Separase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Separase

Gene product:

hypothetical protein, conserved

Species:

Leishmania braziliensis

UniProt:

A4HPD0_LEIBR

TriTrypDb:

LbrM.35.2850 , LBRM2903_350036100

Length:

551

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0032991	protein-containing complex	1	1
GO:0070390	transcription export complex 2	3	1
GO:0140513	nuclear protein-containing complex	2	1

Expansion

Sequence features

A4HPD0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HPD0

Function

Biological processes
Term	Name	Level	Count
GO:0006405	RNA export from nucleus	5	12
GO:0006406	mRNA export from nucleus	6	12
GO:0006810	transport	3	12
GO:0006913	nucleocytoplasmic transport	5	12
GO:0009987	cellular process	1	12
GO:0015931	nucleobase-containing compound transport	5	12
GO:0016973	poly(A)+ mRNA export from nucleus	7	12
GO:0046907	intracellular transport	3	12
GO:0050657	nucleic acid transport	6	12
GO:0050658	RNA transport	4	12
GO:0051028	mRNA transport	5	12
GO:0051168	nuclear export	6	12
GO:0051169	nuclear transport	4	12
GO:0051179	localization	1	12
GO:0051234	establishment of localization	2	12
GO:0051236	establishment of RNA localization	3	12
GO:0051641	cellular localization	2	12
GO:0051649	establishment of localization in cell	3	12
GO:0071702	organic substance transport	4	12
GO:0071705	nitrogen compound transport	4	12
GO:0000972	transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery	6	1
GO:0000973	post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery	7	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006354	DNA-templated transcription elongation	6	1
GO:0006368	transcription elongation by RNA polymerase II	7	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0006996	organelle organization	4	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009059	macromolecule biosynthetic process	4	1
GO:0016043	cellular component organization	3	1
GO:0016070	RNA metabolic process	5	1
GO:0018130	heterocycle biosynthetic process	4	1
GO:0019438	aromatic compound biosynthetic process	4	1
GO:0032774	RNA biosynthetic process	5	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034654	nucleobase-containing compound biosynthetic process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0046483	heterocycle metabolic process	3	1
GO:0051276	chromosome organization	5	1
GO:0071704	organic substance metabolic process	2	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	1
GO:0003677	DNA binding	4	1
GO:0003690	double-stranded DNA binding	5	1
GO:0003723	RNA binding	4	1
GO:0005488	binding	1	1
GO:0097159	organic cyclic compound binding	2	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	120	122	PF00675	0.508
CLV_NRD_NRD_1	235	237	PF00675	0.343
CLV_NRD_NRD_1	256	258	PF00675	0.355
CLV_NRD_NRD_1	328	330	PF00675	0.481
CLV_NRD_NRD_1	375	377	PF00675	0.511
CLV_NRD_NRD_1	421	423	PF00675	0.396
CLV_PCSK_KEX2_1	255	257	PF00082	0.363
CLV_PCSK_KEX2_1	375	377	PF00082	0.511
CLV_PCSK_KEX2_1	421	423	PF00082	0.394
CLV_PCSK_KEX2_1	486	488	PF00082	0.580
CLV_PCSK_PC1ET2_1	486	488	PF00082	0.584
CLV_PCSK_SKI1_1	122	126	PF00082	0.465
CLV_PCSK_SKI1_1	166	170	PF00082	0.362
CLV_PCSK_SKI1_1	236	240	PF00082	0.367
CLV_PCSK_SKI1_1	257	261	PF00082	0.326
CLV_PCSK_SKI1_1	301	305	PF00082	0.409
CLV_PCSK_SKI1_1	363	367	PF00082	0.393
CLV_PCSK_SKI1_1	511	515	PF00082	0.470
DEG_APCC_DBOX_1	235	243	PF00400	0.348
DEG_APCC_DBOX_1	256	264	PF00400	0.412
DEG_Nend_UBRbox_2	1	3	PF02207	0.551
DEG_SCF_FBW7_2	85	90	PF00400	0.490
DOC_CYCLIN_RxL_1	163	173	PF00134	0.380
DOC_CYCLIN_yCln2_LP_2	344	347	PF00134	0.349
DOC_MAPK_DCC_7	383	393	PF00069	0.273
DOC_MAPK_gen_1	233	242	PF00069	0.364
DOC_MAPK_gen_1	255	262	PF00069	0.363
DOC_MAPK_gen_1	34	43	PF00069	0.430
DOC_MAPK_gen_1	356	364	PF00069	0.343
DOC_MAPK_gen_1	421	428	PF00069	0.329
DOC_MAPK_MEF2A_6	101	109	PF00069	0.334
DOC_MAPK_MEF2A_6	233	242	PF00069	0.403
DOC_MAPK_MEF2A_6	34	43	PF00069	0.497
DOC_MAPK_MEF2A_6	421	428	PF00069	0.469
DOC_PP1_RVXF_1	359	365	PF00149	0.329
DOC_PP2B_LxvP_1	344	347	PF13499	0.374
DOC_USP7_MATH_1	171	175	PF00917	0.390
DOC_USP7_MATH_1	241	245	PF00917	0.454
DOC_USP7_MATH_1	251	255	PF00917	0.385
DOC_USP7_MATH_1	293	297	PF00917	0.572
DOC_USP7_MATH_1	299	303	PF00917	0.576
DOC_WW_Pin1_4	191	196	PF00397	0.545
DOC_WW_Pin1_4	492	497	PF00397	0.641
DOC_WW_Pin1_4	83	88	PF00397	0.578
LIG_14-3-3_CanoR_1	146	152	PF00244	0.628
LIG_14-3-3_CanoR_1	158	168	PF00244	0.462
LIG_14-3-3_CanoR_1	257	263	PF00244	0.486
LIG_14-3-3_CanoR_1	273	282	PF00244	0.280
LIG_14-3-3_CanoR_1	308	317	PF00244	0.501
LIG_14-3-3_CanoR_1	339	345	PF00244	0.407
LIG_14-3-3_CanoR_1	363	369	PF00244	0.333
LIG_14-3-3_CanoR_1	526	536	PF00244	0.353
LIG_14-3-3_CanoR_1	56	62	PF00244	0.475
LIG_14-3-3_CanoR_1	97	105	PF00244	0.502
LIG_BRCT_BRCA1_1	132	136	PF00533	0.534
LIG_EVH1_2	346	350	PF00568	0.537
LIG_FHA_1	108	114	PF00498	0.342
LIG_FHA_1	126	132	PF00498	0.532
LIG_FHA_1	160	166	PF00498	0.480
LIG_FHA_1	200	206	PF00498	0.315
LIG_FHA_1	395	401	PF00498	0.524
LIG_FHA_1	533	539	PF00498	0.414
LIG_FHA_1	84	90	PF00498	0.593
LIG_FHA_2	150	156	PF00498	0.567
LIG_FHA_2	469	475	PF00498	0.549
LIG_GBD_Chelix_1	262	270	PF00786	0.388
LIG_HCF-1_HBM_1	2	5	PF13415	0.470
LIG_Integrin_isoDGR_2	373	375	PF01839	0.481
LIG_Integrin_RGD_1	402	404	PF01839	0.324
LIG_LIR_Gen_1	197	208	PF02991	0.322
LIG_LIR_Gen_1	465	474	PF02991	0.305
LIG_LIR_Gen_1	9	20	PF02991	0.232
LIG_LIR_LC3C_4	102	105	PF02991	0.388
LIG_LIR_LC3C_4	535	538	PF02991	0.345
LIG_LIR_Nem_3	197	203	PF02991	0.334
LIG_LIR_Nem_3	2	8	PF02991	0.433
LIG_LIR_Nem_3	234	238	PF02991	0.332
LIG_LIR_Nem_3	316	320	PF02991	0.394
LIG_LIR_Nem_3	465	470	PF02991	0.286
LIG_LIR_Nem_3	9	15	PF02991	0.333
LIG_NRBOX	164	170	PF00104	0.489
LIG_PCNA_PIPBox_1	430	439	PF02747	0.356
LIG_PCNA_yPIPBox_3	430	442	PF02747	0.360
LIG_PDZ_Class_3	546	551	PF00595	0.522
LIG_SH2_CRK	342	346	PF00017	0.365
LIG_SH2_CRK	467	471	PF00017	0.427
LIG_SH2_STAP1	327	331	PF00017	0.347
LIG_SH2_STAP1	415	419	PF00017	0.245
LIG_SH2_STAT3	69	72	PF00017	0.443
LIG_SH2_STAT5	207	210	PF00017	0.458
LIG_SH2_STAT5	342	345	PF00017	0.398
LIG_SH2_STAT5	425	428	PF00017	0.336
LIG_SH2_STAT5	436	439	PF00017	0.259
LIG_SH2_STAT5	467	470	PF00017	0.446
LIG_SH2_STAT5	5	8	PF00017	0.463
LIG_SH2_STAT5	524	527	PF00017	0.339
LIG_SH2_STAT5	69	72	PF00017	0.535
LIG_SH3_3	539	545	PF00018	0.510
LIG_SUMO_SIM_anti_2	201	207	PF11976	0.330
LIG_SUMO_SIM_par_1	238	244	PF11976	0.457
LIG_SUMO_SIM_par_1	534	539	PF11976	0.347
LIG_TRAF2_1	152	155	PF00917	0.354
LIG_TRAF2_1	174	177	PF00917	0.396
LIG_TRAF2_1	295	298	PF00917	0.470
LIG_TRAF2_1	471	474	PF00917	0.415
LIG_TRFH_1	342	346	PF08558	0.337
LIG_TYR_ITIM	340	345	PF00017	0.405
LIG_TYR_ITIM	434	439	PF00017	0.383
LIG_TYR_ITSM	463	470	PF00017	0.370
LIG_WRC_WIRS_1	172	177	PF05994	0.408
LIG_WW_3	186	190	PF00397	0.589
MOD_CK1_1	130	136	PF00069	0.674
MOD_CK1_1	159	165	PF00069	0.426
MOD_CK1_1	456	462	PF00069	0.507
MOD_CK1_1	47	53	PF00069	0.544
MOD_CK1_1	83	89	PF00069	0.575
MOD_CK2_1	149	155	PF00069	0.420
MOD_CK2_1	171	177	PF00069	0.403
MOD_CK2_1	292	298	PF00069	0.563
MOD_CK2_1	468	474	PF00069	0.503
MOD_CK2_1	96	102	PF00069	0.407
MOD_Cter_Amidation	373	376	PF01082	0.496
MOD_GlcNHglycan	219	222	PF01048	0.528
MOD_GlcNHglycan	408	411	PF01048	0.462
MOD_GlcNHglycan	46	49	PF01048	0.460
MOD_GlcNHglycan	476	479	PF01048	0.653
MOD_GlcNHglycan	480	483	PF01048	0.638
MOD_GSK3_1	107	114	PF00069	0.486
MOD_GSK3_1	125	132	PF00069	0.568
MOD_GSK3_1	138	145	PF00069	0.734
MOD_GSK3_1	156	163	PF00069	0.430
MOD_GSK3_1	309	316	PF00069	0.494
MOD_GSK3_1	411	418	PF00069	0.413
MOD_GSK3_1	465	472	PF00069	0.500
MOD_GSK3_1	474	481	PF00069	0.628
MOD_GSK3_1	532	539	PF00069	0.372
MOD_GSK3_1	54	61	PF00069	0.473
MOD_GSK3_1	73	80	PF00069	0.391
MOD_N-GLC_1	44	49	PF02516	0.555
MOD_NEK2_1	125	130	PF00069	0.605
MOD_NEK2_1	131	136	PF00069	0.665
MOD_NEK2_1	156	161	PF00069	0.501
MOD_NEK2_1	364	369	PF00069	0.355
MOD_NEK2_1	502	507	PF00069	0.632
MOD_NEK2_1	536	541	PF00069	0.501
MOD_NEK2_1	54	59	PF00069	0.499
MOD_NEK2_1	77	82	PF00069	0.599
MOD_NEK2_2	415	420	PF00069	0.250
MOD_PIKK_1	293	299	PF00454	0.464
MOD_PKA_1	485	491	PF00069	0.694
MOD_PKA_2	117	123	PF00069	0.458
MOD_PKA_2	456	462	PF00069	0.406
MOD_PKA_2	55	61	PF00069	0.488
MOD_PKA_2	77	83	PF00069	0.583
MOD_PKA_2	96	102	PF00069	0.319
MOD_Plk_1	156	162	PF00069	0.429
MOD_Plk_1	224	230	PF00069	0.401
MOD_Plk_1	453	459	PF00069	0.353
MOD_Plk_4	160	166	PF00069	0.433
MOD_Plk_4	199	205	PF00069	0.319
MOD_Plk_4	258	264	PF00069	0.377
MOD_Plk_4	340	346	PF00069	0.474
MOD_Plk_4	465	471	PF00069	0.446
MOD_Plk_4	502	508	PF00069	0.551
MOD_ProDKin_1	191	197	PF00069	0.533
MOD_ProDKin_1	492	498	PF00069	0.638
MOD_ProDKin_1	83	89	PF00069	0.571
MOD_SUMO_for_1	33	36	PF00179	0.474
TRG_DiLeu_BaEn_1	532	537	PF01217	0.444
TRG_DiLeu_BaLyEn_6	387	392	PF01217	0.264
TRG_DiLeu_BaLyEn_6	542	547	PF01217	0.498
TRG_ENDOCYTIC_2	342	345	PF00928	0.364
TRG_ENDOCYTIC_2	425	428	PF00928	0.375
TRG_ENDOCYTIC_2	436	439	PF00928	0.307
TRG_ENDOCYTIC_2	467	470	PF00928	0.367
TRG_ENDOCYTIC_2	5	8	PF00928	0.463
TRG_ER_diArg_1	255	257	PF00400	0.363
TRG_ER_diArg_1	323	326	PF00400	0.409
TRG_ER_diArg_1	361	364	PF00400	0.335
TRG_ER_diArg_1	375	377	PF00400	0.511
TRG_NLS_MonoExtC_3	484	489	PF00514	0.578
TRG_Pf-PMV_PEXEL_1	222	226	PF00026	0.425
TRG_Pf-PMV_PEXEL_1	68	72	PF00026	0.509

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HTB0	Leptomonas seymouri	70%	100%
A0A0S4J9T0	Bodo saltans	44%	99%
A0A1X0NKD6	Trypanosomatidae	45%	100%
A0A3R7LU45	Trypanosoma rangeli	45%	100%
A0A3S7XAY0	Leishmania donovani	90%	100%
A4ICH0	Leishmania infantum	90%	100%
D0A3J0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	99%
E9AT41	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
Q4Q1F7	Leishmania major	90%	100%
V5BKA5	Trypanosoma cruzi	42%	100%