Conserved CBS domain protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

AMP-activated protein kinase, gamma regulatory subunit

Species:

Leishmania braziliensis

UniProt:

A4HMA8_LEIBR

TriTrypDb:

LbrM.34.0750 , LBRM2903_340012800

Length:

490

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005737	cytoplasm	2	1
GO:0031588	nucleotide-activated protein kinase complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	1
GO:0110165	cellular anatomical entity	1	1
GO:0140535	intracellular protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1902911	protein kinase complex	5	1
GO:1990234	transferase complex	3	1

Expansion

Sequence features

A4HMA8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HMA8

Function

Biological processes
Term	Name	Level	Count
GO:0006468	protein phosphorylation	5	1
GO:0006793	phosphorus metabolic process	3	1
GO:0006796	phosphate-containing compound metabolic process	4	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0006950	response to stress	2	1
GO:0007154	cell communication	2	1
GO:0008152	metabolic process	1	1
GO:0009267	cellular response to starvation	4	1
GO:0009605	response to external stimulus	2	1
GO:0009987	cellular process	1	1
GO:0009991	response to extracellular stimulus	3	1
GO:0016310	phosphorylation	5	1
GO:0019538	protein metabolic process	3	1
GO:0031667	response to nutrient levels	4	1
GO:0031668	cellular response to extracellular stimulus	3	1
GO:0031669	cellular response to nutrient levels	4	1
GO:0033554	cellular response to stress	3	1
GO:0036211	protein modification process	4	1
GO:0042149	cellular response to glucose starvation	5	1
GO:0042594	response to starvation	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0050790	regulation of catalytic activity	3	1
GO:0050896	response to stimulus	1	1
GO:0051716	cellular response to stimulus	2	1
GO:0065007	biological regulation	1	1
GO:0065009	regulation of molecular function	2	1
GO:0071496	cellular response to external stimulus	3	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	1
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0016208	AMP binding	4	1
GO:0017076	purine nucleotide binding	4	1
GO:0019207	kinase regulator activity	3	1
GO:0019887	protein kinase regulator activity	4	1
GO:0019899	enzyme binding	3	1
GO:0019900	kinase binding	4	1
GO:0019901	protein kinase binding	5	1
GO:0030234	enzyme regulator activity	2	1
GO:0030554	adenyl nucleotide binding	5	1
GO:0032553	ribonucleotide binding	3	1
GO:0032555	purine ribonucleotide binding	4	1
GO:0032559	adenyl ribonucleotide binding	5	1
GO:0036094	small molecule binding	2	1
GO:0043167	ion binding	2	1
GO:0043168	anion binding	3	1
GO:0043169	cation binding	3	1
GO:0097159	organic cyclic compound binding	2	1
GO:0097367	carbohydrate derivative binding	2	1
GO:0098772	molecular function regulator activity	1	1
GO:1901265	nucleoside phosphate binding	3	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	180	182	PF00675	0.355
CLV_NRD_NRD_1	364	366	PF00675	0.411
CLV_NRD_NRD_1	425	427	PF00675	0.314
CLV_NRD_NRD_1	456	458	PF00675	0.489
CLV_NRD_NRD_1	93	95	PF00675	0.581
CLV_PCSK_KEX2_1	180	182	PF00082	0.335
CLV_PCSK_KEX2_1	364	366	PF00082	0.447
CLV_PCSK_KEX2_1	455	457	PF00082	0.500
CLV_PCSK_KEX2_1	95	97	PF00082	0.564
CLV_PCSK_PC1ET2_1	95	97	PF00082	0.564
CLV_PCSK_SKI1_1	366	370	PF00082	0.490
CLV_PCSK_SKI1_1	426	430	PF00082	0.438
CLV_PCSK_SKI1_1	447	451	PF00082	0.389
CLV_PCSK_SKI1_1	456	460	PF00082	0.422
DEG_APCC_DBOX_1	406	414	PF00400	0.445
DEG_SPOP_SBC_1	101	105	PF00917	0.423
DOC_CYCLIN_RxL_1	455	465	PF00134	0.394
DOC_MAPK_gen_1	94	101	PF00069	0.536
DOC_MAPK_MEF2A_6	375	384	PF00069	0.400
DOC_MAPK_RevD_3	350	366	PF00069	0.317
DOC_PP2B_LxvP_1	148	151	PF13499	0.740
DOC_PP2B_LxvP_1	41	44	PF13499	0.416
DOC_PP2B_PxIxI_1	357	363	PF00149	0.334
DOC_PP4_FxxP_1	350	353	PF00568	0.421
DOC_USP7_MATH_1	218	222	PF00917	0.613
DOC_USP7_MATH_1	288	292	PF00917	0.664
DOC_USP7_MATH_1	327	331	PF00917	0.651
DOC_USP7_MATH_1	345	349	PF00917	0.476
DOC_USP7_MATH_1	428	432	PF00917	0.489
DOC_WW_Pin1_4	276	281	PF00397	0.728
LIG_14-3-3_CanoR_1	242	248	PF00244	0.591
LIG_14-3-3_CanoR_1	50	58	PF00244	0.421
LIG_14-3-3_CanoR_1	84	93	PF00244	0.514
LIG_APCC_ABBA_1	437	442	PF00400	0.453
LIG_BIR_II_1	1	5	PF00653	0.643
LIG_BRCT_BRCA1_1	290	294	PF00533	0.787
LIG_BRCT_BRCA1_1	346	350	PF00533	0.503
LIG_BRCT_BRCA1_1	73	77	PF00533	0.461
LIG_CaM_IQ_9	418	434	PF13499	0.551
LIG_Clathr_ClatBox_1	410	414	PF01394	0.447
LIG_Clathr_ClatBox_1	461	465	PF01394	0.381
LIG_EH1_1	176	184	PF00400	0.402
LIG_FHA_1	103	109	PF00498	0.353
LIG_FHA_1	136	142	PF00498	0.508
LIG_FHA_1	379	385	PF00498	0.410
LIG_FHA_1	386	392	PF00498	0.427
LIG_FHA_1	441	447	PF00498	0.450
LIG_FHA_1	466	472	PF00498	0.421
LIG_FHA_1	60	66	PF00498	0.440
LIG_FHA_2	101	107	PF00498	0.486
LIG_FHA_2	32	38	PF00498	0.547
LIG_FHA_2	320	326	PF00498	0.565
LIG_FHA_2	398	404	PF00498	0.349
LIG_FHA_2	443	449	PF00498	0.491
LIG_LIR_Apic_2	309	314	PF02991	0.627
LIG_LIR_Apic_2	347	353	PF02991	0.463
LIG_LIR_Gen_1	104	113	PF02991	0.367
LIG_LIR_Gen_1	130	137	PF02991	0.531
LIG_LIR_Gen_1	191	201	PF02991	0.447
LIG_LIR_Gen_1	351	362	PF02991	0.421
LIG_LIR_Gen_1	448	458	PF02991	0.407
LIG_LIR_Gen_1	479	488	PF02991	0.488
LIG_LIR_Gen_1	53	60	PF02991	0.489
LIG_LIR_Nem_3	104	110	PF02991	0.365
LIG_LIR_Nem_3	130	135	PF02991	0.521
LIG_LIR_Nem_3	191	196	PF02991	0.447
LIG_LIR_Nem_3	206	210	PF02991	0.499
LIG_LIR_Nem_3	291	297	PF02991	0.752
LIG_LIR_Nem_3	351	357	PF02991	0.402
LIG_LIR_Nem_3	435	441	PF02991	0.457
LIG_LIR_Nem_3	448	453	PF02991	0.295
LIG_LIR_Nem_3	479	485	PF02991	0.381
LIG_LIR_Nem_3	53	58	PF02991	0.416
LIG_MLH1_MIPbox_1	290	294	PF16413	0.534
LIG_PCNA_yPIPBox_3	470	481	PF02747	0.373
LIG_Pex14_2	350	354	PF04695	0.422
LIG_SH2_CRK	346	350	PF00017	0.397
LIG_SH2_CRK	55	59	PF00017	0.438
LIG_SH2_NCK_1	346	350	PF00017	0.475
LIG_SH2_PTP2	107	110	PF00017	0.315
LIG_SH2_PTP2	311	314	PF00017	0.595
LIG_SH2_PTP2	98	101	PF00017	0.434
LIG_SH2_SRC	356	359	PF00017	0.432
LIG_SH2_STAP1	346	350	PF00017	0.503
LIG_SH2_STAP1	419	423	PF00017	0.461
LIG_SH2_STAT3	419	422	PF00017	0.523
LIG_SH2_STAT5	107	110	PF00017	0.315
LIG_SH2_STAT5	113	116	PF00017	0.317
LIG_SH2_STAT5	207	210	PF00017	0.383
LIG_SH2_STAT5	293	296	PF00017	0.727
LIG_SH2_STAT5	311	314	PF00017	0.548
LIG_SH2_STAT5	316	319	PF00017	0.547
LIG_SH2_STAT5	356	359	PF00017	0.437
LIG_SH2_STAT5	482	485	PF00017	0.470
LIG_SH2_STAT5	98	101	PF00017	0.434
LIG_SH3_3	148	154	PF00018	0.687
LIG_SH3_3	3	9	PF00018	0.432
LIG_SH3_3	309	315	PF00018	0.517
LIG_SH3_3	66	72	PF00018	0.464
LIG_SUMO_SIM_anti_2	377	385	PF11976	0.422
LIG_SUMO_SIM_anti_2	400	406	PF11976	0.262
LIG_SUMO_SIM_anti_2	61	68	PF11976	0.422
LIG_SUMO_SIM_par_1	181	187	PF11976	0.361
LIG_SUMO_SIM_par_1	380	385	PF11976	0.466
LIG_SUMO_SIM_par_1	400	406	PF11976	0.150
LIG_SUMO_SIM_par_1	61	68	PF11976	0.422
LIG_TRAF2_1	34	37	PF00917	0.499
LIG_TRAF2_1	79	82	PF00917	0.418
LIG_TRFH_1	311	315	PF08558	0.600
LIG_TYR_ITIM	205	210	PF00017	0.495
LIG_TYR_ITSM	103	110	PF00017	0.367
LIG_UBA3_1	211	217	PF00899	0.538
LIG_UBA3_1	368	375	PF00899	0.480
MOD_CK1_1	248	254	PF00069	0.764
MOD_CK1_1	264	270	PF00069	0.793
MOD_CK1_1	319	325	PF00069	0.548
MOD_CK1_1	387	393	PF00069	0.227
MOD_CK1_1	431	437	PF00069	0.526
MOD_CK2_1	22	28	PF00069	0.618
MOD_CK2_1	303	309	PF00069	0.595
MOD_CK2_1	31	37	PF00069	0.449
MOD_CK2_1	319	325	PF00069	0.543
MOD_CK2_1	442	448	PF00069	0.502
MOD_GlcNHglycan	13	16	PF01048	0.507
MOD_GlcNHglycan	155	158	PF01048	0.589
MOD_GlcNHglycan	254	257	PF01048	0.654
MOD_GlcNHglycan	266	269	PF01048	0.669
MOD_GlcNHglycan	329	332	PF01048	0.656
MOD_GSK3_1	11	18	PF00069	0.546
MOD_GSK3_1	153	160	PF00069	0.581
MOD_GSK3_1	246	253	PF00069	0.754
MOD_GSK3_1	264	271	PF00069	0.794
MOD_GSK3_1	374	381	PF00069	0.426
MOD_GSK3_1	80	87	PF00069	0.538
MOD_LATS_1	17	23	PF00433	0.466
MOD_N-GLC_1	243	248	PF02516	0.737
MOD_N-GLC_1	251	256	PF02516	0.722
MOD_N-GLC_1	268	273	PF02516	0.602
MOD_N-GLC_1	283	288	PF02516	0.781
MOD_N-GLC_1	385	390	PF02516	0.227
MOD_N-GLC_1	50	55	PF02516	0.509
MOD_NEK2_1	245	250	PF00069	0.783
MOD_NEK2_1	58	63	PF00069	0.456
MOD_NEK2_2	157	162	PF00069	0.495
MOD_NEK2_2	345	350	PF00069	0.470
MOD_PIKK_1	135	141	PF00454	0.351
MOD_PIKK_1	319	325	PF00454	0.591
MOD_PIKK_1	418	424	PF00454	0.519
MOD_PIKK_1	59	65	PF00454	0.445
MOD_PKA_2	31	37	PF00069	0.572
MOD_Plk_1	163	169	PF00069	0.378
MOD_Plk_1	191	197	PF00069	0.355
MOD_Plk_1	243	249	PF00069	0.773
MOD_Plk_1	268	274	PF00069	0.706
MOD_Plk_1	50	56	PF00069	0.512
MOD_Plk_2-3	442	448	PF00069	0.502
MOD_Plk_4	22	28	PF00069	0.690
MOD_Plk_4	316	322	PF00069	0.542
MOD_Plk_4	345	351	PF00069	0.510
MOD_Plk_4	378	384	PF00069	0.447
MOD_Plk_4	397	403	PF00069	0.447
MOD_ProDKin_1	276	282	PF00069	0.727
TRG_AP2beta_CARGO_1	448	457	PF09066	0.388
TRG_DiLeu_BaEn_1	191	196	PF01217	0.447
TRG_DiLeu_BaEn_1	378	383	PF01217	0.378
TRG_DiLeu_BaEn_2	478	484	PF01217	0.363
TRG_DiLeu_BaEn_4	36	42	PF01217	0.439
TRG_DiLeu_BaLyEn_6	467	472	PF01217	0.530
TRG_ENDOCYTIC_2	107	110	PF00928	0.315
TRG_ENDOCYTIC_2	207	210	PF00928	0.495
TRG_ENDOCYTIC_2	346	349	PF00928	0.403
TRG_ENDOCYTIC_2	482	485	PF00928	0.470
TRG_ENDOCYTIC_2	55	58	PF00928	0.410
TRG_ENDOCYTIC_2	98	101	PF00928	0.434
TRG_ER_diArg_1	179	181	PF00400	0.360
TRG_ER_diArg_1	454	457	PF00400	0.413
TRG_ER_diArg_1	93	96	PF00400	0.588
TRG_NES_CRM1_1	56	68	PF08389	0.496
TRG_NLS_MonoExtN_4	93	98	PF00514	0.580
TRG_Pf-PMV_PEXEL_1	470	474	PF00026	0.498

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PCX9	Leptomonas seymouri	75%	100%
A0A1X0NKG9	Trypanosomatidae	43%	92%
A0A3Q8ILV4	Leishmania donovani	84%	100%
A0A422NI77	Trypanosoma rangeli	43%	98%
A4IAX4	Leishmania infantum	84%	100%
D0A281	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	98%
E9AEQ1	Leishmania major	84%	97%
E9B5V6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	100%
V5BSS4	Trypanosoma cruzi	49%	98%