Putative C-4 sterol methyl oxidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

C-4 sterol methyl oxidase, putative

Species:

Leishmania braziliensis

UniProt:

A4HPC0_LEIBR

TriTrypDb:

LbrM.35.2750 , LBRM2903_350034600

Length:

393

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	7
GO:0110165	cellular anatomical entity	1	7
GO:0005789	endoplasmic reticulum membrane	4	1
GO:0031090	organelle membrane	3	1

Expansion

Sequence features

A4HPC0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HPC0

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	8
GO:0008152	metabolic process	1	8
GO:0008610	lipid biosynthetic process	4	8
GO:0009058	biosynthetic process	2	8
GO:0044238	primary metabolic process	2	8
GO:0071704	organic substance metabolic process	2	8
GO:1901576	organic substance biosynthetic process	3	8
GO:0006694	steroid biosynthetic process	5	1
GO:0008202	steroid metabolic process	4	1
GO:0016125	sterol metabolic process	4	1
GO:0016126	sterol biosynthetic process	5	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901615	organic hydroxy compound metabolic process	3	1
GO:1901617	organic hydroxy compound biosynthetic process	4	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	8
GO:0005488	binding	1	8
GO:0005506	iron ion binding	6	8
GO:0016491	oxidoreductase activity	2	8
GO:0043167	ion binding	2	8
GO:0043169	cation binding	3	8
GO:0046872	metal ion binding	4	8
GO:0046914	transition metal ion binding	5	8
GO:0000254	C-4 methylsterol oxidase activity	5	1
GO:0004497	monooxygenase activity	3	1
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	3	1
GO:0016716	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, another compound as one donor, and incorporation of one atom of oxygen	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	115	117	PF00675	0.402
CLV_NRD_NRD_1	123	125	PF00675	0.297
CLV_PCSK_KEX2_1	115	117	PF00082	0.360
CLV_PCSK_SKI1_1	189	193	PF00082	0.586
CLV_PCSK_SKI1_1	197	201	PF00082	0.490
CLV_PCSK_SKI1_1	6	10	PF00082	0.464
DEG_MDM2_SWIB_1	315	323	PF02201	0.308
DEG_Nend_UBRbox_2	1	3	PF02207	0.641
DOC_CKS1_1	250	255	PF01111	0.308
DOC_CKS1_1	63	68	PF01111	0.285
DOC_CYCLIN_yCln2_LP_2	148	154	PF00134	0.354
DOC_CYCLIN_yCln2_LP_2	63	69	PF00134	0.350
DOC_MAPK_gen_1	189	198	PF00069	0.364
DOC_PP2B_LxvP_1	109	112	PF13499	0.546
DOC_PP2B_LxvP_1	148	151	PF13499	0.363
DOC_USP7_MATH_1	280	284	PF00917	0.302
DOC_USP7_UBL2_3	121	125	PF12436	0.557
DOC_USP7_UBL2_3	5	9	PF12436	0.667
DOC_WW_Pin1_4	175	180	PF00397	0.444
DOC_WW_Pin1_4	249	254	PF00397	0.302
DOC_WW_Pin1_4	62	67	PF00397	0.352
LIG_14-3-3_CanoR_1	17	25	PF00244	0.572
LIG_14-3-3_CanoR_1	371	379	PF00244	0.426
LIG_BRCT_BRCA1_1	268	272	PF00533	0.203
LIG_BRCT_BRCA1_1	346	350	PF00533	0.302
LIG_BRCT_BRCA1_1	86	90	PF00533	0.216
LIG_FHA_1	154	160	PF00498	0.423
LIG_FHA_1	239	245	PF00498	0.265
LIG_FHA_1	304	310	PF00498	0.308
LIG_FHA_1	337	343	PF00498	0.259
LIG_FHA_1	36	42	PF00498	0.411
LIG_FHA_1	384	390	PF00498	0.409
LIG_FHA_1	63	69	PF00498	0.281
LIG_FHA_2	176	182	PF00498	0.378
LIG_FHA_2	250	256	PF00498	0.308
LIG_FHA_2	274	280	PF00498	0.308
LIG_FHA_2	373	379	PF00498	0.452
LIG_FHA_2	382	388	PF00498	0.461
LIG_LIR_Apic_2	249	253	PF02991	0.302
LIG_LIR_Gen_1	218	227	PF02991	0.380
LIG_LIR_Gen_1	269	275	PF02991	0.287
LIG_LIR_Gen_1	297	307	PF02991	0.302
LIG_LIR_Nem_3	213	219	PF02991	0.346
LIG_LIR_Nem_3	264	270	PF02991	0.295
LIG_LIR_Nem_3	297	303	PF02991	0.302
LIG_LIR_Nem_3	70	76	PF02991	0.334
LIG_LIR_Nem_3	87	92	PF02991	0.218
LIG_MLH1_MIPbox_1	86	90	PF16413	0.216
LIG_OCRL_FandH_1	232	244	PF00620	0.302
LIG_Pex14_2	268	272	PF04695	0.302
LIG_Pex14_2	315	319	PF04695	0.302
LIG_Pex14_2	69	73	PF04695	0.354
LIG_Pex14_2	85	89	PF04695	0.354
LIG_PTB_Apo_2	83	90	PF02174	0.354
LIG_SH2_CRK	120	124	PF00017	0.549
LIG_SH2_CRK	155	159	PF00017	0.363
LIG_SH2_CRK	250	254	PF00017	0.259
LIG_SH2_CRK	50	54	PF00017	0.400
LIG_SH2_GRB2like	160	163	PF00017	0.296
LIG_SH2_SRC	361	364	PF00017	0.277
LIG_SH2_STAP1	155	159	PF00017	0.363
LIG_SH2_STAT5	147	150	PF00017	0.346
LIG_SH2_STAT5	155	158	PF00017	0.202
LIG_SH2_STAT5	160	163	PF00017	0.331
LIG_SH2_STAT5	217	220	PF00017	0.259
LIG_SH2_STAT5	240	243	PF00017	0.259
LIG_SH2_STAT5	267	270	PF00017	0.307
LIG_SH2_STAT5	275	278	PF00017	0.311
LIG_SH2_STAT5	30	33	PF00017	0.354
LIG_SH2_STAT5	313	316	PF00017	0.271
LIG_SH2_STAT5	361	364	PF00017	0.333
LIG_SH2_STAT5	59	62	PF00017	0.416
LIG_SH3_3	42	48	PF00018	0.306
LIG_SH3_3	5	11	PF00018	0.632
LIG_SUMO_SIM_par_1	150	156	PF11976	0.247
LIG_SUMO_SIM_par_1	197	203	PF11976	0.343
LIG_TRAF2_1	276	279	PF00917	0.308
LIG_TYR_ITIM	153	158	PF00017	0.427
LIG_WRC_WIRS_1	216	221	PF05994	0.203
LIG_WRC_WIRS_1	267	272	PF05994	0.243
MOD_CK2_1	249	255	PF00069	0.302
MOD_CK2_1	273	279	PF00069	0.385
MOD_Cter_Amidation	113	116	PF01082	0.352
MOD_GlcNHglycan	130	133	PF01048	0.450
MOD_GlcNHglycan	186	189	PF01048	0.611
MOD_GlcNHglycan	282	285	PF01048	0.508
MOD_GlcNHglycan	287	290	PF01048	0.537
MOD_GlcNHglycan	310	313	PF01048	0.459
MOD_GlcNHglycan	346	349	PF01048	0.502
MOD_GSK3_1	135	142	PF00069	0.427
MOD_GSK3_1	218	225	PF00069	0.318
MOD_GSK3_1	238	245	PF00069	0.335
MOD_GSK3_1	280	287	PF00069	0.324
MOD_N-GLC_2	162	164	PF02516	0.596
MOD_N-GLC_2	341	343	PF02516	0.508
MOD_NEK2_1	1	6	PF00069	0.663
MOD_NEK2_1	139	144	PF00069	0.326
MOD_NEK2_1	158	163	PF00069	0.543
MOD_NEK2_1	285	290	PF00069	0.354
MOD_NEK2_1	294	299	PF00069	0.265
MOD_NEK2_1	315	320	PF00069	0.265
MOD_NEK2_1	336	341	PF00069	0.302
MOD_NEK2_1	55	60	PF00069	0.376
MOD_NEK2_1	84	89	PF00069	0.331
MOD_NEK2_1	91	96	PF00069	0.592
MOD_NEK2_2	12	17	PF00069	0.488
MOD_NEK2_2	215	220	PF00069	0.222
MOD_OFUCOSY	157	164	PF10250	0.336
MOD_PIKK_1	372	378	PF00454	0.389
MOD_PKA_2	370	376	PF00069	0.455
MOD_Plk_4	135	141	PF00069	0.357
MOD_Plk_4	218	224	PF00069	0.303
MOD_Plk_4	294	300	PF00069	0.319
MOD_Plk_4	315	321	PF00069	0.302
MOD_Plk_4	55	61	PF00069	0.467
MOD_Plk_4	84	90	PF00069	0.211
MOD_ProDKin_1	175	181	PF00069	0.440
MOD_ProDKin_1	249	255	PF00069	0.302
MOD_ProDKin_1	62	68	PF00069	0.346
TRG_ENDOCYTIC_2	147	150	PF00928	0.354
TRG_ENDOCYTIC_2	155	158	PF00928	0.234
TRG_ENDOCYTIC_2	230	233	PF00928	0.259
TRG_ENDOCYTIC_2	267	270	PF00928	0.293
TRG_ENDOCYTIC_2	50	53	PF00928	0.320
TRG_ER_diArg_1	115	117	PF00400	0.564
TRG_ER_diArg_1	330	333	PF00400	0.302

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEE3	Leptomonas seymouri	60%	98%
A0A0S4JXU3	Bodo saltans	36%	100%
A0A3Q8IKR9	Leishmania donovani	79%	98%
A4ICG0	Leishmania infantum	79%	98%
E9AT31	Leishmania mexicana (strain MHOM/GT/2001/U1103)	75%	98%
Q4Q1G7	Leishmania major	76%	100%