rRNA adenine N(6)-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

rRNA dimethyltransferase, putative

Species:

Leishmania braziliensis

UniProt:

A4HMA9_LEIBR

TriTrypDb:

LbrM.34.0760 , LBRM2903_340012900

Length:

469

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	1
GO:0005737	cytoplasm	2	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HMA9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HMA9

Function

Biological processes
Term	Name	Level	Count
GO:0000154	rRNA modification	6	1
GO:0001510	RNA methylation	4	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006364	rRNA processing	8	1
GO:0006396	RNA processing	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0016072	rRNA metabolic process	7	1
GO:0031167	rRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000179	rRNA (adenine-N6,N6-)-dimethyltransferase activity	6	12
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008170	N-methyltransferase activity	5	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008649	rRNA methyltransferase activity	5	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016433	rRNA (adenine) methyltransferase activity	6	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140102	catalytic activity, acting on a rRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	449	453	PF00656	0.522
CLV_NRD_NRD_1	156	158	PF00675	0.488
CLV_NRD_NRD_1	214	216	PF00675	0.546
CLV_NRD_NRD_1	228	230	PF00675	0.507
CLV_PCSK_FUR_1	212	216	PF00082	0.673
CLV_PCSK_KEX2_1	156	158	PF00082	0.488
CLV_PCSK_KEX2_1	214	216	PF00082	0.544
CLV_PCSK_KEX2_1	228	230	PF00082	0.507
CLV_PCSK_SKI1_1	144	148	PF00082	0.468
CLV_PCSK_SKI1_1	287	291	PF00082	0.369
CLV_PCSK_SKI1_1	384	388	PF00082	0.262
CLV_PCSK_SKI1_1	54	58	PF00082	0.236
CLV_PCSK_SKI1_1	93	97	PF00082	0.243
CLV_Separin_Metazoa	141	145	PF03568	0.274
DEG_Nend_UBRbox_1	1	4	PF02207	0.474
DEG_ODPH_VHL_1	310	323	PF01847	0.460
DEG_SPOP_SBC_1	6	10	PF00917	0.616
DOC_ANK_TNKS_1	29	36	PF00023	0.421
DOC_ANK_TNKS_1	344	351	PF00023	0.538
DOC_CKS1_1	74	79	PF01111	0.503
DOC_CYCLIN_RxL_1	90	97	PF00134	0.436
DOC_MAPK_MEF2A_6	54	61	PF00069	0.436
DOC_MAPK_NFAT4_5	54	62	PF00069	0.436
DOC_USP7_MATH_1	201	205	PF00917	0.637
DOC_USP7_MATH_1	312	316	PF00917	0.454
DOC_USP7_MATH_1	4	8	PF00917	0.692
DOC_USP7_UBL2_3	37	41	PF12436	0.400
DOC_WW_Pin1_4	157	162	PF00397	0.693
DOC_WW_Pin1_4	239	244	PF00397	0.708
DOC_WW_Pin1_4	464	469	PF00397	0.488
DOC_WW_Pin1_4	73	78	PF00397	0.519
LIG_14-3-3_CanoR_1	30	36	PF00244	0.433
LIG_14-3-3_CanoR_1	320	324	PF00244	0.442
LIG_14-3-3_CanoR_1	332	338	PF00244	0.436
LIG_14-3-3_CanoR_1	384	389	PF00244	0.447
LIG_14-3-3_CanoR_1	426	431	PF00244	0.380
LIG_14-3-3_CanoR_1	71	77	PF00244	0.446
LIG_Actin_WH2_2	130	146	PF00022	0.470
LIG_Actin_WH2_2	370	386	PF00022	0.488
LIG_APCC_ABBA_1	112	117	PF00400	0.538
LIG_BRCT_BRCA1_1	314	318	PF00533	0.446
LIG_FHA_1	129	135	PF00498	0.558
LIG_FHA_1	271	277	PF00498	0.444
LIG_FHA_1	373	379	PF00498	0.464
LIG_FHA_1	60	66	PF00498	0.465
LIG_FHA_1	90	96	PF00498	0.440
LIG_FHA_2	334	340	PF00498	0.436
LIG_FHA_2	344	350	PF00498	0.436
LIG_FHA_2	413	419	PF00498	0.446
LIG_FHA_2	447	453	PF00498	0.639
LIG_FHA_2	73	79	PF00498	0.504
LIG_LIR_Gen_1	315	324	PF02991	0.436
LIG_LIR_Gen_1	429	437	PF02991	0.340
LIG_LIR_Nem_3	145	150	PF02991	0.377
LIG_LIR_Nem_3	315	321	PF02991	0.436
LIG_LIR_Nem_3	429	434	PF02991	0.295
LIG_PDZ_Class_1	464	469	PF00595	0.488
LIG_PTB_Apo_2	121	128	PF02174	0.547
LIG_PTB_Apo_2	241	248	PF02174	0.553
LIG_PTB_Apo_2	263	270	PF02174	0.436
LIG_REV1ctd_RIR_1	326	336	PF16727	0.538
LIG_SH2_CRK	40	44	PF00017	0.537
LIG_SH2_GRB2like	122	125	PF00017	0.520
LIG_SH2_STAP1	51	55	PF00017	0.446
LIG_SH2_STAT3	122	125	PF00017	0.487
LIG_SH2_STAT3	403	406	PF00017	0.436
LIG_SH2_STAT5	122	125	PF00017	0.451
LIG_SH2_STAT5	68	71	PF00017	0.538
LIG_SH3_3	15	21	PF00018	0.606
LIG_SH3_3	155	161	PF00018	0.607
LIG_SH3_3	23	29	PF00018	0.480
LIG_SH3_3	455	461	PF00018	0.547
LIG_SUMO_SIM_anti_2	138	145	PF11976	0.513
LIG_SUMO_SIM_anti_2	319	325	PF11976	0.436
LIG_SUMO_SIM_anti_2	418	423	PF11976	0.488
LIG_SUMO_SIM_par_1	351	356	PF11976	0.503
LIG_SUMO_SIM_par_1	415	420	PF11976	0.460
LIG_TRAF2_2	442	447	PF00917	0.589
LIG_UBA3_1	56	60	PF00899	0.436
MOD_CDK_SPxK_1	73	79	PF00069	0.503
MOD_CK1_1	223	229	PF00069	0.748
MOD_CK1_1	319	325	PF00069	0.436
MOD_CK1_1	5	11	PF00069	0.634
MOD_CK1_1	67	73	PF00069	0.465
MOD_CK1_1	94	100	PF00069	0.436
MOD_CK2_1	343	349	PF00069	0.468
MOD_CK2_1	417	423	PF00069	0.453
MOD_DYRK1A_RPxSP_1	157	161	PF00069	0.600
MOD_GlcNHglycan	144	147	PF01048	0.572
MOD_GlcNHglycan	177	180	PF01048	0.682
MOD_GlcNHglycan	203	206	PF01048	0.614
MOD_GlcNHglycan	4	7	PF01048	0.661
MOD_GSK3_1	2	9	PF00069	0.589
MOD_GSK3_1	234	241	PF00069	0.651
MOD_GSK3_1	312	319	PF00069	0.436
MOD_GSK3_1	384	391	PF00069	0.530
MOD_GSK3_1	422	429	PF00069	0.479
MOD_GSK3_1	69	76	PF00069	0.446
MOD_GSK3_1	87	94	PF00069	0.463
MOD_N-GLC_1	120	125	PF02516	0.320
MOD_NEK2_1	142	147	PF00069	0.493
MOD_NEK2_1	247	252	PF00069	0.573
MOD_NEK2_1	333	338	PF00069	0.483
MOD_NEK2_1	366	371	PF00069	0.564
MOD_NEK2_1	417	422	PF00069	0.506
MOD_NEK2_1	59	64	PF00069	0.465
MOD_NEK2_1	69	74	PF00069	0.345
MOD_NEK2_1	91	96	PF00069	0.436
MOD_PIKK_1	177	183	PF00454	0.755
MOD_PIKK_1	435	441	PF00454	0.457
MOD_PKA_2	29	35	PF00069	0.449
MOD_PKA_2	319	325	PF00069	0.442
MOD_PKA_2	70	76	PF00069	0.446
MOD_Plk_1	316	322	PF00069	0.460
MOD_Plk_1	338	344	PF00069	0.436
MOD_Plk_1	366	372	PF00069	0.520
MOD_Plk_1	417	423	PF00069	0.510
MOD_Plk_2-3	446	452	PF00069	0.521
MOD_Plk_4	319	325	PF00069	0.441
MOD_Plk_4	333	339	PF00069	0.507
MOD_Plk_4	367	373	PF00069	0.464
MOD_Plk_4	412	418	PF00069	0.448
MOD_Plk_4	426	432	PF00069	0.469
MOD_Plk_4	64	70	PF00069	0.538
MOD_ProDKin_1	157	163	PF00069	0.699
MOD_ProDKin_1	239	245	PF00069	0.703
MOD_ProDKin_1	73	79	PF00069	0.519
MOD_SUMO_for_1	259	262	PF00179	0.436
TRG_DiLeu_BaLyEn_6	206	211	PF01217	0.660
TRG_ER_diArg_1	155	157	PF00400	0.482
TRG_ER_diArg_1	211	214	PF00400	0.520
TRG_NES_CRM1_1	399	411	PF08389	0.538
TRG_Pf-PMV_PEXEL_1	209	213	PF00026	0.591
TRG_Pf-PMV_PEXEL_1	79	83	PF00026	0.252

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HXZ3	Leptomonas seymouri	78%	87%
A0A0S4IZP9	Bodo saltans	48%	88%
A0A1X0NJH8	Trypanosomatidae	54%	88%
A0A3R7NNC2	Trypanosoma rangeli	60%	98%
A0A3S7X8W9	Leishmania donovani	88%	100%
A4IAX5	Leishmania infantum	88%	100%
D0A280	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	59%	100%
E9AEQ2	Leishmania major	88%	100%
E9B5V7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
V5BN79	Trypanosoma cruzi	61%	98%