Pyruvate kinase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Pyruvate kinase

Gene product:

pyruvate kinase

Species:

Leishmania braziliensis

UniProt:

A4HM41_LEIBR

TriTrypDb:

LbrM.34.0060 , LBRM2903_340005600

Length:

424

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 1
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 24

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 10

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 17
NetGPI	no	yes: 0, no: 17

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

A4HM41

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HM41

Function

Biological processes
Term	Name	Level	Count
GO:0005975	carbohydrate metabolic process	3	2
GO:0006082	organic acid metabolic process	3	2
GO:0006090	pyruvate metabolic process	7	2
GO:0006091	generation of precursor metabolites and energy	3	2
GO:0006096	glycolytic process	5	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006163	purine nucleotide metabolic process	5	2
GO:0006165	obsolete nucleoside diphosphate phosphorylation	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006753	nucleoside phosphate metabolic process	4	2
GO:0006757	obsolete ATP generation from ADP	4	2
GO:0006793	phosphorus metabolic process	3	2
GO:0006796	phosphate-containing compound metabolic process	4	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009117	nucleotide metabolic process	5	2
GO:0009132	nucleoside diphosphate metabolic process	5	2
GO:0009135	purine nucleoside diphosphate metabolic process	6	2
GO:0009141	nucleoside triphosphate metabolic process	5	2
GO:0009144	purine nucleoside triphosphate metabolic process	6	2
GO:0009150	purine ribonucleotide metabolic process	6	2
GO:0009179	purine ribonucleoside diphosphate metabolic process	7	2
GO:0009185	ribonucleoside diphosphate metabolic process	6	2
GO:0009199	ribonucleoside triphosphate metabolic process	6	2
GO:0009205	purine ribonucleoside triphosphate metabolic process	7	2
GO:0009259	ribonucleotide metabolic process	5	2
GO:0009987	cellular process	1	2
GO:0016052	carbohydrate catabolic process	4	2
GO:0016310	phosphorylation	5	2
GO:0019637	organophosphate metabolic process	3	2
GO:0019693	ribose phosphate metabolic process	4	2
GO:0019752	carboxylic acid metabolic process	5	2
GO:0032787	monocarboxylic acid metabolic process	6	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0043436	oxoacid metabolic process	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044281	small molecule metabolic process	2	2
GO:0046031	ADP metabolic process	7	2
GO:0046034	ATP metabolic process	7	2
GO:0046483	heterocycle metabolic process	3	2
GO:0046939	obsolete nucleotide phosphorylation	6	2
GO:0055086	nucleobase-containing small molecule metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0072521	purine-containing compound metabolic process	4	2
GO:1901135	carbohydrate derivative metabolic process	3	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1901575	organic substance catabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	18
GO:0000287	magnesium ion binding	5	18
GO:0003824	catalytic activity	1	18
GO:0004743	pyruvate kinase activity	5	18
GO:0005488	binding	1	18
GO:0005524	ATP binding	5	18
GO:0016301	kinase activity	4	18
GO:0016740	transferase activity	2	18
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	18
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	18
GO:0017076	purine nucleotide binding	4	18
GO:0030554	adenyl nucleotide binding	5	18
GO:0030955	potassium ion binding	6	18
GO:0031420	alkali metal ion binding	5	18
GO:0032553	ribonucleotide binding	3	18
GO:0032555	purine ribonucleotide binding	4	18
GO:0032559	adenyl ribonucleotide binding	5	18
GO:0035639	purine ribonucleoside triphosphate binding	4	18
GO:0036094	small molecule binding	2	18
GO:0043167	ion binding	2	18
GO:0043168	anion binding	3	18
GO:0043169	cation binding	3	18
GO:0046872	metal ion binding	4	18
GO:0097159	organic cyclic compound binding	2	18
GO:0097367	carbohydrate derivative binding	2	18
GO:1901265	nucleoside phosphate binding	3	18
GO:1901363	heterocyclic compound binding	2	18
GO:0043531	ADP binding	4	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	209	213	PF00656	0.523
CLV_NRD_NRD_1	130	132	PF00675	0.309
CLV_NRD_NRD_1	178	180	PF00675	0.383
CLV_NRD_NRD_1	4	6	PF00675	0.205
CLV_PCSK_KEX2_1	130	132	PF00082	0.349
CLV_PCSK_SKI1_1	179	183	PF00082	0.316
CLV_PCSK_SKI1_1	412	416	PF00082	0.289
CLV_PCSK_SKI1_1	59	63	PF00082	0.416
DOC_MAPK_gen_1	409	419	PF00069	0.504
DOC_MAPK_MEF2A_6	218	225	PF00069	0.502
DOC_MAPK_MEF2A_6	369	377	PF00069	0.553
DOC_USP7_MATH_1	267	271	PF00917	0.502
DOC_USP7_MATH_1	335	339	PF00917	0.502
DOC_USP7_MATH_1	340	344	PF00917	0.503
DOC_WW_Pin1_4	143	148	PF00397	0.461
DOC_WW_Pin1_4	89	94	PF00397	0.459
LIG_14-3-3_CanoR_1	380	389	PF00244	0.504
LIG_14-3-3_CanoR_1	5	15	PF00244	0.496
LIG_BIR_II_1	1	5	PF00653	0.483
LIG_BRCT_BRCA1_1	394	398	PF00533	0.598
LIG_FHA_1	250	256	PF00498	0.502
LIG_FHA_1	376	382	PF00498	0.522
LIG_FHA_1	388	394	PF00498	0.471
LIG_FHA_1	60	66	PF00498	0.540
LIG_FHA_2	124	130	PF00498	0.585
LIG_FHA_2	263	269	PF00498	0.502
LIG_FHA_2	413	419	PF00498	0.459
LIG_Integrin_RGD_1	218	220	PF01839	0.302
LIG_KLC1_Yacidic_2	78	83	PF13176	0.502
LIG_LIR_Gen_1	162	172	PF02991	0.502
LIG_LIR_Gen_1	315	322	PF02991	0.558
LIG_LIR_Gen_1	83	91	PF02991	0.573
LIG_LIR_Nem_3	162	168	PF02991	0.502
LIG_LIR_Nem_3	315	319	PF02991	0.558
LIG_LIR_Nem_3	78	84	PF02991	0.523
LIG_LRP6_Inhibitor_1	317	323	PF00058	0.405
LIG_MLH1_MIPbox_1	394	398	PF16413	0.598
LIG_PCNA_yPIPBox_3	304	317	PF02747	0.598
LIG_PTB_Apo_2	271	278	PF02174	0.537
LIG_SH2_CRK	84	88	PF00017	0.616
LIG_SH2_GRB2like	84	87	PF00017	0.616
LIG_SH2_PTP2	96	99	PF00017	0.598
LIG_SH2_SRC	96	99	PF00017	0.598
LIG_SH2_STAP1	345	349	PF00017	0.537
LIG_SH2_STAT5	125	128	PF00017	0.439
LIG_SH2_STAT5	316	319	PF00017	0.509
LIG_SH2_STAT5	81	84	PF00017	0.431
LIG_SH2_STAT5	96	99	PF00017	0.502
LIG_SH3_1	367	373	PF00018	0.502
LIG_SH3_3	130	136	PF00018	0.520
LIG_SH3_3	137	143	PF00018	0.475
LIG_SH3_3	220	226	PF00018	0.512
LIG_SH3_3	367	373	PF00018	0.502
LIG_SH3_3	87	93	PF00018	0.589
LIG_SUMO_SIM_anti_2	100	107	PF11976	0.598
LIG_SUMO_SIM_anti_2	188	193	PF11976	0.523
LIG_TRAF2_1	331	334	PF00917	0.499
LIG_TYR_ITIM	82	87	PF00017	0.512
LIG_UBA3_1	189	194	PF00899	0.523
LIG_WRC_WIRS_1	165	170	PF05994	0.502
MOD_CDK_SPK_2	143	148	PF00069	0.459
MOD_CK1_1	286	292	PF00069	0.502
MOD_CK1_1	9	15	PF00069	0.510
MOD_CK2_1	203	209	PF00069	0.571
MOD_CK2_1	262	268	PF00069	0.502
MOD_CK2_1	328	334	PF00069	0.472
MOD_CK2_1	340	346	PF00069	0.465
MOD_CK2_1	412	418	PF00069	0.459
MOD_GlcNHglycan	170	173	PF01048	0.398
MOD_GlcNHglycan	285	288	PF01048	0.302
MOD_GlcNHglycan	310	313	PF01048	0.302
MOD_GlcNHglycan	338	341	PF01048	0.283
MOD_GlcNHglycan	360	363	PF01048	0.326
MOD_GlcNHglycan	71	74	PF01048	0.280
MOD_GSK3_1	113	120	PF00069	0.598
MOD_GSK3_1	164	171	PF00069	0.512
MOD_GSK3_1	336	343	PF00069	0.508
MOD_GSK3_1	376	383	PF00069	0.506
MOD_GSK3_1	59	66	PF00069	0.553
MOD_GSK3_1	69	76	PF00069	0.553
MOD_N-GLC_1	387	392	PF02516	0.337
MOD_N-GLC_1	6	11	PF02516	0.296
MOD_NEK2_1	117	122	PF00069	0.598
MOD_NEK2_1	254	259	PF00069	0.511
MOD_NEK2_1	318	323	PF00069	0.430
MOD_NEK2_1	355	360	PF00069	0.507
MOD_NEK2_1	387	392	PF00069	0.508
MOD_NEK2_2	340	345	PF00069	0.537
MOD_NEK2_2	392	397	PF00069	0.598
MOD_PIKK_1	249	255	PF00454	0.502
MOD_PIKK_1	387	393	PF00454	0.564
MOD_PKA_2	262	268	PF00069	0.502
MOD_Plk_1	113	119	PF00069	0.503
MOD_Plk_4	164	170	PF00069	0.502
MOD_Plk_4	203	209	PF00069	0.550
MOD_Plk_4	340	346	PF00069	0.537
MOD_Plk_4	392	398	PF00069	0.528
MOD_ProDKin_1	143	149	PF00069	0.461
MOD_ProDKin_1	89	95	PF00069	0.459
MOD_SUMO_rev_2	343	349	PF00179	0.556
TRG_DiLeu_BaEn_3	209	215	PF01217	0.598
TRG_ENDOCYTIC_2	316	319	PF00928	0.509
TRG_ENDOCYTIC_2	84	87	PF00928	0.515
TRG_ENDOCYTIC_2	96	99	PF00928	0.502

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PBX8	Leptomonas seymouri	88%	85%
A0A0S4JHN6	Bodo saltans	66%	85%
A0A1X0P7Q1	Trypanosomatidae	77%	85%
A0A3Q8IES7	Leishmania donovani	92%	85%
A0A3S7X8M0	Leishmania donovani	91%	84%
A0A422NPT0	Trypanosoma rangeli	76%	85%
A4HM36	Leishmania braziliensis	99%	100%
A4HM39	Leishmania braziliensis	98%	100%
A4IAQ1	Leishmania infantum	92%	84%
A4IAQ4	Leishmania infantum	92%	93%
B0B7Q0	Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B)	40%	87%
B8BJ39	Oryza sativa subsp. indica	35%	80%
B8BM17	Oryza sativa subsp. indica	35%	80%
C9ZZZ0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	75%	85%
C9ZZZ1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	75%	85%
E9AEH9	Leishmania major	92%	100%
E9AEI0	Leishmania major	92%	100%
E9B5P1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	86%	85%
O05118	Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)	36%	89%
O08309	Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)	45%	90%
O44006	Eimeria tenella	46%	80%
O51323	Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)	37%	89%
O62619	Drosophila melanogaster	52%	80%
O65595	Arabidopsis thaliana	44%	85%
O94122	Agaricus bisporus	53%	80%
P00548	Gallus gallus	51%	80%
P00549	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	52%	85%
P0AD61	Escherichia coli (strain K12)	46%	90%
P0AD62	Escherichia coli O157:H7	46%	90%
P0CE21	Chlamydia trachomatis (strain D/UW-3/Cx)	40%	87%
P11974	Oryctolagus cuniculus	53%	80%
P11979	Felis catus	53%	80%
P11980	Rattus norvegicus	53%	80%
P12928	Rattus norvegicus	48%	74%
P14618	Homo sapiens	54%	80%
P21599	Escherichia coli (strain K12)	37%	88%
P22200	Solanum tuberosum	47%	83%
P22360	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	52%	81%
P30613	Homo sapiens	49%	74%
P30614	Yarrowia lipolytica (strain CLIB 122 / E 150)	52%	82%
P30615	Trypanosoma brucei brucei	75%	85%
P30616	Trypanosoma brucei brucei	75%	85%
P31865	Hypocrea jecorina	50%	79%
P32044	Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)	33%	78%
P34038	Lactobacillus delbrueckii subsp. bulgaricus	42%	72%
P43924	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	35%	89%
P46614	Candida albicans (strain SC5314 / ATCC MYA-2876)	53%	84%
P47458	Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)	39%	83%
P51181	Bacillus licheniformis	47%	72%
P51182	Sporosarcina psychrophila	45%	72%
P52480	Mus musculus	52%	80%
P52489	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	51%	84%
P53657	Mus musculus	48%	74%
P55964	Ricinus communis	36%	100%
P57404	Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)	33%	88%
P70789	Agrobacterium vitis	40%	89%
P73534	Synechocystis sp. (strain PCC 6803 / Kazusa)	42%	72%
P77983	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	45%	90%
P78031	Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1)	40%	83%
P80885	Bacillus subtilis (strain 168)	47%	72%
P94939	Mycobacterium intracellulare	37%	90%
P9WKE4	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	38%	90%
P9WKE5	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	38%	90%
Q02499	Geobacillus stearothermophilus	45%	72%
Q07637	Lactococcus lactis subsp. lactis (strain IL1403)	40%	84%
Q10208	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	54%	83%
Q12669	Aspergillus niger	53%	81%
Q27686	Leishmania mexicana	92%	85%
Q27788	Trypanoplasma borreli	60%	85%
Q29536	Canis lupus familiaris	49%	74%
Q2FG40	Staphylococcus aureus (strain USA300)	44%	72%
Q2FXM9	Staphylococcus aureus (strain NCTC 8325 / PS 47)	44%	72%
Q2QXR8	Oryza sativa subsp. japonica	35%	80%
Q2RAK2	Oryza sativa subsp. japonica	35%	80%
Q2YTE3	Staphylococcus aureus (strain bovine RF122 / ET3-1)	44%	72%
Q40545	Nicotiana tabacum	33%	72%
Q40546	Nicotiana tabacum	37%	75%
Q42806	Glycine max	49%	83%
Q42954	Nicotiana tabacum	45%	83%
Q43117	Ricinus communis	34%	73%
Q44473	Agrobacterium vitis	37%	88%
Q46078	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)	40%	89%
Q46289	Clostridium perfringens (strain 13 / Type A)	41%	89%
Q49YC7	Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41)	44%	72%
Q4L739	Staphylococcus haemolyticus (strain JCSC1435)	43%	72%
Q54RF5	Dictyostelium discoideum	51%	84%
Q55863	Synechocystis sp. (strain PCC 6803 / Kazusa)	38%	88%
Q56301	Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C)	38%	89%
Q57572	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	34%	95%
Q5HF76	Staphylococcus aureus (strain COL)	44%	72%
Q5HNK7	Staphylococcus epidermidis (strain ATCC 35984 / RP62A)	44%	72%
Q5NVN0	Pongo abelii	53%	80%
Q6BS75	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	50%	84%
Q6FIS9	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	53%	85%
Q6FV12	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	53%	83%
Q6G8M9	Staphylococcus aureus (strain MSSA476)	44%	72%
Q6GG09	Staphylococcus aureus (strain MRSA252)	44%	72%
Q759A9	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	51%	85%
Q7A0N4	Staphylococcus aureus (strain MW2)	44%	72%
Q7A559	Staphylococcus aureus (strain N315)	44%	72%
Q7RVA8	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	49%	80%
Q875M9	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	53%	85%
Q875S4	Lachancea kluyveri (strain ATCC 58438 / CBS 3082 / BCRC 21498 / NBRC 1685 / JCM 7257 / NCYC 543 / NRRL Y-12651)	54%	85%
Q875Z9	Naumovozyma castellii (strain ATCC 76901 / BCRC 22586 / CBS 4309 / NBRC 1992 / NRRL Y-12630)	53%	85%
Q89AI8	Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)	33%	89%
Q8CS69	Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200)	44%	72%
Q8FP04	Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)	40%	89%
Q8K9M3	Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)	35%	88%
Q8SQP0	Encephalitozoon cuniculi (strain GB-M1)	35%	99%
Q8Z6K2	Salmonella typhi	46%	90%
Q8ZNW0	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	38%	88%
Q8ZYE0	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	27%	92%
Q92122	Xenopus laevis	51%	80%
Q93Z53	Arabidopsis thaliana	37%	74%
Q99TG5	Staphylococcus aureus (strain Mu50 / ATCC 700699)	44%	72%
Q9FLW9	Arabidopsis thaliana	37%	73%
Q9LIK0	Arabidopsis thaliana	35%	71%
Q9PK61	Chlamydia muridarum (strain MoPn / Nigg)	40%	88%
Q9WY51	Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)	34%	91%
Q9YEU2	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	34%	93%
Q9Z984	Chlamydia pneumoniae	38%	88%
V5C1M1	Trypanosoma cruzi	76%	85%