DNA-directed primase/polymerase protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

PrimPol-like protein 1, putative

Species:

Leishmania braziliensis

UniProt:

A4HL39_LEIBR

TriTrypDb:

LbrM.33.0030 , LBRM2903_330005200

Length:

559

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005759	mitochondrial matrix	5	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HL39

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HL39

Function

Biological processes
Term	Name	Level	Count
GO:0000731	DNA synthesis involved in DNA repair	6	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006259	DNA metabolic process	4	11
GO:0006281	DNA repair	5	11
GO:0006301	postreplication repair	6	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006950	response to stress	2	11
GO:0006974	DNA damage response	4	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009059	macromolecule biosynthetic process	4	11
GO:0009987	cellular process	1	11
GO:0018130	heterocycle biosynthetic process	4	11
GO:0019438	aromatic compound biosynthetic process	4	11
GO:0019985	translesion synthesis	7	11
GO:0033554	cellular response to stress	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034654	nucleobase-containing compound biosynthetic process	4	11
GO:0042276	error-prone translesion synthesis	8	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	11
GO:0046483	heterocycle metabolic process	3	11
GO:0050896	response to stimulus	1	11
GO:0051716	cellular response to stimulus	2	11
GO:0071704	organic substance metabolic process	2	11
GO:0071897	DNA biosynthetic process	5	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901362	organic cyclic compound biosynthetic process	4	11
GO:1901576	organic substance biosynthetic process	3	11
GO:0006260	DNA replication	5	1
GO:0006261	DNA-templated DNA replication	6	1
GO:0006264	mitochondrial DNA replication	6	1
GO:0009314	response to radiation	3	1
GO:0009411	response to UV	5	1
GO:0009416	response to light stimulus	4	1
GO:0009628	response to abiotic stimulus	2	1
GO:0031297	replication fork processing	6	1
GO:0032042	mitochondrial DNA metabolic process	5	1
GO:0045005	DNA-templated DNA replication maintenance of fidelity	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0003887	DNA-directed DNA polymerase activity	5	11
GO:0003896	DNA primase activity	7	11
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	11
GO:0016740	transferase activity	2	11
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	11
GO:0016779	nucleotidyltransferase activity	4	11
GO:0034061	DNA polymerase activity	4	11
GO:0034062	5'-3' RNA polymerase activity	5	11
GO:0097747	RNA polymerase activity	4	11
GO:0140097	catalytic activity, acting on DNA	3	11
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:0003682	chromatin binding	2	1
GO:0005488	binding	1	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	250	254	PF00656	0.392
CLV_NRD_NRD_1	189	191	PF00675	0.600
CLV_NRD_NRD_1	198	200	PF00675	0.415
CLV_NRD_NRD_1	204	206	PF00675	0.383
CLV_NRD_NRD_1	352	354	PF00675	0.460
CLV_NRD_NRD_1	530	532	PF00675	0.356
CLV_NRD_NRD_1	75	77	PF00675	0.554
CLV_NRD_NRD_1	86	88	PF00675	0.634
CLV_PCSK_KEX2_1	189	191	PF00082	0.600
CLV_PCSK_KEX2_1	198	200	PF00082	0.415
CLV_PCSK_KEX2_1	204	206	PF00082	0.383
CLV_PCSK_KEX2_1	352	354	PF00082	0.456
CLV_PCSK_KEX2_1	529	531	PF00082	0.258
CLV_PCSK_KEX2_1	75	77	PF00082	0.546
CLV_PCSK_KEX2_1	88	90	PF00082	0.566
CLV_PCSK_PC1ET2_1	529	531	PF00082	0.249
CLV_PCSK_PC1ET2_1	88	90	PF00082	0.566
CLV_PCSK_SKI1_1	108	112	PF00082	0.340
CLV_PCSK_SKI1_1	119	123	PF00082	0.349
CLV_PCSK_SKI1_1	190	194	PF00082	0.613
DEG_APCC_DBOX_1	118	126	PF00400	0.466
DEG_APCC_DBOX_1	343	351	PF00400	0.391
DEG_Nend_Nbox_1	1	3	PF02207	0.399
DEG_SCF_FBW7_1	552	559	PF00400	0.448
DOC_ANK_TNKS_1	33	40	PF00023	0.373
DOC_ANK_TNKS_1	384	391	PF00023	0.277
DOC_CDC14_PxL_1	267	275	PF14671	0.284
DOC_CYCLIN_RxL_1	333	341	PF00134	0.577
DOC_CYCLIN_RxL_1	495	503	PF00134	0.448
DOC_MAPK_gen_1	137	146	PF00069	0.371
DOC_MAPK_gen_1	374	382	PF00069	0.344
DOC_MAPK_gen_1	515	524	PF00069	0.467
DOC_MAPK_JIP1_4	140	146	PF00069	0.235
DOC_MAPK_MEF2A_6	140	148	PF00069	0.362
DOC_MAPK_MEF2A_6	518	526	PF00069	0.492
DOC_PP1_RVXF_1	496	503	PF00149	0.436
DOC_PP2B_PxIxI_1	158	164	PF00149	0.391
DOC_PP4_FxxP_1	325	328	PF00568	0.382
DOC_USP7_MATH_1	235	239	PF00917	0.443
DOC_USP7_MATH_1	307	311	PF00917	0.386
DOC_USP7_MATH_1	403	407	PF00917	0.434
DOC_USP7_MATH_1	424	428	PF00917	0.439
DOC_USP7_MATH_1	437	441	PF00917	0.498
DOC_WW_Pin1_4	438	443	PF00397	0.547
DOC_WW_Pin1_4	544	549	PF00397	0.578
DOC_WW_Pin1_4	552	557	PF00397	0.544
DOC_WW_Pin1_4	98	103	PF00397	0.500
LIG_14-3-3_CanoR_1	204	214	PF00244	0.328
LIG_14-3-3_CanoR_1	344	348	PF00244	0.472
LIG_14-3-3_CanoR_1	430	438	PF00244	0.470
LIG_14-3-3_CanoR_1	488	492	PF00244	0.422
LIG_14-3-3_CanoR_1	498	503	PF00244	0.497
LIG_14-3-3_CanoR_1	530	534	PF00244	0.534
LIG_14-3-3_CanoR_1	75	80	PF00244	0.508
LIG_Actin_WH2_2	424	441	PF00022	0.379
LIG_APCC_ABBA_1	177	182	PF00400	0.292
LIG_APCC_ABBAyCdc20_2	257	263	PF00400	0.270
LIG_BRCT_BRCA1_1	106	110	PF00533	0.488
LIG_BRCT_BRCA1_1	403	407	PF00533	0.406
LIG_BRCT_BRCA1_1	50	54	PF00533	0.335
LIG_Clathr_ClatBox_1	178	182	PF01394	0.428
LIG_FHA_1	293	299	PF00498	0.442
LIG_FHA_2	455	461	PF00498	0.528
LIG_LIR_Gen_1	259	267	PF02991	0.410
LIG_LIR_Gen_1	404	415	PF02991	0.287
LIG_LIR_Gen_1	476	486	PF02991	0.306
LIG_LIR_Gen_1	92	102	PF02991	0.506
LIG_LIR_Nem_3	259	264	PF02991	0.420
LIG_LIR_Nem_3	404	410	PF02991	0.306
LIG_LIR_Nem_3	476	482	PF02991	0.279
LIG_LIR_Nem_3	92	97	PF02991	0.511
LIG_Pex14_1	331	335	PF04695	0.426
LIG_Pex14_2	261	265	PF04695	0.383
LIG_Pex14_2	301	305	PF04695	0.412
LIG_Pex14_2	50	54	PF04695	0.335
LIG_Pex14_2	8	12	PF04695	0.335
LIG_Rb_LxCxE_1	1	16	PF01857	0.359
LIG_REV1ctd_RIR_1	322	327	PF16727	0.363
LIG_SH2_CRK	479	483	PF00017	0.400
LIG_SH2_CRK	545	549	PF00017	0.531
LIG_SH2_NCK_1	479	483	PF00017	0.469
LIG_SH2_SRC	133	136	PF00017	0.362
LIG_SH2_SRC	145	148	PF00017	0.267
LIG_SH2_SRC	387	390	PF00017	0.389
LIG_SH2_STAP1	479	483	PF00017	0.419
LIG_SH2_STAT5	145	148	PF00017	0.305
LIG_SH2_STAT5	180	183	PF00017	0.492
LIG_SH2_STAT5	283	286	PF00017	0.299
LIG_SH2_STAT5	349	352	PF00017	0.444
LIG_SH2_STAT5	387	390	PF00017	0.422
LIG_SH2_STAT5	509	512	PF00017	0.471
LIG_SH2_STAT5	523	526	PF00017	0.416
LIG_SH3_1	545	551	PF00018	0.537
LIG_SH3_2	348	353	PF14604	0.318
LIG_SH3_3	327	333	PF00018	0.349
LIG_SH3_3	345	351	PF00018	0.341
LIG_SH3_3	377	383	PF00018	0.370
LIG_SH3_3	486	492	PF00018	0.478
LIG_SH3_3	545	551	PF00018	0.512
LIG_SUMO_SIM_anti_2	425	430	PF11976	0.449
LIG_SUMO_SIM_par_1	159	171	PF11976	0.621
LIG_TRAF2_1	171	174	PF00917	0.559
LIG_UBA3_1	428	434	PF00899	0.315
LIG_WRC_WIRS_1	236	241	PF05994	0.392
LIG_WRC_WIRS_1	499	504	PF05994	0.480
LIG_WW_2	548	551	PF00397	0.332
MOD_CDK_SPxK_1	438	444	PF00069	0.564
MOD_CK1_1	104	110	PF00069	0.398
MOD_CK1_1	120	126	PF00069	0.404
MOD_CK1_1	167	173	PF00069	0.585
MOD_CK1_1	294	300	PF00069	0.427
MOD_CK1_1	454	460	PF00069	0.664
MOD_CK2_1	120	126	PF00069	0.545
MOD_CK2_1	167	173	PF00069	0.691
MOD_CK2_1	470	476	PF00069	0.271
MOD_GlcNHglycan	249	252	PF01048	0.442
MOD_GlcNHglycan	55	58	PF01048	0.416
MOD_GSK3_1	164	171	PF00069	0.620
MOD_GSK3_1	243	250	PF00069	0.510
MOD_GSK3_1	287	294	PF00069	0.440
MOD_GSK3_1	418	425	PF00069	0.426
MOD_GSK3_1	451	458	PF00069	0.719
MOD_GSK3_1	477	484	PF00069	0.409
MOD_GSK3_1	487	494	PF00069	0.352
MOD_GSK3_1	500	507	PF00069	0.330
MOD_GSK3_1	552	559	PF00069	0.479
MOD_GSK3_1	58	65	PF00069	0.484
MOD_N-GLC_2	227	229	PF02516	0.393
MOD_NEK2_1	193	198	PF00069	0.397
MOD_NEK2_1	287	292	PF00069	0.472
MOD_NEK2_1	396	401	PF00069	0.466
MOD_NEK2_1	410	415	PF00069	0.292
MOD_NEK2_1	464	469	PF00069	0.472
MOD_NEK2_1	500	505	PF00069	0.300
MOD_NEK2_2	369	374	PF00069	0.331
MOD_PIKK_1	101	107	PF00454	0.465
MOD_PIKK_1	193	199	PF00454	0.304
MOD_PIKK_1	277	283	PF00454	0.327
MOD_PIKK_1	477	483	PF00454	0.449
MOD_PIKK_1	487	493	PF00454	0.495
MOD_PIKK_1	82	88	PF00454	0.729
MOD_PKA_1	529	535	PF00069	0.381
MOD_PKA_1	75	81	PF00069	0.485
MOD_PKA_2	188	194	PF00069	0.606
MOD_PKA_2	256	262	PF00069	0.411
MOD_PKA_2	343	349	PF00069	0.484
MOD_PKA_2	429	435	PF00069	0.426
MOD_PKA_2	487	493	PF00069	0.330
MOD_PKA_2	504	510	PF00069	0.482
MOD_PKA_2	529	535	PF00069	0.578
MOD_PKA_2	75	81	PF00069	0.470
MOD_PKB_1	241	249	PF00069	0.504
MOD_Plk_1	217	223	PF00069	0.461
MOD_Plk_1	336	342	PF00069	0.337
MOD_Plk_1	451	457	PF00069	0.722
MOD_Plk_1	464	470	PF00069	0.357
MOD_Plk_1	58	64	PF00069	0.547
MOD_Plk_1	92	98	PF00069	0.624
MOD_Plk_4	228	234	PF00069	0.327
MOD_Plk_4	235	241	PF00069	0.349
MOD_Plk_4	243	249	PF00069	0.386
MOD_Plk_4	256	262	PF00069	0.459
MOD_Plk_4	287	293	PF00069	0.489
MOD_Plk_4	376	382	PF00069	0.385
MOD_Plk_4	410	416	PF00069	0.365
MOD_Plk_4	424	430	PF00069	0.445
MOD_ProDKin_1	438	444	PF00069	0.550
MOD_ProDKin_1	544	550	PF00069	0.578
MOD_ProDKin_1	552	558	PF00069	0.555
MOD_ProDKin_1	98	104	PF00069	0.490
TRG_DiLeu_BaEn_1	174	179	PF01217	0.481
TRG_DiLeu_BaEn_1	92	97	PF01217	0.630
TRG_DiLeu_LyEn_5	268	273	PF01217	0.273
TRG_ENDOCYTIC_2	133	136	PF00928	0.292
TRG_ENDOCYTIC_2	145	148	PF00928	0.305
TRG_ENDOCYTIC_2	200	203	PF00928	0.489
TRG_ENDOCYTIC_2	332	335	PF00928	0.465
TRG_ENDOCYTIC_2	479	482	PF00928	0.404
TRG_ENDOCYTIC_2	523	526	PF00928	0.449
TRG_ER_diArg_1	198	200	PF00400	0.362
TRG_ER_diArg_1	203	205	PF00400	0.329
TRG_ER_diArg_1	351	353	PF00400	0.451
TRG_ER_diArg_1	87	90	PF00400	0.567
TRG_NLS_Bipartite_1	75	91	PF00514	0.656
TRG_NLS_MonoExtC_3	86	91	PF00514	0.563
TRG_Pf-PMV_PEXEL_1	336	341	PF00026	0.468

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PCE0	Leptomonas seymouri	56%	99%
A0A0S4IKK2	Bodo saltans	28%	84%
A0A3R7MZJ4	Trypanosoma rangeli	35%	86%
A0A3S5H7T6	Leishmania donovani	75%	100%
A4I8L9	Leishmania infantum	75%	100%
C9ZPU3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	92%
E9B3I0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	76%	100%
Q4Q4M0	Leishmania major	76%	100%
V5DB07	Trypanosoma cruzi	35%	91%