Cysteine protease

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Cysteine protease

Gene product:

AUT2/APG4/ATG4 cysteine peptidase, putative

Species:

Leishmania braziliensis

UniProt:

A4HL26_LEIBR

TriTrypDb:

LbrM.32.4130 , LBRM2903_320051100

Length:

398

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	11
GO:0110165	cellular anatomical entity	1	11

Expansion

Sequence features

A4HL26

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HL26

Function

Biological processes
Term	Name	Level	Count
GO:0000045	autophagosome assembly	6	11
GO:0006508	proteolysis	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006810	transport	3	11
GO:0006996	organelle organization	4	11
GO:0007033	vacuole organization	5	11
GO:0008104	protein localization	4	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0015031	protein transport	4	11
GO:0016043	cellular component organization	3	11
GO:0019538	protein metabolic process	3	11
GO:0022607	cellular component assembly	4	11
GO:0033036	macromolecule localization	2	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044238	primary metabolic process	2	11
GO:0045184	establishment of protein localization	3	11
GO:0051179	localization	1	11
GO:0051234	establishment of localization	2	11
GO:0051641	cellular localization	2	11
GO:0051697	protein delipidation	5	11
GO:0070727	cellular macromolecule localization	3	11
GO:0070925	organelle assembly	5	11
GO:0071702	organic substance transport	4	11
GO:0071704	organic substance metabolic process	2	11
GO:0071705	nitrogen compound transport	4	11
GO:0071840	cellular component organization or biogenesis	2	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1905037	autophagosome organization	6	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004175	endopeptidase activity	4	11
GO:0004197	cysteine-type endopeptidase activity	5	11
GO:0008233	peptidase activity	3	11
GO:0008234	cysteine-type peptidase activity	4	11
GO:0016787	hydrolase activity	2	11
GO:0140096	catalytic activity, acting on a protein	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	263	265	PF00675	0.183
CLV_NRD_NRD_1	296	298	PF00675	0.304
CLV_NRD_NRD_1	40	42	PF00675	0.629
CLV_PCSK_SKI1_1	264	268	PF00082	0.340
CLV_PCSK_SKI1_1	381	385	PF00082	0.486
CLV_PCSK_SKI1_1	71	75	PF00082	0.286
DEG_ODPH_VHL_1	224	236	PF01847	0.564
DOC_CKS1_1	36	41	PF01111	0.641
DOC_CYCLIN_RxL_1	68	77	PF00134	0.387
DOC_CYCLIN_yCln2_LP_2	216	222	PF00134	0.460
DOC_MAPK_gen_1	297	305	PF00069	0.557
DOC_MAPK_MEF2A_6	230	238	PF00069	0.459
DOC_MAPK_MEF2A_6	298	307	PF00069	0.564
DOC_MAPK_MEF2A_6	381	390	PF00069	0.485
DOC_PP1_RVXF_1	262	269	PF00149	0.540
DOC_PP1_RVXF_1	70	77	PF00149	0.512
DOC_PP2B_LxvP_1	216	219	PF13499	0.453
DOC_PP4_FxxP_1	268	271	PF00568	0.491
DOC_PP4_FxxP_1	36	39	PF00568	0.647
DOC_USP7_MATH_1	277	281	PF00917	0.512
DOC_USP7_MATH_1	86	90	PF00917	0.528
DOC_USP7_UBL2_3	156	160	PF12436	0.564
DOC_USP7_UBL2_3	42	46	PF12436	0.337
DOC_WW_Pin1_4	163	168	PF00397	0.479
DOC_WW_Pin1_4	341	346	PF00397	0.673
DOC_WW_Pin1_4	35	40	PF00397	0.622
DOC_WW_Pin1_4	388	393	PF00397	0.694
DOC_WW_Pin1_4	44	49	PF00397	0.528
LIG_14-3-3_CanoR_1	100	106	PF00244	0.564
LIG_14-3-3_CanoR_1	350	355	PF00244	0.768
LIG_14-3-3_CanoR_1	61	68	PF00244	0.278
LIG_BRCT_BRCA1_1	133	137	PF00533	0.484
LIG_BRCT_BRCA1_1	32	36	PF00533	0.635
LIG_EH_1	373	377	PF12763	0.524
LIG_EVH1_1	82	86	PF00568	0.564
LIG_FHA_1	100	106	PF00498	0.506
LIG_FHA_1	328	334	PF00498	0.309
LIG_FHA_1	45	51	PF00498	0.479
LIG_FHA_1	61	67	PF00498	0.300
LIG_FHA_2	309	315	PF00498	0.540
LIG_Integrin_RGD_1	291	293	PF01839	0.211
LIG_LIR_Apic_2	33	39	PF02991	0.649
LIG_LIR_Gen_1	134	145	PF02991	0.513
LIG_LIR_Gen_1	228	239	PF02991	0.471
LIG_LIR_Gen_1	62	73	PF02991	0.522
LIG_LIR_Nem_3	134	140	PF02991	0.513
LIG_LIR_Nem_3	255	259	PF02991	0.407
LIG_LIR_Nem_3	313	318	PF02991	0.474
LIG_LIR_Nem_3	330	334	PF02991	0.299
LIG_LIR_Nem_3	80	85	PF02991	0.471
LIG_LIR_Nem_3	92	98	PF02991	0.510
LIG_PTAP_UEV_1	140	145	PF05743	0.460
LIG_SH2_PTP2	119	122	PF00017	0.564
LIG_SH2_STAP1	161	165	PF00017	0.540
LIG_SH2_STAP1	231	235	PF00017	0.474
LIG_SH2_STAT5	119	122	PF00017	0.510
LIG_SH2_STAT5	273	276	PF00017	0.501
LIG_SH2_STAT5	278	281	PF00017	0.505
LIG_SH2_STAT5	308	311	PF00017	0.449
LIG_SH2_STAT5	75	78	PF00017	0.487
LIG_SH3_3	138	144	PF00018	0.460
LIG_SH3_3	326	332	PF00018	0.484
LIG_SH3_3	392	398	PF00018	0.716
LIG_SH3_3	80	86	PF00018	0.540
LIG_SUMO_SIM_anti_2	320	327	PF11976	0.298
LIG_SUMO_SIM_anti_2	47	54	PF11976	0.495
LIG_SUMO_SIM_par_1	47	54	PF11976	0.495
LIG_UBA3_1	234	242	PF00899	0.560
LIG_UBA3_1	67	72	PF00899	0.205
LIG_WW_2	83	86	PF00397	0.564
MOD_CDK_SPxK_1	35	41	PF00069	0.644
MOD_CDK_SPxxK_3	35	42	PF00069	0.642
MOD_CK1_1	163	169	PF00069	0.527
MOD_CK1_1	280	286	PF00069	0.495
MOD_CK1_1	327	333	PF00069	0.407
MOD_CK1_1	337	343	PF00069	0.507
MOD_CK1_1	77	83	PF00069	0.531
MOD_CK2_1	163	169	PF00069	0.528
MOD_Cter_Amidation	262	265	PF01082	0.183
MOD_GlcNHglycan	141	144	PF01048	0.262
MOD_GlcNHglycan	2	5	PF01048	0.651
MOD_GlcNHglycan	359	362	PF01048	0.636
MOD_GlcNHglycan	53	56	PF01048	0.408
MOD_GlcNHglycan	57	60	PF01048	0.424
MOD_GSK3_1	276	283	PF00069	0.465
MOD_GSK3_1	334	341	PF00069	0.453
MOD_GSK3_1	51	58	PF00069	0.460
MOD_GSK3_1	86	93	PF00069	0.499
MOD_N-GLC_1	131	136	PF02516	0.183
MOD_N-GLC_1	386	391	PF02516	0.482
MOD_N-GLC_1	86	91	PF02516	0.270
MOD_NEK2_1	105	110	PF00069	0.520
MOD_NEK2_1	386	391	PF00069	0.482
MOD_NEK2_1	74	79	PF00069	0.460
MOD_NEK2_1	98	103	PF00069	0.490
MOD_PIKK_1	99	105	PF00454	0.540
MOD_PKA_2	60	66	PF00069	0.435
MOD_PKA_2	99	105	PF00069	0.538
MOD_Plk_1	131	137	PF00069	0.383
MOD_Plk_1	386	392	PF00069	0.479
MOD_Plk_4	160	166	PF00069	0.500
MOD_Plk_4	21	27	PF00069	0.585
MOD_Plk_4	230	236	PF00069	0.432
MOD_Plk_4	252	258	PF00069	0.540
MOD_Plk_4	283	289	PF00069	0.514
MOD_Plk_4	324	330	PF00069	0.449
MOD_ProDKin_1	163	169	PF00069	0.479
MOD_ProDKin_1	341	347	PF00069	0.674
MOD_ProDKin_1	35	41	PF00069	0.618
MOD_ProDKin_1	388	394	PF00069	0.691
MOD_ProDKin_1	44	50	PF00069	0.521
MOD_SUMO_rev_2	123	131	PF00179	0.383
TRG_DiLeu_BaLyEn_6	329	334	PF01217	0.385
TRG_ENDOCYTIC_2	161	164	PF00928	0.540
TRG_ENDOCYTIC_2	231	234	PF00928	0.460
TRG_ENDOCYTIC_2	315	318	PF00928	0.564
TRG_NES_CRM1_1	128	139	PF08389	0.542
TRG_Pf-PMV_PEXEL_1	197	201	PF00026	0.364
TRG_Pf-PMV_PEXEL_1	41	45	PF00026	0.337

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A098DRK7	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	22%	85%
A0A0N1IIY5	Leptomonas seymouri	67%	100%
A0A0S4JSC7	Bodo saltans	27%	100%
A0A1X0NS04	Trypanosomatidae	37%	100%
A0A1X0P2H1	Trypanosomatidae	25%	100%
A0A3S7X6B3	Leishmania donovani	77%	100%
A0A422NI42	Trypanosoma rangeli	40%	100%
A1CJ08	Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107)	24%	100%
A2Q1V6	Medicago truncatula	23%	82%
A2QY50	Aspergillus niger (strain CBS 513.88 / FGSC A1513)	25%	99%
A2XHJ5	Oryza sativa subsp. indica	22%	84%
A4I8K7	Leishmania infantum	77%	100%
A5DEF7	Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324)	26%	99%
A5DSB4	Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239)	22%	76%
C9ZQI0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	100%
D0AAK8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	100%
E2RDP2	Canis lupus familiaris	25%	84%
E9B3G7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	75%	100%
Q1E5M9	Coccidioides immitis (strain RS)	25%	92%
Q2HH40	Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970)	23%	89%
Q2U5B0	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	27%	98%
Q2XPP4	Oryza sativa subsp. indica	23%	83%
Q4Q4N3	Leishmania major	76%	100%
Q4U3V5	Cryphonectria parasitica	24%	87%
Q523C3	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	24%	81%
Q5B7L0	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	23%	99%
Q5XH30	Xenopus laevis	23%	88%
Q684M2	Sus scrofa	26%	85%
Q68EP9	Xenopus tropicalis	23%	88%
Q68FJ9	Xenopus laevis	23%	85%
Q6CH28	Yarrowia lipolytica (strain CLIB 122 / E 150)	24%	73%
Q6FP20	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	24%	82%
Q6PZ02	Gallus gallus	23%	100%
Q75E61	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	24%	76%
Q75KP8	Oryza sativa subsp. japonica	23%	84%
Q7XPW8	Oryza sativa subsp. japonica	23%	83%
Q811C2	Mus musculus	24%	87%
Q86TL0	Homo sapiens	26%	84%
Q8BGV9	Mus musculus	25%	84%
Q8S929	Arabidopsis thaliana	26%	85%
Q96DT6	Homo sapiens	23%	87%
Q9M1Y0	Arabidopsis thaliana	23%	83%
V5AXZ8	Trypanosoma cruzi	38%	100%
V5BGZ3	Trypanosoma cruzi	25%	100%
W0TGM7	Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275)	23%	88%