Putative tubulin tyrosine ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tubulin tyrosine ligase, putative

Species:

Leishmania braziliensis

UniProt:

A4HNF6_LEIBR

TriTrypDb:

LbrM.34.4880 , LBRM2903_340059200

Length:

720

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	1
GO:0042995	cell projection	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0110165	cellular anatomical entity	1	1
GO:0120025	plasma membrane bounded cell projection	3	1

Expansion

Sequence features

A4HNF6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HNF6

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0019538	protein metabolic process	3	12
GO:0036211	protein modification process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044238	primary metabolic process	2	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:0000226	microtubule cytoskeleton organization	3	1
GO:0006996	organelle organization	4	1
GO:0007010	cytoskeleton organization	5	1
GO:0007017	microtubule-based process	2	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0018095	protein polyglutamylation	7	1
GO:0018193	peptidyl-amino acid modification	5	1
GO:0018200	peptidyl-glutamic acid modification	6	1
GO:0071840	cellular component organization or biogenesis	2	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004835	tubulin-tyrosine ligase activity	3	5
GO:0016874	ligase activity	2	11
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	5
GO:0016881	acid-amino acid ligase activity	4	5
GO:0140096	catalytic activity, acting on a protein	2	5
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0008092	cytoskeletal protein binding	3	1
GO:0015631	tubulin binding	4	1
GO:0070739	protein-glutamic acid ligase activity	3	1
GO:0070740	tubulin-glutamic acid ligase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	197	201	PF00656	0.620
CLV_NRD_NRD_1	170	172	PF00675	0.556
CLV_NRD_NRD_1	24	26	PF00675	0.492
CLV_NRD_NRD_1	303	305	PF00675	0.245
CLV_NRD_NRD_1	313	315	PF00675	0.243
CLV_NRD_NRD_1	341	343	PF00675	0.306
CLV_NRD_NRD_1	403	405	PF00675	0.257
CLV_NRD_NRD_1	594	596	PF00675	0.331
CLV_NRD_NRD_1	696	698	PF00675	0.467
CLV_NRD_NRD_1	75	77	PF00675	0.687
CLV_PCSK_KEX2_1	170	172	PF00082	0.546
CLV_PCSK_KEX2_1	277	279	PF00082	0.402
CLV_PCSK_KEX2_1	313	315	PF00082	0.307
CLV_PCSK_KEX2_1	341	343	PF00082	0.290
CLV_PCSK_KEX2_1	487	489	PF00082	0.299
CLV_PCSK_KEX2_1	594	596	PF00082	0.331
CLV_PCSK_KEX2_1	696	698	PF00082	0.467
CLV_PCSK_KEX2_1	77	79	PF00082	0.648
CLV_PCSK_PC1ET2_1	170	172	PF00082	0.554
CLV_PCSK_PC1ET2_1	277	279	PF00082	0.402
CLV_PCSK_PC1ET2_1	487	489	PF00082	0.306
CLV_PCSK_PC1ET2_1	77	79	PF00082	0.606
CLV_PCSK_SKI1_1	171	175	PF00082	0.557
CLV_PCSK_SKI1_1	277	281	PF00082	0.404
CLV_PCSK_SKI1_1	329	333	PF00082	0.350
CLV_PCSK_SKI1_1	514	518	PF00082	0.257
CLV_PCSK_SKI1_1	574	578	PF00082	0.238
CLV_PCSK_SKI1_1	637	641	PF00082	0.473
CLV_PCSK_SKI1_1	92	96	PF00082	0.689
DEG_APCC_DBOX_1	170	178	PF00400	0.408
DEG_APCC_DBOX_1	236	244	PF00400	0.399
DEG_SPOP_SBC_1	48	52	PF00917	0.470
DOC_CKS1_1	348	353	PF01111	0.531
DOC_CYCLIN_RxL_1	168	175	PF00134	0.575
DOC_CYCLIN_RxL_1	633	642	PF00134	0.496
DOC_CYCLIN_yClb5_NLxxxL_5	548	557	PF00134	0.520
DOC_MAPK_FxFP_2	188	191	PF00069	0.494
DOC_MAPK_gen_1	170	176	PF00069	0.495
DOC_MAPK_gen_1	404	410	PF00069	0.470
DOC_MAPK_gen_1	554	562	PF00069	0.531
DOC_MAPK_MEF2A_6	170	178	PF00069	0.488
DOC_MAPK_MEF2A_6	220	227	PF00069	0.389
DOC_MAPK_MEF2A_6	554	562	PF00069	0.531
DOC_MAPK_MEF2A_6	626	634	PF00069	0.327
DOC_MAPK_NFAT4_5	220	228	PF00069	0.448
DOC_PP2B_LxvP_1	44	47	PF13499	0.639
DOC_PP2B_PxIxI_1	353	359	PF00149	0.470
DOC_PP4_FxxP_1	102	105	PF00568	0.658
DOC_PP4_FxxP_1	15	18	PF00568	0.728
DOC_PP4_FxxP_1	188	191	PF00568	0.461
DOC_PP4_FxxP_1	331	334	PF00568	0.457
DOC_PP4_FxxP_1	643	646	PF00568	0.490
DOC_USP7_MATH_1	113	117	PF00917	0.699
DOC_USP7_MATH_1	361	365	PF00917	0.457
DOC_USP7_MATH_1	47	51	PF00917	0.708
DOC_USP7_MATH_1	532	536	PF00917	0.523
DOC_USP7_MATH_1	567	571	PF00917	0.490
DOC_USP7_MATH_1	575	579	PF00917	0.445
DOC_USP7_MATH_1	628	632	PF00917	0.561
DOC_USP7_UBL2_3	73	77	PF12436	0.508
DOC_WW_Pin1_4	347	352	PF00397	0.521
DOC_WW_Pin1_4	49	54	PF00397	0.653
LIG_14-3-3_CanoR_1	115	124	PF00244	0.566
LIG_14-3-3_CanoR_1	135	141	PF00244	0.603
LIG_14-3-3_CanoR_1	171	177	PF00244	0.475
LIG_14-3-3_CanoR_1	220	226	PF00244	0.584
LIG_14-3-3_CanoR_1	263	271	PF00244	0.512
LIG_14-3-3_CanoR_1	278	282	PF00244	0.336
LIG_14-3-3_CanoR_1	317	326	PF00244	0.370
LIG_14-3-3_CanoR_1	488	498	PF00244	0.562
LIG_14-3-3_CanoR_1	503	512	PF00244	0.445
LIG_14-3-3_CanoR_1	637	646	PF00244	0.557
LIG_14-3-3_CanoR_1	76	81	PF00244	0.660
LIG_Actin_RPEL_3	572	591	PF02755	0.486
LIG_Actin_WH2_2	247	265	PF00022	0.461
LIG_APCC_ABBA_1	422	427	PF00400	0.445
LIG_APCC_ABBAyCdc20_2	421	427	PF00400	0.445
LIG_BIR_II_1	1	5	PF00653	0.695
LIG_BRCT_BRCA1_1	186	190	PF00533	0.394
LIG_BRCT_BRCA1_1	223	227	PF00533	0.501
LIG_Clathr_ClatBox_1	542	546	PF01394	0.531
LIG_deltaCOP1_diTrp_1	478	486	PF00928	0.486
LIG_eIF4E_1	394	400	PF01652	0.506
LIG_FHA_1	12	18	PF00498	0.652
LIG_FHA_1	128	134	PF00498	0.573
LIG_FHA_1	190	196	PF00498	0.605
LIG_FHA_1	297	303	PF00498	0.485
LIG_FHA_1	32	38	PF00498	0.744
LIG_FHA_1	457	463	PF00498	0.470
LIG_FHA_1	50	56	PF00498	0.646
LIG_FHA_2	413	419	PF00498	0.445
LIG_FHA_2	640	646	PF00498	0.592
LIG_FHA_2	96	102	PF00498	0.650
LIG_LIR_Apic_2	101	105	PF02991	0.650
LIG_LIR_Apic_2	13	18	PF02991	0.712
LIG_LIR_Apic_2	187	191	PF02991	0.493
LIG_LIR_Apic_2	330	334	PF02991	0.446
LIG_LIR_Apic_2	641	646	PF02991	0.485
LIG_LIR_Gen_1	224	235	PF02991	0.529
LIG_LIR_Gen_1	270	279	PF02991	0.387
LIG_LIR_Gen_1	321	332	PF02991	0.437
LIG_LIR_Gen_1	515	524	PF02991	0.531
LIG_LIR_Nem_3	201	207	PF02991	0.467
LIG_LIR_Nem_3	224	230	PF02991	0.523
LIG_LIR_Nem_3	270	274	PF02991	0.366
LIG_LIR_Nem_3	321	327	PF02991	0.459
LIG_LIR_Nem_3	415	420	PF02991	0.445
LIG_LIR_Nem_3	499	504	PF02991	0.458
LIG_LIR_Nem_3	515	519	PF02991	0.550
LIG_LIR_Nem_3	685	690	PF02991	0.391
LIG_LIR_Nem_3	83	88	PF02991	0.626
LIG_LYPXL_yS_3	85	88	PF13949	0.617
LIG_MLH1_MIPbox_1	186	190	PF16413	0.412
LIG_MYND_1	53	57	PF01753	0.689
LIG_NRBOX	580	586	PF00104	0.384
LIG_Pex14_1	81	85	PF04695	0.405
LIG_Pex14_2	271	275	PF04695	0.337
LIG_Pex14_2	296	300	PF04695	0.445
LIG_RPA_C_Fungi	112	124	PF08784	0.718
LIG_SH2_CRK	290	294	PF00017	0.313
LIG_SH2_CRK	411	415	PF00017	0.313
LIG_SH2_SRC	107	110	PF00017	0.722
LIG_SH2_SRC	194	197	PF00017	0.353
LIG_SH2_STAP1	664	668	PF00017	0.475
LIG_SH2_STAT3	502	505	PF00017	0.192
LIG_SH2_STAT5	107	110	PF00017	0.392
LIG_SH2_STAT5	194	197	PF00017	0.577
LIG_SH2_STAT5	264	267	PF00017	0.404
LIG_SH2_STAT5	282	285	PF00017	0.392
LIG_SH2_STAT5	357	360	PF00017	0.383
LIG_SH2_STAT5	394	397	PF00017	0.298
LIG_SH2_STAT5	411	414	PF00017	0.298
LIG_SH2_STAT5	423	426	PF00017	0.298
LIG_SH2_STAT5	608	611	PF00017	0.624
LIG_SH2_STAT5	687	690	PF00017	0.362
LIG_SH2_STAT5	700	703	PF00017	0.350
LIG_SH2_STAT5	93	96	PF00017	0.604
LIG_SH3_1	374	380	PF00018	0.332
LIG_SH3_2	348	353	PF14604	0.418
LIG_SH3_3	173	179	PF00018	0.467
LIG_SH3_3	345	351	PF00018	0.405
LIG_SH3_3	374	380	PF00018	0.313
LIG_SH3_3	6	12	PF00018	0.629
LIG_SH3_3	91	97	PF00018	0.605
LIG_SUMO_SIM_par_1	129	134	PF11976	0.472
LIG_SUMO_SIM_par_1	657	663	PF11976	0.342
LIG_TRAF2_2	440	445	PF00917	0.418
LIG_UBA3_1	355	360	PF00899	0.332
LIG_WRC_WIRS_1	414	419	PF05994	0.332
LIG_WRC_WIRS_1	513	518	PF05994	0.418
LIG_WRC_WIRS_1	640	645	PF05994	0.487
MOD_CDK_SPxK_1	347	353	PF00069	0.418
MOD_CK1_1	116	122	PF00069	0.706
MOD_CK1_1	126	132	PF00069	0.500
MOD_CK1_1	138	144	PF00069	0.589
MOD_CK1_1	145	151	PF00069	0.655
MOD_CK1_1	184	190	PF00069	0.414
MOD_CK1_1	19	25	PF00069	0.719
MOD_CK1_1	198	204	PF00069	0.516
MOD_CK1_1	226	232	PF00069	0.563
MOD_CK1_1	463	469	PF00069	0.418
MOD_CK1_1	51	57	PF00069	0.610
MOD_CK1_1	565	571	PF00069	0.313
MOD_CK1_1	651	657	PF00069	0.439
MOD_CK2_1	226	232	PF00069	0.573
MOD_CK2_1	503	509	PF00069	0.367
MOD_CK2_1	519	525	PF00069	0.312
MOD_CK2_1	651	657	PF00069	0.438
MOD_Cter_Amidation	302	305	PF01082	0.298
MOD_Cter_Amidation	402	405	PF01082	0.313
MOD_GlcNHglycan	1	4	PF01048	0.698
MOD_GlcNHglycan	183	186	PF01048	0.464
MOD_GlcNHglycan	250	253	PF01048	0.469
MOD_GlcNHglycan	533	537	PF01048	0.360
MOD_GlcNHglycan	703	706	PF01048	0.412
MOD_GSK3_1	109	116	PF00069	0.649
MOD_GSK3_1	119	126	PF00069	0.446
MOD_GSK3_1	127	134	PF00069	0.596
MOD_GSK3_1	135	142	PF00069	0.605
MOD_GSK3_1	144	151	PF00069	0.645
MOD_GSK3_1	180	187	PF00069	0.508
MOD_GSK3_1	190	197	PF00069	0.553
MOD_GSK3_1	452	459	PF00069	0.323
MOD_GSK3_1	47	54	PF00069	0.659
MOD_GSK3_1	515	522	PF00069	0.259
MOD_GSK3_1	68	75	PF00069	0.580
MOD_GSK3_1	76	83	PF00069	0.538
MOD_N-GLC_1	221	226	PF02516	0.536
MOD_N-GLC_1	452	457	PF02516	0.298
MOD_NEK2_1	1	6	PF00069	0.696
MOD_NEK2_1	548	553	PF00069	0.192
MOD_NEK2_1	639	644	PF00069	0.537
MOD_NEK2_1	701	706	PF00069	0.387
MOD_PIKK_1	594	600	PF00454	0.602
MOD_PK_1	16	22	PF00069	0.632
MOD_PKA_1	277	283	PF00069	0.473
MOD_PKA_1	594	600	PF00069	0.622
MOD_PKA_1	76	82	PF00069	0.657
MOD_PKA_2	116	122	PF00069	0.676
MOD_PKA_2	198	204	PF00069	0.376
MOD_PKA_2	262	268	PF00069	0.507
MOD_PKA_2	277	283	PF00069	0.325
MOD_PKA_2	31	37	PF00069	0.486
MOD_PKA_2	594	600	PF00069	0.622
MOD_PKA_2	695	701	PF00069	0.463
MOD_PKB_1	115	123	PF00069	0.716
MOD_Plk_1	126	132	PF00069	0.690
MOD_Plk_1	221	227	PF00069	0.524
MOD_Plk_1	452	458	PF00069	0.307
MOD_Plk_1	519	525	PF00069	0.267
MOD_Plk_1	618	624	PF00069	0.540
MOD_Plk_1	648	654	PF00069	0.557
MOD_Plk_2-3	470	476	PF00069	0.332
MOD_Plk_4	172	178	PF00069	0.400
MOD_Plk_4	184	190	PF00069	0.418
MOD_Plk_4	223	229	PF00069	0.519
MOD_Plk_4	277	283	PF00069	0.491
MOD_Plk_4	327	333	PF00069	0.298
MOD_Plk_4	51	57	PF00069	0.568
MOD_Plk_4	512	518	PF00069	0.393
MOD_Plk_4	519	525	PF00069	0.343
MOD_Plk_4	583	589	PF00069	0.304
MOD_Plk_4	604	610	PF00069	0.524
MOD_Plk_4	648	654	PF00069	0.445
MOD_ProDKin_1	347	353	PF00069	0.403
MOD_ProDKin_1	49	55	PF00069	0.645
TRG_DiLeu_BaLyEn_6	351	356	PF01217	0.332
TRG_DiLeu_BaLyEn_6	395	400	PF01217	0.383
TRG_DiLeu_BaLyEn_6	553	558	PF01217	0.320
TRG_DiLeu_BaLyEn_6	635	640	PF01217	0.481
TRG_ENDOCYTIC_2	290	293	PF00928	0.313
TRG_ENDOCYTIC_2	411	414	PF00928	0.313
TRG_ENDOCYTIC_2	85	88	PF00928	0.617
TRG_ER_diArg_1	312	314	PF00400	0.414
TRG_ER_diArg_1	340	342	PF00400	0.383
TRG_ER_diArg_1	593	595	PF00400	0.418
TRG_ER_diArg_1	695	697	PF00400	0.431
TRG_NLS_MonoExtN_4	73	80	PF00514	0.615

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IAP5	Leptomonas seymouri	75%	97%
A0A0S4J7C9	Bodo saltans	51%	100%
A0A0S4KQK8	Bodo saltans	35%	100%
A0A1X0NXX8	Trypanosomatidae	30%	99%
A0A1X0P6A0	Trypanosomatidae	55%	100%
A0A3Q8III2	Leishmania donovani	86%	99%
A0A422NPL9	Trypanosoma rangeli	32%	100%
A0A422P136	Trypanosoma rangeli	55%	100%
A4IC28	Leishmania infantum	86%	99%
A4Q9E8	Mus musculus	33%	88%
C9ZI96	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	100%
C9ZYH1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	53%	100%
E9AFW7	Leishmania major	85%	100%
E9AID0	Leishmania braziliensis	28%	96%
E9AM52	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
E9B720	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	99%
Q4QCW7	Leishmania major	27%	100%
Q8N841	Homo sapiens	31%	85%
V5BVF5	Trypanosoma cruzi	30%	100%