ThiF domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania braziliensis

UniProt:

A4HLX6_LEIBR

TriTrypDb:

LbrM.33.2930 , LBRM2903_330037200

Length:

790

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HLX6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HLX6

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	3
GO:0008152	metabolic process	1	3
GO:0019538	protein metabolic process	3	3
GO:0032446	protein modification by small protein conjugation	6	3
GO:0036211	protein modification process	4	3
GO:0043170	macromolecule metabolic process	3	3
GO:0043412	macromolecule modification	4	3
GO:0044238	primary metabolic process	2	3
GO:0045116	protein neddylation	7	3
GO:0070647	protein modification by small protein conjugation or removal	5	3
GO:0071704	organic substance metabolic process	2	3
GO:1901564	organonitrogen compound metabolic process	3	3

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	9
GO:0008641	ubiquitin-like modifier activating enzyme activity	2	9
GO:0016874	ligase activity	2	9
GO:0016877	ligase activity, forming carbon-sulfur bonds	3	9
GO:0140096	catalytic activity, acting on a protein	2	9
GO:0140657	ATP-dependent activity	1	9
GO:0019781	NEDD8 activating enzyme activity	3	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	177	181	PF00656	0.405
CLV_C14_Caspase3-7	294	298	PF00656	0.458
CLV_C14_Caspase3-7	737	741	PF00656	0.410
CLV_NRD_NRD_1	316	318	PF00675	0.409
CLV_NRD_NRD_1	358	360	PF00675	0.458
CLV_NRD_NRD_1	625	627	PF00675	0.327
CLV_NRD_NRD_1	645	647	PF00675	0.409
CLV_NRD_NRD_1	662	664	PF00675	0.482
CLV_PCSK_KEX2_1	316	318	PF00082	0.409
CLV_PCSK_KEX2_1	358	360	PF00082	0.442
CLV_PCSK_KEX2_1	625	627	PF00082	0.317
CLV_PCSK_KEX2_1	645	647	PF00082	0.409
CLV_PCSK_KEX2_1	662	664	PF00082	0.482
CLV_PCSK_SKI1_1	21	25	PF00082	0.521
CLV_PCSK_SKI1_1	251	255	PF00082	0.266
CLV_PCSK_SKI1_1	404	408	PF00082	0.376
CLV_PCSK_SKI1_1	585	589	PF00082	0.460
CLV_PCSK_SKI1_1	67	71	PF00082	0.399
CLV_PCSK_SKI1_1	692	696	PF00082	0.537
CLV_PCSK_SKI1_1	723	727	PF00082	0.385
CLV_PCSK_SKI1_1	786	790	PF00082	0.263
CLV_Separin_Metazoa	392	396	PF03568	0.330
DEG_MDM2_SWIB_1	315	322	PF02201	0.432
DOC_CKS1_1	87	92	PF01111	0.281
DOC_CYCLIN_RxL_1	56	64	PF00134	0.241
DOC_CYCLIN_RxL_1	582	593	PF00134	0.447
DOC_CYCLIN_yCln2_LP_2	418	424	PF00134	0.316
DOC_MAPK_DCC_7	225	235	PF00069	0.415
DOC_MAPK_gen_1	21	31	PF00069	0.355
DOC_MAPK_gen_1	622	630	PF00069	0.245
DOC_MAPK_MEF2A_6	225	234	PF00069	0.390
DOC_MAPK_MEF2A_6	765	772	PF00069	0.427
DOC_PP1_RVXF_1	601	608	PF00149	0.431
DOC_PP1_RVXF_1	623	630	PF00149	0.416
DOC_PP4_FxxP_1	571	574	PF00568	0.501
DOC_PP4_FxxP_1	87	90	PF00568	0.245
DOC_USP7_MATH_1	292	296	PF00917	0.434
DOC_USP7_MATH_1	348	352	PF00917	0.466
DOC_USP7_MATH_1	408	412	PF00917	0.467
DOC_USP7_MATH_1	57	61	PF00917	0.453
DOC_USP7_MATH_1	610	614	PF00917	0.325
DOC_USP7_UBL2_3	373	377	PF12436	0.484
DOC_WW_Pin1_4	117	122	PF00397	0.332
DOC_WW_Pin1_4	210	215	PF00397	0.375
DOC_WW_Pin1_4	411	416	PF00397	0.451
DOC_WW_Pin1_4	417	422	PF00397	0.405
DOC_WW_Pin1_4	467	472	PF00397	0.500
DOC_WW_Pin1_4	575	580	PF00397	0.336
DOC_WW_Pin1_4	86	91	PF00397	0.369
DOC_WW_Pin1_4	92	97	PF00397	0.355
LIG_14-3-3_CanoR_1	145	150	PF00244	0.502
LIG_14-3-3_CanoR_1	347	353	PF00244	0.481
LIG_14-3-3_CanoR_1	612	619	PF00244	0.533
LIG_14-3-3_CanoR_1	632	637	PF00244	0.451
LIG_14-3-3_CanoR_1	67	72	PF00244	0.399
LIG_14-3-3_CanoR_1	723	732	PF00244	0.281
LIG_Actin_WH2_2	379	397	PF00022	0.435
LIG_Actin_WH2_2	577	592	PF00022	0.278
LIG_BIR_II_1	1	5	PF00653	0.651
LIG_BRCT_BRCA1_1	181	185	PF00533	0.670
LIG_BRCT_BRCA1_1	335	339	PF00533	0.532
LIG_deltaCOP1_diTrp_1	597	607	PF00928	0.384
LIG_Dynein_DLC8_1	151	157	PF01221	0.470
LIG_FHA_1	10	16	PF00498	0.580
LIG_FHA_1	127	133	PF00498	0.308
LIG_FHA_1	161	167	PF00498	0.527
LIG_FHA_1	221	227	PF00498	0.481
LIG_FHA_1	336	342	PF00498	0.443
LIG_FHA_1	373	379	PF00498	0.480
LIG_FHA_1	40	46	PF00498	0.393
LIG_FHA_1	451	457	PF00498	0.433
LIG_FHA_1	49	55	PF00498	0.384
LIG_FHA_1	490	496	PF00498	0.612
LIG_FHA_1	515	521	PF00498	0.459
LIG_FHA_1	590	596	PF00498	0.323
LIG_FHA_1	629	635	PF00498	0.420
LIG_FHA_1	66	72	PF00498	0.424
LIG_FHA_1	753	759	PF00498	0.374
LIG_FHA_1	782	788	PF00498	0.424
LIG_FHA_2	153	159	PF00498	0.459
LIG_FHA_2	340	346	PF00498	0.381
LIG_FHA_2	541	547	PF00498	0.390
LIG_FHA_2	592	598	PF00498	0.257
LIG_FHA_2	613	619	PF00498	0.359
LIG_FHA_2	68	74	PF00498	0.296
LIG_FHA_2	726	732	PF00498	0.409
LIG_LIR_Apic_2	84	90	PF02991	0.186
LIG_LIR_Gen_1	631	638	PF02991	0.557
LIG_LIR_Nem_3	206	212	PF02991	0.451
LIG_LIR_Nem_3	271	276	PF02991	0.416
LIG_LIR_Nem_3	303	307	PF02991	0.356
LIG_LIR_Nem_3	343	349	PF02991	0.389
LIG_LIR_Nem_3	519	525	PF02991	0.336
LIG_LIR_Nem_3	631	636	PF02991	0.535
LIG_LYPXL_yS_3	307	310	PF13949	0.377
LIG_NRBOX	389	395	PF00104	0.442
LIG_PDZ_Class_2	785	790	PF00595	0.444
LIG_Pex14_1	124	128	PF04695	0.348
LIG_Pex14_1	629	633	PF04695	0.262
LIG_Pex14_2	315	319	PF04695	0.410
LIG_Pex14_2	636	640	PF04695	0.387
LIG_Pex14_2	650	654	PF04695	0.245
LIG_PTB_Apo_2	782	789	PF02174	0.444
LIG_SH2_CRK	346	350	PF00017	0.429
LIG_SH2_CRK	504	508	PF00017	0.404
LIG_SH2_GRB2like	783	786	PF00017	0.454
LIG_SH2_STAP1	128	132	PF00017	0.305
LIG_SH2_STAP1	491	495	PF00017	0.404
LIG_SH2_STAP1	783	787	PF00017	0.452
LIG_SH2_STAT5	128	131	PF00017	0.311
LIG_SH2_STAT5	247	250	PF00017	0.445
LIG_SH2_STAT5	304	307	PF00017	0.321
LIG_SH2_STAT5	491	494	PF00017	0.420
LIG_SH2_STAT5	511	514	PF00017	0.317
LIG_SH2_STAT5	633	636	PF00017	0.340
LIG_SH2_STAT5	783	786	PF00017	0.454
LIG_SH2_STAT5	86	89	PF00017	0.461
LIG_SH3_1	225	231	PF00018	0.433
LIG_SH3_3	156	162	PF00018	0.506
LIG_SH3_3	194	200	PF00018	0.380
LIG_SH3_3	211	217	PF00018	0.425
LIG_SH3_3	225	231	PF00018	0.445
LIG_SH3_3	253	259	PF00018	0.536
LIG_SH3_3	493	499	PF00018	0.458
LIG_SH3_3	90	96	PF00018	0.231
LIG_SUMO_SIM_anti_2	201	207	PF11976	0.330
LIG_SUMO_SIM_anti_2	543	549	PF11976	0.238
LIG_SUMO_SIM_anti_2	68	74	PF11976	0.344
LIG_SUMO_SIM_par_1	174	182	PF11976	0.399
LIG_SUMO_SIM_par_1	201	207	PF11976	0.337
LIG_SUMO_SIM_par_1	231	238	PF11976	0.404
LIG_SUMO_SIM_par_1	50	56	PF11976	0.387
LIG_SUMO_SIM_par_1	67	74	PF11976	0.181
LIG_TRAF2_1	116	119	PF00917	0.344
LIG_TRAF2_1	323	326	PF00917	0.513
LIG_TRAF2_1	362	365	PF00917	0.385
LIG_TRAF2_1	656	659	PF00917	0.397
LIG_TRAF2_1	728	731	PF00917	0.447
LIG_TRAF2_1	759	762	PF00917	0.314
LIG_TYR_ITIM	305	310	PF00017	0.301
LIG_TYR_ITIM	520	525	PF00017	0.425
LIG_UBA3_1	576	585	PF00899	0.301
LIG_WRC_WIRS_1	349	354	PF05994	0.263
LIG_WRC_WIRS_1	633	638	PF05994	0.346
MOD_CDK_SPK_2	117	122	PF00069	0.283
MOD_CDK_SPxxK_3	467	474	PF00069	0.503
MOD_CK1_1	181	187	PF00069	0.729
MOD_CK1_1	198	204	PF00069	0.490
MOD_CK1_1	213	219	PF00069	0.299
MOD_CK1_1	221	227	PF00069	0.366
MOD_CK1_1	333	339	PF00069	0.560
MOD_CK1_1	411	417	PF00069	0.446
MOD_CK1_1	467	473	PF00069	0.500
MOD_CK1_1	476	482	PF00069	0.479
MOD_CK1_1	670	676	PF00069	0.491
MOD_CK1_1	686	692	PF00069	0.395
MOD_CK1_1	711	717	PF00069	0.338
MOD_CK2_1	117	123	PF00069	0.253
MOD_CK2_1	152	158	PF00069	0.465
MOD_CK2_1	476	482	PF00069	0.577
MOD_CK2_1	540	546	PF00069	0.229
MOD_CK2_1	591	597	PF00069	0.259
MOD_CK2_1	610	616	PF00069	0.274
MOD_CK2_1	653	659	PF00069	0.337
MOD_CK2_1	725	731	PF00069	0.408
MOD_Cter_Amidation	623	626	PF01082	0.244
MOD_GlcNHglycan	1	4	PF01048	0.744
MOD_GlcNHglycan	112	115	PF01048	0.312
MOD_GlcNHglycan	163	166	PF01048	0.473
MOD_GlcNHglycan	180	184	PF01048	0.709
MOD_GlcNHglycan	237	240	PF01048	0.387
MOD_GlcNHglycan	294	297	PF01048	0.437
MOD_GlcNHglycan	478	481	PF01048	0.552
MOD_GlcNHglycan	508	511	PF01048	0.401
MOD_GlcNHglycan	607	610	PF01048	0.485
MOD_GlcNHglycan	639	643	PF01048	0.332
MOD_GlcNHglycan	669	672	PF01048	0.570
MOD_GlcNHglycan	77	80	PF01048	0.271
MOD_GSK3_1	179	186	PF00069	0.616
MOD_GSK3_1	191	198	PF00069	0.593
MOD_GSK3_1	326	333	PF00069	0.588
MOD_GSK3_1	335	342	PF00069	0.449
MOD_GSK3_1	469	476	PF00069	0.603
MOD_GSK3_1	57	64	PF00069	0.359
MOD_GSK3_1	575	582	PF00069	0.345
MOD_GSK3_1	628	635	PF00069	0.506
MOD_GSK3_1	686	693	PF00069	0.460
MOD_GSK3_1	747	754	PF00069	0.453
MOD_GSK3_1	88	95	PF00069	0.361
MOD_N-GLC_1	186	191	PF02516	0.583
MOD_NEK2_1	1	6	PF00069	0.592
MOD_NEK2_1	10	15	PF00069	0.522
MOD_NEK2_1	185	190	PF00069	0.585
MOD_NEK2_1	218	223	PF00069	0.445
MOD_NEK2_1	234	239	PF00069	0.402
MOD_NEK2_1	243	248	PF00069	0.405
MOD_NEK2_1	450	455	PF00069	0.347
MOD_NEK2_1	48	53	PF00069	0.295
MOD_NEK2_1	515	520	PF00069	0.367
MOD_NEK2_1	61	66	PF00069	0.309
MOD_NEK2_1	672	677	PF00069	0.529
MOD_NEK2_1	708	713	PF00069	0.632
MOD_NEK2_1	97	102	PF00069	0.293
MOD_PIKK_1	152	158	PF00454	0.465
MOD_PIKK_1	257	263	PF00454	0.538
MOD_PIKK_1	683	689	PF00454	0.572
MOD_PIKK_1	718	724	PF00454	0.371
MOD_PKA_2	152	158	PF00069	0.674
MOD_PKA_2	235	241	PF00069	0.396
MOD_PKA_2	473	479	PF00069	0.510
MOD_PKA_2	605	611	PF00069	0.304
MOD_PKA_2	733	739	PF00069	0.360
MOD_Plk_1	10	16	PF00069	0.458
MOD_Plk_1	708	714	PF00069	0.537
MOD_Plk_4	198	204	PF00069	0.347
MOD_Plk_4	300	306	PF00069	0.497
MOD_Plk_4	348	354	PF00069	0.469
MOD_Plk_4	516	522	PF00069	0.424
MOD_Plk_4	57	63	PF00069	0.323
MOD_Plk_4	591	597	PF00069	0.348
MOD_Plk_4	67	73	PF00069	0.282
MOD_Plk_4	711	717	PF00069	0.338
MOD_Plk_4	97	103	PF00069	0.344
MOD_ProDKin_1	117	123	PF00069	0.332
MOD_ProDKin_1	210	216	PF00069	0.369
MOD_ProDKin_1	411	417	PF00069	0.446
MOD_ProDKin_1	467	473	PF00069	0.500
MOD_ProDKin_1	575	581	PF00069	0.324
MOD_ProDKin_1	86	92	PF00069	0.369
MOD_SUMO_rev_2	324	329	PF00179	0.499
TRG_DiLeu_BaEn_1	680	685	PF01217	0.419
TRG_DiLeu_BaEn_1	762	767	PF01217	0.310
TRG_DiLeu_BaLyEn_6	165	170	PF01217	0.258
TRG_DiLeu_BaLyEn_6	214	219	PF01217	0.440
TRG_DiLeu_BaLyEn_6	583	588	PF01217	0.442
TRG_ENDOCYTIC_2	173	176	PF00928	0.539
TRG_ENDOCYTIC_2	209	212	PF00928	0.430
TRG_ENDOCYTIC_2	307	310	PF00928	0.319
TRG_ENDOCYTIC_2	346	349	PF00928	0.337
TRG_ENDOCYTIC_2	522	525	PF00928	0.332
TRG_ENDOCYTIC_2	633	636	PF00928	0.511
TRG_ER_diArg_1	315	317	PF00400	0.397
TRG_ER_diArg_1	357	359	PF00400	0.391
TRG_ER_diArg_1	619	622	PF00400	0.431
TRG_ER_diArg_1	625	627	PF00400	0.416
TRG_ER_diArg_1	768	771	PF00400	0.306
TRG_Pf-PMV_PEXEL_1	523	527	PF00026	0.351

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5J2	Leptomonas seymouri	49%	100%
A0A0S4JRQ2	Bodo saltans	22%	96%
A0A3R7MGL1	Trypanosoma rangeli	27%	100%
A0A3S7X724	Leishmania donovani	71%	99%
A4I9A5	Leishmania infantum	71%	99%
E9B4A5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	73%	100%
Q4Q3S1	Leishmania major	72%	99%
Q5ZIE6	Gallus gallus	24%	100%
V5AV29	Trypanosoma cruzi	27%	100%