Folylpolyglutamate synthetase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

folylpolyglutamate synthetase

Species:

Leishmania braziliensis

UniProt:

A4HPC7_LEIBR

TriTrypDb:

LbrM.35.2820 , LBRM2903_350035800

Length:

530

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005739	mitochondrion	5	1
GO:0005829	cytosol	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HPC7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HPC7

Function

Biological processes
Term	Name	Level	Count
GO:0006575	cellular modified amino acid metabolic process	3	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006760	folic acid-containing compound metabolic process	4	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009396	folic acid-containing compound biosynthetic process	5	1
GO:0009987	cellular process	1	1
GO:0018130	heterocycle biosynthetic process	4	1
GO:0019438	aromatic compound biosynthetic process	4	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0042398	cellular modified amino acid biosynthetic process	4	1
GO:0042558	pteridine-containing compound metabolic process	4	1
GO:0042559	pteridine-containing compound biosynthetic process	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0046483	heterocycle metabolic process	3	1
GO:0046900	tetrahydrofolylpolyglutamate metabolic process	5	1
GO:0046901	tetrahydrofolylpolyglutamate biosynthetic process	6	1
GO:0071704	organic substance metabolic process	2	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901566	organonitrogen compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004326	tetrahydrofolylpolyglutamate synthase activity	5	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	12
GO:0016881	acid-amino acid ligase activity	4	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	141	145	PF00656	0.386
CLV_C14_Caspase3-7	55	59	PF00656	0.477
CLV_NRD_NRD_1	322	324	PF00675	0.349
CLV_NRD_NRD_1	395	397	PF00675	0.328
CLV_NRD_NRD_1	527	529	PF00675	0.554
CLV_NRD_NRD_1	69	71	PF00675	0.631
CLV_PCSK_KEX2_1	322	324	PF00082	0.349
CLV_PCSK_KEX2_1	395	397	PF00082	0.336
CLV_PCSK_KEX2_1	526	528	PF00082	0.551
CLV_PCSK_KEX2_1	69	71	PF00082	0.587
CLV_PCSK_KEX2_1	82	84	PF00082	0.511
CLV_PCSK_PC1ET2_1	82	84	PF00082	0.409
CLV_PCSK_SKI1_1	188	192	PF00082	0.323
CLV_PCSK_SKI1_1	273	277	PF00082	0.384
CLV_PCSK_SKI1_1	315	319	PF00082	0.285
CLV_PCSK_SKI1_1	338	342	PF00082	0.326
CLV_PCSK_SKI1_1	395	399	PF00082	0.375
CLV_PCSK_SKI1_1	407	411	PF00082	0.315
CLV_PCSK_SKI1_1	466	470	PF00082	0.313
CLV_PCSK_SKI1_1	54	58	PF00082	0.529
CLV_PCSK_SKI1_1	64	68	PF00082	0.426
DEG_SCF_TRCP1_1	356	362	PF00400	0.290
DOC_ANK_TNKS_1	139	146	PF00023	0.431
DOC_CDC14_PxL_1	88	96	PF14671	0.387
DOC_CKS1_1	283	288	PF01111	0.388
DOC_CYCLIN_RxL_1	390	403	PF00134	0.379
DOC_MAPK_DCC_7	323	333	PF00069	0.397
DOC_MAPK_gen_1	54	62	PF00069	0.553
DOC_MAPK_gen_1	82	90	PF00069	0.469
DOC_MAPK_MEF2A_6	188	197	PF00069	0.353
DOC_MAPK_MEF2A_6	281	290	PF00069	0.360
DOC_PP1_RVXF_1	85	91	PF00149	0.374
DOC_PP1_RVXF_1	94	100	PF00149	0.278
DOC_PP1_SILK_1	407	412	PF00149	0.230
DOC_PP2B_LxvP_1	486	489	PF13499	0.487
DOC_PP4_FxxP_1	129	132	PF00568	0.293
DOC_PP4_FxxP_1	262	265	PF00568	0.408
DOC_USP7_MATH_1	249	253	PF00917	0.172
DOC_USP7_MATH_1	296	300	PF00917	0.508
DOC_USP7_MATH_1	30	34	PF00917	0.535
DOC_USP7_MATH_1	347	351	PF00917	0.364
DOC_USP7_MATH_1	354	358	PF00917	0.414
DOC_USP7_MATH_1	415	419	PF00917	0.418
DOC_USP7_MATH_1	426	430	PF00917	0.516
DOC_USP7_MATH_1	489	493	PF00917	0.528
DOC_USP7_UBL2_3	407	411	PF12436	0.458
DOC_WW_Pin1_4	128	133	PF00397	0.295
DOC_WW_Pin1_4	17	22	PF00397	0.712
DOC_WW_Pin1_4	177	182	PF00397	0.560
DOC_WW_Pin1_4	282	287	PF00397	0.333
DOC_WW_Pin1_4	39	44	PF00397	0.733
DOC_WW_Pin1_4	390	395	PF00397	0.415
LIG_14-3-3_CanoR_1	432	440	PF00244	0.573
LIG_14-3-3_CanoR_1	453	461	PF00244	0.483
LIG_14-3-3_CanoR_1	475	484	PF00244	0.514
LIG_Clathr_ClatBox_1	287	291	PF01394	0.409
LIG_FHA_1	217	223	PF00498	0.291
LIG_FHA_1	270	276	PF00498	0.378
LIG_FHA_1	283	289	PF00498	0.419
LIG_FHA_1	360	366	PF00498	0.354
LIG_FHA_2	139	145	PF00498	0.395
LIG_FHA_2	241	247	PF00498	0.284
LIG_FHA_2	400	406	PF00498	0.414
LIG_FHA_2	439	445	PF00498	0.468
LIG_HP1_1	286	290	PF01393	0.389
LIG_LIR_Gen_1	231	240	PF02991	0.322
LIG_LIR_Gen_1	378	387	PF02991	0.410
LIG_LIR_Gen_1	418	428	PF02991	0.399
LIG_LIR_LC3C_4	485	488	PF02991	0.478
LIG_LIR_Nem_3	162	167	PF02991	0.418
LIG_LIR_Nem_3	299	303	PF02991	0.399
LIG_LIR_Nem_3	378	384	PF02991	0.398
LIG_LIR_Nem_3	418	423	PF02991	0.335
LIG_LIR_Nem_3	463	468	PF02991	0.277
LIG_LIR_Nem_3	508	512	PF02991	0.332
LIG_Pex14_1	461	465	PF04695	0.327
LIG_Pex14_2	187	191	PF04695	0.272
LIG_REV1ctd_RIR_1	184	192	PF16727	0.348
LIG_SH2_SRC	364	367	PF00017	0.343
LIG_SH2_STAP1	153	157	PF00017	0.421
LIG_SH2_STAT5	114	117	PF00017	0.271
LIG_SH2_STAT5	196	199	PF00017	0.354
LIG_SH2_STAT5	364	367	PF00017	0.299
LIG_SH2_STAT5	401	404	PF00017	0.393
LIG_SH2_STAT5	416	419	PF00017	0.369
LIG_SH2_STAT5	84	87	PF00017	0.410
LIG_SH3_3	280	286	PF00018	0.351
LIG_SH3_3	326	332	PF00018	0.400
LIG_SH3_3	425	431	PF00018	0.328
LIG_SH3_3	43	49	PF00018	0.564
LIG_SUMO_SIM_anti_2	204	209	PF11976	0.244
LIG_SUMO_SIM_anti_2	224	231	PF11976	0.288
LIG_SUMO_SIM_anti_2	285	291	PF11976	0.472
LIG_SUMO_SIM_anti_2	314	320	PF11976	0.297
LIG_SUMO_SIM_anti_2	481	488	PF11976	0.418
LIG_SUMO_SIM_par_1	285	291	PF11976	0.399
LIG_SUMO_SIM_par_1	396	403	PF11976	0.241
LIG_TRAF2_1	243	246	PF00917	0.249
LIG_UBA3_1	330	338	PF00899	0.491
LIG_WRC_WIRS_1	53	58	PF05994	0.281
MOD_CDC14_SPxK_1	393	396	PF00782	0.380
MOD_CDK_SPK_2	390	395	PF00069	0.415
MOD_CDK_SPxK_1	390	396	PF00069	0.407
MOD_CK1_1	179	185	PF00069	0.379
MOD_CK1_1	218	224	PF00069	0.363
MOD_CK1_1	227	233	PF00069	0.314
MOD_CK1_1	373	379	PF00069	0.461
MOD_CK2_1	240	246	PF00069	0.249
MOD_CK2_1	438	444	PF00069	0.536
MOD_CK2_1	475	481	PF00069	0.347
MOD_CK2_1	489	495	PF00069	0.369
MOD_Cter_Amidation	524	527	PF01082	0.535
MOD_Cter_Amidation	67	70	PF01082	0.509
MOD_GlcNHglycan	111	114	PF01048	0.357
MOD_GlcNHglycan	13	16	PF01048	0.705
MOD_GlcNHglycan	250	254	PF01048	0.407
MOD_GlcNHglycan	356	359	PF01048	0.350
MOD_GlcNHglycan	375	378	PF01048	0.424
MOD_GlcNHglycan	383	387	PF01048	0.443
MOD_GlcNHglycan	436	439	PF01048	0.654
MOD_GlcNHglycan	477	480	PF01048	0.487
MOD_GSK3_1	11	18	PF00069	0.710
MOD_GSK3_1	128	135	PF00069	0.470
MOD_GSK3_1	224	231	PF00069	0.335
MOD_GSK3_1	382	389	PF00069	0.396
MOD_GSK3_1	434	441	PF00069	0.531
MOD_N-GLC_1	359	364	PF02516	0.392
MOD_N-GLC_2	72	74	PF02516	0.603
MOD_NEK2_1	148	153	PF00069	0.342
MOD_NEK2_1	191	196	PF00069	0.285
MOD_NEK2_1	240	245	PF00069	0.393
MOD_NEK2_1	382	387	PF00069	0.409
MOD_NEK2_1	399	404	PF00069	0.260
MOD_NEK2_1	78	83	PF00069	0.352
MOD_NEK2_2	2	7	PF00069	0.700
MOD_NEK2_2	359	364	PF00069	0.367
MOD_NEK2_2	460	465	PF00069	0.233
MOD_NEK2_2	52	57	PF00069	0.498
MOD_PIKK_1	169	175	PF00454	0.481
MOD_PIKK_1	179	185	PF00454	0.424
MOD_PIKK_1	347	353	PF00454	0.330
MOD_PIKK_1	375	381	PF00454	0.448
MOD_PK_1	323	329	PF00069	0.304
MOD_PKA_1	64	70	PF00069	0.347
MOD_PKA_2	431	437	PF00069	0.555
MOD_Plk_1	138	144	PF00069	0.492
MOD_Plk_1	148	154	PF00069	0.407
MOD_Plk_1	224	230	PF00069	0.284
MOD_Plk_1	336	342	PF00069	0.395
MOD_Plk_1	359	365	PF00069	0.445
MOD_Plk_1	466	472	PF00069	0.394
MOD_Plk_4	191	197	PF00069	0.309
MOD_Plk_4	224	230	PF00069	0.284
MOD_Plk_4	336	342	PF00069	0.441
MOD_Plk_4	405	411	PF00069	0.384
MOD_Plk_4	415	421	PF00069	0.304
MOD_Plk_4	505	511	PF00069	0.319
MOD_ProDKin_1	128	134	PF00069	0.297
MOD_ProDKin_1	17	23	PF00069	0.710
MOD_ProDKin_1	177	183	PF00069	0.559
MOD_ProDKin_1	282	288	PF00069	0.333
MOD_ProDKin_1	39	45	PF00069	0.732
MOD_ProDKin_1	390	396	PF00069	0.407
MOD_SUMO_rev_2	403	409	PF00179	0.474
MOD_SUMO_rev_2	439	448	PF00179	0.610
TRG_DiLeu_BaEn_2	94	100	PF01217	0.396
TRG_DiLeu_BaLyEn_6	143	148	PF01217	0.400
TRG_ENDOCYTIC_2	164	167	PF00928	0.451
TRG_ENDOCYTIC_2	234	237	PF00928	0.393
TRG_ENDOCYTIC_2	465	468	PF00928	0.358
TRG_ENDOCYTIC_2	53	56	PF00928	0.347
TRG_ER_diArg_1	322	324	PF00400	0.349
TRG_ER_diArg_1	394	396	PF00400	0.361
TRG_ER_diArg_1	526	528	PF00400	0.475
TRG_ER_diArg_1	69	71	PF00400	0.532
TRG_ER_diArg_1	96	99	PF00400	0.368
TRG_Pf-PMV_PEXEL_1	146	150	PF00026	0.440

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6C4	Leptomonas seymouri	72%	81%
A0A0S4KJ93	Bodo saltans	47%	100%
A0A1X0NM42	Trypanosomatidae	54%	100%
A0A3Q8IJ59	Leishmania donovani	85%	98%
A0A3R7RIQ8	Trypanosoma rangeli	53%	100%
A4ICG7	Leishmania infantum	85%	98%
A6H751	Bos taurus	32%	91%
A6ZP80	Saccharomyces cerevisiae (strain YJM789)	30%	97%
B3LJR0	Saccharomyces cerevisiae (strain RM11-1a)	30%	97%
B5VSC3	Saccharomyces cerevisiae (strain AWRI1631)	30%	97%
C8ZGZ3	Saccharomyces cerevisiae (strain Lalvin EC1118 / Prise de mousse)	30%	97%
D0A3I6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	100%
E7KIA3	Saccharomyces cerevisiae (strain AWRI796)	30%	97%
E7KUJ4	Saccharomyces cerevisiae (strain Lalvin QA23)	30%	97%
E7NMM0	Saccharomyces cerevisiae (strain FostersO)	30%	97%
E7Q9C7	Saccharomyces cerevisiae (strain FostersB)	30%	97%
E7QKX4	Saccharomyces cerevisiae (strain Zymaflore VL3)	30%	97%
E9AT38	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	99%
F4J2K2	Arabidopsis thaliana	30%	85%
F4K2A1	Arabidopsis thaliana	29%	93%
O13492	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	29%	100%
O74742	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	100%
P48760	Mus musculus	31%	90%
Q05932	Homo sapiens	31%	90%
Q08645	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	30%	97%
Q09509	Caenorhabditis elegans	29%	100%
Q12676	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	100%
Q4Q1G0	Leishmania major	85%	100%
Q54CY5	Dictyostelium discoideum	29%	85%
Q8W035	Arabidopsis thaliana	31%	100%
Q924L9	Cricetulus griseus	29%	90%
Q9UTD0	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%
Q9Y893	Candida albicans	31%	100%
V5BKB0	Trypanosoma cruzi	51%	100%