Putative peptidase M20/M25/M40

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

peptidase M20/M25/M40, putative

Species:

Leishmania braziliensis

UniProt:

A4HLM2_LEIBR

TriTrypDb:

LbrM.33.2100 , LBRM2903_330028100

Length:

472

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 6
Silverman et al.	no	yes: 3
Pissara et al.	yes	yes: 33

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 5

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 23
NetGPI	no	yes: 0, no: 23

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HLM2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HLM2

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	3
GO:0006807	nitrogen compound metabolic process	2	3
GO:0008152	metabolic process	1	3
GO:0019538	protein metabolic process	3	3
GO:0043170	macromolecule metabolic process	3	3
GO:0044238	primary metabolic process	2	3
GO:0071704	organic substance metabolic process	2	3
GO:1901564	organonitrogen compound metabolic process	3	3

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	24
GO:0016787	hydrolase activity	2	24
GO:0005488	binding	1	1
GO:0005506	iron ion binding	6	1
GO:0016491	oxidoreductase activity	2	1
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	3	1
GO:0043167	ion binding	2	1
GO:0043169	cation binding	3	1
GO:0046872	metal ion binding	4	1
GO:0046914	transition metal ion binding	5	1
GO:0008233	peptidase activity	3	3
GO:0140096	catalytic activity, acting on a protein	2	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_PCSK_SKI1_1	210	214	PF00082	0.405
CLV_PCSK_SKI1_1	243	247	PF00082	0.367
CLV_PCSK_SKI1_1	330	334	PF00082	0.264
CLV_PCSK_SKI1_1	356	360	PF00082	0.346
CLV_PCSK_SKI1_1	453	457	PF00082	0.381
DEG_APCC_DBOX_1	242	250	PF00400	0.366
DEG_SCF_TRCP1_1	346	351	PF00400	0.219
DOC_ANK_TNKS_1	107	114	PF00023	0.453
DOC_CYCLIN_RxL_1	240	250	PF00134	0.354
DOC_CYCLIN_RxL_1	448	460	PF00134	0.429
DOC_MAPK_gen_1	181	189	PF00069	0.428
DOC_MAPK_HePTP_8	302	314	PF00069	0.326
DOC_MAPK_MEF2A_6	183	191	PF00069	0.381
DOC_MAPK_MEF2A_6	305	314	PF00069	0.326
DOC_MAPK_MEF2A_6	330	339	PF00069	0.361
DOC_MAPK_MEF2A_6	77	85	PF00069	0.440
DOC_PP1_RVXF_1	365	371	PF00149	0.391
DOC_PP2B_LxvP_1	339	342	PF13499	0.302
DOC_PP2B_LxvP_1	468	471	PF13499	0.312
DOC_PP4_FxxP_1	307	310	PF00568	0.428
DOC_USP7_MATH_1	141	145	PF00917	0.468
DOC_USP7_MATH_1	225	229	PF00917	0.429
DOC_WW_Pin1_4	32	37	PF00397	0.441
DOC_WW_Pin1_4	378	383	PF00397	0.318
DOC_WW_Pin1_4	75	80	PF00397	0.430
LIG_14-3-3_CanoR_1	330	336	PF00244	0.377
LIG_14-3-3_CanoR_1	90	96	PF00244	0.308
LIG_Actin_WH2_2	321	338	PF00022	0.405
LIG_BIR_II_1	1	5	PF00653	0.546
LIG_BRCT_BRCA1_1	167	171	PF00533	0.371
LIG_BRCT_BRCA1_1	278	282	PF00533	0.286
LIG_BRCT_BRCA1_1	34	38	PF00533	0.531
LIG_BRCT_BRCA1_1	67	71	PF00533	0.342
LIG_FHA_1	204	210	PF00498	0.370
LIG_FHA_1	211	217	PF00498	0.370
LIG_FHA_1	236	242	PF00498	0.411
LIG_FHA_1	252	258	PF00498	0.283
LIG_FHA_1	379	385	PF00498	0.300
LIG_FHA_1	42	48	PF00498	0.504
LIG_FHA_1	429	435	PF00498	0.363
LIG_FHA_1	454	460	PF00498	0.372
LIG_FHA_1	76	82	PF00498	0.420
LIG_FHA_2	219	225	PF00498	0.381
LIG_IRF3_LxIS_1	455	460	PF10401	0.226
LIG_LIR_Gen_1	133	142	PF02991	0.410
LIG_LIR_Gen_1	197	205	PF02991	0.364
LIG_LIR_Gen_1	26	36	PF02991	0.415
LIG_LIR_Gen_1	3	13	PF02991	0.448
LIG_LIR_Gen_1	445	452	PF02991	0.378
LIG_LIR_Gen_1	49	59	PF02991	0.438
LIG_LIR_Gen_1	68	79	PF02991	0.470
LIG_LIR_Nem_3	133	139	PF02991	0.380
LIG_LIR_Nem_3	197	203	PF02991	0.364
LIG_LIR_Nem_3	26	31	PF02991	0.413
LIG_LIR_Nem_3	3	9	PF02991	0.448
LIG_LIR_Nem_3	445	449	PF02991	0.378
LIG_LIR_Nem_3	49	55	PF02991	0.477
LIG_LIR_Nem_3	68	74	PF02991	0.396
LIG_LIR_Nem_3	78	83	PF02991	0.368
LIG_LRP6_Inhibitor_1	324	330	PF00058	0.448
LIG_MYND_1	105	109	PF01753	0.370
LIG_MYND_1	382	386	PF01753	0.378
LIG_PCNA_yPIPBox_3	421	434	PF02747	0.309
LIG_Pex14_2	282	286	PF04695	0.395
LIG_Pex14_2	38	42	PF04695	0.556
LIG_Pex14_2	67	71	PF04695	0.283
LIG_PTB_Apo_2	358	365	PF02174	0.345
LIG_PTB_Phospho_1	358	364	PF10480	0.345
LIG_SH2_CRK	400	404	PF00017	0.314
LIG_SH2_CRK	406	410	PF00017	0.277
LIG_SH2_PTP2	28	31	PF00017	0.431
LIG_SH2_SRC	28	31	PF00017	0.444
LIG_SH2_STAP1	200	204	PF00017	0.370
LIG_SH2_STAT3	176	179	PF00017	0.219
LIG_SH2_STAT5	28	31	PF00017	0.431
LIG_SH3_1	103	109	PF00018	0.370
LIG_SH3_3	103	109	PF00018	0.370
LIG_SH3_3	254	260	PF00018	0.410
LIG_SH3_3	369	375	PF00018	0.253
LIG_SH3_3	430	436	PF00018	0.300
LIG_SUMO_SIM_anti_2	143	149	PF11976	0.314
LIG_SUMO_SIM_par_1	157	163	PF11976	0.368
LIG_SUMO_SIM_par_1	254	261	PF11976	0.219
LIG_SUMO_SIM_par_1	455	460	PF11976	0.226
LIG_TRAF2_1	164	167	PF00917	0.362
LIG_TRAF2_2	32	37	PF00917	0.385
LIG_TRFH_1	467	471	PF08558	0.299
LIG_TYR_ITIM	198	203	PF00017	0.314
LIG_TYR_ITIM	404	409	PF00017	0.219
MOD_CDK_SPxxK_3	378	385	PF00069	0.326
MOD_CK1_1	140	146	PF00069	0.311
MOD_CK1_1	273	279	PF00069	0.420
MOD_CK1_1	417	423	PF00069	0.219
MOD_CK2_1	218	224	PF00069	0.381
MOD_GlcNHglycan	139	142	PF01048	0.318
MOD_GlcNHglycan	166	170	PF01048	0.381
MOD_GlcNHglycan	194	197	PF01048	0.373
MOD_GlcNHglycan	227	230	PF01048	0.369
MOD_GlcNHglycan	25	28	PF01048	0.462
MOD_GlcNHglycan	292	295	PF01048	0.435
MOD_GlcNHglycan	346	349	PF01048	0.289
MOD_GSK3_1	137	144	PF00069	0.500
MOD_GSK3_1	249	256	PF00069	0.392
MOD_GSK3_1	308	315	PF00069	0.498
MOD_GSK3_1	331	338	PF00069	0.377
MOD_GSK3_1	344	351	PF00069	0.353
MOD_GSK3_1	453	460	PF00069	0.395
MOD_N-GLC_1	32	37	PF02516	0.452
MOD_N-GLC_1	91	96	PF02516	0.356
MOD_NEK2_1	203	208	PF00069	0.354
MOD_NEK2_1	312	317	PF00069	0.431
MOD_NEK2_1	335	340	PF00069	0.311
MOD_NEK2_1	344	349	PF00069	0.311
MOD_NEK2_1	357	362	PF00069	0.219
MOD_NEK2_1	415	420	PF00069	0.349
MOD_NEK2_1	428	433	PF00069	0.402
MOD_NEK2_1	457	462	PF00069	0.442
MOD_PIKK_1	348	354	PF00454	0.457
MOD_Plk_1	17	23	PF00069	0.430
MOD_Plk_1	4	10	PF00069	0.431
MOD_Plk_1	91	97	PF00069	0.580
MOD_Plk_2-3	167	173	PF00069	0.326
MOD_Plk_4	143	149	PF00069	0.444
MOD_Plk_4	17	23	PF00069	0.417
MOD_Plk_4	253	259	PF00069	0.400
MOD_Plk_4	270	276	PF00069	0.198
MOD_Plk_4	91	97	PF00069	0.453
MOD_ProDKin_1	32	38	PF00069	0.446
MOD_ProDKin_1	378	384	PF00069	0.318
MOD_ProDKin_1	75	81	PF00069	0.426
MOD_SUMO_rev_2	247	254	PF00179	0.428
MOD_SUMO_rev_2	377	386	PF00179	0.256
TRG_ENDOCYTIC_2	136	139	PF00928	0.443
TRG_ENDOCYTIC_2	200	203	PF00928	0.377
TRG_ENDOCYTIC_2	28	31	PF00928	0.431
TRG_ENDOCYTIC_2	406	409	PF00928	0.392
TRG_ENDOCYTIC_2	69	72	PF00928	0.436
TRG_ENDOCYTIC_2	98	101	PF00928	0.370
TRG_NES_CRM1_1	182	194	PF08389	0.499
TRG_Pf-PMV_PEXEL_1	243	247	PF00026	0.316

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1Q9	Leptomonas seymouri	82%	100%
A0A0N1PBD8	Leptomonas seymouri	72%	100%
A0A0S4JJE7	Bodo saltans	59%	100%
A0A1X0P1Q0	Trypanosomatidae	67%	100%
A0A381MQ29	Leishmania infantum	72%	100%
A0A3Q8IFP2	Leishmania donovani	71%	83%
A0A3R7KSZ4	Trypanosoma rangeli	63%	100%
A0A3S7X4P6	Leishmania donovani	71%	100%
A0A3S7X6U2	Leishmania donovani	87%	100%
A4HJG9	Leishmania braziliensis	72%	100%
A4HJH1	Leishmania braziliensis	72%	100%
A4HJI4	Leishmania braziliensis	71%	100%
A4I933	Leishmania infantum	86%	100%
B2KZE7	Corynebacterium striatum	29%	100%
C9ZQ48	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	99%
D0A536	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	57%	99%
E9B1Y8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	75%	100%
E9B201	Leishmania mexicana (strain MHOM/GT/2001/U1103)	70%	100%
E9B3Z8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	86%	100%
P43616	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	98%
Q3ZC84	Bos taurus	27%	99%
Q4Q426	Leishmania major	86%	100%
Q4Q660	Leishmania major	72%	100%
Q4Q673	Leishmania major	72%	100%
Q5AKA5	Candida albicans (strain SC5314 / ATCC MYA-2876)	26%	97%
Q5R432	Pongo abelii	28%	99%
Q6Q0N1	Rattus norvegicus	26%	99%
Q96KP4	Homo sapiens	28%	99%
Q9D1A2	Mus musculus	25%	99%
Q9P6I2	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	24%	99%
V5BHA2	Trypanosoma cruzi	62%	99%