Putative ER--golgi transport protein erv25

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

transmembrane emp24 domain-containing protein

Species:

Leishmania braziliensis

UniProt:

A4HP84_LEIBR

TriTrypDb:

LbrM.35.2390 , LBRM2903_350030500

Length:

268

Annotations

LeishMANIAdb annotations

Conserved eukaryotic protein. Probable receptor for ER-Golgi trafficking of GPI-anchored proteins. Related to metazoan Tmed10.. Localization: ER (by homology) / Golgi (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 25

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 10, no: 0
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	10
GO:0110165	cellular anatomical entity	1	10
GO:0005783	endoplasmic reticulum	5	1
GO:0005793	endoplasmic reticulum-Golgi intermediate compartment	5	1
GO:0005794	Golgi apparatus	5	1
GO:0030134	COPII-coated ER to Golgi transport vesicle	8	1
GO:0030135	coated vesicle	7	1
GO:0031410	cytoplasmic vesicle	6	1
GO:0031982	vesicle	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0097708	intracellular vesicle	5	1

Expansion

Sequence features

A4HP84

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HP84

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	1
GO:0006886	intracellular protein transport	4	1
GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport	4	1
GO:0006996	organelle organization	4	1
GO:0007030	Golgi organization	5	1
GO:0008104	protein localization	4	1
GO:0009987	cellular process	1	1
GO:0015031	protein transport	4	1
GO:0016043	cellular component organization	3	1
GO:0016192	vesicle-mediated transport	4	1
GO:0033036	macromolecule localization	2	1
GO:0045184	establishment of protein localization	3	1
GO:0046907	intracellular transport	3	1
GO:0048193	Golgi vesicle transport	5	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0051641	cellular localization	2	1
GO:0051649	establishment of localization in cell	3	1
GO:0070727	cellular macromolecule localization	3	1
GO:0071702	organic substance transport	4	1
GO:0071705	nitrogen compound transport	4	1
GO:0071840	cellular component organization or biogenesis	2	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	87	91	PF00656	0.418
CLV_NRD_NRD_1	155	157	PF00675	0.586
CLV_NRD_NRD_1	166	168	PF00675	0.588
CLV_NRD_NRD_1	262	264	PF00675	0.484
CLV_PCSK_KEX2_1	165	167	PF00082	0.521
CLV_PCSK_PC1ET2_1	165	167	PF00082	0.521
CLV_PCSK_SKI1_1	185	189	PF00082	0.521
CLV_PCSK_SKI1_1	20	24	PF00082	0.688
CLV_PCSK_SKI1_1	256	260	PF00082	0.331
DEG_APCC_DBOX_1	190	198	PF00400	0.387
DEG_MDM2_SWIB_1	233	241	PF02201	0.387
DOC_ANK_TNKS_1	165	172	PF00023	0.274
DOC_MAPK_gen_1	165	174	PF00069	0.332
DOC_MAPK_MEF2A_6	136	145	PF00069	0.408
DOC_MAPK_MEF2A_6	20	29	PF00069	0.506
DOC_PP1_RVXF_1	47	54	PF00149	0.387
DOC_USP7_MATH_1	34	38	PF00917	0.574
DOC_USP7_UBL2_3	136	140	PF12436	0.230
DOC_USP7_UBL2_3	150	154	PF12436	0.191
DOC_USP7_UBL2_3	157	161	PF12436	0.211
DOC_WW_Pin1_4	74	79	PF00397	0.410
LIG_14-3-3_CanoR_1	85	89	PF00244	0.278
LIG_Actin_WH2_2	243	261	PF00022	0.395
LIG_BRCT_BRCA1_1	127	131	PF00533	0.411
LIG_CORNRBOX	243	251	PF00104	0.505
LIG_FHA_1	214	220	PF00498	0.258
LIG_FHA_1	90	96	PF00498	0.366
LIG_FHA_2	174	180	PF00498	0.307
LIG_FXI_DFP_1	15	19	PF00024	0.561
LIG_LIR_Gen_1	176	184	PF02991	0.230
LIG_LIR_Gen_1	230	241	PF02991	0.331
LIG_LIR_Gen_1	42	51	PF02991	0.384
LIG_LIR_Nem_3	176	180	PF02991	0.237
LIG_LIR_Nem_3	230	236	PF02991	0.338
LIG_LIR_Nem_3	42	46	PF02991	0.384
LIG_PCNA_yPIPBox_3	16	28	PF02747	0.398
LIG_Pex14_2	233	237	PF04695	0.374
LIG_SH2_SRC	177	180	PF00017	0.416
LIG_SH2_STAP1	69	73	PF00017	0.317
LIG_SH2_STAP1	97	101	PF00017	0.411
LIG_SH2_STAT3	175	178	PF00017	0.411
LIG_SH2_STAT5	175	178	PF00017	0.404
LIG_SH2_STAT5	193	196	PF00017	0.200
LIG_Sin3_3	28	35	PF02671	0.542
LIG_TRAF2_1	176	179	PF00917	0.411
LIG_TRAF2_1	203	206	PF00917	0.230
LIG_UBA3_1	159	165	PF00899	0.230
LIG_UBA3_1	29	36	PF00899	0.401
MOD_CK1_1	229	235	PF00069	0.329
MOD_CK1_1	63	69	PF00069	0.399
MOD_CK1_1	74	80	PF00069	0.352
MOD_CK2_1	173	179	PF00069	0.387
MOD_GlcNHglycan	247	250	PF01048	0.472
MOD_GlcNHglycan	36	39	PF01048	0.625
MOD_GlcNHglycan	73	76	PF01048	0.562
MOD_GSK3_1	213	220	PF00069	0.337
MOD_GSK3_1	225	232	PF00069	0.275
MOD_GSK3_1	73	80	PF00069	0.323
MOD_GSK3_1	84	91	PF00069	0.263
MOD_NEK2_1	125	130	PF00069	0.411
MOD_NEK2_1	159	164	PF00069	0.230
MOD_NEK2_1	213	218	PF00069	0.382
MOD_NEK2_1	257	262	PF00069	0.510
MOD_NEK2_1	45	50	PF00069	0.334
MOD_NEK2_1	84	89	PF00069	0.387
MOD_PIKK_1	60	66	PF00454	0.414
MOD_PIKK_1	77	83	PF00454	0.210
MOD_PK_1	2	8	PF00069	0.495
MOD_PKA_2	1	7	PF00069	0.650
MOD_PKA_2	225	231	PF00069	0.316
MOD_PKA_2	84	90	PF00069	0.372
MOD_PKB_1	224	232	PF00069	0.338
MOD_Plk_1	104	110	PF00069	0.387
MOD_Plk_1	229	235	PF00069	0.355
MOD_Plk_2-3	173	179	PF00069	0.307
MOD_ProDKin_1	74	80	PF00069	0.410
MOD_SUMO_for_1	149	152	PF00179	0.397
MOD_SUMO_rev_2	146	155	PF00179	0.302
MOD_SUMO_rev_2	179	187	PF00179	0.338
TRG_ENDOCYTIC_2	177	180	PF00928	0.230
TRG_ER_diArg_1	223	226	PF00400	0.278
TRG_ER_diLys_1	264	268	PF00400	0.647
TRG_NLS_MonoCore_2	262	267	PF00514	0.695
TRG_NLS_MonoExtC_3	164	170	PF00514	0.338
TRG_NLS_MonoExtN_4	263	268	PF00514	0.708

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7A9	Leptomonas seymouri	56%	100%
A0A1X0P7Z0	Trypanosomatidae	32%	100%
A0A3S7XAW3	Leishmania donovani	81%	100%
A0A422N537	Trypanosoma rangeli	30%	100%
A4IDI9	Leishmania infantum	81%	100%
D0A380	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
E9ASZ2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	82%	100%
P54837	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	28%	100%
Q4Q1K6	Leishmania major	79%	100%
Q5A302	Candida albicans (strain SC5314 / ATCC MYA-2876)	25%	100%
V5BBT5	Trypanosoma cruzi	32%	100%