Putative trans-splicing factor

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

trans-splicing factor, putative

Species:

Leishmania braziliensis

UniProt:

A4HL50_LEIBR

TriTrypDb:

LbrM.33.0140 , LBRM2903_330006300

Length:

341

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0030532	small nuclear ribonucleoprotein complex	3	12
GO:0032991	protein-containing complex	1	12
GO:0046540	U4/U6 x U5 tri-snRNP complex	6	12
GO:0097525	spliceosomal snRNP complex	4	12
GO:0097526	spliceosomal tri-snRNP complex	5	12
GO:0120114	Sm-like protein family complex	2	12
GO:0140513	nuclear protein-containing complex	2	12
GO:1990904	ribonucleoprotein complex	2	12
GO:0005654	nucleoplasm	2	1
GO:0005681	spliceosomal complex	3	1
GO:0005687	U4 snRNP	5	1
GO:0071011	precatalytic spliceosome	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HL50

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HL50

Function

Biological processes
Term	Name	Level	Count
GO:0000244	spliceosomal tri-snRNP complex assembly	8	12
GO:0000387	spliceosomal snRNP assembly	7	12
GO:0009987	cellular process	1	12
GO:0016043	cellular component organization	3	12
GO:0022607	cellular component assembly	4	12
GO:0022618	ribonucleoprotein complex assembly	6	12
GO:0043933	protein-containing complex organization	4	12
GO:0065003	protein-containing complex assembly	5	12
GO:0071826	ribonucleoprotein complex subunit organization	5	12
GO:0071840	cellular component organization or biogenesis	2	12
GO:0000375	RNA splicing, via transesterification reactions	8	1
GO:0000377	RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	9	1
GO:0000398	mRNA splicing, via spliceosome	8	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006397	mRNA processing	7	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0008380	RNA splicing	7	1
GO:0016070	RNA metabolic process	5	1
GO:0016071	mRNA metabolic process	6	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	244	246	PF00675	0.358
CLV_NRD_NRD_1	294	296	PF00675	0.253
CLV_NRD_NRD_1	306	308	PF00675	0.253
CLV_NRD_NRD_1	332	334	PF00675	0.565
CLV_NRD_NRD_1	338	340	PF00675	0.556
CLV_NRD_NRD_1	56	58	PF00675	0.514
CLV_PCSK_FUR_1	304	308	PF00082	0.339
CLV_PCSK_FUR_1	335	339	PF00082	0.584
CLV_PCSK_KEX2_1	244	246	PF00082	0.294
CLV_PCSK_KEX2_1	294	296	PF00082	0.253
CLV_PCSK_KEX2_1	306	308	PF00082	0.253
CLV_PCSK_KEX2_1	332	334	PF00082	0.550
CLV_PCSK_KEX2_1	336	338	PF00082	0.547
CLV_PCSK_KEX2_1	56	58	PF00082	0.514
CLV_PCSK_PC1ET2_1	336	338	PF00082	0.550
CLV_PCSK_PC7_1	240	246	PF00082	0.358
CLV_PCSK_PC7_1	332	338	PF00082	0.562
CLV_PCSK_SKI1_1	126	130	PF00082	0.314
CLV_PCSK_SKI1_1	306	310	PF00082	0.248
DEG_Nend_UBRbox_2	1	3	PF02207	0.645
DEG_SPOP_SBC_1	160	164	PF00917	0.495
DOC_CYCLIN_RxL_1	272	282	PF00134	0.465
DOC_CYCLIN_yClb5_NLxxxL_5	203	212	PF00134	0.453
DOC_MAPK_gen_1	275	285	PF00069	0.558
DOC_MAPK_gen_1	96	103	PF00069	0.468
DOC_MAPK_JIP1_4	273	279	PF00069	0.465
DOC_MAPK_MEF2A_6	278	287	PF00069	0.558
DOC_MAPK_MEF2A_6	96	103	PF00069	0.539
DOC_MAPK_RevD_3	230	245	PF00069	0.558
DOC_PP2B_LxvP_1	317	320	PF13499	0.480
DOC_USP7_MATH_1	158	162	PF00917	0.453
DOC_WW_Pin1_4	166	171	PF00397	0.537
LIG_14-3-3_CanoR_1	278	284	PF00244	0.450
LIG_14-3-3_CanoR_1	294	303	PF00244	0.423
LIG_14-3-3_CanoR_1	306	313	PF00244	0.468
LIG_14-3-3_CanoR_1	47	51	PF00244	0.490
LIG_14-3-3_CanoR_1	56	65	PF00244	0.549
LIG_14-3-3_CterR_2	337	341	PF00244	0.627
LIG_Actin_WH2_2	310	325	PF00022	0.520
LIG_BRCT_BRCA1_1	107	111	PF00533	0.505
LIG_BRCT_BRCA1_1	217	221	PF00533	0.453
LIG_CtBP_PxDLS_1	170	174	PF00389	0.558
LIG_FHA_1	215	221	PF00498	0.453
LIG_FHA_1	254	260	PF00498	0.463
LIG_FHA_1	282	288	PF00498	0.487
LIG_FHA_1	41	47	PF00498	0.392
LIG_FHA_2	11	17	PF00498	0.660
LIG_FHA_2	140	146	PF00498	0.378
LIG_FHA_2	190	196	PF00498	0.447
LIG_FHA_2	296	302	PF00498	0.539
LIG_GBD_Chelix_1	140	148	PF00786	0.240
LIG_LIR_Gen_1	121	131	PF02991	0.479
LIG_LIR_Gen_1	136	144	PF02991	0.472
LIG_LIR_Gen_1	76	84	PF02991	0.429
LIG_LIR_LC3C_4	150	155	PF02991	0.539
LIG_LIR_Nem_3	108	114	PF02991	0.490
LIG_LIR_Nem_3	121	127	PF02991	0.479
LIG_LIR_Nem_3	136	140	PF02991	0.472
LIG_LIR_Nem_3	192	196	PF02991	0.465
LIG_LIR_Nem_3	297	303	PF02991	0.540
LIG_LIR_Nem_3	76	82	PF02991	0.423
LIG_MYND_1	270	274	PF01753	0.478
LIG_RPA_C_Fungi	284	296	PF08784	0.391
LIG_RPA_C_Fungi	93	105	PF08784	0.426
LIG_SH2_CRK	124	128	PF00017	0.306
LIG_SH2_CRK	36	40	PF00017	0.469
LIG_SH2_CRK	79	83	PF00017	0.413
LIG_SH2_NCK_1	36	40	PF00017	0.532
LIG_SH2_SRC	79	82	PF00017	0.501
LIG_SH2_STAP1	107	111	PF00017	0.363
LIG_SH2_STAP1	124	128	PF00017	0.294
LIG_SH2_STAP1	61	65	PF00017	0.480
LIG_SH2_STAT5	196	199	PF00017	0.301
LIG_SH2_STAT5	50	53	PF00017	0.496
LIG_SH2_STAT5	81	84	PF00017	0.485
LIG_SUMO_SIM_anti_2	150	156	PF11976	0.394
LIG_SUMO_SIM_anti_2	24	32	PF11976	0.625
LIG_TRAF2_1	13	16	PF00917	0.693
LIG_TRAF2_1	169	172	PF00917	0.340
LIG_TRAF2_1	179	182	PF00917	0.292
LIG_TRAF2_1	19	22	PF00917	0.654
LIG_TYR_ITIM	122	127	PF00017	0.306
LIG_TYR_ITIM	34	39	PF00017	0.483
LIG_TYR_ITSM	296	303	PF00017	0.426
MOD_CK1_1	139	145	PF00069	0.265
MOD_CK1_1	161	167	PF00069	0.357
MOD_CK1_1	243	249	PF00069	0.413
MOD_CK1_1	49	55	PF00069	0.451
MOD_CK1_1	71	77	PF00069	0.513
MOD_CK1_1	83	89	PF00069	0.471
MOD_CK2_1	10	16	PF00069	0.690
MOD_CK2_1	161	167	PF00069	0.324
MOD_CK2_1	189	195	PF00069	0.306
MOD_CK2_1	295	301	PF00069	0.306
MOD_CK2_1	35	41	PF00069	0.439
MOD_CK2_1	56	62	PF00069	0.456
MOD_Cter_Amidation	333	336	PF01082	0.596
MOD_GlcNHglycan	163	166	PF01048	0.459
MOD_GlcNHglycan	223	226	PF01048	0.435
MOD_GlcNHglycan	248	251	PF01048	0.327
MOD_GlcNHglycan	51	54	PF01048	0.401
MOD_GSK3_1	135	142	PF00069	0.449
MOD_GSK3_1	211	218	PF00069	0.306
MOD_GSK3_1	253	260	PF00069	0.352
MOD_GSK3_1	279	286	PF00069	0.352
MOD_GSK3_1	45	52	PF00069	0.384
MOD_GSK3_1	80	87	PF00069	0.487
MOD_N-GLC_1	246	251	PF02516	0.391
MOD_N-GLC_2	207	209	PF02516	0.306
MOD_NEK2_1	118	123	PF00069	0.338
MOD_NEK2_1	211	216	PF00069	0.302
MOD_NEK2_1	221	226	PF00069	0.306
MOD_NEK2_1	279	284	PF00069	0.347
MOD_NEK2_1	45	50	PF00069	0.400
MOD_NEK2_2	105	110	PF00069	0.453
MOD_PIKK_1	139	145	PF00454	0.301
MOD_PIKK_1	147	153	PF00454	0.291
MOD_PIKK_1	215	221	PF00454	0.306
MOD_PK_1	35	41	PF00069	0.439
MOD_PKA_1	294	300	PF00069	0.261
MOD_PKA_1	306	312	PF00069	0.408
MOD_PKA_1	56	62	PF00069	0.532
MOD_PKA_2	243	249	PF00069	0.396
MOD_PKA_2	294	300	PF00069	0.277
MOD_PKA_2	306	312	PF00069	0.408
MOD_PKA_2	46	52	PF00069	0.507
MOD_PKA_2	56	62	PF00069	0.532
MOD_PKB_1	304	312	PF00069	0.305
MOD_Plk_1	135	141	PF00069	0.340
MOD_Plk_1	171	177	PF00069	0.391
MOD_Plk_1	23	29	PF00069	0.521
MOD_Plk_1	61	67	PF00069	0.505
MOD_Plk_1	83	89	PF00069	0.391
MOD_Plk_1	94	100	PF00069	0.316
MOD_Plk_4	118	124	PF00069	0.353
MOD_Plk_4	136	142	PF00069	0.243
MOD_Plk_4	189	195	PF00069	0.306
MOD_Plk_4	228	234	PF00069	0.426
MOD_Plk_4	23	29	PF00069	0.590
MOD_Plk_4	46	52	PF00069	0.409
MOD_Plk_4	84	90	PF00069	0.443
MOD_ProDKin_1	166	172	PF00069	0.422
TRG_DiLeu_BaEn_1	41	46	PF01217	0.377
TRG_DiLeu_BaEn_4	181	187	PF01217	0.426
TRG_DiLeu_BaLyEn_6	199	204	PF01217	0.322
TRG_ENDOCYTIC_2	124	127	PF00928	0.306
TRG_ENDOCYTIC_2	300	303	PF00928	0.426
TRG_ENDOCYTIC_2	36	39	PF00928	0.468
TRG_ENDOCYTIC_2	79	82	PF00928	0.416
TRG_ER_diArg_1	306	308	PF00400	0.335
TRG_ER_diArg_1	332	335	PF00400	0.563
TRG_ER_diArg_1	337	339	PF00400	0.559
TRG_ER_diArg_1	55	57	PF00400	0.527
TRG_NLS_MonoCore_2	334	339	PF00514	0.597
TRG_NLS_MonoExtC_3	334	339	PF00514	0.575
TRG_NLS_MonoExtN_4	332	339	PF00514	0.577
TRG_Pf-PMV_PEXEL_1	166	171	PF00026	0.302
TRG_Pf-PMV_PEXEL_1	56	60	PF00026	0.524

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IKZ4	Leptomonas seymouri	89%	100%
A0A0N1PD49	Leptomonas seymouri	23%	80%
A0A0S4J686	Bodo saltans	55%	96%
A0A0S4JJS5	Bodo saltans	24%	81%
A0A1X0NUB3	Trypanosomatidae	25%	78%
A0A1X0P0H6	Trypanosomatidae	69%	96%
A0A3Q8IDJ5	Leishmania donovani	95%	100%
A0A3R7KY21	Trypanosoma rangeli	66%	96%
A0A3S5H6V9	Leishmania donovani	24%	77%
A4H8A6	Leishmania braziliensis	24%	77%
A4HWM6	Leishmania infantum	24%	77%
A4I8M9	Leishmania infantum	95%	100%
A6ZPE5	Saccharomyces cerevisiae (strain YJM789)	23%	67%
A7TIF5	Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17)	23%	66%
D0A4K7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	66%	96%
E9AQD3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	24%	76%
E9B3J1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
O04656	Arabidopsis thaliana	24%	76%
Q12499	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	67%
Q4Q4L0	Leishmania major	95%	100%
Q5U5C5	Xenopus laevis	29%	68%
Q6CKR8	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	23%	67%
Q6NVP6	Xenopus tropicalis	31%	68%
Q753I4	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	23%	66%
Q7SXM7	Danio rerio	32%	67%
Q8CCF0	Mus musculus	30%	68%
Q8RXN6	Arabidopsis thaliana	32%	70%
Q8WWY3	Homo sapiens	30%	68%
V5BFW3	Trypanosoma cruzi	68%	96%