General transcription factor IIH subunit 4

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

TFIIH basal transcription factor subunit, putative

Species:

Leishmania braziliensis

UniProt:

A4HNU0_LEIBR

TriTrypDb:

LbrM.35.0920 , LBRM2903_350014800

Length:

561

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000439	transcription factor TFIIH core complex	4	12
GO:0005667	transcription regulator complex	2	12
GO:0032991	protein-containing complex	1	12
GO:0090575	RNA polymerase II transcription regulator complex	3	12
GO:0140513	nuclear protein-containing complex	2	12
GO:0005654	nucleoplasm	2	1
GO:0005675	transcription factor TFIIH holo complex	4	1
GO:0032806	carboxy-terminal domain protein kinase complex	3	1
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	1
GO:0110165	cellular anatomical entity	1	1
GO:1902494	catalytic complex	2	1
GO:1902554	serine/threonine protein kinase complex	6	1
GO:1902911	protein kinase complex	5	1
GO:1990234	transferase complex	3	1

Expansion

Sequence features

A4HNU0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HNU0

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006281	DNA repair	5	12
GO:0006289	nucleotide-excision repair	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	12
GO:0006974	DNA damage response	4	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0033554	cellular response to stress	3	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0050896	response to stimulus	1	12
GO:0051716	cellular response to stimulus	2	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006355	regulation of DNA-templated transcription	6	1
GO:0006357	regulation of transcription by RNA polymerase II	7	1
GO:0006468	protein phosphorylation	5	1
GO:0006793	phosphorus metabolic process	3	1
GO:0006796	phosphate-containing compound metabolic process	4	1
GO:0009889	regulation of biosynthetic process	4	1
GO:0010468	regulation of gene expression	5	1
GO:0010556	regulation of macromolecule biosynthetic process	5	1
GO:0016310	phosphorylation	5	1
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	1
GO:0019222	regulation of metabolic process	3	1
GO:0019538	protein metabolic process	3	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031326	regulation of cellular biosynthetic process	5	1
GO:0036211	protein modification process	4	1
GO:0043412	macromolecule modification	4	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051252	regulation of RNA metabolic process	5	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0065007	biological regulation	1	1
GO:0070816	obsolete phosphorylation of RNA polymerase II C-terminal domain	6	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1903506	regulation of nucleic acid-templated transcription	7	1
GO:2001141	regulation of RNA biosynthetic process	6	1

Molecular functions
Term	Name	Level	Count
GO:0001671	ATPase activator activity	3	12
GO:0098772	molecular function regulator activity	1	12
GO:0140677	molecular function activator activity	2	12
GO:0003676	nucleic acid binding	3	1
GO:0003677	DNA binding	4	1
GO:0003690	double-stranded DNA binding	5	1
GO:0005488	binding	1	1
GO:0097159	organic cyclic compound binding	2	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	476	480	PF00656	0.632
CLV_NRD_NRD_1	310	312	PF00675	0.385
CLV_NRD_NRD_1	355	357	PF00675	0.322
CLV_NRD_NRD_1	411	413	PF00675	0.349
CLV_NRD_NRD_1	434	436	PF00675	0.622
CLV_NRD_NRD_1	464	466	PF00675	0.728
CLV_NRD_NRD_1	48	50	PF00675	0.562
CLV_NRD_NRD_1	502	504	PF00675	0.439
CLV_PCSK_KEX2_1	160	162	PF00082	0.526
CLV_PCSK_KEX2_1	310	312	PF00082	0.271
CLV_PCSK_KEX2_1	355	357	PF00082	0.322
CLV_PCSK_KEX2_1	434	436	PF00082	0.588
CLV_PCSK_KEX2_1	464	466	PF00082	0.729
CLV_PCSK_KEX2_1	48	50	PF00082	0.565
CLV_PCSK_KEX2_1	502	504	PF00082	0.425
CLV_PCSK_PC1ET2_1	160	162	PF00082	0.526
CLV_PCSK_SKI1_1	118	122	PF00082	0.528
CLV_PCSK_SKI1_1	160	164	PF00082	0.416
CLV_PCSK_SKI1_1	207	211	PF00082	0.350
CLV_PCSK_SKI1_1	22	26	PF00082	0.398
CLV_PCSK_SKI1_1	235	239	PF00082	0.467
CLV_PCSK_SKI1_1	258	262	PF00082	0.366
CLV_PCSK_SKI1_1	413	417	PF00082	0.406
CLV_PCSK_SKI1_1	503	507	PF00082	0.463
CLV_PCSK_SKI1_1	522	526	PF00082	0.323
DEG_APCC_DBOX_1	117	125	PF00400	0.525
DEG_SPOP_SBC_1	244	248	PF00917	0.310
DEG_SPOP_SBC_1	454	458	PF00917	0.700
DOC_CYCLIN_RxL_1	115	122	PF00134	0.542
DOC_CYCLIN_RxL_1	232	243	PF00134	0.391
DOC_MAPK_FxFP_2	278	281	PF00069	0.394
DOC_MAPK_MEF2A_6	193	200	PF00069	0.321
DOC_MAPK_RevD_3	192	207	PF00069	0.352
DOC_PP1_RVXF_1	256	263	PF00149	0.426
DOC_PP1_RVXF_1	500	507	PF00149	0.448
DOC_PP2B_LxvP_1	484	487	PF13499	0.489
DOC_PP2B_LxvP_1	555	558	PF13499	0.470
DOC_PP2B_LxvP_1	79	82	PF13499	0.646
DOC_PP2B_LxvP_1	8	11	PF13499	0.578
DOC_PP2B_PxIxI_1	10	16	PF00149	0.280
DOC_PP4_FxxP_1	132	135	PF00568	0.611
DOC_PP4_FxxP_1	278	281	PF00568	0.394
DOC_PP4_FxxP_1	459	462	PF00568	0.546
DOC_USP7_MATH_1	428	432	PF00917	0.321
DOC_USP7_MATH_1	68	72	PF00917	0.483
DOC_USP7_MATH_1	82	86	PF00917	0.501
DOC_WW_Pin1_4	207	212	PF00397	0.394
DOC_WW_Pin1_4	313	318	PF00397	0.310
DOC_WW_Pin1_4	444	449	PF00397	0.739
LIG_14-3-3_CanoR_1	102	106	PF00244	0.533
LIG_14-3-3_CanoR_1	118	128	PF00244	0.561
LIG_14-3-3_CanoR_1	154	163	PF00244	0.478
LIG_14-3-3_CanoR_1	164	171	PF00244	0.403
LIG_14-3-3_CanoR_1	241	245	PF00244	0.449
LIG_14-3-3_CanoR_1	412	421	PF00244	0.327
LIG_14-3-3_CanoR_1	422	427	PF00244	0.334
LIG_14-3-3_CanoR_1	472	478	PF00244	0.551
LIG_14-3-3_CanoR_1	514	524	PF00244	0.513
LIG_Actin_WH2_2	149	166	PF00022	0.460
LIG_BIR_II_1	1	5	PF00653	0.609
LIG_BIR_III_4	176	180	PF00653	0.348
LIG_BRCT_BRCA1_1	158	162	PF00533	0.517
LIG_BRCT_BRCA1_1	315	319	PF00533	0.334
LIG_BRCT_BRCA1_1	331	335	PF00533	0.322
LIG_BRCT_BRCA1_1	455	459	PF00533	0.738
LIG_BRCT_BRCA1_1	473	477	PF00533	0.357
LIG_BRCT_BRCA1_1	72	76	PF00533	0.556
LIG_BRCT_BRCA1_1	93	97	PF00533	0.441
LIG_CaM_IQ_9	41	57	PF13499	0.649
LIG_FHA_1	166	172	PF00498	0.463
LIG_FHA_1	255	261	PF00498	0.409
LIG_FHA_1	357	363	PF00498	0.413
LIG_FHA_1	416	422	PF00498	0.335
LIG_FHA_1	473	479	PF00498	0.664
LIG_FHA_1	480	486	PF00498	0.535
LIG_FHA_1	523	529	PF00498	0.534
LIG_FHA_2	474	480	PF00498	0.680
LIG_LIR_Apic_2	106	112	PF02991	0.567
LIG_LIR_Apic_2	456	462	PF02991	0.545
LIG_LIR_Gen_1	215	221	PF02991	0.453
LIG_LIR_Gen_1	541	552	PF02991	0.416
LIG_LIR_Gen_1	70	80	PF02991	0.587
LIG_LIR_Gen_1	9	20	PF02991	0.323
LIG_LIR_LC3C_4	482	486	PF02991	0.523
LIG_LIR_Nem_3	215	219	PF02991	0.453
LIG_LIR_Nem_3	332	338	PF02991	0.200
LIG_LIR_Nem_3	365	371	PF02991	0.295
LIG_LIR_Nem_3	541	547	PF02991	0.420
LIG_LIR_Nem_3	549	555	PF02991	0.387
LIG_LIR_Nem_3	70	75	PF02991	0.534
LIG_LIR_Nem_3	9	15	PF02991	0.318
LIG_LIR_Nem_3	95	101	PF02991	0.357
LIG_LYPXL_S_1	302	306	PF13949	0.322
LIG_LYPXL_yS_3	303	306	PF13949	0.322
LIG_MLH1_MIPbox_1	93	97	PF16413	0.343
LIG_NRBOX	260	266	PF00104	0.311
LIG_NRBOX	62	68	PF00104	0.602
LIG_PCNA_PIPBox_1	521	530	PF02747	0.547
LIG_PCNA_yPIPBox_3	181	189	PF02747	0.486
LIG_PCNA_yPIPBox_3	224	237	PF02747	0.453
LIG_PCNA_yPIPBox_3	514	528	PF02747	0.546
LIG_Pex14_2	72	76	PF04695	0.465
LIG_SH2_CRK	544	548	PF00017	0.439
LIG_SH2_SRC	37	40	PF00017	0.508
LIG_SH2_STAP1	37	41	PF00017	0.479
LIG_SH2_STAT5	276	279	PF00017	0.394
LIG_SH2_STAT5	338	341	PF00017	0.369
LIG_SH2_STAT5	373	376	PF00017	0.306
LIG_SH2_STAT5	527	530	PF00017	0.549
LIG_SH2_STAT5	96	99	PF00017	0.460
LIG_SH3_1	193	199	PF00018	0.380
LIG_SH3_3	143	149	PF00018	0.557
LIG_SH3_3	193	199	PF00018	0.496
LIG_SH3_3	253	259	PF00018	0.414
LIG_SH3_3	311	317	PF00018	0.363
LIG_SUMO_SIM_anti_2	234	240	PF11976	0.372
LIG_SUMO_SIM_par_1	148	153	PF11976	0.547
LIG_SUMO_SIM_par_1	234	240	PF11976	0.363
LIG_SUMO_SIM_par_1	263	269	PF11976	0.453
LIG_SUMO_SIM_par_1	32	38	PF11976	0.530
LIG_TRFH_1	145	149	PF08558	0.567
LIG_TYR_ITIM	301	306	PF00017	0.363
LIG_TYR_ITIM	35	40	PF00017	0.464
LIG_WRC_WIRS_1	69	74	PF05994	0.349
LIG_WW_3	461	465	PF00397	0.502
MOD_CK1_1	2	8	PF00069	0.641
MOD_CK1_1	243	249	PF00069	0.423
MOD_CK1_1	251	257	PF00069	0.400
MOD_CK1_1	349	355	PF00069	0.427
MOD_CK1_1	457	463	PF00069	0.702
MOD_CK1_1	70	76	PF00069	0.370
MOD_CK2_1	68	74	PF00069	0.473
MOD_Cter_Amidation	46	49	PF01082	0.547
MOD_GlcNHglycan	172	176	PF01048	0.450
MOD_GlcNHglycan	250	253	PF01048	0.368
MOD_GlcNHglycan	341	344	PF01048	0.353
MOD_GlcNHglycan	351	354	PF01048	0.403
MOD_GlcNHglycan	459	462	PF01048	0.641
MOD_GSK3_1	240	247	PF00069	0.388
MOD_GSK3_1	250	257	PF00069	0.438
MOD_GSK3_1	266	273	PF00069	0.149
MOD_GSK3_1	442	449	PF00069	0.659
MOD_GSK3_1	453	460	PF00069	0.692
MOD_GSK3_1	468	475	PF00069	0.577
MOD_N-GLC_1	244	249	PF02516	0.406
MOD_N-GLC_1	346	351	PF02516	0.200
MOD_N-GLC_1	514	519	PF02516	0.440
MOD_NEK2_1	1	6	PF00069	0.608
MOD_NEK2_1	156	161	PF00069	0.416
MOD_NEK2_1	162	167	PF00069	0.397
MOD_NEK2_1	237	242	PF00069	0.334
MOD_NEK2_1	331	336	PF00069	0.352
MOD_NEK2_1	339	344	PF00069	0.323
MOD_NEK2_1	346	351	PF00069	0.250
MOD_NEK2_1	54	59	PF00069	0.633
MOD_NEK2_1	67	72	PF00069	0.465
MOD_PIKK_1	107	113	PF00454	0.408
MOD_PIKK_1	119	125	PF00454	0.489
MOD_PIKK_1	415	421	PF00454	0.335
MOD_PIKK_1	80	86	PF00454	0.691
MOD_PKA_2	101	107	PF00069	0.494
MOD_PKA_2	240	246	PF00069	0.323
MOD_PKA_2	254	260	PF00069	0.223
MOD_PKA_2	349	355	PF00069	0.401
MOD_PKA_2	400	406	PF00069	0.315
MOD_PKA_2	471	477	PF00069	0.580
MOD_PKA_2	54	60	PF00069	0.687
MOD_Plk_1	346	352	PF00069	0.260
MOD_Plk_4	266	272	PF00069	0.442
MOD_Plk_4	3	9	PF00069	0.398
MOD_Plk_4	358	364	PF00069	0.306
MOD_Plk_4	428	434	PF00069	0.445
MOD_Plk_4	473	479	PF00069	0.612
MOD_Plk_4	480	486	PF00069	0.517
MOD_Plk_4	92	98	PF00069	0.506
MOD_ProDKin_1	207	213	PF00069	0.394
MOD_ProDKin_1	313	319	PF00069	0.310
MOD_ProDKin_1	444	450	PF00069	0.738
MOD_SUMO_for_1	205	208	PF00179	0.371
MOD_SUMO_rev_2	110	117	PF00179	0.357
MOD_SUMO_rev_2	438	448	PF00179	0.569
TRG_DiLeu_BaEn_1	234	239	PF01217	0.453
TRG_DiLeu_BaEn_1	28	33	PF01217	0.411
TRG_DiLeu_BaEn_1	375	380	PF01217	0.200
TRG_DiLeu_BaLyEn_6	256	261	PF01217	0.414
TRG_DiLeu_BaLyEn_6	551	556	PF01217	0.392
TRG_ENDOCYTIC_2	303	306	PF00928	0.322
TRG_ENDOCYTIC_2	37	40	PF00928	0.423
TRG_ENDOCYTIC_2	544	547	PF00928	0.387
TRG_ER_diArg_1	354	356	PF00400	0.325
TRG_ER_diArg_1	433	435	PF00400	0.592
TRG_ER_diArg_1	463	465	PF00400	0.717
TRG_ER_diArg_1	502	504	PF00400	0.440
TRG_Pf-PMV_PEXEL_1	118	123	PF00026	0.527

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7V5	Leptomonas seymouri	65%	100%
A0A0S4JS94	Bodo saltans	31%	100%
A0A1X0P966	Trypanosomatidae	42%	100%
A0A3S5H816	Leishmania donovani	81%	100%
A0A422NK89	Trypanosoma rangeli	44%	100%
A4ICQ4	Leishmania infantum	81%	100%
D0A2Q5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E9ASK0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	82%	100%
Q02939	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	100%
Q4Q1Z6	Leishmania major	83%	100%
V5B9Y1	Trypanosoma cruzi	42%	100%