RNA_ligase domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RNA polymerase-associated protein LEO1, putative

Species:

Leishmania braziliensis

UniProt:

A4HMV2_LEIBR

TriTrypDb:

LbrM.34.2760 , LBRM2903_340036100

Length:

554

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0008023	transcription elongation factor complex	3	11
GO:0016593	Cdc73/Paf1 complex	4	11
GO:0032991	protein-containing complex	1	11
GO:0140513	nuclear protein-containing complex	2	11
GO:0005634	nucleus	5	1
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HMV2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HMV2

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006354	DNA-templated transcription elongation	6	11
GO:0006368	transcription elongation by RNA polymerase II	7	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009059	macromolecule biosynthetic process	4	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0016570	histone modification	5	11
GO:0018130	heterocycle biosynthetic process	4	11
GO:0019438	aromatic compound biosynthetic process	4	11
GO:0019538	protein metabolic process	3	11
GO:0032774	RNA biosynthetic process	5	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034654	nucleobase-containing compound biosynthetic process	4	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901362	organic cyclic compound biosynthetic process	4	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1901576	organic substance biosynthetic process	3	11
GO:0006355	regulation of DNA-templated transcription	6	1
GO:0006357	regulation of transcription by RNA polymerase II	7	1
GO:0009889	regulation of biosynthetic process	4	1
GO:0009891	positive regulation of biosynthetic process	5	1
GO:0009893	positive regulation of metabolic process	4	1
GO:0010468	regulation of gene expression	5	1
GO:0010556	regulation of macromolecule biosynthetic process	5	1
GO:0010557	positive regulation of macromolecule biosynthetic process	6	1
GO:0010604	positive regulation of macromolecule metabolic process	5	1
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	1
GO:0019222	regulation of metabolic process	3	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031325	positive regulation of cellular metabolic process	5	1
GO:0031326	regulation of cellular biosynthetic process	5	1
GO:0031328	positive regulation of cellular biosynthetic process	6	1
GO:0032784	regulation of DNA-templated transcription elongation	7	1
GO:0032786	positive regulation of DNA-templated transcription, elongation	8	1
GO:0032968	positive regulation of transcription elongation by RNA polymerase II	9	1
GO:0034243	regulation of transcription elongation by RNA polymerase II	8	1
GO:0045893	positive regulation of DNA-templated transcription	7	1
GO:0045935	positive regulation of nucleobase-containing compound metabolic process	6	1
GO:0045944	positive regulation of transcription by RNA polymerase II	8	1
GO:0048518	positive regulation of biological process	3	1
GO:0048522	positive regulation of cellular process	4	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051173	positive regulation of nitrogen compound metabolic process	5	1
GO:0051252	regulation of RNA metabolic process	5	1
GO:0051254	positive regulation of RNA metabolic process	6	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0065007	biological regulation	1	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1902680	positive regulation of RNA biosynthetic process	7	1
GO:1903506	regulation of nucleic acid-templated transcription	7	1
GO:1903508	positive regulation of nucleic acid-templated transcription	8	1
GO:2001141	regulation of RNA biosynthetic process	6	1

Molecular functions
Term	Name	Level	Count
GO:0000993	RNA polymerase II complex binding	5	1
GO:0001098	basal transcription machinery binding	3	1
GO:0001099	basal RNA polymerase II transcription machinery binding	4	1
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0019899	enzyme binding	3	1
GO:0043175	RNA polymerase core enzyme binding	5	1
GO:0045309	protein phosphorylated amino acid binding	4	1
GO:0050815	phosphoserine residue binding	5	1
GO:0051219	phosphoprotein binding	3	1
GO:0070063	RNA polymerase binding	4	1
GO:0099122	RNA polymerase II C-terminal domain binding	6	1
GO:1990269	RNA polymerase II C-terminal domain phosphoserine binding	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	431	435	PF00656	0.723
CLV_C14_Caspase3-7	438	442	PF00656	0.681
CLV_C14_Caspase3-7	472	476	PF00656	0.595
CLV_NRD_NRD_1	150	152	PF00675	0.534
CLV_NRD_NRD_1	291	293	PF00675	0.582
CLV_NRD_NRD_1	318	320	PF00675	0.612
CLV_NRD_NRD_1	342	344	PF00675	0.501
CLV_PCSK_KEX2_1	152	154	PF00082	0.636
CLV_PCSK_KEX2_1	291	293	PF00082	0.546
CLV_PCSK_KEX2_1	341	343	PF00082	0.515
CLV_PCSK_KEX2_1	399	401	PF00082	0.522
CLV_PCSK_PC1ET2_1	152	154	PF00082	0.636
CLV_PCSK_PC1ET2_1	341	343	PF00082	0.584
CLV_PCSK_PC1ET2_1	399	401	PF00082	0.663
CLV_PCSK_PC7_1	338	344	PF00082	0.605
CLV_PCSK_SKI1_1	102	106	PF00082	0.500
CLV_PCSK_SKI1_1	153	157	PF00082	0.498
CLV_PCSK_SKI1_1	192	196	PF00082	0.414
CLV_PCSK_SKI1_1	271	275	PF00082	0.477
CLV_PCSK_SKI1_1	338	342	PF00082	0.495
CLV_PCSK_SKI1_1	486	490	PF00082	0.373
CLV_PCSK_SKI1_1	71	75	PF00082	0.494
DEG_APCC_DBOX_1	485	493	PF00400	0.455
DEG_APCC_DBOX_1	542	550	PF00400	0.319
DEG_APCC_DBOX_1	70	78	PF00400	0.529
DEG_Nend_UBRbox_2	1	3	PF02207	0.513
DEG_SPOP_SBC_1	263	267	PF00917	0.193
DOC_CKS1_1	195	200	PF01111	0.404
DOC_CYCLIN_RxL_1	483	490	PF00134	0.391
DOC_CYCLIN_yCln2_LP_2	161	167	PF00134	0.526
DOC_MAPK_gen_1	102	112	PF00069	0.568
DOC_MAPK_gen_1	341	352	PF00069	0.447
DOC_MAPK_gen_1	375	384	PF00069	0.355
DOC_MAPK_gen_1	399	409	PF00069	0.571
DOC_PP1_RVXF_1	214	221	PF00149	0.271
DOC_PP2B_LxvP_1	161	164	PF13499	0.498
DOC_PP4_FxxP_1	308	311	PF00568	0.443
DOC_USP7_MATH_1	155	159	PF00917	0.512
DOC_USP7_MATH_1	498	502	PF00917	0.342
DOC_USP7_UBL2_3	341	345	PF12436	0.511
DOC_USP7_UBL2_3	464	468	PF12436	0.553
DOC_WW_Pin1_4	153	158	PF00397	0.483
DOC_WW_Pin1_4	194	199	PF00397	0.473
DOC_WW_Pin1_4	9	14	PF00397	0.668
LIG_14-3-3_CanoR_1	170	174	PF00244	0.388
LIG_14-3-3_CanoR_1	203	207	PF00244	0.360
LIG_14-3-3_CanoR_1	219	227	PF00244	0.167
LIG_14-3-3_CanoR_1	455	461	PF00244	0.481
LIG_14-3-3_CanoR_1	486	494	PF00244	0.502
LIG_AP2alpha_2	422	424	PF02296	0.694
LIG_APCC_ABBA_1	110	115	PF00400	0.547
LIG_APCC_ABBA_1	87	92	PF00400	0.598
LIG_APCC_ABBAyCdc20_2	170	176	PF00400	0.382
LIG_BRCT_BRCA1_1	189	193	PF00533	0.475
LIG_FHA_1	224	230	PF00498	0.308
LIG_FHA_1	241	247	PF00498	0.300
LIG_FHA_1	248	254	PF00498	0.352
LIG_FHA_1	263	269	PF00498	0.265
LIG_FHA_1	272	278	PF00498	0.399
LIG_FHA_1	37	43	PF00498	0.436
LIG_FHA_1	537	543	PF00498	0.457
LIG_FHA_1	68	74	PF00498	0.561
LIG_FHA_1	9	15	PF00498	0.703
LIG_FHA_2	135	141	PF00498	0.626
LIG_FHA_2	265	271	PF00498	0.336
LIG_FHA_2	321	327	PF00498	0.505
LIG_FHA_2	349	355	PF00498	0.426
LIG_FHA_2	508	514	PF00498	0.560
LIG_GBD_Chelix_1	481	489	PF00786	0.491
LIG_LIR_Apic_2	306	311	PF02991	0.577
LIG_LIR_Apic_2	314	318	PF02991	0.573
LIG_LIR_Gen_1	497	506	PF02991	0.511
LIG_LIR_Gen_1	507	515	PF02991	0.556
LIG_LIR_Nem_3	190	196	PF02991	0.536
LIG_LIR_Nem_3	475	481	PF02991	0.453
LIG_LIR_Nem_3	497	502	PF02991	0.513
LIG_NRBOX	484	490	PF00104	0.430
LIG_PCNA_yPIPBox_3	512	525	PF02747	0.342
LIG_PDZ_Class_3	549	554	PF00595	0.370
LIG_Pex14_2	48	52	PF04695	0.458
LIG_SH2_CRK	478	482	PF00017	0.369
LIG_SH2_SRC	113	116	PF00017	0.535
LIG_SH2_SRC	187	190	PF00017	0.474
LIG_SH2_STAT5	119	122	PF00017	0.538
LIG_SH2_STAT5	187	190	PF00017	0.482
LIG_SH2_STAT5	196	199	PF00017	0.311
LIG_SH2_STAT5	305	308	PF00017	0.440
LIG_SH2_STAT5	328	331	PF00017	0.319
LIG_SH3_3	162	168	PF00018	0.525
LIG_SH3_3	176	182	PF00018	0.357
LIG_SUMO_SIM_anti_2	232	239	PF11976	0.335
LIG_TRAF2_1	323	326	PF00917	0.375
LIG_UBA3_1	47	56	PF00899	0.395
MOD_CK1_1	212	218	PF00069	0.321
MOD_CK1_1	221	227	PF00069	0.229
MOD_CK1_1	239	245	PF00069	0.271
MOD_CK1_1	471	477	PF00069	0.642
MOD_CK2_1	134	140	PF00069	0.613
MOD_CK2_1	264	270	PF00069	0.417
MOD_CK2_1	320	326	PF00069	0.560
MOD_CK2_1	348	354	PF00069	0.432
MOD_CK2_1	511	517	PF00069	0.548
MOD_DYRK1A_RPxSP_1	153	157	PF00069	0.476
MOD_GlcNHglycan	121	124	PF01048	0.470
MOD_GlcNHglycan	369	372	PF01048	0.481
MOD_GlcNHglycan	418	422	PF01048	0.707
MOD_GlcNHglycan	464	467	PF01048	0.709
MOD_GSK3_1	232	239	PF00069	0.313
MOD_GSK3_1	36	43	PF00069	0.475
MOD_GSK3_1	437	444	PF00069	0.691
MOD_GSK3_1	498	505	PF00069	0.392
MOD_GSK3_1	507	514	PF00069	0.401
MOD_N-GLC_1	456	461	PF02516	0.483
MOD_N-GLC_1	511	516	PF02516	0.507
MOD_NEK2_1	139	144	PF00069	0.494
MOD_NEK2_1	202	207	PF00069	0.322
MOD_NEK2_1	67	72	PF00069	0.515
MOD_NEK2_2	223	228	PF00069	0.271
MOD_NEK2_2	412	417	PF00069	0.472
MOD_OFUCOSY	180	185	PF10250	0.562
MOD_PIKK_1	236	242	PF00454	0.404
MOD_PIKK_1	536	542	PF00454	0.473
MOD_PKA_2	169	175	PF00069	0.407
MOD_PKA_2	202	208	PF00069	0.412
MOD_PKA_2	218	224	PF00069	0.201
MOD_Plk_1	240	246	PF00069	0.395
MOD_Plk_1	306	312	PF00069	0.608
MOD_Plk_1	511	517	PF00069	0.509
MOD_Plk_2-3	279	285	PF00069	0.463
MOD_Plk_2-3	437	443	PF00069	0.561
MOD_Plk_4	155	161	PF00069	0.490
MOD_Plk_4	232	238	PF00069	0.373
MOD_Plk_4	504	510	PF00069	0.515
MOD_ProDKin_1	153	159	PF00069	0.472
MOD_ProDKin_1	194	200	PF00069	0.378
MOD_ProDKin_1	9	15	PF00069	0.668
MOD_SUMO_for_1	374	377	PF00179	0.337
MOD_SUMO_for_1	469	472	PF00179	0.637
MOD_SUMO_for_1	519	522	PF00179	0.413
MOD_SUMO_rev_2	98	106	PF00179	0.513
TRG_DiLeu_BaEn_2	188	194	PF01217	0.566
TRG_ENDOCYTIC_2	305	308	PF00928	0.495
TRG_ENDOCYTIC_2	478	481	PF00928	0.376
TRG_ER_diArg_1	291	294	PF00400	0.583
TRG_ER_diArg_1	342	344	PF00400	0.596
TRG_ER_diArg_1	400	403	PF00400	0.466
TRG_ER_KDEL_1	551	554	PF00810	0.409
TRG_NLS_MonoExtC_3	340	345	PF00514	0.591
TRG_NLS_MonoExtC_3	398	404	PF00514	0.660
TRG_NLS_MonoExtN_4	318	323	PF00514	0.610
TRG_NLS_MonoExtN_4	338	345	PF00514	0.456
TRG_NLS_MonoExtN_4	396	403	PF00514	0.655
TRG_Pf-PMV_PEXEL_1	322	326	PF00026	0.553
TRG_Pf-PMV_PEXEL_1	342	346	PF00026	0.326
TRG_Pf-PMV_PEXEL_1	405	410	PF00026	0.532
TRG_Pf-PMV_PEXEL_1	447	451	PF00026	0.410
TRG_Pf-PMV_PEXEL_1	525	529	PF00026	0.529

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMF7	Leptomonas seymouri	63%	97%
A0A1X0P5N0	Trypanosomatidae	36%	92%
A0A3Q8IMF9	Leishmania donovani	84%	100%
A0A3R7P349	Trypanosoma rangeli	38%	96%
A4IBH3	Leishmania infantum	84%	100%
C9ZZ69	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	96%
E9AFA9	Leishmania major	83%	100%
E9B6G4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	100%
V5BD17	Trypanosoma cruzi	38%	92%