Ribonuclease

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Ribonuclease

Gene product:

ribonuclease H, putative

Species:

Leishmania braziliensis

UniProt:

A4HNS4_LEIBR

TriTrypDb:

LbrM.35.0760 , LBRM2903_350012900

Length:

318

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0032299	ribonuclease H2 complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0110165	cellular anatomical entity	1	1
GO:0140535	intracellular protein-containing complex	2	1
GO:1902494	catalytic complex	2	1

Expansion

Sequence features

A4HNS4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HNS4

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006259	DNA metabolic process	4	1
GO:0006281	DNA repair	5	1
GO:0006298	mismatch repair	6	1
GO:0006401	RNA catabolic process	5	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0006950	response to stress	2	1
GO:0006974	DNA damage response	4	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0019439	aromatic compound catabolic process	4	1
GO:0033554	cellular response to stress	3	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034655	nucleobase-containing compound catabolic process	4	1
GO:0043137	DNA replication, removal of RNA primer	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0044270	cellular nitrogen compound catabolic process	4	1
GO:0046483	heterocycle metabolic process	3	1
GO:0046700	heterocycle catabolic process	4	1
GO:0050896	response to stimulus	1	1
GO:0051716	cellular response to stimulus	2	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901361	organic cyclic compound catabolic process	4	1
GO:1901575	organic substance catabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0003824	catalytic activity	1	11
GO:0004518	nuclease activity	4	11
GO:0004519	endonuclease activity	5	11
GO:0004521	RNA endonuclease activity	5	11
GO:0004523	RNA-DNA hybrid ribonuclease activity	7	11
GO:0004540	RNA nuclease activity	4	11
GO:0005488	binding	1	11
GO:0016787	hydrolase activity	2	11
GO:0016788	hydrolase activity, acting on ester bonds	3	11
GO:0016891	RNA endonuclease activity, producing 5'-phosphomonoesters	6	11
GO:0016893	endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	11
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0097159	organic cyclic compound binding	2	11
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	20	22	PF00675	0.475
CLV_NRD_NRD_1	278	280	PF00675	0.425
CLV_PCSK_KEX2_1	20	22	PF00082	0.604
CLV_PCSK_KEX2_1	278	280	PF00082	0.404
CLV_PCSK_KEX2_1	299	301	PF00082	0.512
CLV_PCSK_PC1ET2_1	299	301	PF00082	0.624
CLV_PCSK_SKI1_1	101	105	PF00082	0.271
CLV_PCSK_SKI1_1	107	111	PF00082	0.266
DEG_APCC_DBOX_1	90	98	PF00400	0.555
DOC_ANK_TNKS_1	53	60	PF00023	0.503
DOC_MAPK_gen_1	104	113	PF00069	0.482
DOC_MAPK_gen_1	20	29	PF00069	0.517
DOC_MAPK_MEF2A_6	174	182	PF00069	0.451
DOC_MAPK_MEF2A_6	247	256	PF00069	0.513
DOC_PP1_RVXF_1	222	229	PF00149	0.374
DOC_PP1_RVXF_1	288	294	PF00149	0.577
DOC_PP4_FxxP_1	293	296	PF00568	0.569
DOC_SPAK_OSR1_1	235	239	PF12202	0.432
DOC_USP7_MATH_1	10	14	PF00917	0.643
DOC_USP7_MATH_1	191	195	PF00917	0.459
DOC_USP7_MATH_1	22	26	PF00917	0.601
DOC_USP7_MATH_2	115	121	PF00917	0.461
DOC_USP7_UBL2_3	185	189	PF12436	0.390
DOC_USP7_UBL2_3	295	299	PF12436	0.650
DOC_WW_Pin1_4	1	6	PF00397	0.697
DOC_WW_Pin1_4	111	116	PF00397	0.444
LIG_14-3-3_CanoR_1	101	107	PF00244	0.457
LIG_14-3-3_CanoR_1	128	134	PF00244	0.527
LIG_14-3-3_CanoR_1	179	183	PF00244	0.451
LIG_14-3-3_CanoR_1	20	29	PF00244	0.531
LIG_14-3-3_CanoR_1	247	251	PF00244	0.408
LIG_14-3-3_CanoR_1	93	98	PF00244	0.488
LIG_DLG_GKlike_1	93	100	PF00625	0.531
LIG_EH1_1	28	36	PF00400	0.544
LIG_FHA_1	108	114	PF00498	0.455
LIG_FHA_1	22	28	PF00498	0.595
LIG_FHA_1	303	309	PF00498	0.467
LIG_FHA_1	312	318	PF00498	0.481
LIG_FHA_1	62	68	PF00498	0.531
LIG_FHA_2	200	206	PF00498	0.531
LIG_LIR_Apic_2	292	296	PF02991	0.569
LIG_LIR_Gen_1	42	52	PF02991	0.407
LIG_LIR_Nem_3	105	109	PF02991	0.451
LIG_LIR_Nem_3	187	193	PF02991	0.523
LIG_LIR_Nem_3	249	253	PF02991	0.475
LIG_LIR_Nem_3	42	47	PF02991	0.470
LIG_REV1ctd_RIR_1	234	244	PF16727	0.461
LIG_SH2_CRK	243	247	PF00017	0.519
LIG_SH2_STAP1	304	308	PF00017	0.486
LIG_SH2_STAT5	157	160	PF00017	0.451
LIG_SH2_STAT5	245	248	PF00017	0.374
LIG_SH2_STAT5	304	307	PF00017	0.482
LIG_SH2_STAT5	44	47	PF00017	0.448
LIG_SH2_STAT5	63	66	PF00017	0.332
LIG_SH3_3	158	164	PF00018	0.424
LIG_SH3_3	217	223	PF00018	0.444
LIG_SH3_3	259	265	PF00018	0.444
LIG_SH3_3	54	60	PF00018	0.465
LIG_SH3_4	295	302	PF00018	0.643
LIG_SUMO_SIM_par_1	66	72	PF11976	0.451
LIG_TRAF2_1	114	117	PF00917	0.531
LIG_TRAF2_1	207	210	PF00917	0.374
LIG_UBA3_1	307	311	PF00899	0.487
LIG_WRC_WIRS_1	103	108	PF05994	0.304
MOD_CK1_1	120	126	PF00069	0.307
MOD_CK1_1	134	140	PF00069	0.302
MOD_CK1_1	143	149	PF00069	0.304
MOD_CK1_1	194	200	PF00069	0.416
MOD_CK1_1	6	12	PF00069	0.575
MOD_CK2_1	111	117	PF00069	0.304
MOD_CK2_1	22	28	PF00069	0.540
MOD_CK2_1	312	318	PF00069	0.520
MOD_CK2_1	36	42	PF00069	0.372
MOD_CK2_1	66	72	PF00069	0.409
MOD_GlcNHglycan	12	15	PF01048	0.590
MOD_GlcNHglycan	125	128	PF01048	0.410
MOD_GlcNHglycan	148	151	PF01048	0.377
MOD_GlcNHglycan	16	19	PF01048	0.545
MOD_GlcNHglycan	193	196	PF01048	0.416
MOD_GlcNHglycan	216	219	PF01048	0.267
MOD_GlcNHglycan	5	8	PF01048	0.593
MOD_GSK3_1	107	114	PF00069	0.304
MOD_GSK3_1	116	123	PF00069	0.304
MOD_GSK3_1	199	206	PF00069	0.369
MOD_GSK3_1	214	221	PF00069	0.198
MOD_GSK3_1	6	13	PF00069	0.585
MOD_NEK2_1	178	183	PF00069	0.304
MOD_NEK2_1	302	307	PF00069	0.499
MOD_NEK2_1	61	66	PF00069	0.267
MOD_PKA_2	10	16	PF00069	0.640
MOD_PKA_2	178	184	PF00069	0.331
MOD_PKA_2	203	209	PF00069	0.313
MOD_PKA_2	22	28	PF00069	0.538
MOD_PKA_2	246	252	PF00069	0.304
MOD_PKB_1	91	99	PF00069	0.416
MOD_Plk_1	143	149	PF00069	0.410
MOD_Plk_2-3	36	42	PF00069	0.280
MOD_Plk_4	194	200	PF00069	0.292
MOD_Plk_4	22	28	PF00069	0.664
MOD_Plk_4	312	318	PF00069	0.587
MOD_ProDKin_1	1	7	PF00069	0.693
MOD_ProDKin_1	111	117	PF00069	0.294
TRG_DiLeu_BaEn_1	72	77	PF01217	0.304
TRG_ENDOCYTIC_2	243	246	PF00928	0.399
TRG_ENDOCYTIC_2	304	307	PF00928	0.482
TRG_ENDOCYTIC_2	44	47	PF00928	0.461
TRG_ER_diArg_1	20	23	PF00400	0.476
TRG_ER_diArg_1	278	280	PF00400	0.514
TRG_ER_diArg_1	91	94	PF00400	0.416
TRG_Pf-PMV_PEXEL_1	86	90	PF00026	0.294

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7W5	Leptomonas seymouri	80%	100%
A0A1X0P866	Trypanosomatidae	59%	96%
A0A3R7RS79	Trypanosoma rangeli	62%	98%
A0A3S7XAC0	Leishmania donovani	81%	100%
A2BL34	Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)	40%	100%
A4FWE5	Methanococcus maripaludis (strain C5 / ATCC BAA-1333)	33%	100%
A4ICS0	Leishmania infantum	82%	100%
A6UQ18	Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB)	32%	100%
A6VGW0	Methanococcus maripaludis (strain C7 / ATCC BAA-1331)	32%	100%
A9A9T9	Methanococcus maripaludis (strain C6 / ATCC BAA-1332)	32%	100%
B6YVT5	Thermococcus onnurineus (strain NA1)	36%	100%
C6A1I4	Thermococcus sibiricus (strain DSM 12597 / MM 739)	35%	100%
D0A2N4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	59%	98%
E9ASI4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	79%	100%
O59351	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	34%	100%
O74035	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	36%	100%
O75792	Homo sapiens	45%	100%
P53942	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	38%	100%
Q0W5R8	Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50)	35%	100%
Q10236	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	40%	98%
Q12YN9	Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)	33%	100%
Q2TBT5	Bos taurus	46%	100%
Q3IRF6	Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)	33%	100%
Q468N6	Methanosarcina barkeri (strain Fusaro / DSM 804)	31%	100%
Q54D13	Dictyostelium discoideum	35%	100%
Q5U209	Rattus norvegicus	47%	100%
Q6LXH7	Methanococcus maripaludis (strain S2 / LL)	32%	100%
Q8ISS8	Leishmania major	86%	99%
Q8PTA3	Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)	32%	100%
Q8TPF4	Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)	32%	100%
Q8U036	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	35%	100%
Q97CG6	Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)	33%	100%
Q9CWY8	Mus musculus	45%	100%
Q9P5X8	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	36%	100%
Q9SEZ6	Arabidopsis thaliana	42%	100%
Q9U6P6	Caenorhabditis elegans	41%	100%
Q9VPP5	Drosophila melanogaster	38%	92%
Q9YET5	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	35%	100%
V5BQG7	Trypanosoma cruzi	59%	98%