tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Methyltransferase TYW3, putative

Species:

Leishmania braziliensis

UniProt:

A4HL12_LEIBR

TriTrypDb:

LbrM.32.3990 , LBRM2903_320049500

Length:

280

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HL12

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HL12

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006399	tRNA metabolic process	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008033	tRNA processing	8	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0032259	methylation	2	12
GO:0034470	ncRNA processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0001510	RNA methylation	4	1
GO:0006400	tRNA modification	6	1
GO:0009451	RNA modification	5	1
GO:0030488	tRNA methylation	5	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0008168	methyltransferase activity	4	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0008173	RNA methyltransferase activity	4	1
GO:0008175	tRNA methyltransferase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	271	275	PF00656	0.411
CLV_NRD_NRD_1	246	248	PF00675	0.293
CLV_NRD_NRD_1	263	265	PF00675	0.573
CLV_NRD_NRD_1	86	88	PF00675	0.320
CLV_PCSK_FUR_1	244	248	PF00082	0.417
CLV_PCSK_FUR_1	86	90	PF00082	0.451
CLV_PCSK_KEX2_1	244	246	PF00082	0.306
CLV_PCSK_KEX2_1	263	265	PF00082	0.588
CLV_PCSK_KEX2_1	277	279	PF00082	0.364
CLV_PCSK_KEX2_1	86	88	PF00082	0.314
CLV_PCSK_PC1ET2_1	277	279	PF00082	0.421
CLV_PCSK_PC1ET2_1	88	90	PF00082	0.304
CLV_PCSK_PC7_1	273	279	PF00082	0.417
DEG_Nend_UBRbox_1	1	4	PF02207	0.374
DEG_SIAH_1	65	73	PF03145	0.199
DEG_SPOP_SBC_1	41	45	PF00917	0.320
DOC_CYCLIN_yCln2_LP_2	195	201	PF00134	0.318
DOC_MAPK_MEF2A_6	137	146	PF00069	0.323
DOC_USP7_MATH_1	72	76	PF00917	0.435
DOC_USP7_MATH_1	80	84	PF00917	0.307
DOC_USP7_UBL2_3	186	190	PF12436	0.393
DOC_WW_Pin1_4	194	199	PF00397	0.366
DOC_WW_Pin1_4	211	216	PF00397	0.269
DOC_WW_Pin1_4	62	67	PF00397	0.451
LIG_14-3-3_CanoR_1	137	144	PF00244	0.466
LIG_14-3-3_CanoR_1	183	189	PF00244	0.360
LIG_14-3-3_CanoR_1	264	272	PF00244	0.668
LIG_14-3-3_CanoR_1	49	53	PF00244	0.204
LIG_14-3-3_CanoR_1	79	85	PF00244	0.199
LIG_Actin_WH2_2	151	168	PF00022	0.199
LIG_CaM_NSCaTE_8	223	230	PF13499	0.309
LIG_deltaCOP1_diTrp_1	121	126	PF00928	0.425
LIG_deltaCOP1_diTrp_1	209	218	PF00928	0.391
LIG_deltaCOP1_diTrp_1	221	226	PF00928	0.425
LIG_FHA_1	136	142	PF00498	0.326
LIG_FHA_1	190	196	PF00498	0.320
LIG_FHA_1	226	232	PF00498	0.331
LIG_FHA_1	36	42	PF00498	0.391
LIG_FHA_2	266	272	PF00498	0.420
LIG_LIR_Gen_1	220	231	PF02991	0.390
LIG_LIR_Nem_3	220	226	PF02991	0.304
LIG_LIR_Nem_3	36	40	PF02991	0.318
LIG_MYND_3	117	121	PF01753	0.420
LIG_PTB_Apo_2	31	38	PF02174	0.320
LIG_RPA_C_Fungi	241	253	PF08784	0.199
LIG_SH2_GRB2like	32	35	PF00017	0.425
LIG_SH2_SRC	32	35	PF00017	0.425
LIG_SH2_STAT5	32	35	PF00017	0.339
LIG_SH2_STAT5	40	43	PF00017	0.266
LIG_UBA3_1	150	159	PF00899	0.291
MOD_CK1_1	182	188	PF00069	0.325
MOD_CK1_1	59	65	PF00069	0.435
MOD_CK2_1	265	271	PF00069	0.427
MOD_CK2_1	79	85	PF00069	0.462
MOD_GlcNHglycan	170	173	PF01048	0.324
MOD_GlcNHglycan	175	178	PF01048	0.331
MOD_GlcNHglycan	57	61	PF01048	0.274
MOD_GSK3_1	108	115	PF00069	0.362
MOD_GSK3_1	131	138	PF00069	0.379
MOD_GSK3_1	173	180	PF00069	0.313
MOD_GSK3_1	189	196	PF00069	0.320
MOD_GSK3_1	35	42	PF00069	0.328
MOD_GSK3_1	4	11	PF00069	0.451
MOD_GSK3_1	55	62	PF00069	0.121
MOD_GSK3_1	93	100	PF00069	0.353
MOD_N-GLC_1	33	38	PF02516	0.393
MOD_NEK2_1	131	136	PF00069	0.199
MOD_NEK2_2	48	53	PF00069	0.204
MOD_NEK2_2	93	98	PF00069	0.362
MOD_PIKK_1	35	41	PF00454	0.451
MOD_PKA_1	88	94	PF00069	0.390
MOD_PKA_2	112	118	PF00069	0.335
MOD_PKA_2	136	142	PF00069	0.199
MOD_PKA_2	182	188	PF00069	0.328
MOD_PKA_2	272	278	PF00069	0.412
MOD_PKA_2	48	54	PF00069	0.220
MOD_PKA_2	88	94	PF00069	0.390
MOD_Plk_1	106	112	PF00069	0.425
MOD_Plk_1	14	20	PF00069	0.320
MOD_Plk_4	112	118	PF00069	0.211
MOD_Plk_4	131	137	PF00069	0.414
MOD_Plk_4	222	228	PF00069	0.387
MOD_Plk_4	233	239	PF00069	0.393
MOD_Plk_4	48	54	PF00069	0.212
MOD_Plk_4	72	78	PF00069	0.199
MOD_Plk_4	93	99	PF00069	0.356
MOD_ProDKin_1	194	200	PF00069	0.366
MOD_ProDKin_1	211	217	PF00069	0.269
MOD_ProDKin_1	62	68	PF00069	0.451
MOD_SUMO_for_1	144	147	PF00179	0.304
TRG_ER_diArg_1	244	247	PF00400	0.279
TRG_ER_diArg_1	263	265	PF00400	0.615
TRG_ER_diArg_1	86	89	PF00400	0.451
TRG_NLS_MonoExtN_4	86	91	PF00514	0.427

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PB24	Leptomonas seymouri	63%	99%
A0A1X0NNC3	Trypanosomatidae	27%	81%
A0A1X0NRX8	Trypanosomatidae	50%	93%
A0A3R7NUS4	Trypanosoma rangeli	51%	100%
A0A3S7X653	Leishmania donovani	79%	100%
A4I8J3	Leishmania infantum	79%	100%
D0AAM5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	94%
E9B3F3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	79%	100%
Q4Q4P7	Leishmania major	80%	100%
Q9UTA5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	24%	100%
V5DMF2	Trypanosoma cruzi	52%	100%