Roadblock/LC7 domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania braziliensis

UniProt:

A4HNC4_LEIBR

TriTrypDb:

LbrM.34.4550 , LBRM2903_340056000

Length:

138

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0032045	guanyl-nucleotide exchange factor complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0071986	Ragulator complex	3	1
GO:0098796	membrane protein complex	2	1
GO:0140535	intracellular protein-containing complex	2	1

Expansion

Sequence features

A4HNC4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HNC4

Function

Biological processes
Term	Name	Level	Count
GO:0009966	regulation of signal transduction	4	12
GO:0009967	positive regulation of signal transduction	5	12
GO:0010646	regulation of cell communication	4	12
GO:0010647	positive regulation of cell communication	5	12
GO:0023051	regulation of signaling	3	12
GO:0023056	positive regulation of signaling	4	12
GO:0032006	regulation of TOR signaling	6	12
GO:0032008	positive regulation of TOR signaling	7	12
GO:0048518	positive regulation of biological process	3	12
GO:0048522	positive regulation of cellular process	4	12
GO:0048583	regulation of response to stimulus	3	12
GO:0048584	positive regulation of response to stimulus	4	12
GO:0050789	regulation of biological process	2	12
GO:0050794	regulation of cellular process	3	12
GO:0065007	biological regulation	1	12
GO:1902531	regulation of intracellular signal transduction	5	12
GO:1902533	positive regulation of intracellular signal transduction	6	12
GO:0001101	response to acid chemical	3	1
GO:0009719	response to endogenous stimulus	2	1
GO:0009987	cellular process	1	1
GO:0010033	response to organic substance	3	1
GO:0010243	response to organonitrogen compound	4	1
GO:0042221	response to chemical	2	1
GO:0043200	response to amino acid	4	1
GO:0050896	response to stimulus	1	1
GO:0051716	cellular response to stimulus	2	1
GO:0070887	cellular response to chemical stimulus	3	1
GO:0071229	cellular response to acid chemical	4	1
GO:0071230	cellular response to amino acid stimulus	5	1
GO:0071310	cellular response to organic substance	4	1
GO:0071417	cellular response to organonitrogen compound	4	1
GO:0071495	cellular response to endogenous stimulus	3	1
GO:1901698	response to nitrogen compound	3	1
GO:1901699	cellular response to nitrogen compound	4	1
GO:1901700	response to oxygen-containing compound	3	1
GO:1901701	cellular response to oxygen-containing compound	4	1

Molecular functions
Term	Name	Level	Count
GO:0005085	guanyl-nucleotide exchange factor activity	3	12
GO:0030234	enzyme regulator activity	2	12
GO:0030695	GTPase regulator activity	4	12
GO:0060090	molecular adaptor activity	1	12
GO:0060589	nucleoside-triphosphatase regulator activity	3	12
GO:0098772	molecular function regulator activity	1	12
GO:0140677	molecular function activator activity	2	12
GO:0140678	molecular function inhibitor activity	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	125	127	PF00675	0.361
CLV_PCSK_SKI1_1	110	114	PF00082	0.405
DEG_Nend_Nbox_1	1	3	PF02207	0.373
DOC_CYCLIN_yCln2_LP_2	131	137	PF00134	0.475
DOC_MAPK_gen_1	90	97	PF00069	0.330
DOC_USP7_MATH_1	101	105	PF00917	0.432
LIG_Actin_WH2_2	105	122	PF00022	0.348
LIG_BIR_III_4	26	30	PF00653	0.488
LIG_Clathr_ClatBox_1	105	109	PF01394	0.453
LIG_FHA_1	119	125	PF00498	0.372
LIG_FHA_1	75	81	PF00498	0.452
LIG_FHA_1	98	104	PF00498	0.464
LIG_LIR_Gen_1	113	124	PF02991	0.420
LIG_LIR_Nem_3	113	119	PF02991	0.441
LIG_LIR_Nem_3	44	50	PF02991	0.429
LIG_NRBOX	127	133	PF00104	0.474
LIG_NRBOX	19	25	PF00104	0.426
LIG_PCNA_yPIPBox_3	118	132	PF02747	0.418
LIG_SH2_CRK	47	51	PF00017	0.393
LIG_SH2_SRC	47	50	PF00017	0.377
LIG_SUMO_SIM_par_1	99	107	PF11976	0.449
LIG_TYR_ITIM	45	50	PF00017	0.316
MOD_CK1_1	104	110	PF00069	0.461
MOD_CK2_1	108	114	PF00069	0.411
MOD_GlcNHglycan	12	15	PF01048	0.394
MOD_GlcNHglycan	26	30	PF01048	0.395
MOD_GlcNHglycan	38	41	PF01048	0.340
MOD_GSK3_1	104	111	PF00069	0.386
MOD_GSK3_1	93	100	PF00069	0.410
MOD_NEK2_1	25	30	PF00069	0.379
MOD_NEK2_1	36	41	PF00069	0.371
MOD_Plk_1	108	114	PF00069	0.411
MOD_Plk_2-3	76	82	PF00069	0.442
MOD_Plk_4	101	107	PF00069	0.450
MOD_SUMO_rev_2	86	92	PF00179	0.312
TRG_ENDOCYTIC_2	47	50	PF00928	0.434

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P966	Leptomonas seymouri	75%	100%
A0A0S4JTI2	Bodo saltans	38%	100%
A0A1X0P543	Trypanosomatidae	45%	100%
A0A3Q8IIR8	Leishmania donovani	91%	100%
A0A422P194	Trypanosoma rangeli	43%	100%
A4IBZ7	Leishmania infantum	91%	100%
C9ZYK7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	100%
E9AFT4	Leishmania major	91%	100%
E9B6Y6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
V5BCJ6	Trypanosoma cruzi	48%	80%