EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

EF-hand domain pair, putative

Species:

Leishmania braziliensis

UniProt:

A4HMY8_LEIBR

TriTrypDb:

LbrM.34.3120 , LBRM2903_340039900

Length:

648

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000159	protein phosphatase type 2A complex	5	1
GO:0008287	protein serine/threonine phosphatase complex	4	1
GO:0032991	protein-containing complex	1	1
GO:1902494	catalytic complex	2	1
GO:1903293	phosphatase complex	3	1

Expansion

Sequence features

A4HMY8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HMY8

Function

Biological processes
Term	Name	Level	Count
GO:0006470	protein dephosphorylation	5	1
GO:0006793	phosphorus metabolic process	3	1
GO:0006796	phosphate-containing compound metabolic process	4	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009987	cellular process	1	1
GO:0016311	dephosphorylation	5	1
GO:0019538	protein metabolic process	3	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	12
GO:0005509	calcium ion binding	5	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0019208	phosphatase regulator activity	3	1
GO:0019888	protein phosphatase regulator activity	4	1
GO:0030234	enzyme regulator activity	2	1
GO:0098772	molecular function regulator activity	1	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	274	278	PF00656	0.535
CLV_C14_Caspase3-7	551	555	PF00656	0.558
CLV_NRD_NRD_1	342	344	PF00675	0.246
CLV_NRD_NRD_1	580	582	PF00675	0.606
CLV_PCSK_KEX2_1	342	344	PF00082	0.246
CLV_PCSK_KEX2_1	479	481	PF00082	0.314
CLV_PCSK_PC1ET2_1	479	481	PF00082	0.314
CLV_PCSK_SKI1_1	238	242	PF00082	0.543
CLV_PCSK_SKI1_1	292	296	PF00082	0.365
CLV_PCSK_SKI1_1	301	305	PF00082	0.292
CLV_PCSK_SKI1_1	34	38	PF00082	0.599
CLV_PCSK_SKI1_1	456	460	PF00082	0.418
CLV_PCSK_SKI1_1	608	612	PF00082	0.383
DEG_APCC_DBOX_1	33	41	PF00400	0.625
DOC_CKS1_1	149	154	PF01111	0.748
DOC_CYCLIN_RxL_1	34	44	PF00134	0.512
DOC_CYCLIN_RxL_1	545	555	PF00134	0.483
DOC_CYCLIN_RxL_1	605	616	PF00134	0.523
DOC_CYCLIN_yCln2_LP_2	201	207	PF00134	0.731
DOC_MAPK_gen_1	251	259	PF00069	0.536
DOC_MAPK_gen_1	579	587	PF00069	0.516
DOC_PP1_RVXF_1	405	411	PF00149	0.471
DOC_PP1_RVXF_1	546	553	PF00149	0.471
DOC_PP1_RVXF_1	606	613	PF00149	0.485
DOC_PP2B_LxvP_1	201	204	PF13499	0.689
DOC_PP4_FxxP_1	294	297	PF00568	0.322
DOC_USP7_MATH_1	108	112	PF00917	0.730
DOC_USP7_MATH_1	168	172	PF00917	0.622
DOC_USP7_MATH_1	207	211	PF00917	0.649
DOC_USP7_MATH_1	215	219	PF00917	0.745
DOC_USP7_MATH_1	488	492	PF00917	0.314
DOC_USP7_MATH_1	69	73	PF00917	0.728
DOC_WW_Pin1_4	113	118	PF00397	0.653
DOC_WW_Pin1_4	122	127	PF00397	0.548
DOC_WW_Pin1_4	144	149	PF00397	0.616
DOC_WW_Pin1_4	151	156	PF00397	0.614
DOC_WW_Pin1_4	173	178	PF00397	0.655
DOC_WW_Pin1_4	182	187	PF00397	0.659
DOC_WW_Pin1_4	229	234	PF00397	0.585
DOC_WW_Pin1_4	552	557	PF00397	0.542
DOC_WW_Pin1_4	592	597	PF00397	0.386
DOC_WW_Pin1_4	91	96	PF00397	0.631
LIG_14-3-3_CanoR_1	179	184	PF00244	0.655
LIG_14-3-3_CanoR_1	221	231	PF00244	0.580
LIG_14-3-3_CanoR_1	328	338	PF00244	0.250
LIG_14-3-3_CanoR_1	487	494	PF00244	0.333
LIG_AP2alpha_2	616	618	PF02296	0.449
LIG_APCC_ABBA_1	283	288	PF00400	0.506
LIG_APCC_ABBAyCdc20_2	301	307	PF00400	0.457
LIG_BIR_II_1	1	5	PF00653	0.663
LIG_BIR_III_2	88	92	PF00653	0.523
LIG_BRCT_BRCA1_1	290	294	PF00533	0.451
LIG_Clathr_ClatBox_1	459	463	PF01394	0.333
LIG_deltaCOP1_diTrp_1	631	635	PF00928	0.366
LIG_FHA_1	19	25	PF00498	0.612
LIG_FHA_1	218	224	PF00498	0.627
LIG_FHA_1	280	286	PF00498	0.448
LIG_FHA_1	317	323	PF00498	0.394
LIG_FHA_1	389	395	PF00498	0.446
LIG_FHA_1	425	431	PF00498	0.413
LIG_FHA_1	473	479	PF00498	0.385
LIG_FHA_1	60	66	PF00498	0.492
LIG_FHA_2	212	218	PF00498	0.651
LIG_FHA_2	239	245	PF00498	0.470
LIG_FHA_2	356	362	PF00498	0.470
LIG_FHA_2	452	458	PF00498	0.314
LIG_FHA_2	525	531	PF00498	0.418
LIG_FHA_2	584	590	PF00498	0.378
LIG_FHA_2	628	634	PF00498	0.387
LIG_FHA_2	81	87	PF00498	0.511
LIG_FXI_DFP_1	410	414	PF00024	0.286
LIG_GBD_Chelix_1	392	400	PF00786	0.246
LIG_HCF-1_HBM_1	440	443	PF13415	0.309
LIG_LIR_Apic_2	291	297	PF02991	0.334
LIG_LIR_Apic_2	483	488	PF02991	0.418
LIG_LIR_Gen_1	302	309	PF02991	0.228
LIG_LIR_Gen_1	329	338	PF02991	0.407
LIG_LIR_Gen_1	355	362	PF02991	0.472
LIG_LIR_Gen_1	386	397	PF02991	0.532
LIG_LIR_Gen_1	438	446	PF02991	0.298
LIG_LIR_Gen_1	498	507	PF02991	0.298
LIG_LIR_Gen_1	534	542	PF02991	0.350
LIG_LIR_Gen_1	607	617	PF02991	0.439
LIG_LIR_Gen_1	630	640	PF02991	0.368
LIG_LIR_Nem_3	302	308	PF02991	0.334
LIG_LIR_Nem_3	329	334	PF02991	0.386
LIG_LIR_Nem_3	355	359	PF02991	0.472
LIG_LIR_Nem_3	383	387	PF02991	0.457
LIG_LIR_Nem_3	435	439	PF02991	0.327
LIG_LIR_Nem_3	440	446	PF02991	0.284
LIG_LIR_Nem_3	498	502	PF02991	0.298
LIG_LIR_Nem_3	607	612	PF02991	0.410
LIG_LIR_Nem_3	630	635	PF02991	0.370
LIG_MAD2	480	488	PF02301	0.314
LIG_NRBOX	268	274	PF00104	0.531
LIG_PALB2_WD40_1	517	525	PF16756	0.298
LIG_PTAP_UEV_1	162	167	PF05743	0.641
LIG_SH2_CRK	335	339	PF00017	0.425
LIG_SH2_CRK	345	349	PF00017	0.532
LIG_SH2_CRK	485	489	PF00017	0.418
LIG_SH2_NCK_1	331	335	PF00017	0.403
LIG_SH2_STAP1	20	24	PF00017	0.576
LIG_SH2_STAP1	331	335	PF00017	0.390
LIG_SH2_STAP1	439	443	PF00017	0.309
LIG_SH2_STAP1	562	566	PF00017	0.446
LIG_SH2_STAP1	640	644	PF00017	0.372
LIG_SH2_STAT5	169	172	PF00017	0.495
LIG_SH2_STAT5	20	23	PF00017	0.543
LIG_SH2_STAT5	368	371	PF00017	0.459
LIG_SH2_STAT5	436	439	PF00017	0.308
LIG_SH2_STAT5	443	446	PF00017	0.298
LIG_SH2_STAT5	504	507	PF00017	0.298
LIG_SH3_2	97	102	PF14604	0.698
LIG_SH3_3	116	122	PF00018	0.689
LIG_SH3_3	146	152	PF00018	0.659
LIG_SH3_3	160	166	PF00018	0.519
LIG_SH3_3	201	207	PF00018	0.606
LIG_SH3_3	228	234	PF00018	0.628
LIG_SH3_3	94	100	PF00018	0.632
LIG_SUMO_SIM_par_1	38	44	PF11976	0.605
LIG_TYR_ITSM	327	334	PF00017	0.440
LIG_UBA3_1	422	428	PF00899	0.532
MOD_CDC14_SPxK_1	176	179	PF00782	0.513
MOD_CDK_SPK_2	182	187	PF00069	0.739
MOD_CDK_SPK_2	91	96	PF00069	0.636
MOD_CDK_SPxK_1	173	179	PF00069	0.507
MOD_CDK_SPxxK_3	151	158	PF00069	0.735
MOD_CDK_SPxxK_3	95	102	PF00069	0.687
MOD_CK1_1	171	177	PF00069	0.507
MOD_CK1_1	182	188	PF00069	0.675
MOD_CK1_1	472	478	PF00069	0.388
MOD_CK1_1	592	598	PF00069	0.480
MOD_CK1_1	79	85	PF00069	0.696
MOD_CK2_1	211	217	PF00069	0.695
MOD_CK2_1	22	28	PF00069	0.547
MOD_CK2_1	247	253	PF00069	0.461
MOD_CK2_1	357	363	PF00069	0.502
MOD_CK2_1	531	537	PF00069	0.298
MOD_DYRK1A_RPxSP_1	144	148	PF00069	0.645
MOD_GlcNHglycan	1	4	PF01048	0.694
MOD_GlcNHglycan	105	108	PF01048	0.722
MOD_GlcNHglycan	110	113	PF01048	0.718
MOD_GlcNHglycan	162	166	PF01048	0.607
MOD_GlcNHglycan	171	174	PF01048	0.592
MOD_GlcNHglycan	197	200	PF01048	0.603
MOD_GlcNHglycan	338	341	PF01048	0.240
MOD_GlcNHglycan	43	46	PF01048	0.546
MOD_GlcNHglycan	490	493	PF01048	0.310
MOD_GlcNHglycan	533	536	PF01048	0.312
MOD_GSK3_1	144	151	PF00069	0.647
MOD_GSK3_1	169	176	PF00069	0.642
MOD_GSK3_1	18	25	PF00069	0.513
MOD_GSK3_1	205	212	PF00069	0.625
MOD_GSK3_1	225	232	PF00069	0.628
MOD_GSK3_1	247	254	PF00069	0.493
MOD_GSK3_1	275	282	PF00069	0.493
MOD_GSK3_1	303	310	PF00069	0.302
MOD_GSK3_1	334	341	PF00069	0.480
MOD_GSK3_1	357	364	PF00069	0.427
MOD_GSK3_1	59	66	PF00069	0.565
MOD_GSK3_1	76	83	PF00069	0.586
MOD_GSK3_1	91	98	PF00069	0.644
MOD_N-GLC_1	103	108	PF02516	0.712
MOD_N-GLC_1	260	265	PF02516	0.344
MOD_N-GLC_1	275	280	PF02516	0.295
MOD_N-GLC_1	604	609	PF02516	0.414
MOD_NEK2_1	188	193	PF00069	0.656
MOD_NEK2_1	19	24	PF00069	0.396
MOD_NEK2_1	211	216	PF00069	0.630
MOD_NEK2_1	222	227	PF00069	0.498
MOD_NEK2_1	338	343	PF00069	0.439
MOD_NEK2_1	347	352	PF00069	0.513
MOD_NEK2_1	41	46	PF00069	0.578
MOD_NEK2_1	78	83	PF00069	0.687
MOD_PIKK_1	6	12	PF00454	0.540
MOD_PK_1	275	281	PF00069	0.340
MOD_PKA_1	251	257	PF00069	0.507
MOD_PKA_2	215	221	PF00069	0.671
MOD_Plk_1	188	194	PF00069	0.632
MOD_Plk_1	275	281	PF00069	0.469
MOD_Plk_1	562	568	PF00069	0.426
MOD_Plk_2-3	260	266	PF00069	0.474
MOD_Plk_2-3	355	361	PF00069	0.486
MOD_Plk_2-3	435	441	PF00069	0.406
MOD_Plk_2-3	451	457	PF00069	0.198
MOD_Plk_4	19	25	PF00069	0.451
MOD_Plk_4	279	285	PF00069	0.546
MOD_Plk_4	469	475	PF00069	0.329
MOD_ProDKin_1	113	119	PF00069	0.648
MOD_ProDKin_1	122	128	PF00069	0.546
MOD_ProDKin_1	144	150	PF00069	0.616
MOD_ProDKin_1	151	157	PF00069	0.611
MOD_ProDKin_1	173	179	PF00069	0.657
MOD_ProDKin_1	182	188	PF00069	0.658
MOD_ProDKin_1	229	235	PF00069	0.586
MOD_ProDKin_1	552	558	PF00069	0.541
MOD_ProDKin_1	592	598	PF00069	0.381
MOD_ProDKin_1	91	97	PF00069	0.627
MOD_SUMO_rev_2	420	430	PF00179	0.457
MOD_SUMO_rev_2	508	514	PF00179	0.355
TRG_DiLeu_BaEn_1	268	273	PF01217	0.525
TRG_DiLeu_BaEn_4	264	270	PF01217	0.537
TRG_DiLeu_LyEn_5	268	273	PF01217	0.525
TRG_ENDOCYTIC_2	331	334	PF00928	0.399
TRG_ENDOCYTIC_2	335	338	PF00928	0.374
TRG_ENDOCYTIC_2	345	348	PF00928	0.457
TRG_ENDOCYTIC_2	439	442	PF00928	0.309
TRG_ENDOCYTIC_2	446	449	PF00928	0.298
TRG_ENDOCYTIC_2	504	507	PF00928	0.298
TRG_ENDOCYTIC_2	536	539	PF00928	0.391
TRG_ENDOCYTIC_2	640	643	PF00928	0.373
TRG_ER_diArg_1	342	344	PF00400	0.446
TRG_ER_diArg_1	623	626	PF00400	0.591
TRG_NES_CRM1_1	288	302	PF08389	0.480
TRG_Pf-PMV_PEXEL_1	342	346	PF00026	0.246

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I2Z7	Leptomonas seymouri	74%	98%
A0A0S4JHD9	Bodo saltans	40%	88%
A0A1X0P683	Trypanosomatidae	49%	98%
A0A3R7MAB5	Trypanosoma rangeli	50%	97%
A0A3S7X9I6	Leishmania donovani	84%	98%
A4IBL2	Leishmania infantum	84%	98%
C9ZZ28	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	47%	95%
E9AFE5	Leishmania major	85%	100%
E9B6K0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	98%
Q5QIT3	Arabidopsis thaliana	33%	100%
Q8VZQ4	Arabidopsis thaliana	33%	100%
Q9SLI8	Arabidopsis thaliana	33%	100%
Q9Y5P8	Homo sapiens	30%	100%
V5BY37	Trypanosoma cruzi	50%	97%