DNA polymerase alpha subunit B

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA polymerase alpha/epsilon subunit B, putative

Species:

Leishmania braziliensis

UniProt:

A4HKF9_LEIBR

TriTrypDb:

LbrM.32.1890 , LBRM2903_320025300

Length:

728

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	11
GO:0043226	organelle	2	11
GO:0043227	membrane-bounded organelle	3	11
GO:0043229	intracellular organelle	3	11
GO:0043231	intracellular membrane-bounded organelle	4	11
GO:0110165	cellular anatomical entity	1	11
GO:0000428	DNA-directed RNA polymerase complex	4	1
GO:0005658	alpha DNA polymerase:primase complex	4	1
GO:0030880	RNA polymerase complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0042575	DNA polymerase complex	3	1
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	1
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	1
GO:0140513	nuclear protein-containing complex	2	1
GO:0140535	intracellular protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1990234	transferase complex	3	1

Expansion

Sequence features

A4HKF9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HKF9

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006260	DNA replication	5	11
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006270	DNA replication initiation	5	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003677	DNA binding	4	12
GO:0005488	binding	1	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0003824	catalytic activity	1	1
GO:0016740	transferase activity	2	1
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	1
GO:0016779	nucleotidyltransferase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	559	563	PF00656	0.798
CLV_C14_Caspase3-7	660	664	PF00656	0.694
CLV_NRD_NRD_1	343	345	PF00675	0.363
CLV_NRD_NRD_1	492	494	PF00675	0.343
CLV_NRD_NRD_1	70	72	PF00675	0.513
CLV_NRD_NRD_1	709	711	PF00675	0.538
CLV_PCSK_KEX2_1	343	345	PF00082	0.363
CLV_PCSK_KEX2_1	492	494	PF00082	0.344
CLV_PCSK_KEX2_1	597	599	PF00082	0.656
CLV_PCSK_KEX2_1	709	711	PF00082	0.538
CLV_PCSK_PC1ET2_1	597	599	PF00082	0.656
CLV_PCSK_SKI1_1	103	107	PF00082	0.537
CLV_PCSK_SKI1_1	173	177	PF00082	0.388
CLV_PCSK_SKI1_1	28	32	PF00082	0.647
CLV_PCSK_SKI1_1	283	287	PF00082	0.500
CLV_PCSK_SKI1_1	299	303	PF00082	0.438
CLV_PCSK_SKI1_1	308	312	PF00082	0.700
CLV_PCSK_SKI1_1	351	355	PF00082	0.383
CLV_PCSK_SKI1_1	381	385	PF00082	0.384
CLV_PCSK_SKI1_1	413	417	PF00082	0.374
CLV_PCSK_SKI1_1	420	424	PF00082	0.296
CLV_PCSK_SKI1_1	597	601	PF00082	0.652
CLV_PCSK_SKI1_1	79	83	PF00082	0.411
DEG_APCC_DBOX_1	172	180	PF00400	0.320
DEG_APCC_DBOX_1	282	290	PF00400	0.539
DEG_APCC_DBOX_1	380	388	PF00400	0.449
DEG_APCC_DBOX_1	412	420	PF00400	0.423
DEG_APCC_DBOX_1	597	605	PF00400	0.538
DEG_Nend_UBRbox_3	1	3	PF02207	0.676
DEG_SPOP_SBC_1	563	567	PF00917	0.680
DOC_CDC14_PxL_1	517	525	PF14671	0.561
DOC_CKS1_1	30	35	PF01111	0.621
DOC_CYCLIN_RxL_1	25	32	PF00134	0.476
DOC_CYCLIN_RxL_1	417	425	PF00134	0.449
DOC_MAPK_MEF2A_6	247	254	PF00069	0.327
DOC_MAPK_MEF2A_6	518	525	PF00069	0.566
DOC_MAPK_MEF2A_6	8	17	PF00069	0.581
DOC_MAPK_NFAT4_5	247	255	PF00069	0.322
DOC_MAPK_RevD_3	694	710	PF00069	0.509
DOC_PP1_RVXF_1	331	337	PF00149	0.420
DOC_PP2B_LxvP_1	503	506	PF13499	0.363
DOC_PP2B_LxvP_1	624	627	PF13499	0.760
DOC_PP4_FxxP_1	129	132	PF00568	0.448
DOC_PP4_FxxP_1	294	297	PF00568	0.399
DOC_PP4_FxxP_1	371	374	PF00568	0.449
DOC_USP7_MATH_1	186	190	PF00917	0.702
DOC_USP7_MATH_1	203	207	PF00917	0.779
DOC_USP7_MATH_1	374	378	PF00917	0.435
DOC_USP7_MATH_1	487	491	PF00917	0.418
DOC_USP7_MATH_1	541	545	PF00917	0.662
DOC_USP7_MATH_1	563	567	PF00917	0.750
DOC_USP7_MATH_1	570	574	PF00917	0.615
DOC_USP7_MATH_1	584	588	PF00917	0.670
DOC_USP7_MATH_1	671	675	PF00917	0.533
DOC_WW_Pin1_4	29	34	PF00397	0.622
DOC_WW_Pin1_4	686	691	PF00397	0.646
LIG_14-3-3_CanoR_1	214	220	PF00244	0.506
LIG_14-3-3_CanoR_1	251	255	PF00244	0.406
LIG_14-3-3_CanoR_1	351	358	PF00244	0.455
LIG_14-3-3_CanoR_1	457	461	PF00244	0.352
LIG_14-3-3_CanoR_1	49	55	PF00244	0.529
LIG_14-3-3_CanoR_1	709	718	PF00244	0.508
LIG_BRCT_BRCA1_1	337	341	PF00533	0.318
LIG_BRCT_BRCA1_1	688	692	PF00533	0.631
LIG_Clathr_ClatBox_1	16	20	PF01394	0.719
LIG_eIF4E_1	410	416	PF01652	0.449
LIG_FHA_1	254	260	PF00498	0.327
LIG_FHA_1	280	286	PF00498	0.453
LIG_FHA_1	319	325	PF00498	0.685
LIG_FHA_1	410	416	PF00498	0.449
LIG_FHA_1	444	450	PF00498	0.387
LIG_FHA_1	512	518	PF00498	0.539
LIG_FHA_1	589	595	PF00498	0.635
LIG_FHA_1	634	640	PF00498	0.652
LIG_FHA_1	689	695	PF00498	0.641
LIG_FHA_1	702	708	PF00498	0.569
LIG_FHA_2	388	394	PF00498	0.337
LIG_FHA_2	511	517	PF00498	0.490
LIG_FHA_2	92	98	PF00498	0.710
LIG_Integrin_isoDGR_2	500	502	PF01839	0.475
LIG_Integrin_RGD_1	555	557	PF01839	0.679
LIG_LIR_Apic_2	128	132	PF02991	0.459
LIG_LIR_Apic_2	293	297	PF02991	0.513
LIG_LIR_Apic_2	526	530	PF02991	0.489
LIG_LIR_Gen_1	187	196	PF02991	0.706
LIG_LIR_Gen_1	390	399	PF02991	0.359
LIG_LIR_Gen_1	573	584	PF02991	0.655
LIG_LIR_Gen_1	98	109	PF02991	0.528
LIG_LIR_LC3C_4	363	367	PF02991	0.223
LIG_LIR_LC3C_4	674	679	PF02991	0.528
LIG_LIR_Nem_3	159	163	PF02991	0.382
LIG_LIR_Nem_3	187	193	PF02991	0.697
LIG_LIR_Nem_3	260	265	PF02991	0.431
LIG_LIR_Nem_3	390	394	PF02991	0.402
LIG_LIR_Nem_3	396	401	PF02991	0.345
LIG_LIR_Nem_3	56	62	PF02991	0.682
LIG_LIR_Nem_3	573	579	PF02991	0.633
LIG_LIR_Nem_3	98	104	PF02991	0.521
LIG_LYPXL_L_2	265	274	PF13949	0.307
LIG_LYPXL_S_1	265	269	PF13949	0.286
LIG_LYPXL_yS_3	266	269	PF13949	0.288
LIG_NRBOX	12	18	PF00104	0.594
LIG_PDZ_Class_2	723	728	PF00595	0.590
LIG_Pex14_1	576	580	PF04695	0.736
LIG_Pex14_2	371	375	PF04695	0.449
LIG_SH2_CRK	504	508	PF00017	0.330
LIG_SH2_CRK	59	63	PF00017	0.679
LIG_SH2_NCK_1	441	445	PF00017	0.449
LIG_SH2_PTP2	527	530	PF00017	0.464
LIG_SH2_SRC	441	444	PF00017	0.449
LIG_SH2_SRC	527	530	PF00017	0.690
LIG_SH2_SRC	638	641	PF00017	0.598
LIG_SH2_STAP1	255	259	PF00017	0.446
LIG_SH2_STAT3	161	164	PF00017	0.306
LIG_SH2_STAT3	255	258	PF00017	0.537
LIG_SH2_STAT5	161	164	PF00017	0.515
LIG_SH2_STAT5	255	258	PF00017	0.423
LIG_SH2_STAT5	527	530	PF00017	0.651
LIG_SH2_STAT5	59	62	PF00017	0.571
LIG_SH2_STAT5	638	641	PF00017	0.598
LIG_SH3_2	300	305	PF14604	0.641
LIG_SH3_3	101	107	PF00018	0.563
LIG_SH3_3	140	146	PF00018	0.564
LIG_SH3_3	294	300	PF00018	0.408
LIG_SH3_3	303	309	PF00018	0.543
LIG_SH3_3	364	370	PF00018	0.371
LIG_SH3_3	411	417	PF00018	0.409
LIG_SH3_3	431	437	PF00018	0.339
LIG_SH3_3	696	702	PF00018	0.443
LIG_SH3_4	106	113	PF00018	0.544
LIG_SUMO_SIM_anti_2	363	368	PF11976	0.361
LIG_SUMO_SIM_anti_2	674	680	PF11976	0.476
LIG_SUMO_SIM_par_1	420	425	PF11976	0.344
LIG_TRAF2_1	229	232	PF00917	0.718
LIG_TRAF2_1	240	243	PF00917	0.638
LIG_UBA3_1	415	420	PF00899	0.449
LIG_UBA3_1	534	538	PF00899	0.638
MOD_CK1_1	147	153	PF00069	0.596
MOD_CK1_1	202	208	PF00069	0.615
MOD_CK1_1	253	259	PF00069	0.401
MOD_CK1_1	260	266	PF00069	0.386
MOD_CK1_1	314	320	PF00069	0.586
MOD_CK1_1	328	334	PF00069	0.403
MOD_CK1_1	40	46	PF00069	0.652
MOD_CK1_1	53	59	PF00069	0.543
MOD_CK1_1	566	572	PF00069	0.691
MOD_CK1_1	618	624	PF00069	0.660
MOD_CK1_1	66	72	PF00069	0.528
MOD_CK1_1	685	691	PF00069	0.622
MOD_CK2_1	374	380	PF00069	0.365
MOD_CK2_1	387	393	PF00069	0.250
MOD_CK2_1	91	97	PF00069	0.667
MOD_GlcNHglycan	145	149	PF01048	0.611
MOD_GlcNHglycan	201	204	PF01048	0.706
MOD_GlcNHglycan	205	208	PF01048	0.733
MOD_GlcNHglycan	311	314	PF01048	0.676
MOD_GlcNHglycan	337	340	PF01048	0.226
MOD_GlcNHglycan	345	349	PF01048	0.475
MOD_GlcNHglycan	40	43	PF01048	0.623
MOD_GlcNHglycan	543	546	PF01048	0.716
MOD_GlcNHglycan	547	550	PF01048	0.744
MOD_GlcNHglycan	559	562	PF01048	0.803
MOD_GlcNHglycan	568	571	PF01048	0.728
MOD_GlcNHglycan	581	585	PF01048	0.608
MOD_GlcNHglycan	620	623	PF01048	0.741
MOD_GlcNHglycan	64	68	PF01048	0.584
MOD_GlcNHglycan	671	674	PF01048	0.538
MOD_GlcNHglycan	713	716	PF01048	0.463
MOD_GlcNHglycan	97	100	PF01048	0.567
MOD_GSK3_1	199	206	PF00069	0.580
MOD_GSK3_1	209	216	PF00069	0.657
MOD_GSK3_1	253	260	PF00069	0.493
MOD_GSK3_1	309	316	PF00069	0.595
MOD_GSK3_1	318	325	PF00069	0.621
MOD_GSK3_1	383	390	PF00069	0.342
MOD_GSK3_1	483	490	PF00069	0.348
MOD_GSK3_1	50	57	PF00069	0.516
MOD_GSK3_1	541	548	PF00069	0.693
MOD_GSK3_1	562	569	PF00069	0.684
MOD_GSK3_1	580	587	PF00069	0.582
MOD_GSK3_1	682	689	PF00069	0.642
MOD_GSK3_1	697	704	PF00069	0.315
MOD_GSK3_1	91	98	PF00069	0.717
MOD_NEK2_1	149	154	PF00069	0.566
MOD_NEK2_1	179	184	PF00069	0.503
MOD_NEK2_1	209	214	PF00069	0.739
MOD_NEK2_1	26	31	PF00069	0.438
MOD_NEK2_1	278	283	PF00069	0.454
MOD_NEK2_1	311	316	PF00069	0.607
MOD_NEK2_1	387	392	PF00069	0.355
MOD_NEK2_1	402	407	PF00069	0.312
MOD_NEK2_1	422	427	PF00069	0.441
MOD_NEK2_1	450	455	PF00069	0.449
MOD_NEK2_1	54	59	PF00069	0.556
MOD_NEK2_1	564	569	PF00069	0.541
MOD_NEK2_1	580	585	PF00069	0.629
MOD_NEK2_1	590	595	PF00069	0.728
MOD_NEK2_1	91	96	PF00069	0.644
MOD_NEK2_2	487	492	PF00069	0.449
MOD_PIKK_1	138	144	PF00454	0.404
MOD_PIKK_1	150	156	PF00454	0.578
MOD_PIKK_1	428	434	PF00454	0.328
MOD_PIKK_1	91	97	PF00454	0.696
MOD_PK_1	214	220	PF00069	0.487
MOD_PKA_2	213	219	PF00069	0.620
MOD_PKA_2	250	256	PF00069	0.394
MOD_PKA_2	260	266	PF00069	0.398
MOD_PKA_2	279	285	PF00069	0.291
MOD_PKA_2	456	462	PF00069	0.358
MOD_PKA_2	48	54	PF00069	0.539
MOD_Plk_1	111	117	PF00069	0.675
MOD_Plk_1	186	192	PF00069	0.744
MOD_Plk_1	26	32	PF00069	0.701
MOD_Plk_1	344	350	PF00069	0.298
MOD_Plk_1	402	408	PF00069	0.350
MOD_Plk_1	428	434	PF00069	0.315
MOD_Plk_1	697	703	PF00069	0.430
MOD_Plk_4	250	256	PF00069	0.392
MOD_Plk_4	26	32	PF00069	0.648
MOD_Plk_4	436	442	PF00069	0.343
MOD_ProDKin_1	29	35	PF00069	0.619
MOD_ProDKin_1	686	692	PF00069	0.640
MOD_SUMO_for_1	105	108	PF00179	0.669
MOD_SUMO_for_1	44	47	PF00179	0.463
MOD_SUMO_for_1	599	602	PF00179	0.659
MOD_SUMO_rev_2	533	540	PF00179	0.673
TRG_DiLeu_BaLyEn_6	306	311	PF01217	0.713
TRG_DiLeu_BaLyEn_6	411	416	PF01217	0.449
TRG_ENDOCYTIC_2	266	269	PF00928	0.315
TRG_ENDOCYTIC_2	504	507	PF00928	0.328
TRG_ENDOCYTIC_2	59	62	PF00928	0.649
TRG_ENDOCYTIC_2	78	81	PF00928	0.229
TRG_ER_diArg_1	342	344	PF00400	0.363
TRG_ER_diArg_1	491	493	PF00400	0.344
TRG_ER_diArg_1	552	555	PF00400	0.538
TRG_ER_diArg_1	708	710	PF00400	0.538
TRG_NLS_MonoExtC_3	596	602	PF00514	0.654
TRG_NLS_MonoExtN_4	595	601	PF00514	0.725

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IAT5	Leptomonas seymouri	62%	100%
A0A0S4JBE4	Bodo saltans	28%	100%
A0A1X0NW10	Trypanosomatidae	42%	100%
A0A3Q8IL35	Leishmania donovani	76%	100%
A0A3R7NGI8	Trypanosoma rangeli	43%	100%
A4I7Y7	Leishmania infantum	76%	100%
D0AA49	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	100%
E9B2U8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	77%	100%
Q4Q5A8	Leishmania major	76%	99%
V5DFV4	Trypanosoma cruzi	42%	100%