Lem3 cdc50 family protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

LEM3 (ligand-effect modulator 3) family / CDC50 family, putative

Species:

Leishmania braziliensis

UniProt:

A4HN07_LEIBR

TriTrypDb:

LbrM.34.3310 , LBRM2903_340041900

Length:

422

Annotations

LeishMANIAdb annotations

Homologous to animal CDC50 family phospholipid flippase (ATPase) proteins.. This family has expanded in kinetoplastids

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 27
NetGPI	no	yes: 0, no: 27

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	28
GO:0110165	cellular anatomical entity	1	28
GO:0005783	endoplasmic reticulum	5	2
GO:0005794	Golgi apparatus	5	2
GO:0005886	plasma membrane	3	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1

Expansion

Sequence features

A4HN07

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HN07

Function

Biological processes
Term	Name	Level	Count
GO:0033036	macromolecule localization	2	28
GO:0051179	localization	1	28

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	133	137	PF00656	0.260
CLV_NRD_NRD_1	128	130	PF00675	0.412
CLV_NRD_NRD_1	332	334	PF00675	0.442
CLV_PCSK_KEX2_1	128	130	PF00082	0.412
CLV_PCSK_SKI1_1	108	112	PF00082	0.433
CLV_PCSK_SKI1_1	410	414	PF00082	0.419
DEG_SCF_FBW7_1	29	36	PF00400	0.593
DOC_CKS1_1	30	35	PF01111	0.497
DOC_CYCLIN_yCln2_LP_2	110	116	PF00134	0.202
DOC_MAPK_gen_1	108	116	PF00069	0.248
DOC_PP1_RVXF_1	241	248	PF00149	0.215
DOC_PP2B_LxvP_1	110	113	PF13499	0.232
DOC_PP4_FxxP_1	260	263	PF00568	0.247
DOC_USP7_MATH_1	148	152	PF00917	0.316
DOC_USP7_MATH_1	246	250	PF00917	0.245
DOC_USP7_MATH_1	33	37	PF00917	0.501
DOC_USP7_MATH_1	370	374	PF00917	0.260
DOC_USP7_MATH_1	57	61	PF00917	0.301
DOC_USP7_UBL2_3	252	256	PF12436	0.244
DOC_WW_Pin1_4	151	156	PF00397	0.259
DOC_WW_Pin1_4	262	267	PF00397	0.343
DOC_WW_Pin1_4	284	289	PF00397	0.212
DOC_WW_Pin1_4	29	34	PF00397	0.509
DOC_WW_Pin1_4	86	91	PF00397	0.333
LIG_14-3-3_CanoR_1	311	321	PF00244	0.273
LIG_14-3-3_CanoR_1	325	330	PF00244	0.242
LIG_14-3-3_CanoR_1	378	382	PF00244	0.328
LIG_AP2alpha_1	195	199	PF02296	0.234
LIG_CtBP_PxDLS_1	49	53	PF00389	0.413
LIG_deltaCOP1_diTrp_1	13	19	PF00928	0.417
LIG_eIF4E_1	184	190	PF01652	0.214
LIG_eIF4E_1	407	413	PF01652	0.422
LIG_FHA_1	151	157	PF00498	0.283
LIG_FHA_1	196	202	PF00498	0.244
LIG_FHA_1	4	10	PF00498	0.641
LIG_FHA_1	94	100	PF00498	0.244
LIG_FHA_2	203	209	PF00498	0.314
LIG_FHA_2	313	319	PF00498	0.231
LIG_FHA_2	86	92	PF00498	0.286
LIG_LIR_Gen_1	13	24	PF02991	0.564
LIG_LIR_Gen_1	180	190	PF02991	0.274
LIG_LIR_Gen_1	224	234	PF02991	0.301
LIG_LIR_Gen_1	399	407	PF02991	0.346
LIG_LIR_Nem_3	13	19	PF02991	0.530
LIG_LIR_Nem_3	174	179	PF02991	0.240
LIG_LIR_Nem_3	180	185	PF02991	0.242
LIG_LIR_Nem_3	197	202	PF02991	0.229
LIG_LIR_Nem_3	224	229	PF02991	0.280
LIG_LIR_Nem_3	399	403	PF02991	0.365
LIG_MYND_1	29	33	PF01753	0.514
LIG_NRBOX	414	420	PF00104	0.431
LIG_Pex14_2	195	199	PF04695	0.234
LIG_Pex14_2	299	303	PF04695	0.250
LIG_PTB_Apo_2	193	200	PF02174	0.196
LIG_SH2_CRK	255	259	PF00017	0.250
LIG_SH2_GRB2like	124	127	PF00017	0.232
LIG_SH2_GRB2like	298	301	PF00017	0.253
LIG_SH2_GRB2like	68	71	PF00017	0.260
LIG_SH2_SRC	124	127	PF00017	0.254
LIG_SH2_SRC	184	187	PF00017	0.277
LIG_SH2_STAP1	179	183	PF00017	0.327
LIG_SH2_STAT3	68	71	PF00017	0.203
LIG_SH2_STAT5	115	118	PF00017	0.194
LIG_SH2_STAT5	184	187	PF00017	0.279
LIG_SH2_STAT5	259	262	PF00017	0.321
LIG_SH2_STAT5	298	301	PF00017	0.266
LIG_SH2_STAT5	348	351	PF00017	0.238
LIG_SH2_STAT5	386	389	PF00017	0.279
LIG_SH3_1	255	261	PF00018	0.302
LIG_SH3_2	286	291	PF14604	0.187
LIG_SH3_3	180	186	PF00018	0.274
LIG_SH3_3	255	261	PF00018	0.282
LIG_SH3_3	283	289	PF00018	0.340
LIG_SH3_3	43	49	PF00018	0.346
LIG_SUMO_SIM_par_1	48	53	PF11976	0.282
LIG_UBA3_1	412	420	PF00899	0.607
LIG_WRC_WIRS_1	173	178	PF05994	0.203
MOD_CDK_SPxxK_3	284	291	PF00069	0.263
MOD_CK1_1	14	20	PF00069	0.601
MOD_CK1_1	151	157	PF00069	0.255
MOD_CK1_1	210	216	PF00069	0.212
MOD_CK1_1	224	230	PF00069	0.251
MOD_CK1_1	287	293	PF00069	0.178
MOD_CK1_1	368	374	PF00069	0.251
MOD_CK1_1	393	399	PF00069	0.422
MOD_CK1_1	89	95	PF00069	0.210
MOD_CK2_1	202	208	PF00069	0.312
MOD_CK2_1	312	318	PF00069	0.230
MOD_CK2_1	85	91	PF00069	0.276
MOD_GlcNHglycan	144	147	PF01048	0.439
MOD_GlcNHglycan	150	153	PF01048	0.428
MOD_GlcNHglycan	208	212	PF01048	0.457
MOD_GlcNHglycan	223	226	PF01048	0.484
MOD_GlcNHglycan	270	273	PF01048	0.457
MOD_GlcNHglycan	395	398	PF01048	0.357
MOD_GlcNHglycan	59	62	PF01048	0.521
MOD_GSK3_1	128	135	PF00069	0.223
MOD_GSK3_1	150	157	PF00069	0.236
MOD_GSK3_1	287	294	PF00069	0.296
MOD_GSK3_1	29	36	PF00069	0.558
MOD_GSK3_1	85	92	PF00069	0.223
MOD_GSK3_1	93	100	PF00069	0.227
MOD_N-GLC_1	195	200	PF02516	0.425
MOD_N-GLC_1	221	226	PF02516	0.452
MOD_N-GLC_1	57	62	PF02516	0.527
MOD_N-GLC_1	78	83	PF02516	0.517
MOD_NEK2_1	130	135	PF00069	0.271
MOD_NEK2_1	172	177	PF00069	0.288
MOD_NEK2_1	195	200	PF00069	0.227
MOD_NEK2_1	50	55	PF00069	0.323
MOD_NEK2_2	62	67	PF00069	0.277
MOD_PKA_1	128	134	PF00069	0.250
MOD_PKA_2	11	17	PF00069	0.604
MOD_PKA_2	128	134	PF00069	0.242
MOD_PKA_2	227	233	PF00069	0.304
MOD_PKA_2	377	383	PF00069	0.327
MOD_Plk_1	100	106	PF00069	0.189
MOD_Plk_1	195	201	PF00069	0.228
MOD_Plk_1	363	369	PF00069	0.160
MOD_Plk_1	62	68	PF00069	0.272
MOD_Plk_4	11	17	PF00069	0.600
MOD_Plk_4	172	178	PF00069	0.319
MOD_Plk_4	33	39	PF00069	0.399
MOD_Plk_4	344	350	PF00069	0.340
MOD_Plk_4	370	376	PF00069	0.238
MOD_Plk_4	377	383	PF00069	0.235
MOD_Plk_4	396	402	PF00069	0.303
MOD_Plk_4	414	420	PF00069	0.579
MOD_Plk_4	50	56	PF00069	0.347
MOD_Plk_4	89	95	PF00069	0.134
MOD_ProDKin_1	151	157	PF00069	0.259
MOD_ProDKin_1	262	268	PF00069	0.343
MOD_ProDKin_1	284	290	PF00069	0.212
MOD_ProDKin_1	29	35	PF00069	0.508
MOD_ProDKin_1	86	92	PF00069	0.333
MOD_SUMO_rev_2	249	253	PF00179	0.201
TRG_DiLeu_BaLyEn_6	168	173	PF01217	0.134
TRG_DiLeu_BaLyEn_6	215	220	PF01217	0.203
TRG_ENDOCYTIC_2	117	120	PF00928	0.229
TRG_ENDOCYTIC_2	179	182	PF00928	0.207
TRG_ENDOCYTIC_2	336	339	PF00928	0.245
TRG_ENDOCYTIC_2	386	389	PF00928	0.305
TRG_ER_diArg_1	128	130	PF00400	0.214
TRG_Pf-PMV_PEXEL_1	337	341	PF00026	0.527

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I0U8	Leptomonas seymouri	35%	67%
A0A0N1PBG2	Leptomonas seymouri	69%	100%
A0A0N1PDQ9	Leptomonas seymouri	33%	100%
A0A0S4IMK4	Bodo saltans	43%	100%
A0A0S4J4T1	Bodo saltans	33%	100%
A0A0S4JB98	Bodo saltans	35%	100%
A0A0S4JJY0	Bodo saltans	35%	100%
A0A0S4JQR9	Bodo saltans	31%	80%
A0A1X0NHQ5	Trypanosomatidae	33%	88%
A0A1X0NV71	Trypanosomatidae	32%	100%
A0A1X0P5L3	Trypanosomatidae	50%	100%
A0A3Q8IBY5	Leishmania donovani	36%	71%
A0A3R7LIN7	Trypanosoma rangeli	34%	99%
A0A3R7MGX3	Trypanosoma rangeli	32%	100%
A0A3S7X551	Leishmania donovani	30%	100%
A0A3S7X9N4	Leishmania donovani	86%	100%
A0A422NR22	Trypanosoma rangeli	50%	100%
A4H5T1	Leishmania braziliensis	35%	69%
A4HK31	Leishmania braziliensis	30%	95%
A4HU24	Leishmania infantum	36%	71%
A4I7N1	Leishmania infantum	30%	100%
A4IBN0	Leishmania infantum	86%	100%
C9ZZ02	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
D0A9K3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	90%
D0A9R9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
E9AFG4	Leishmania major	86%	100%
E9AMV4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	71%
E9B2H4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
E9B6L9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	100%
H2L0H3	Caenorhabditis elegans	28%	100%
P25656	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	100%
P42838	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	100%
P53740	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	100%
Q17QL5	Bos taurus	31%	100%
Q1MTQ5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	100%
Q2T9P5	Bos taurus	25%	100%
Q3MIR4	Homo sapiens	27%	100%
Q4Q5N2	Leishmania major	30%	91%
Q4QHS2	Leishmania major	36%	71%
Q5F362	Gallus gallus	30%	100%
Q5R6C0	Pongo abelii	31%	100%
Q67YS6	Arabidopsis thaliana	31%	100%
Q6AY41	Rattus norvegicus	32%	100%
Q8BHG3	Mus musculus	29%	100%
Q8L8W0	Arabidopsis thaliana	31%	100%
Q8VEK0	Mus musculus	32%	100%
Q95JK4	Macaca fascicularis	24%	100%
Q96WW4	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%
Q9D4D7	Mus musculus	24%	100%
Q9LTW0	Arabidopsis thaliana	31%	100%
Q9NV96	Homo sapiens	31%	100%
Q9SA35	Arabidopsis thaliana	32%	100%
Q9SLK2	Arabidopsis thaliana	30%	100%
V5AY40	Trypanosoma cruzi	35%	100%
V5B6A6	Trypanosoma cruzi	33%	100%
V5BY23	Trypanosoma cruzi	49%	100%