DUF4200 domain-containing protein

Quick info Localization Expansion Sequence features Structure Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Domain of unknown function (DUF4200), putative

Species:

Leishmania braziliensis

UniProt:

A4HKI8_LEIBR

TriTrypDb:

LbrM.32.2190 , LBRM2903_320028400

Length:

726

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 5

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005930	axoneme	2	1
GO:0110165	cellular anatomical entity	1	3
GO:0005929	cilium	4	2
GO:0031514	motile cilium	5	2
GO:0042995	cell projection	2	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0120025	plasma membrane bounded cell projection	3	2

Expansion

Sequence features

A4HKI8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HKI8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	106	108	PF00675	0.339
CLV_NRD_NRD_1	149	151	PF00675	0.245
CLV_NRD_NRD_1	15	17	PF00675	0.534
CLV_NRD_NRD_1	179	181	PF00675	0.245
CLV_NRD_NRD_1	482	484	PF00675	0.518
CLV_NRD_NRD_1	513	515	PF00675	0.680
CLV_NRD_NRD_1	552	554	PF00675	0.574
CLV_NRD_NRD_1	576	578	PF00675	0.709
CLV_NRD_NRD_1	644	646	PF00675	0.449
CLV_NRD_NRD_1	665	667	PF00675	0.447
CLV_NRD_NRD_1	698	700	PF00675	0.572
CLV_PCSK_FUR_1	663	667	PF00082	0.496
CLV_PCSK_KEX2_1	106	108	PF00082	0.245
CLV_PCSK_KEX2_1	117	119	PF00082	0.259
CLV_PCSK_KEX2_1	149	151	PF00082	0.258
CLV_PCSK_KEX2_1	15	17	PF00082	0.491
CLV_PCSK_KEX2_1	263	265	PF00082	0.692
CLV_PCSK_KEX2_1	453	455	PF00082	0.727
CLV_PCSK_KEX2_1	644	646	PF00082	0.450
CLV_PCSK_KEX2_1	665	667	PF00082	0.498
CLV_PCSK_KEX2_1	88	90	PF00082	0.245
CLV_PCSK_PC1ET2_1	117	119	PF00082	0.268
CLV_PCSK_PC1ET2_1	263	265	PF00082	0.665
CLV_PCSK_PC1ET2_1	453	455	PF00082	0.654
CLV_PCSK_PC1ET2_1	88	90	PF00082	0.245
CLV_PCSK_SKI1_1	158	162	PF00082	0.338
CLV_PCSK_SKI1_1	193	197	PF00082	0.338
CLV_PCSK_SKI1_1	220	224	PF00082	0.473
CLV_PCSK_SKI1_1	453	457	PF00082	0.495
CLV_PCSK_SKI1_1	555	559	PF00082	0.748
CLV_PCSK_SKI1_1	665	669	PF00082	0.497
CLV_PCSK_SKI1_1	8	12	PF00082	0.575
DEG_Nend_UBRbox_2	1	3	PF02207	0.398
DOC_CYCLIN_RxL_1	217	227	PF00134	0.468
DOC_CYCLIN_RxL_1	5	13	PF00134	0.577
DOC_CYCLIN_yCln2_LP_2	621	627	PF00134	0.522
DOC_MAPK_gen_1	130	139	PF00069	0.503
DOC_MAPK_gen_1	156	165	PF00069	0.445
DOC_MAPK_gen_1	180	186	PF00069	0.445
DOC_MAPK_gen_1	263	271	PF00069	0.729
DOC_MAPK_gen_1	577	583	PF00069	0.506
DOC_USP7_MATH_1	326	330	PF00917	0.447
DOC_USP7_MATH_1	457	461	PF00917	0.778
DOC_USP7_MATH_1	508	512	PF00917	0.460
DOC_USP7_MATH_1	534	538	PF00917	0.729
DOC_USP7_MATH_1	544	548	PF00917	0.767
DOC_USP7_MATH_1	58	62	PF00917	0.528
DOC_USP7_UBL2_3	634	638	PF12436	0.483
DOC_USP7_UBL2_3	691	695	PF12436	0.603
DOC_USP7_UBL2_3	696	700	PF12436	0.601
DOC_WW_Pin1_4	41	46	PF00397	0.457
DOC_WW_Pin1_4	475	480	PF00397	0.561
LIG_14-3-3_CanoR_1	264	270	PF00244	0.529
LIG_14-3-3_CanoR_1	37	43	PF00244	0.598
LIG_14-3-3_CanoR_1	466	474	PF00244	0.776
LIG_14-3-3_CanoR_1	514	522	PF00244	0.767
LIG_14-3-3_CanoR_1	532	537	PF00244	0.763
LIG_14-3-3_CanoR_1	545	551	PF00244	0.583
LIG_14-3-3_CanoR_1	553	561	PF00244	0.599
LIG_14-3-3_CanoR_1	577	582	PF00244	0.605
LIG_14-3-3_CanoR_1	684	689	PF00244	0.650
LIG_14-3-3_CanoR_1	95	105	PF00244	0.538
LIG_Actin_WH2_2	489	507	PF00022	0.675
LIG_CtBP_PxDLS_1	479	483	PF00389	0.406
LIG_FHA_1	160	166	PF00498	0.538
LIG_FHA_1	216	222	PF00498	0.461
LIG_FHA_1	319	325	PF00498	0.468
LIG_FHA_1	404	410	PF00498	0.509
LIG_FHA_1	411	417	PF00498	0.520
LIG_FHA_1	546	552	PF00498	0.692
LIG_FHA_1	578	584	PF00498	0.507
LIG_FHA_2	476	482	PF00498	0.549
LIG_FHA_2	524	530	PF00498	0.717
LIG_FHA_2	606	612	PF00498	0.438
LIG_FHA_2	78	84	PF00498	0.575
LIG_HCF-1_HBM_1	303	306	PF13415	0.502
LIG_Integrin_RGD_1	16	18	PF01839	0.449
LIG_LIR_Gen_1	131	139	PF02991	0.447
LIG_LIR_Gen_1	303	313	PF02991	0.484
LIG_LIR_Gen_1	361	371	PF02991	0.399
LIG_LIR_LC3C_4	547	552	PF02991	0.528
LIG_LIR_Nem_3	131	136	PF02991	0.447
LIG_LIR_Nem_3	361	366	PF02991	0.380
LIG_LIR_Nem_3	615	620	PF02991	0.445
LIG_PCNA_yPIPBox_3	395	405	PF02747	0.361
LIG_RPA_C_Fungi	227	239	PF08784	0.444
LIG_SH2_CRK	206	210	PF00017	0.371
LIG_SH2_GRB2like	620	623	PF00017	0.419
LIG_SH2_STAP1	71	75	PF00017	0.658
LIG_SH2_STAT5	620	623	PF00017	0.460
LIG_SH3_3	39	45	PF00018	0.621
LIG_SH3_3	429	435	PF00018	0.432
LIG_SH3_3	538	544	PF00018	0.580
LIG_SH3_3	686	692	PF00018	0.682
LIG_SH3_4	691	698	PF00018	0.429
LIG_SUMO_SIM_anti_2	605	611	PF11976	0.428
LIG_SUMO_SIM_anti_2	629	634	PF11976	0.539
LIG_SUMO_SIM_par_1	579	584	PF11976	0.507
LIG_SUMO_SIM_par_1	602	608	PF11976	0.453
LIG_TRAF2_1	297	300	PF00917	0.591
LIG_TRAF2_1	308	311	PF00917	0.402
LIG_TRAF2_1	478	481	PF00917	0.620
LIG_TRAF2_1	654	657	PF00917	0.568
LIG_TRAF2_2	593	598	PF00917	0.503
LIG_UBA3_1	606	613	PF00899	0.421
MOD_CK1_1	320	326	PF00069	0.457
MOD_CK1_1	41	47	PF00069	0.669
MOD_CK1_1	447	453	PF00069	0.744
MOD_CK1_1	510	516	PF00069	0.577
MOD_CK1_1	562	568	PF00069	0.750
MOD_CK1_1	687	693	PF00069	0.736
MOD_CK1_1	69	75	PF00069	0.548
MOD_CK1_1	711	717	PF00069	0.570
MOD_CK1_1	97	103	PF00069	0.404
MOD_CK2_1	137	143	PF00069	0.339
MOD_CK2_1	170	176	PF00069	0.362
MOD_CK2_1	304	310	PF00069	0.463
MOD_CK2_1	340	346	PF00069	0.410
MOD_CK2_1	412	418	PF00069	0.574
MOD_CK2_1	475	481	PF00069	0.609
MOD_CK2_1	605	611	PF00069	0.461
MOD_Cter_Amidation	261	264	PF01082	0.543
MOD_Cter_Amidation	575	578	PF01082	0.505
MOD_GlcNHglycan	168	171	PF01048	0.283
MOD_GlcNHglycan	2	5	PF01048	0.561
MOD_GlcNHglycan	232	235	PF01048	0.464
MOD_GlcNHglycan	51	54	PF01048	0.578
MOD_GlcNHglycan	569	572	PF01048	0.714
MOD_GlcNHglycan	574	577	PF01048	0.642
MOD_GlcNHglycan	61	64	PF01048	0.537
MOD_GlcNHglycan	67	71	PF01048	0.503
MOD_GSK3_1	166	173	PF00069	0.362
MOD_GSK3_1	37	44	PF00069	0.575
MOD_GSK3_1	440	447	PF00069	0.686
MOD_GSK3_1	453	460	PF00069	0.602
MOD_GSK3_1	49	56	PF00069	0.618
MOD_GSK3_1	504	511	PF00069	0.678
MOD_GSK3_1	513	520	PF00069	0.795
MOD_GSK3_1	528	535	PF00069	0.707
MOD_GSK3_1	555	562	PF00069	0.748
MOD_GSK3_1	572	579	PF00069	0.635
MOD_GSK3_1	65	72	PF00069	0.632
MOD_GSK3_1	75	82	PF00069	0.596
MOD_N-GLC_1	441	446	PF02516	0.505
MOD_N-GLC_1	475	480	PF02516	0.439
MOD_N-GLC_1	708	713	PF02516	0.617
MOD_NEK2_1	137	142	PF00069	0.283
MOD_NEK2_1	195	200	PF00069	0.251
MOD_NEK2_1	205	210	PF00069	0.439
MOD_NEK2_1	38	43	PF00069	0.619
MOD_NEK2_1	412	417	PF00069	0.473
MOD_NEK2_1	504	509	PF00069	0.652
MOD_NEK2_1	668	673	PF00069	0.460
MOD_NEK2_1	708	713	PF00069	0.553
MOD_NEK2_1	96	101	PF00069	0.487
MOD_NEK2_2	523	528	PF00069	0.516
MOD_PIKK_1	270	276	PF00454	0.619
MOD_PIKK_1	400	406	PF00454	0.464
MOD_PIKK_1	534	540	PF00454	0.651
MOD_PIKK_1	562	568	PF00454	0.770
MOD_PIKK_1	668	674	PF00454	0.493
MOD_PK_1	532	538	PF00069	0.532
MOD_PKA_1	166	172	PF00069	0.283
MOD_PKA_1	453	459	PF00069	0.498
MOD_PKA_1	514	520	PF00069	0.558
MOD_PKA_1	553	559	PF00069	0.656
MOD_PKA_1	577	583	PF00069	0.587
MOD_PKA_2	453	459	PF00069	0.628
MOD_PKA_2	465	471	PF00069	0.524
MOD_PKA_2	513	519	PF00069	0.740
MOD_PKA_2	544	550	PF00069	0.586
MOD_PKA_2	576	582	PF00069	0.795
MOD_PKA_2	94	100	PF00069	0.395
MOD_Plk_1	326	332	PF00069	0.339
MOD_Plk_1	486	492	PF00069	0.512
MOD_Plk_1	597	603	PF00069	0.728
MOD_Plk_2-3	605	611	PF00069	0.442
MOD_Plk_4	397	403	PF00069	0.362
MOD_Plk_4	546	552	PF00069	0.522
MOD_Plk_4	605	611	PF00069	0.428
MOD_ProDKin_1	41	47	PF00069	0.459
MOD_ProDKin_1	475	481	PF00069	0.553
MOD_SUMO_for_1	336	339	PF00179	0.428
MOD_SUMO_rev_2	104	112	PF00179	0.375
MOD_SUMO_rev_2	605	614	PF00179	0.425
MOD_SUMO_rev_2	690	698	PF00179	0.642
TRG_DiLeu_BaEn_3	367	373	PF01217	0.390
TRG_DiLeu_BaLyEn_6	671	676	PF01217	0.403
TRG_ENDOCYTIC_2	192	195	PF00928	0.283
TRG_ENDOCYTIC_2	206	209	PF00928	0.356
TRG_ENDOCYTIC_2	306	309	PF00928	0.477
TRG_ER_diArg_1	105	107	PF00400	0.203
TRG_ER_diArg_1	14	16	PF00400	0.552
TRG_ER_diArg_1	148	150	PF00400	0.301
TRG_ER_diArg_1	663	666	PF00400	0.473
TRG_ER_diArg_1	683	686	PF00400	0.390
TRG_NLS_Bipartite_1	166	184	PF00514	0.413
TRG_NLS_MonoExtC_3	179	185	PF00514	0.203
TRG_Pf-PMV_PEXEL_1	106	110	PF00026	0.283
TRG_Pf-PMV_PEXEL_1	619	623	PF00026	0.534

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEP4	Leptomonas seymouri	55%	94%
A0A1X0NUV9	Trypanosomatidae	39%	100%
A0A3Q8IG83	Leishmania donovani	74%	99%
A0A422NB67	Trypanosoma rangeli	39%	100%
A4I820	Leishmania infantum	74%	99%
D0AA86	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	100%
E9B2X8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	73%	98%
Q4Q578	Leishmania major	74%	100%
V5DCC5	Trypanosoma cruzi	38%	100%