0.301
LIG_SH2_CRK	71	75	PF00017	0.448
LIG_SH2_SRC	63	66	PF00017	0.528
LIG_SH2_STAT5	63	66	PF00017	0.528
LIG_SUMO_SIM_anti_2	45	52	PF11976	0.399
LIG_SUMO_SIM_par_1	16	22	PF11976	0.634
LIG_SUMO_SIM_par_1	45	52	PF11976	0.399
LIG_SxIP_EBH_1	47	60	PF03271	0.301
MOD_CK1_1	22	28	PF00069	0.406
MOD_CK1_1	29	35	PF00069	0.460
MOD_CK1_1	82	88	PF00069	0.519
MOD_CK2_1	117	123	PF00069	0.547
MOD_GlcNHglycan	8	11	PF01048	0.382
MOD_GSK3_1	17	24	PF00069	0.522
MOD_GSK3_1	49	56	PF00069	0.301
MOD_GSK3_1	6	13	PF00069	0.625
MOD_GSK3_1	78	85	PF00069	0.479
MOD_NEK2_1	117	122	PF00069	0.394
MOD_NEK2_1	49	54	PF00069	0.301
MOD_NEK2_2	63	68	PF00069	0.528
MOD_PKA_2	10	16	PF00069	0.389
MOD_PKA_2	117	123	PF00069	0.395
MOD_Plk_4	117	123	PF00069	0.395
MOD_Plk_4	22	28	PF00069	0.634
MOD_Plk_4	63	69	PF00069	0.528
MOD_Plk_4	79	85	PF00069	0.424
MOD_ProDKin_1	19	25	PF00069	0.379
MOD_ProDKin_1	29	35	PF00069	0.374
MOD_SUMO_rev_2	123	131	PF00179	0.557
TRG_DiLeu_BaEn_1	46	51	PF01217	0.387
TRG_ENDOCYTIC_2	65	68	PF00928	0.448
TRG_ENDOCYTIC_2	71	74	PF00928	0.448
TRG_ER_diArg_1	133	136	PF00400	0.481
TRG_NLS_MonoCore_2	133	138	PF00514	0.481
TRG_NLS_MonoExtC_3	133	138	PF00514	0.481
TRG_NLS_MonoExtN_4	133	138	PF00514	0.481

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P798	Leptomonas seymouri	89%	100%
A0A1X0NKC5	Trypanosomatidae	66%	100%
A0A3Q8IHF1	Leishmania donovani	88%	88%
A0A3R7KER9	Trypanosoma rangeli	69%	100%
A4ID92	Leishmania infantum	88%	88%
D0A3I0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	62%	100%
E9AT82	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
Q4Q1B5	Leishmania major	86%	100%
Q8ITY4	Caenorhabditis elegans	34%	100%
V5BFU0	Trypanosoma cruzi	69%	100%