Putative aminopeptidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

metallo-peptidase, Clan MF, Family M17

Species:

Leishmania braziliensis

UniProt:

A4HLW7_LEIBR

TriTrypDb:

LbrM.33.2840 , LBRM2903_330036300

Length:

527

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 8
Pissara et al.	yes	yes: 32

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 12

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 19
NetGPI	no	yes: 0, no: 19

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	20
GO:0110165	cellular anatomical entity	1	20
GO:0005654	nucleoplasm	2	1

Expansion

Sequence features

A4HLW7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HLW7

PDB structure(s): 5nth_A

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	20
GO:0006807	nitrogen compound metabolic process	2	20
GO:0008152	metabolic process	1	20
GO:0019538	protein metabolic process	3	20
GO:0043170	macromolecule metabolic process	3	20
GO:0044238	primary metabolic process	2	20
GO:0071704	organic substance metabolic process	2	20
GO:1901564	organonitrogen compound metabolic process	3	20

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	20
GO:0004177	aminopeptidase activity	5	20
GO:0005488	binding	1	20
GO:0008233	peptidase activity	3	20
GO:0008235	metalloexopeptidase activity	5	20
GO:0008237	metallopeptidase activity	4	20
GO:0008238	exopeptidase activity	4	20
GO:0016787	hydrolase activity	2	20
GO:0030145	manganese ion binding	6	20
GO:0043167	ion binding	2	20
GO:0043169	cation binding	3	20
GO:0046872	metal ion binding	4	20
GO:0046914	transition metal ion binding	5	20
GO:0070006	metalloaminopeptidase activity	6	20
GO:0140096	catalytic activity, acting on a protein	2	20

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_PCSK_KEX2_1	170	172	PF00082	0.351
CLV_PCSK_KEX2_1	431	433	PF00082	0.378
CLV_PCSK_KEX2_1	5	7	PF00082	0.615
CLV_PCSK_PC1ET2_1	170	172	PF00082	0.384
CLV_PCSK_PC1ET2_1	431	433	PF00082	0.457
CLV_PCSK_PC1ET2_1	5	7	PF00082	0.615
CLV_PCSK_SKI1_1	288	292	PF00082	0.307
CLV_PCSK_SKI1_1	380	384	PF00082	0.347
DOC_CYCLIN_yCln2_LP_2	334	340	PF00134	0.312
DOC_CYCLIN_yCln2_LP_2	443	449	PF00134	0.369
DOC_MAPK_gen_1	363	372	PF00069	0.347
DOC_MAPK_gen_1	46	56	PF00069	0.381
DOC_MAPK_MEF2A_6	363	372	PF00069	0.401
DOC_MAPK_MEF2A_6	94	103	PF00069	0.475
DOC_PP1_RVXF_1	286	292	PF00149	0.246
DOC_PP2B_LxvP_1	334	337	PF13499	0.391
DOC_PP2B_LxvP_1	501	504	PF13499	0.431
DOC_PP2B_PxIxI_1	397	403	PF00149	0.401
DOC_PP4_FxxP_1	453	456	PF00568	0.294
DOC_USP7_MATH_1	16	20	PF00917	0.531
DOC_USP7_MATH_1	174	178	PF00917	0.460
DOC_USP7_MATH_1	304	308	PF00917	0.214
DOC_USP7_MATH_1	475	479	PF00917	0.346
DOC_USP7_MATH_2	337	343	PF00917	0.345
DOC_USP7_UBL2_3	466	470	PF12436	0.405
DOC_WW_Pin1_4	281	286	PF00397	0.347
DOC_WW_Pin1_4	394	399	PF00397	0.329
LIG_14-3-3_CanoR_1	49	54	PF00244	0.484
LIG_14-3-3_CanoR_1	94	100	PF00244	0.467
LIG_Actin_WH2_2	219	237	PF00022	0.468
LIG_Actin_WH2_2	47	65	PF00022	0.286
LIG_APCC_ABBA_1	70	75	PF00400	0.290
LIG_BIR_II_1	1	5	PF00653	0.584
LIG_BIR_III_4	376	380	PF00653	0.347
LIG_CaM_NSCaTE_8	230	237	PF13499	0.356
LIG_CtBP_PxDLS_1	248	254	PF00389	0.382
LIG_FHA_1	192	198	PF00498	0.536
LIG_FHA_1	213	219	PF00498	0.327
LIG_FHA_1	298	304	PF00498	0.356
LIG_FHA_1	339	345	PF00498	0.345
LIG_FHA_1	367	373	PF00498	0.299
LIG_FHA_1	395	401	PF00498	0.312
LIG_FHA_1	50	56	PF00498	0.391
LIG_FHA_1	94	100	PF00498	0.410
LIG_FHA_2	220	226	PF00498	0.299
LIG_FHA_2	373	379	PF00498	0.347
LIG_FHA_2	504	510	PF00498	0.431
LIG_FHA_2	82	88	PF00498	0.517
LIG_LIR_Apic_2	452	456	PF02991	0.351
LIG_LIR_Gen_1	220	231	PF02991	0.356
LIG_LIR_Gen_1	457	465	PF02991	0.337
LIG_LIR_Gen_1	495	504	PF02991	0.371
LIG_LIR_Nem_3	2	7	PF02991	0.677
LIG_LIR_Nem_3	220	226	PF02991	0.356
LIG_LIR_Nem_3	253	259	PF02991	0.347
LIG_LIR_Nem_3	33	37	PF02991	0.499
LIG_LIR_Nem_3	442	448	PF02991	0.344
LIG_LIR_Nem_3	457	462	PF02991	0.347
LIG_LIR_Nem_3	495	499	PF02991	0.319
LIG_PCNA_yPIPBox_3	233	246	PF02747	0.214
LIG_PDZ_Class_3	522	527	PF00595	0.522
LIG_PTB_Apo_2	452	459	PF02174	0.422
LIG_SH2_CRK	256	260	PF00017	0.356
LIG_SH2_CRK	4	8	PF00017	0.549
LIG_SH2_CRK	459	463	PF00017	0.348
LIG_SH2_GRB2like	178	181	PF00017	0.503
LIG_SH2_GRB2like	423	426	PF00017	0.358
LIG_SH2_NCK_1	459	463	PF00017	0.214
LIG_SH2_NCK_1	74	78	PF00017	0.468
LIG_SH2_SRC	178	181	PF00017	0.503
LIG_SH2_STAP1	459	463	PF00017	0.214
LIG_SH2_STAP1	74	78	PF00017	0.394
LIG_SH2_STAT5	146	149	PF00017	0.416
LIG_SH2_STAT5	178	181	PF00017	0.481
LIG_SH2_STAT5	35	38	PF00017	0.518
LIG_SH2_STAT5	396	399	PF00017	0.358
LIG_SH2_STAT5	449	452	PF00017	0.388
LIG_SH3_3	113	119	PF00018	0.212
LIG_SH3_3	208	214	PF00018	0.347
LIG_SH3_3	445	451	PF00018	0.411
LIG_SUMO_SIM_anti_2	196	201	PF11976	0.305
LIG_SUMO_SIM_anti_2	52	59	PF11976	0.363
LIG_SUMO_SIM_par_1	186	191	PF11976	0.445
LIG_SUMO_SIM_par_1	271	276	PF11976	0.318
LIG_SUMO_SIM_par_1	52	59	PF11976	0.398
LIG_TRAF2_1	12	15	PF00917	0.578
LIG_TRAF2_1	247	250	PF00917	0.431
LIG_UBA3_1	361	365	PF00899	0.342
MOD_CDK_SPK_2	281	286	PF00069	0.405
MOD_CDK_SPxxK_3	281	288	PF00069	0.347
MOD_CK1_1	108	114	PF00069	0.372
MOD_CK1_1	154	160	PF00069	0.334
MOD_CK1_1	191	197	PF00069	0.468
MOD_CK1_1	30	36	PF00069	0.503
MOD_CK1_1	366	372	PF00069	0.347
MOD_CK1_1	468	474	PF00069	0.419
MOD_CK2_1	219	225	PF00069	0.298
MOD_CK2_1	372	378	PF00069	0.347
MOD_CK2_1	503	509	PF00069	0.377
MOD_CK2_1	8	14	PF00069	0.546
MOD_CK2_1	81	87	PF00069	0.478
MOD_GlcNHglycan	10	13	PF01048	0.577
MOD_GlcNHglycan	18	21	PF01048	0.440
MOD_GlcNHglycan	190	193	PF01048	0.542
MOD_GlcNHglycan	365	368	PF01048	0.347
MOD_GlcNHglycan	435	438	PF01048	0.362
MOD_GlcNHglycan	459	462	PF01048	0.494
MOD_GlcNHglycan	64	68	PF01048	0.342
MOD_GSK3_1	159	166	PF00069	0.427
MOD_GSK3_1	217	224	PF00069	0.318
MOD_GSK3_1	388	395	PF00069	0.340
MOD_GSK3_1	81	88	PF00069	0.494
MOD_N-GLC_1	16	21	PF02516	0.439
MOD_N-GLC_1	163	168	PF02516	0.218
MOD_N-GLC_1	30	35	PF02516	0.477
MOD_N-GLC_1	372	377	PF02516	0.307
MOD_NEK2_1	21	26	PF00069	0.357
MOD_NEK2_1	217	222	PF00069	0.347
MOD_NEK2_1	259	264	PF00069	0.334
MOD_NEK2_1	433	438	PF00069	0.282
MOD_NEK2_1	62	67	PF00069	0.331
MOD_NEK2_2	310	315	PF00069	0.418
MOD_NEK2_2	465	470	PF00069	0.446
MOD_PIKK_1	352	358	PF00454	0.361
MOD_PK_1	105	111	PF00069	0.501
MOD_PKA_2	93	99	PF00069	0.377
MOD_PKB_1	6	14	PF00069	0.566
MOD_Plk_1	151	157	PF00069	0.452
MOD_Plk_1	16	22	PF00069	0.460
MOD_Plk_1	163	169	PF00069	0.283
MOD_Plk_1	174	180	PF00069	0.494
MOD_Plk_1	30	36	PF00069	0.394
MOD_Plk_1	338	344	PF00069	0.464
MOD_Plk_1	392	398	PF00069	0.401
MOD_Plk_1	41	47	PF00069	0.540
MOD_Plk_2-3	219	225	PF00069	0.342
MOD_Plk_4	174	180	PF00069	0.459
MOD_Plk_4	30	36	PF00069	0.358
MOD_Plk_4	339	345	PF00069	0.377
MOD_Plk_4	396	402	PF00069	0.356
MOD_Plk_4	49	55	PF00069	0.403
MOD_ProDKin_1	281	287	PF00069	0.347
MOD_ProDKin_1	394	400	PF00069	0.329
MOD_SUMO_rev_2	460	468	PF00179	0.290
TRG_DiLeu_BaLyEn_6	97	102	PF01217	0.468
TRG_ENDOCYTIC_2	256	259	PF00928	0.347
TRG_ENDOCYTIC_2	4	7	PF00928	0.657
TRG_ENDOCYTIC_2	459	462	PF00928	0.256
TRG_ER_FFAT_2	452	464	PF00635	0.372
TRG_NES_CRM1_1	359	371	PF08389	0.401
TRG_NLS_MonoExtC_3	169	175	PF00514	0.312
TRG_NLS_MonoExtC_3	4	10	PF00514	0.595

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6B6	Leptomonas seymouri	32%	97%
A0A0N0P8E6	Leptomonas seymouri	79%	100%
A0A0N1PAS4	Leptomonas seymouri	43%	98%
A0A0S4IQ79	Bodo saltans	54%	100%
A0A1X0NWR5	Trypanosomatidae	49%	100%
A0A1X0P4M9	Trypanosomatidae	48%	100%
A0A3R7JY84	Trypanosoma rangeli	48%	100%
A0A3R7N130	Trypanosoma rangeli	56%	94%
A0A3S7WRP1	Leishmania donovani	44%	99%
A0A3S7X742	Leishmania donovani	86%	100%
A0K9N9	Burkholderia cenocepacia (strain HI2424)	29%	100%
A0KPF3	Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049)	31%	100%
A1SRZ1	Psychromonas ingrahamii (strain 37)	29%	100%
A3QBG2	Shewanella loihica (strain ATCC BAA-1088 / PV-4)	29%	100%
A4H6I8	Leishmania braziliensis	43%	97%
A4HCW4	Leishmania braziliensis	32%	100%
A4HUX3	Leishmania infantum	44%	99%
A4I996	Leishmania infantum	86%	100%
A4SSA7	Aeromonas salmonicida (strain A449)	31%	100%
A5GV03	Synechococcus sp. (strain RCC307)	30%	100%
A6U7J2	Sinorhizobium medicae (strain WSM419)	34%	100%
A8LI79	Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)	32%	100%
A9AHG9	Burkholderia multivorans (strain ATCC 17616 / 249)	27%	100%
A9BBV5	Prochlorococcus marinus (strain MIT 9211)	29%	100%
B0CCF6	Acaryochloris marina (strain MBIC 11017)	29%	100%
B0U1L5	Xylella fastidiosa (strain M12)	28%	100%
B1JWV2	Burkholderia cenocepacia (strain MC0-3)	29%	100%
B1XK83	Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6)	30%	100%
B2I6L3	Xylella fastidiosa (strain M23)	27%	100%
B2J3G8	Nostoc punctiforme (strain ATCC 29133 / PCC 73102)	32%	100%
B2T146	Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)	28%	100%
B2UAK8	Ralstonia pickettii (strain 12J)	29%	100%
B4F2N1	Proteus mirabilis (strain HI4320)	29%	100%
B5F9Q6	Aliivibrio fischeri (strain MJ11)	29%	100%
B5ZWY7	Rhizobium leguminosarum bv. trifolii (strain WSM2304)	33%	100%
B8CU18	Shewanella piezotolerans (strain WP3 / JCM 13877)	28%	100%
B8GTX6	Thioalkalivibrio sulfidiphilus (strain HL-EbGR7)	29%	100%
B8HTK3	Cyanothece sp. (strain PCC 7425 / ATCC 29141)	29%	100%
B9JCD8	Agrobacterium radiobacter (strain K84 / ATCC BAA-868)	35%	100%
C0QC86	Desulforapulum autotrophicum (strain ATCC 43914 / DSM 3382 / VKM B-1955 / HRM2)	29%	100%
C3M9C8	Sinorhizobium fredii (strain NBRC 101917 / NGR234)	33%	100%
C4LA51	Tolumonas auensis (strain DSM 9187 / TA4)	29%	100%
C5CCM4	Micrococcus luteus (strain ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230)	33%	100%
C9ZV91	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	79%
D0A654	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	56%	93%
D0A7H3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	47%	100%
E9ANK8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	44%	99%
E9B496	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	100%
O06865	Synechococcus elongatus (strain PCC 7942 / FACHB-805)	31%	100%
P34629	Caenorhabditis elegans	36%	100%
Q086N8	Shewanella frigidimarina (strain NCIMB 400)	27%	100%
Q0BCQ2	Burkholderia ambifaria (strain ATCC BAA-244 / AMMD)	29%	100%
Q11A96	Trichodesmium erythraeum (strain IMS101)	30%	100%
Q12QW7	Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013)	28%	100%
Q1BUE7	Burkholderia cenocepacia (strain AU 1054)	29%	100%
Q215R8	Rhodopseudomonas palustris (strain BisB18)	33%	100%
Q39DS5	Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)	29%	100%
Q3M9J6	Trichormus variabilis (strain ATCC 29413 / PCC 7937)	31%	100%
Q488M4	Colwellia psychrerythraea (strain 34H / ATCC BAA-681)	28%	100%
Q4Q3T0	Leishmania major	85%	100%
Q4QH17	Leishmania major	44%	99%
Q5E7T8	Aliivibrio fischeri (strain ATCC 700601 / ES114)	29%	100%
Q5N569	Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1)	31%	100%
Q5QY05	Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)	29%	100%
Q5R7G6	Pongo abelii	43%	100%
Q6LUW0	Photobacterium profundum (strain SS9)	29%	100%
Q6NSR8	Mus musculus	44%	100%
Q7M8W6	Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W)	32%	100%
Q7VAP4	Prochlorococcus marinus (strain SARG / CCMP1375 / SS120)	31%	100%
Q87F32	Xylella fastidiosa (strain Temecula1 / ATCC 700964)	27%	100%
Q8K9I0	Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)	28%	100%
Q8NDH3	Homo sapiens	43%	100%
Q8Z064	Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)	31%	100%
Q92QY7	Rhizobium meliloti (strain 1021)	35%	100%
Q9PH08	Xylella fastidiosa (strain 9a5c)	27%	100%
Q9Y935	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	27%	100%
V5BTR1	Trypanosoma cruzi	56%	95%
V5BWE3	Trypanosoma cruzi	50%	100%