Putative ubiquitin-conjugating enzyme protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme protein, putative

Species:

Leishmania braziliensis

UniProt:

A4HK77_LEIBR

TriTrypDb:

LbrM.32.1050 , LBRM2903_320015900

Length:

271

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HK77

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HK77

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	1
GO:0006508	proteolysis	4	1
GO:0006511	ubiquitin-dependent protein catabolic process	7	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009987	cellular process	1	1
GO:0016567	protein ubiquitination	7	1
GO:0019538	protein metabolic process	3	1
GO:0019941	modification-dependent protein catabolic process	6	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043632	modification-dependent macromolecule catabolic process	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0051603	proteolysis involved in protein catabolic process	5	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901575	organic substance catabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016740	transferase activity	2	11
GO:0016874	ligase activity	2	7
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12
GO:0004842	ubiquitin-protein transferase activity	4	2
GO:0019787	ubiquitin-like protein transferase activity	3	2
GO:0061631	ubiquitin conjugating enzyme activity	5	2
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	2
GO:0140096	catalytic activity, acting on a protein	2	2
GO:0016746	acyltransferase activity	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	25	29	PF00656	0.392
CLV_PCSK_FUR_1	146	150	PF00082	0.325
CLV_PCSK_KEX2_1	148	150	PF00082	0.430
CLV_PCSK_PC1ET2_1	148	150	PF00082	0.192
CLV_PCSK_SKI1_1	115	119	PF00082	0.294
CLV_PCSK_SKI1_1	12	16	PF00082	0.315
CLV_PCSK_SKI1_1	162	166	PF00082	0.455
DEG_APCC_DBOX_1	114	122	PF00400	0.284
DEG_APCC_DBOX_1	259	267	PF00400	0.481
DEG_SCF_FBW7_1	106	113	PF00400	0.249
DOC_MAPK_gen_1	229	235	PF00069	0.310
DOC_PP2B_LxvP_1	90	93	PF13499	0.249
DOC_PP4_FxxP_1	171	174	PF00568	0.416
DOC_PP4_FxxP_1	38	41	PF00568	0.249
DOC_PP4_FxxP_1	74	77	PF00568	0.309
DOC_PP4_MxPP_1	63	66	PF00568	0.249
DOC_USP7_MATH_1	128	132	PF00917	0.415
DOC_USP7_MATH_1	160	164	PF00917	0.411
DOC_USP7_MATH_1	213	217	PF00917	0.684
DOC_USP7_MATH_1	221	225	PF00917	0.585
DOC_WW_Pin1_4	106	111	PF00397	0.249
DOC_WW_Pin1_4	41	46	PF00397	0.249
LIG_14-3-3_CanoR_1	69	75	PF00244	0.343
LIG_AP_GAE_1	202	208	PF02883	0.670
LIG_BIR_II_1	1	5	PF00653	0.388
LIG_BRCT_BRCA1_1	53	57	PF00533	0.392
LIG_BRCT_BRCA1_2	53	59	PF00533	0.392
LIG_CSL_BTD_1	74	77	PF09270	0.249
LIG_EVH1_2	18	22	PF00568	0.264
LIG_FHA_1	31	37	PF00498	0.309
LIG_FHA_1	65	71	PF00498	0.392
LIG_FHA_2	37	43	PF00498	0.392
LIG_LIR_Apic_2	123	128	PF02991	0.392
LIG_LIR_Apic_2	169	174	PF02991	0.567
LIG_LIR_Apic_2	72	77	PF02991	0.309
LIG_LIR_Gen_1	188	197	PF02991	0.657
LIG_LIR_Gen_1	202	213	PF02991	0.541
LIG_LIR_Gen_1	30	38	PF02991	0.410
LIG_LIR_Nem_3	10	14	PF02991	0.392
LIG_LIR_Nem_3	202	208	PF02991	0.668
LIG_LIR_Nem_3	30	35	PF02991	0.410
LIG_LIR_Nem_3	67	71	PF02991	0.322
LIG_MAD2	69	77	PF02301	0.170
LIG_Pex14_1	34	38	PF04695	0.249
LIG_Pex14_2	57	61	PF04695	0.249
LIG_SH2_SRC	80	83	PF00017	0.309
LIG_SH2_STAP1	231	235	PF00017	0.451
LIG_SH3_1	59	65	PF00018	0.264
LIG_SH3_3	59	65	PF00018	0.354
LIG_SUMO_SIM_par_1	116	123	PF11976	0.250
LIG_UBA3_1	252	258	PF00899	0.374
LIG_WRC_WIRS_1	71	76	PF05994	0.309
MOD_CK1_1	100	106	PF00069	0.343
MOD_CK1_1	120	126	PF00069	0.254
MOD_CK1_1	30	36	PF00069	0.418
MOD_CK2_1	155	161	PF00069	0.421
MOD_CK2_1	36	42	PF00069	0.343
MOD_CMANNOS	34	37	PF00535	0.249
MOD_GlcNHglycan	1	4	PF01048	0.390
MOD_GlcNHglycan	185	188	PF01048	0.548
MOD_GlcNHglycan	206	211	PF01048	0.693
MOD_GlcNHglycan	215	218	PF01048	0.627
MOD_GSK3_1	106	113	PF00069	0.266
MOD_N-GLC_1	12	17	PF02516	0.392
MOD_NEK2_1	208	213	PF00069	0.448
MOD_NEK2_2	110	115	PF00069	0.284
MOD_Plk_1	100	106	PF00069	0.264
MOD_Plk_1	160	166	PF00069	0.521
MOD_Plk_1	27	33	PF00069	0.392
MOD_Plk_2-3	245	251	PF00069	0.503
MOD_Plk_2-3	97	103	PF00069	0.309
MOD_Plk_4	117	123	PF00069	0.255
MOD_Plk_4	262	268	PF00069	0.354
MOD_Plk_4	27	33	PF00069	0.415
MOD_Plk_4	70	76	PF00069	0.249
MOD_ProDKin_1	106	112	PF00069	0.249
MOD_ProDKin_1	41	47	PF00069	0.249
MOD_SUMO_rev_2	172	180	PF00179	0.602
TRG_NES_CRM1_1	112	127	PF08389	0.264
TRG_NES_CRM1_1	234	245	PF08389	0.381
TRG_Pf-PMV_PEXEL_1	12	17	PF00026	0.261
TRG_Pf-PMV_PEXEL_1	157	161	PF00026	0.396
TRG_Pf-PMV_PEXEL_1	162	166	PF00026	0.387

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDU3	Leptomonas seymouri	81%	100%
A0A0S4KIJ6	Bodo saltans	54%	100%
A0A1X0NV24	Trypanosomatidae	57%	100%
A0A3Q8IKW3	Leishmania donovani	95%	100%
A0A3R7LRA8	Trypanosoma rangeli	57%	84%
A4I7R2	Leishmania infantum	95%	100%
D0A9W7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	54%	100%
E9ALA2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E9B2J3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
E9B2M0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
P0C8G3	African swine fever virus (isolate Pig/Kenya/KEN-50/1950)	39%	100%
P14682	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	40%	92%
Q29503	Oryctolagus cuniculus	42%	100%
Q3B7D1	Rattus norvegicus	27%	76%
Q3UE37	Mus musculus	27%	76%
Q4Q5I6	Leishmania major	93%	100%
Q4QIK2	Leishmania major	33%	100%
Q66KB0	Xenopus tropicalis	25%	87%
Q6CMG6	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	25%	100%
Q6PCF7	Xenopus laevis	25%	87%
Q6ZWZ2	Mus musculus	42%	100%
Q712K3	Homo sapiens	42%	100%
Q9H832	Homo sapiens	27%	77%
Q9NPD8	Homo sapiens	29%	100%
V5BGK9	Trypanosoma cruzi	60%	100%