mRNA (guanine-N(7))-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cap guanine-N7 methyltransferase

Species:

Leishmania braziliensis

UniProt:

A4HNL9_LEIBR

TriTrypDb:

LbrM.35.0190 , LBRM2903_350007400

Length:

323

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005845	mRNA cap binding complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0034518	RNA cap binding complex	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	2
GO:0140535	intracellular protein-containing complex	2	1
GO:0016020	membrane	2	1

Expansion

Sequence features

A4HNL9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HNL9

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006370	7-methylguanosine mRNA capping	8	1
GO:0006396	RNA processing	6	1
GO:0006397	mRNA processing	7	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009452	7-methylguanosine RNA capping	8	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0016071	mRNA metabolic process	6	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0036260	RNA capping	7	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0004482	mRNA (guanine-N7-)-methyltransferase activity	6	12
GO:0005488	binding	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008170	N-methyltransferase activity	5	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008174	mRNA methyltransferase activity	5	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	228	232	PF00656	0.171
CLV_NRD_NRD_1	113	115	PF00675	0.311
CLV_NRD_NRD_1	14	16	PF00675	0.430
CLV_PCSK_KEX2_1	113	115	PF00082	0.284
CLV_PCSK_KEX2_1	16	18	PF00082	0.284
CLV_PCSK_KEX2_1	204	206	PF00082	0.391
CLV_PCSK_PC1ET2_1	16	18	PF00082	0.308
CLV_PCSK_PC1ET2_1	204	206	PF00082	0.380
CLV_PCSK_SKI1_1	176	180	PF00082	0.342
DEG_ODPH_VHL_1	186	199	PF01847	0.321
DOC_CYCLIN_RxL_1	166	177	PF00134	0.296
DOC_CYCLIN_RxL_1	309	317	PF00134	0.362
DOC_MAPK_gen_1	36	42	PF00069	0.306
DOC_MAPK_MEF2A_6	155	163	PF00069	0.283
DOC_PP1_RVXF_1	310	317	PF00149	0.326
DOC_PP4_FxxP_1	220	223	PF00568	0.342
DOC_PP4_FxxP_1	316	319	PF00568	0.208
DOC_USP7_MATH_1	168	172	PF00917	0.336
DOC_USP7_MATH_1	181	185	PF00917	0.277
DOC_USP7_MATH_1	53	57	PF00917	0.321
DOC_USP7_MATH_1	89	93	PF00917	0.226
DOC_WW_Pin1_4	101	106	PF00397	0.199
DOC_WW_Pin1_4	147	152	PF00397	0.392
LIG_14-3-3_CanoR_1	127	133	PF00244	0.292
LIG_14-3-3_CanoR_1	299	307	PF00244	0.285
LIG_BRCT_BRCA1_1	138	142	PF00533	0.234
LIG_BRCT_BRCA1_1	149	153	PF00533	0.307
LIG_BRCT_BRCA1_1	159	163	PF00533	0.283
LIG_BRCT_BRCA1_1	216	220	PF00533	0.350
LIG_BRCT_BRCA1_1	277	281	PF00533	0.283
LIG_deltaCOP1_diTrp_1	19	28	PF00928	0.395
LIG_EVH1_1	220	224	PF00568	0.392
LIG_FHA_1	194	200	PF00498	0.439
LIG_FHA_1	232	238	PF00498	0.330
LIG_FHA_2	131	137	PF00498	0.308
LIG_FHA_2	193	199	PF00498	0.263
LIG_FHA_2	226	232	PF00498	0.238
LIG_FHA_2	236	242	PF00498	0.293
LIG_FHA_2	94	100	PF00498	0.243
LIG_LIR_Apic_2	217	223	PF02991	0.350
LIG_LIR_Apic_2	254	258	PF02991	0.267
LIG_LIR_Apic_2	313	319	PF02991	0.197
LIG_LIR_Gen_1	177	187	PF02991	0.296
LIG_LIR_Gen_1	225	231	PF02991	0.292
LIG_LIR_Gen_1	278	288	PF02991	0.308
LIG_LIR_Nem_3	150	156	PF02991	0.313
LIG_LIR_Nem_3	177	182	PF02991	0.296
LIG_LIR_Nem_3	225	229	PF02991	0.292
LIG_LIR_Nem_3	278	284	PF02991	0.290
LIG_LIR_Nem_3	309	314	PF02991	0.275
LIG_LIR_Nem_3	95	101	PF02991	0.344
LIG_PCNA_yPIPBox_3	36	48	PF02747	0.321
LIG_Pex14_2	138	142	PF04695	0.248
LIG_PTB_Apo_2	210	217	PF02174	0.392
LIG_PTB_Phospho_1	210	216	PF10480	0.392
LIG_SH2_CRK	156	160	PF00017	0.392
LIG_SH2_CRK	226	230	PF00017	0.292
LIG_SH2_CRK	259	263	PF00017	0.196
LIG_SH2_CRK	311	315	PF00017	0.300
LIG_SH2_NCK_1	10	14	PF00017	0.398
LIG_SH2_NCK_1	156	160	PF00017	0.321
LIG_SH2_NCK_1	226	230	PF00017	0.246
LIG_SH2_PTP2	255	258	PF00017	0.283
LIG_SH2_SRC	10	13	PF00017	0.402
LIG_SH2_SRC	101	104	PF00017	0.283
LIG_SH2_STAP1	216	220	PF00017	0.418
LIG_SH2_STAP1	259	263	PF00017	0.446
LIG_SH2_STAP1	288	292	PF00017	0.367
LIG_SH2_STAT5	226	229	PF00017	0.392
LIG_SH2_STAT5	255	258	PF00017	0.258
LIG_SH3_3	145	151	PF00018	0.392
LIG_SH3_3	218	224	PF00018	0.392
LIG_SH3_3	247	253	PF00018	0.271
LIG_SUMO_SIM_anti_2	171	177	PF11976	0.321
LIG_SUMO_SIM_anti_2	304	309	PF11976	0.365
LIG_SUMO_SIM_par_1	190	196	PF11976	0.392
LIG_SUMO_SIM_par_1	233	238	PF11976	0.385
LIG_SUMO_SIM_par_1	60	66	PF11976	0.248
LIG_TYR_ITIM	257	262	PF00017	0.196
LIG_WW_1	223	226	PF00397	0.321
MOD_CK1_1	180	186	PF00069	0.392
MOD_CK1_1	230	236	PF00069	0.413
MOD_CK1_1	92	98	PF00069	0.283
MOD_CK2_1	106	112	PF00069	0.446
MOD_CK2_1	235	241	PF00069	0.321
MOD_CK2_1	298	304	PF00069	0.392
MOD_CK2_1	51	57	PF00069	0.321
MOD_GlcNHglycan	179	182	PF01048	0.392
MOD_GlcNHglycan	183	186	PF01048	0.392
MOD_GlcNHglycan	288	291	PF01048	0.399
MOD_GlcNHglycan	53	56	PF01048	0.250
MOD_GlcNHglycan	65	68	PF01048	0.254
MOD_GlcNHglycan	80	83	PF01048	0.248
MOD_GSK3_1	143	150	PF00069	0.392
MOD_GSK3_1	177	184	PF00069	0.392
MOD_GSK3_1	227	234	PF00069	0.332
MOD_GSK3_1	275	282	PF00069	0.367
MOD_GSK3_1	306	313	PF00069	0.221
MOD_GSK3_1	89	96	PF00069	0.363
MOD_NEK2_1	138	143	PF00069	0.342
MOD_NEK2_1	273	278	PF00069	0.263
MOD_NEK2_1	310	315	PF00069	0.182
MOD_NEK2_1	78	83	PF00069	0.308
MOD_NEK2_1	93	98	PF00069	0.389
MOD_NEK2_2	89	94	PF00069	0.171
MOD_PIKK_1	20	26	PF00454	0.392
MOD_PIKK_1	298	304	PF00454	0.246
MOD_PKA_2	123	129	PF00069	0.263
MOD_PKA_2	143	149	PF00069	0.446
MOD_PKA_2	298	304	PF00069	0.176
MOD_Plk_1	224	230	PF00069	0.171
MOD_Plk_2-3	106	112	PF00069	0.171
MOD_Plk_2-3	225	231	PF00069	0.171
MOD_Plk_4	138	144	PF00069	0.343
MOD_Plk_4	231	237	PF00069	0.196
MOD_Plk_4	306	312	PF00069	0.320
MOD_Plk_4	35	41	PF00069	0.226
MOD_Plk_4	93	99	PF00069	0.392
MOD_ProDKin_1	101	107	PF00069	0.199
MOD_ProDKin_1	147	153	PF00069	0.392
TRG_ENDOCYTIC_2	10	13	PF00928	0.368
TRG_ENDOCYTIC_2	156	159	PF00928	0.348
TRG_ENDOCYTIC_2	226	229	PF00928	0.278
TRG_ENDOCYTIC_2	259	262	PF00928	0.309
TRG_ENDOCYTIC_2	311	314	PF00928	0.267
TRG_Pf-PMV_PEXEL_1	312	317	PF00026	0.402

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3T1	Leptomonas seymouri	60%	100%
A0A0S4JM54	Bodo saltans	40%	93%
A0A1X0P9E2	Trypanosomatidae	46%	100%
A0A3Q8IQD0	Leishmania donovani	81%	100%
A0A3R7LST7	Trypanosoma rangeli	48%	100%
A4ICX5	Leishmania infantum	81%	100%
A4R8D7	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	26%	66%
D0A2H4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	45%	91%
E9ASD0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	82%	100%
O43148	Homo sapiens	27%	68%
O74880	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	90%
P32783	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	28%	74%
Q1MTD3	Danio rerio	30%	81%
Q28FT4	Xenopus tropicalis	27%	80%
Q4Q266	Leishmania major	81%	100%
Q4R7K1	Macaca fascicularis	27%	68%
Q5ADX5	Candida albicans (strain SC5314 / ATCC MYA-2876)	26%	68%
Q5U2U7	Rattus norvegicus	28%	70%
Q61E36	Caenorhabditis briggsae	29%	85%
Q6CKI0	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	28%	76%
Q6FML4	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	28%	77%
Q6Z9U7	Oryza sativa subsp. japonica	30%	88%
Q754U7	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	27%	75%
Q8SR66	Encephalitozoon cuniculi (strain GB-M1)	24%	100%
Q9D0L8	Mus musculus	28%	69%
Q9I8S2	Xenopus laevis	27%	80%
Q9LHQ7	Arabidopsis thaliana	30%	87%
Q9VJQ4	Drosophila melanogaster	27%	76%
Q9XVS1	Caenorhabditis elegans	29%	85%
V5BA31	Trypanosoma cruzi	44%	100%