mRNA guanylyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RNA capping enzyme, cytoplasmic

Species:

Leishmania braziliensis

UniProt:

A4HKC4_LEIBR

TriTrypDb:

LbrM.32.1520 , LBRM2903_320021300

Length:

688

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HKC4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HKC4

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	9
GO:0006370	7-methylguanosine mRNA capping	8	9
GO:0006396	RNA processing	6	9
GO:0006397	mRNA processing	7	9
GO:0006725	cellular aromatic compound metabolic process	3	9
GO:0006807	nitrogen compound metabolic process	2	9
GO:0008152	metabolic process	1	9
GO:0009452	7-methylguanosine RNA capping	8	9
GO:0009987	cellular process	1	9
GO:0016070	RNA metabolic process	5	9
GO:0016071	mRNA metabolic process	6	9
GO:0034641	cellular nitrogen compound metabolic process	3	9
GO:0036260	RNA capping	7	9
GO:0043170	macromolecule metabolic process	3	9
GO:0044237	cellular metabolic process	2	9
GO:0044238	primary metabolic process	2	9
GO:0046483	heterocycle metabolic process	3	9
GO:0071704	organic substance metabolic process	2	9
GO:0090304	nucleic acid metabolic process	4	9
GO:1901360	organic cyclic compound metabolic process	3	9

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	9
GO:0003824	catalytic activity	1	9
GO:0004484	mRNA guanylyltransferase activity	5	9
GO:0005488	binding	1	9
GO:0005524	ATP binding	5	9
GO:0005525	GTP binding	5	5
GO:0008192	RNA guanylyltransferase activity	4	9
GO:0016740	transferase activity	2	9
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	9
GO:0016779	nucleotidyltransferase activity	4	9
GO:0017076	purine nucleotide binding	4	9
GO:0019001	guanyl nucleotide binding	5	5
GO:0030554	adenyl nucleotide binding	5	9
GO:0032553	ribonucleotide binding	3	9
GO:0032555	purine ribonucleotide binding	4	9
GO:0032559	adenyl ribonucleotide binding	5	9
GO:0032561	guanyl ribonucleotide binding	5	5
GO:0035639	purine ribonucleoside triphosphate binding	4	9
GO:0036094	small molecule binding	2	9
GO:0043167	ion binding	2	9
GO:0043168	anion binding	3	9
GO:0070568	guanylyltransferase activity	5	9
GO:0097159	organic cyclic compound binding	2	9
GO:0097367	carbohydrate derivative binding	2	9
GO:0140098	catalytic activity, acting on RNA	3	9
GO:0140640	catalytic activity, acting on a nucleic acid	2	9
GO:1901265	nucleoside phosphate binding	3	9
GO:1901363	heterocyclic compound binding	2	9
GO:0019002	GMP binding	4	1
GO:0003909	DNA ligase activity	4	1
GO:0003910	DNA ligase (ATP) activity	5	1
GO:0016874	ligase activity	2	1
GO:0016886	ligase activity, forming phosphoric ester bonds	3	1
GO:0140097	catalytic activity, acting on DNA	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	300	304	PF00656	0.512
CLV_NRD_NRD_1	127	129	PF00675	0.745
CLV_NRD_NRD_1	244	246	PF00675	0.585
CLV_NRD_NRD_1	292	294	PF00675	0.560
CLV_PCSK_KEX2_1	244	246	PF00082	0.473
CLV_PCSK_KEX2_1	292	294	PF00082	0.560
CLV_PCSK_KEX2_1	308	310	PF00082	0.610
CLV_PCSK_KEX2_1	378	380	PF00082	0.493
CLV_PCSK_KEX2_1	479	481	PF00082	0.416
CLV_PCSK_PC1ET2_1	308	310	PF00082	0.585
CLV_PCSK_PC1ET2_1	378	380	PF00082	0.533
CLV_PCSK_PC1ET2_1	479	481	PF00082	0.462
CLV_PCSK_PC7_1	374	380	PF00082	0.551
CLV_PCSK_SKI1_1	184	188	PF00082	0.358
CLV_PCSK_SKI1_1	476	480	PF00082	0.465
CLV_PCSK_SKI1_1	552	556	PF00082	0.462
CLV_PCSK_SKI1_1	664	668	PF00082	0.509
CLV_Separin_Metazoa	488	492	PF03568	0.462
DEG_APCC_DBOX_1	159	167	PF00400	0.571
DEG_APCC_KENBOX_2	186	190	PF00400	0.336
DEG_SCF_FBW7_1	67	72	PF00400	0.583
DOC_CDC14_PxL_1	518	526	PF14671	0.462
DOC_CKS1_1	317	322	PF01111	0.543
DOC_CKS1_1	90	95	PF01111	0.731
DOC_CYCLIN_RxL_1	552	566	PF00134	0.512
DOC_CYCLIN_RxL_1	673	681	PF00134	0.552
DOC_CYCLIN_yCln2_LP_2	227	233	PF00134	0.371
DOC_CYCLIN_yCln2_LP_2	405	411	PF00134	0.462
DOC_CYCLIN_yCln2_LP_2	420	426	PF00134	0.223
DOC_MAPK_DCC_7	225	233	PF00069	0.344
DOC_MAPK_gen_1	195	204	PF00069	0.457
DOC_MAPK_gen_1	276	283	PF00069	0.522
DOC_MAPK_gen_1	308	317	PF00069	0.596
DOC_MAPK_gen_1	378	385	PF00069	0.462
DOC_MAPK_MEF2A_6	225	233	PF00069	0.312
DOC_MAPK_MEF2A_6	308	317	PF00069	0.345
DOC_MAPK_MEF2A_6	378	387	PF00069	0.462
DOC_MAPK_MEF2A_6	613	622	PF00069	0.462
DOC_MAPK_RevD_3	278	293	PF00069	0.552
DOC_PP1_RVXF_1	674	681	PF00149	0.553
DOC_PP2B_LxvP_1	227	230	PF13499	0.371
DOC_PP4_FxxP_1	680	683	PF00568	0.556
DOC_SPAK_OSR1_1	393	397	PF12202	0.301
DOC_USP7_MATH_1	132	136	PF00917	0.575
DOC_USP7_MATH_1	358	362	PF00917	0.490
DOC_USP7_MATH_1	54	58	PF00917	0.708
DOC_USP7_MATH_1	9	13	PF00917	0.738
DOC_USP7_UBL2_3	552	556	PF12436	0.346
DOC_WW_Pin1_4	316	321	PF00397	0.536
DOC_WW_Pin1_4	42	47	PF00397	0.740
DOC_WW_Pin1_4	478	483	PF00397	0.462
DOC_WW_Pin1_4	65	70	PF00397	0.798
DOC_WW_Pin1_4	89	94	PF00397	0.698
LIG_14-3-3_CanoR_1	379	384	PF00244	0.337
LIG_14-3-3_CanoR_1	613	617	PF00244	0.462
LIG_14-3-3_CanoR_1	650	660	PF00244	0.423
LIG_Actin_WH2_2	228	246	PF00022	0.549
LIG_Actin_WH2_2	598	615	PF00022	0.462
LIG_Actin_WH2_2	92	110	PF00022	0.537
LIG_APCC_ABBA_1	599	604	PF00400	0.462
LIG_APCC_Cbox_2	205	211	PF00515	0.467
LIG_BIR_II_1	1	5	PF00653	0.730
LIG_BIR_III_3	1	5	PF00653	0.752
LIG_EH1_1	532	540	PF00400	0.462
LIG_eIF4E_1	324	330	PF01652	0.528
LIG_eIF4E_1	415	421	PF01652	0.346
LIG_FHA_1	30	36	PF00498	0.547
LIG_FHA_1	384	390	PF00498	0.378
LIG_FHA_1	635	641	PF00498	0.449
LIG_FHA_1	652	658	PF00498	0.321
LIG_FHA_1	74	80	PF00498	0.550
LIG_FHA_1	90	96	PF00498	0.512
LIG_FHA_2	131	137	PF00498	0.565
LIG_FHA_2	150	156	PF00498	0.479
LIG_FHA_2	578	584	PF00498	0.564
LIG_FHA_2	585	591	PF00498	0.547
LIG_FHA_2	665	671	PF00498	0.498
LIG_HCF-1_HBM_1	272	275	PF13415	0.313
LIG_Integrin_RGD_1	249	251	PF01839	0.591
LIG_LIR_Gen_1	159	166	PF02991	0.508
LIG_LIR_Gen_1	296	307	PF02991	0.516
LIG_LIR_Gen_1	325	333	PF02991	0.446
LIG_LIR_Gen_1	334	343	PF02991	0.345
LIG_LIR_Gen_1	381	389	PF02991	0.440
LIG_LIR_Gen_1	392	402	PF02991	0.144
LIG_LIR_Gen_1	416	424	PF02991	0.403
LIG_LIR_Gen_1	469	478	PF02991	0.326
LIG_LIR_Gen_1	485	494	PF02991	0.326
LIG_LIR_Gen_1	580	588	PF02991	0.451
LIG_LIR_Gen_1	667	672	PF02991	0.495
LIG_LIR_Nem_3	159	164	PF02991	0.491
LIG_LIR_Nem_3	198	202	PF02991	0.449
LIG_LIR_Nem_3	257	262	PF02991	0.600
LIG_LIR_Nem_3	272	278	PF02991	0.311
LIG_LIR_Nem_3	296	302	PF02991	0.409
LIG_LIR_Nem_3	325	330	PF02991	0.426
LIG_LIR_Nem_3	331	336	PF02991	0.363
LIG_LIR_Nem_3	381	387	PF02991	0.407
LIG_LIR_Nem_3	392	397	PF02991	0.144
LIG_LIR_Nem_3	400	405	PF02991	0.431
LIG_LIR_Nem_3	416	420	PF02991	0.355
LIG_LIR_Nem_3	469	473	PF02991	0.326
LIG_LIR_Nem_3	481	487	PF02991	0.326
LIG_LIR_Nem_3	492	496	PF02991	0.301
LIG_LIR_Nem_3	580	585	PF02991	0.514
LIG_LIR_Nem_3	667	671	PF02991	0.501
LIG_MYND_1	522	526	PF01753	0.462
LIG_PCNA_APIM_2	550	556	PF02747	0.462
LIG_PCNA_TLS_4	547	554	PF02747	0.462
LIG_PDZ_Class_1	683	688	PF00595	0.433
LIG_Pex14_2	407	411	PF04695	0.326
LIG_Pex14_2	417	421	PF04695	0.326
LIG_Pex14_2	553	557	PF04695	0.346
LIG_Rb_LxCxE_1	520	536	PF01857	0.303
LIG_REV1ctd_RIR_1	481	490	PF16727	0.440
LIG_SH2_CRK	161	165	PF00017	0.488
LIG_SH2_CRK	199	203	PF00017	0.403
LIG_SH2_CRK	275	279	PF00017	0.411
LIG_SH2_NCK_1	299	303	PF00017	0.458
LIG_SH2_PTP2	384	387	PF00017	0.462
LIG_SH2_PTP2	515	518	PF00017	0.346
LIG_SH2_SRC	365	368	PF00017	0.530
LIG_SH2_SRC	515	518	PF00017	0.326
LIG_SH2_STAP1	16	20	PF00017	0.753
LIG_SH2_STAP1	415	419	PF00017	0.462
LIG_SH2_STAT3	216	219	PF00017	0.477
LIG_SH2_STAT3	261	264	PF00017	0.588
LIG_SH2_STAT5	217	220	PF00017	0.366
LIG_SH2_STAT5	261	264	PF00017	0.602
LIG_SH2_STAT5	365	368	PF00017	0.419
LIG_SH2_STAT5	384	387	PF00017	0.402
LIG_SH2_STAT5	470	473	PF00017	0.343
LIG_SH2_STAT5	515	518	PF00017	0.326
LIG_SH3_3	45	51	PF00018	0.766
LIG_SH3_3	455	461	PF00018	0.239
LIG_SH3_3	590	596	PF00018	0.536
LIG_SH3_3	643	649	PF00018	0.462
LIG_SH3_3	87	93	PF00018	0.622
LIG_SUMO_SIM_anti_2	135	142	PF11976	0.409
LIG_SUMO_SIM_par_1	615	621	PF11976	0.441
LIG_TRAF2_1	667	670	PF00917	0.441
LIG_TYR_ITIM	666	671	PF00017	0.496
LIG_UBA3_1	301	308	PF00899	0.514
LIG_UBA3_1	420	425	PF00899	0.239
MOD_CDK_SPK_2	89	94	PF00069	0.531
MOD_CDK_SPxxK_3	42	49	PF00069	0.570
MOD_CK1_1	148	154	PF00069	0.513
MOD_CK1_1	351	357	PF00069	0.596
MOD_CK2_1	149	155	PF00069	0.527
MOD_CK2_1	438	444	PF00069	0.462
MOD_CK2_1	451	457	PF00069	0.468
MOD_CK2_1	584	590	PF00069	0.547
MOD_CK2_1	652	658	PF00069	0.426
MOD_CK2_1	664	670	PF00069	0.346
MOD_Cter_Amidation	126	129	PF01082	0.736
MOD_GlcNHglycan	12	15	PF01048	0.744
MOD_GlcNHglycan	147	150	PF01048	0.369
MOD_GlcNHglycan	16	19	PF01048	0.689
MOD_GlcNHglycan	167	170	PF01048	0.144
MOD_GlcNHglycan	350	353	PF01048	0.549
MOD_GlcNHglycan	355	358	PF01048	0.417
MOD_GlcNHglycan	438	443	PF01048	0.263
MOD_GlcNHglycan	500	503	PF01048	0.444
MOD_GlcNHglycan	52	55	PF01048	0.751
MOD_GlcNHglycan	634	637	PF01048	0.239
MOD_GlcNHglycan	69	72	PF01048	0.624
MOD_GlcNHglycan	84	87	PF01048	0.561
MOD_GlcNHglycan	96	99	PF01048	0.701
MOD_GSK3_1	10	17	PF00069	0.747
MOD_GSK3_1	145	152	PF00069	0.529
MOD_GSK3_1	347	354	PF00069	0.517
MOD_GSK3_1	379	386	PF00069	0.364
MOD_GSK3_1	50	57	PF00069	0.764
MOD_GSK3_1	59	66	PF00069	0.787
MOD_GSK3_1	652	659	PF00069	0.482
MOD_GSK3_1	69	76	PF00069	0.508
MOD_GSK3_1	94	101	PF00069	0.712
MOD_N-GLC_1	195	200	PF02516	0.358
MOD_N-GLC_1	309	314	PF02516	0.511
MOD_N-GLC_1	348	353	PF02516	0.566
MOD_N-GLC_1	651	656	PF02516	0.398
MOD_N-GLC_1	683	688	PF02516	0.656
MOD_NEK2_1	164	169	PF00069	0.479
MOD_NEK2_1	383	388	PF00069	0.398
MOD_NEK2_1	409	414	PF00069	0.490
MOD_NEK2_1	451	456	PF00069	0.462
MOD_NEK2_1	612	617	PF00069	0.462
MOD_NEK2_1	678	683	PF00069	0.558
MOD_PIKK_1	149	155	PF00454	0.474
MOD_PIKK_1	634	640	PF00454	0.490
MOD_PK_1	297	303	PF00069	0.522
MOD_PK_1	309	315	PF00069	0.274
MOD_PKA_1	378	384	PF00069	0.462
MOD_PKA_2	145	151	PF00069	0.555
MOD_PKA_2	378	384	PF00069	0.462
MOD_PKA_2	612	618	PF00069	0.475
MOD_PKA_2	678	684	PF00069	0.565
MOD_Plk_1	195	201	PF00069	0.571
MOD_Plk_1	309	315	PF00069	0.505
MOD_Plk_1	366	372	PF00069	0.430
MOD_Plk_1	651	657	PF00069	0.461
MOD_Plk_2-3	577	583	PF00069	0.574
MOD_Plk_4	297	303	PF00069	0.509
MOD_Plk_4	379	385	PF00069	0.462
MOD_Plk_4	541	547	PF00069	0.462
MOD_Plk_4	577	583	PF00069	0.560
MOD_Plk_4	612	618	PF00069	0.384
MOD_Plk_4	652	658	PF00069	0.363
MOD_ProDKin_1	316	322	PF00069	0.545
MOD_ProDKin_1	42	48	PF00069	0.739
MOD_ProDKin_1	478	484	PF00069	0.462
MOD_ProDKin_1	65	71	PF00069	0.798
MOD_ProDKin_1	89	95	PF00069	0.701
MOD_SUMO_rev_2	168	178	PF00179	0.507
MOD_SUMO_rev_2	248	254	PF00179	0.593
MOD_SUMO_rev_2	370	380	PF00179	0.547
TRG_DiLeu_BaEn_1	159	164	PF01217	0.571
TRG_DiLeu_BaEn_1	325	330	PF01217	0.430
TRG_DiLeu_BaEn_1	485	490	PF01217	0.462
TRG_DiLeu_BaEn_1	492	497	PF01217	0.350
TRG_DiLeu_BaLyEn_6	134	139	PF01217	0.549
TRG_DiLeu_BaLyEn_6	460	465	PF01217	0.462
TRG_ENDOCYTIC_2	161	164	PF00928	0.488
TRG_ENDOCYTIC_2	199	202	PF00928	0.408
TRG_ENDOCYTIC_2	275	278	PF00928	0.417
TRG_ENDOCYTIC_2	299	302	PF00928	0.545
TRG_ENDOCYTIC_2	324	327	PF00928	0.470
TRG_ENDOCYTIC_2	333	336	PF00928	0.349
TRG_ENDOCYTIC_2	384	387	PF00928	0.435
TRG_ENDOCYTIC_2	470	473	PF00928	0.400
TRG_ENDOCYTIC_2	487	490	PF00928	0.400
TRG_ENDOCYTIC_2	515	518	PF00928	0.317
TRG_ENDOCYTIC_2	668	671	PF00928	0.468
TRG_ER_diArg_1	243	245	PF00400	0.600
TRG_ER_diArg_1	292	294	PF00400	0.560
TRG_ER_diArg_1	643	646	PF00400	0.462
TRG_NES_CRM1_1	226	241	PF08389	0.531
TRG_NES_CRM1_1	611	624	PF08389	0.398
TRG_NLS_Bipartite_1	292	312	PF00514	0.550
TRG_NLS_MonoExtN_4	307	312	PF00514	0.587

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4IM27	Bodo saltans	34%	96%
A0A1X0NUI5	Trypanosomatidae	40%	100%
A0A3S7X5E2	Leishmania donovani	85%	99%
A0A422P1D1	Trypanosoma rangeli	41%	100%
D0AA12	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E9B2R1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	100%
Q4Q5E6	Leishmania major	85%	100%
V5DHN7	Trypanosoma cruzi	42%	100%