Putative PUF1

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Putative PUF1

Gene product:

PUF1, putative

Species:

Leishmania braziliensis

UniProt:

A4HNL0_LEIBR

TriTrypDb:

LbrM.35.0090 , LBRM2903_350006300

Length:

551

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 17
NetGPI	no	yes: 0, no: 18

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	2
GO:0016020	membrane	2	1

Expansion

Sequence features

A4HNL0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HNL0

Function

Biological processes
Term	Name	Level	Count
GO:0010468	regulation of gene expression	5	1
GO:0010608	post-transcriptional regulation of gene expression	6	1
GO:0019222	regulation of metabolic process	3	1
GO:0050789	regulation of biological process	2	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0065007	biological regulation	1	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	19
GO:0003723	RNA binding	4	19
GO:0005488	binding	1	19
GO:0097159	organic cyclic compound binding	2	19
GO:1901363	heterocyclic compound binding	2	19
GO:0003729	mRNA binding	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	13	15	PF00675	0.401
CLV_NRD_NRD_1	441	443	PF00675	0.418
CLV_NRD_NRD_1	448	450	PF00675	0.470
CLV_PCSK_KEX2_1	13	15	PF00082	0.407
CLV_PCSK_KEX2_1	44	46	PF00082	0.548
CLV_PCSK_KEX2_1	441	443	PF00082	0.390
CLV_PCSK_KEX2_1	448	450	PF00082	0.445
CLV_PCSK_PC1ET2_1	44	46	PF00082	0.625
CLV_PCSK_PC7_1	444	450	PF00082	0.551
CLV_PCSK_SKI1_1	104	108	PF00082	0.330
CLV_PCSK_SKI1_1	113	117	PF00082	0.380
CLV_PCSK_SKI1_1	224	228	PF00082	0.408
CLV_PCSK_SKI1_1	342	346	PF00082	0.326
CLV_PCSK_SKI1_1	363	367	PF00082	0.246
CLV_PCSK_SKI1_1	373	377	PF00082	0.210
CLV_PCSK_SKI1_1	388	392	PF00082	0.320
CLV_PCSK_SKI1_1	448	452	PF00082	0.608
CLV_PCSK_SKI1_1	75	79	PF00082	0.455
DEG_APCC_DBOX_1	341	349	PF00400	0.320
DEG_APCC_DBOX_1	74	82	PF00400	0.451
DOC_PP2B_LxvP_1	380	383	PF13499	0.307
DOC_PP4_FxxP_1	508	511	PF00568	0.676
DOC_USP7_MATH_1	122	126	PF00917	0.351
DOC_USP7_MATH_1	182	186	PF00917	0.398
DOC_USP7_MATH_1	239	243	PF00917	0.504
DOC_USP7_MATH_1	421	425	PF00917	0.462
DOC_USP7_UBL2_3	434	438	PF12436	0.356
DOC_WW_Pin1_4	366	371	PF00397	0.298
DOC_WW_Pin1_4	426	431	PF00397	0.346
DOC_WW_Pin1_4	530	535	PF00397	0.422
LIG_14-3-3_CanoR_1	136	144	PF00244	0.473
LIG_14-3-3_CanoR_1	183	189	PF00244	0.348
LIG_Actin_WH2_2	284	299	PF00022	0.301
LIG_Actin_WH2_2	353	368	PF00022	0.252
LIG_Actin_WH2_2	412	429	PF00022	0.369
LIG_Actin_WH2_2	61	77	PF00022	0.347
LIG_AP2alpha_2	301	303	PF02296	0.252
LIG_BIR_II_1	1	5	PF00653	0.537
LIG_BIR_III_4	121	125	PF00653	0.418
LIG_BIR_III_4	70	74	PF00653	0.385
LIG_BRCT_BRCA1_1	227	231	PF00533	0.419
LIG_CaM_IQ_9	84	100	PF13499	0.329
LIG_FHA_1	200	206	PF00498	0.429
LIG_FHA_1	283	289	PF00498	0.295
LIG_FHA_1	335	341	PF00498	0.363
LIG_FHA_2	141	147	PF00498	0.523
LIG_FHA_2	192	198	PF00498	0.333
LIG_FHA_2	201	207	PF00498	0.380
LIG_FHA_2	403	409	PF00498	0.291
LIG_FHA_2	44	50	PF00498	0.546
LIG_IRF3_LxIS_1	161	166	PF10401	0.169
LIG_LIR_Apic_2	507	511	PF02991	0.519
LIG_LIR_Gen_1	199	209	PF02991	0.400
LIG_LIR_Gen_1	228	239	PF02991	0.458
LIG_LIR_Gen_1	249	260	PF02991	0.295
LIG_LIR_Nem_3	196	201	PF02991	0.385
LIG_LIR_Nem_3	228	234	PF02991	0.424
LIG_LIR_Nem_3	249	255	PF02991	0.320
LIG_LIR_Nem_3	301	306	PF02991	0.282
LIG_LIR_Nem_3	391	397	PF02991	0.245
LIG_PCNA_yPIPBox_3	214	226	PF02747	0.318
LIG_Pex14_2	103	107	PF04695	0.329
LIG_PTAP_UEV_1	330	335	PF05743	0.382
LIG_PTB_Apo_2	425	432	PF02174	0.349
LIG_PTB_Phospho_1	425	431	PF10480	0.354
LIG_Rb_LxCxE_1	23	41	PF01857	0.289
LIG_SH2_CRK	201	205	PF00017	0.421
LIG_SH2_CRK	474	478	PF00017	0.694
LIG_SH2_GRB2like	336	339	PF00017	0.331
LIG_SH2_NCK_1	248	252	PF00017	0.332
LIG_SH2_STAP1	201	205	PF00017	0.421
LIG_SH2_STAP1	248	252	PF00017	0.375
LIG_SH2_STAP1	336	340	PF00017	0.359
LIG_SH2_STAT3	306	309	PF00017	0.245
LIG_SH2_STAT3	336	339	PF00017	0.424
LIG_SH2_STAT3	397	400	PF00017	0.245
LIG_SH2_STAT5	201	204	PF00017	0.388
LIG_SH2_STAT5	306	309	PF00017	0.276
LIG_SH2_STAT5	336	339	PF00017	0.331
LIG_SH2_STAT5	397	400	PF00017	0.248
LIG_SH2_STAT5	86	89	PF00017	0.461
LIG_SH3_3	296	302	PF00018	0.277
LIG_SH3_3	328	334	PF00018	0.411
LIG_SH3_3	461	467	PF00018	0.578
LIG_SH3_3	470	476	PF00018	0.517
LIG_SUMO_SIM_anti_2	159	167	PF11976	0.309
LIG_SUMO_SIM_par_1	159	167	PF11976	0.317
LIG_SxIP_EBH_1	476	488	PF03271	0.412
LIG_TRAF2_1	194	197	PF00917	0.267
LIG_TRAF2_1	2	5	PF00917	0.434
LIG_TYR_ITIM	246	251	PF00017	0.183
LIG_UBA3_1	419	427	PF00899	0.368
LIG_WRC_WIRS_1	505	510	PF05994	0.524
MOD_CDK_SPxxK_3	366	373	PF00069	0.348
MOD_CK1_1	137	143	PF00069	0.463
MOD_CK1_1	166	172	PF00069	0.306
MOD_CK1_1	237	243	PF00069	0.552
MOD_CK1_1	404	410	PF00069	0.285
MOD_CK1_1	437	443	PF00069	0.509
MOD_CK1_1	533	539	PF00069	0.447
MOD_CK2_1	191	197	PF00069	0.262
MOD_CK2_1	200	206	PF00069	0.322
MOD_CK2_1	43	49	PF00069	0.537
MOD_CK2_1	543	549	PF00069	0.406
MOD_GlcNHglycan	136	139	PF01048	0.487
MOD_GlcNHglycan	140	143	PF01048	0.502
MOD_GlcNHglycan	167	171	PF01048	0.197
MOD_GlcNHglycan	241	244	PF01048	0.382
MOD_GlcNHglycan	480	483	PF01048	0.692
MOD_GlcNHglycan	51	54	PF01048	0.568
MOD_GSK3_1	134	141	PF00069	0.447
MOD_GSK3_1	145	152	PF00069	0.496
MOD_GSK3_1	19	26	PF00069	0.460
MOD_GSK3_1	220	227	PF00069	0.316
MOD_GSK3_1	283	290	PF00069	0.320
MOD_GSK3_1	30	37	PF00069	0.374
MOD_GSK3_1	398	405	PF00069	0.243
MOD_GSK3_1	533	540	PF00069	0.583
MOD_N-GLC_1	122	127	PF02516	0.339
MOD_N-GLC_1	218	223	PF02516	0.355
MOD_N-GLC_1	292	297	PF02516	0.294
MOD_N-GLC_1	318	323	PF02516	0.508
MOD_N-GLC_1	334	339	PF02516	0.375
MOD_N-GLC_1	49	54	PF02516	0.538
MOD_N-GLC_1	530	535	PF02516	0.718
MOD_NEK2_1	134	139	PF00069	0.466
MOD_NEK2_1	225	230	PF00069	0.392
MOD_NEK2_1	292	297	PF00069	0.332
MOD_NEK2_1	365	370	PF00069	0.393
MOD_NEK2_1	390	395	PF00069	0.266
MOD_NEK2_1	537	542	PF00069	0.604
MOD_PIKK_1	182	188	PF00454	0.348
MOD_PIKK_1	318	324	PF00454	0.396
MOD_PIKK_1	410	416	PF00454	0.280
MOD_PIKK_1	458	464	PF00454	0.613
MOD_PKA_2	182	188	PF00069	0.348
MOD_Plk_1	145	151	PF00069	0.275
MOD_Plk_1	218	224	PF00069	0.421
MOD_Plk_1	237	243	PF00069	0.284
MOD_Plk_1	24	30	PF00069	0.394
MOD_Plk_1	292	298	PF00069	0.444
MOD_Plk_1	326	332	PF00069	0.554
MOD_Plk_1	488	494	PF00069	0.683
MOD_Plk_4	200	206	PF00069	0.353
MOD_Plk_4	283	289	PF00069	0.256
MOD_Plk_4	421	427	PF00069	0.372
MOD_Plk_4	504	510	PF00069	0.571
MOD_Plk_4	537	543	PF00069	0.429
MOD_ProDKin_1	366	372	PF00069	0.298
MOD_ProDKin_1	426	432	PF00069	0.340
MOD_ProDKin_1	530	536	PF00069	0.422
MOD_SUMO_for_1	433	436	PF00179	0.371
MOD_SUMO_rev_2	2	8	PF00179	0.385
MOD_SUMO_rev_2	48	52	PF00179	0.631
TRG_DiLeu_BaEn_1	160	165	PF01217	0.282
TRG_DiLeu_BaLyEn_6	130	135	PF01217	0.319
TRG_ENDOCYTIC_2	201	204	PF00928	0.425
TRG_ENDOCYTIC_2	248	251	PF00928	0.300
TRG_ER_diArg_1	441	444	PF00400	0.420
TRG_ER_diArg_1	448	450	PF00400	0.476
TRG_NES_CRM1_1	62	76	PF08389	0.215
TRG_Pf-PMV_PEXEL_1	19	23	PF00026	0.380
TRG_Pf-PMV_PEXEL_1	373	378	PF00026	0.245
TRG_Pf-PMV_PEXEL_1	45	49	PF00026	0.624

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P473	Leptomonas seymouri	78%	99%
A0A0N1PC50	Leptomonas seymouri	29%	95%
A0A0S4IY52	Bodo saltans	29%	78%
A0A0S4JMB8	Bodo saltans	46%	79%
A0A1X0NHM5	Trypanosomatidae	29%	98%
A0A1X0P8D8	Trypanosomatidae	46%	96%
A0A3Q8IK20	Leishmania donovani	90%	100%
A0A3R7NHA6	Trypanosoma rangeli	44%	96%
A0A422N8V0	Trypanosoma rangeli	29%	100%
A4HC32	Leishmania braziliensis	31%	96%
A4ICY3	Leishmania infantum	90%	100%
D0A0Z9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	94%
D0A2G7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	96%
E9AIJ1	Leishmania braziliensis	24%	100%
E9ASC2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
E9AUS6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
Q4Q274	Leishmania major	89%	100%
Q4QCQ8	Leishmania major	25%	100%
Q9LDW3	Arabidopsis thaliana	26%	99%
Q9LP21	Arabidopsis thaliana	28%	100%
V5B1W9	Trypanosoma cruzi	29%	98%
V5BV90	Trypanosoma cruzi	45%	90%