Putative ubiquitin carrier protein 4

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme E2, putative

Species:

Leishmania braziliensis

UniProt:

A4HK50_LEIBR

TriTrypDb:

LbrM.32.0780 , LBRM2903_320013000

Length:

233

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 18
NetGPI	no	yes: 0, no: 18

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

A4HK50

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HK50

PDB structure(s): 1yf9_A , 1yf9_B , 1yf9_C

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	3
GO:0006508	proteolysis	4	3
GO:0006511	ubiquitin-dependent protein catabolic process	7	3
GO:0006807	nitrogen compound metabolic process	2	3
GO:0008152	metabolic process	1	3
GO:0009056	catabolic process	2	3
GO:0009057	macromolecule catabolic process	4	3
GO:0009987	cellular process	1	3
GO:0016567	protein ubiquitination	7	3
GO:0019538	protein metabolic process	3	3
GO:0019941	modification-dependent protein catabolic process	6	3
GO:0032446	protein modification by small protein conjugation	6	3
GO:0036211	protein modification process	4	3
GO:0043170	macromolecule metabolic process	3	3
GO:0043412	macromolecule modification	4	3
GO:0043632	modification-dependent macromolecule catabolic process	5	3
GO:0044237	cellular metabolic process	2	3
GO:0044238	primary metabolic process	2	3
GO:0044248	cellular catabolic process	3	3
GO:0044260	obsolete cellular macromolecule metabolic process	3	3
GO:0044265	obsolete cellular macromolecule catabolic process	4	3
GO:0051603	proteolysis involved in protein catabolic process	5	3
GO:0070647	protein modification by small protein conjugation or removal	5	3
GO:0071704	organic substance metabolic process	2	3
GO:1901564	organonitrogen compound metabolic process	3	3
GO:1901575	organic substance catabolic process	3	3
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006259	DNA metabolic process	4	1
GO:0006281	DNA repair	5	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006950	response to stress	2	1
GO:0006974	DNA damage response	4	1
GO:0010498	proteasomal protein catabolic process	5	2
GO:0016570	histone modification	5	1
GO:0016574	histone ubiquitination	6	1
GO:0030163	protein catabolic process	4	2
GO:0033554	cellular response to stress	3	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	6	2
GO:0046483	heterocycle metabolic process	3	1
GO:0050896	response to stimulus	1	1
GO:0051716	cellular response to stimulus	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901565	organonitrogen compound catabolic process	4	2
GO:0007346	regulation of mitotic cell cycle	5	1
GO:0010564	regulation of cell cycle process	5	1
GO:0010965	regulation of mitotic sister chromatid separation	6	1
GO:0030071	regulation of mitotic metaphase/anaphase transition	7	1
GO:0031145	anaphase-promoting complex-dependent catabolic process	7	1
GO:0033043	regulation of organelle organization	5	1
GO:0033044	regulation of chromosome organization	6	1
GO:0033045	regulation of sister chromatid segregation	5	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051128	regulation of cellular component organization	4	1
GO:0051726	regulation of cell cycle	4	1
GO:0051983	regulation of chromosome segregation	4	1
GO:0065007	biological regulation	1	1
GO:1901987	regulation of cell cycle phase transition	6	1
GO:1901990	regulation of mitotic cell cycle phase transition	6	1
GO:1902099	regulation of metaphase/anaphase transition of cell cycle	6	1
GO:1905818	regulation of chromosome separation	5	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	15
GO:0003824	catalytic activity	1	15
GO:0005488	binding	1	15
GO:0005524	ATP binding	5	15
GO:0016740	transferase activity	2	14
GO:0016874	ligase activity	2	7
GO:0017076	purine nucleotide binding	4	15
GO:0030554	adenyl nucleotide binding	5	15
GO:0032553	ribonucleotide binding	3	15
GO:0032555	purine ribonucleotide binding	4	15
GO:0032559	adenyl ribonucleotide binding	5	15
GO:0035639	purine ribonucleoside triphosphate binding	4	15
GO:0036094	small molecule binding	2	15
GO:0043167	ion binding	2	15
GO:0043168	anion binding	3	15
GO:0097159	organic cyclic compound binding	2	15
GO:0097367	carbohydrate derivative binding	2	15
GO:1901265	nucleoside phosphate binding	3	15
GO:1901363	heterocyclic compound binding	2	15
GO:0004842	ubiquitin-protein transferase activity	4	4
GO:0016746	acyltransferase activity	3	1
GO:0019787	ubiquitin-like protein transferase activity	3	4
GO:0061631	ubiquitin conjugating enzyme activity	5	4
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	4
GO:0140096	catalytic activity, acting on a protein	2	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	12	14	PF00675	0.404
CLV_NRD_NRD_1	24	26	PF00675	0.254
CLV_PCSK_KEX2_1	11	13	PF00082	0.394
CLV_PCSK_PC7_1	8	14	PF00082	0.500
CLV_PCSK_SKI1_1	194	198	PF00082	0.600
DOC_MAPK_gen_1	11	20	PF00069	0.410
DOC_MAPK_MEF2A_6	84	92	PF00069	0.388
DOC_PP1_RVXF_1	134	140	PF00149	0.267
DOC_PP4_FxxP_1	40	43	PF00568	0.254
DOC_PP4_FxxP_1	65	68	PF00568	0.330
DOC_USP7_MATH_1	118	122	PF00917	0.276
DOC_USP7_MATH_1	156	160	PF00917	0.373
DOC_USP7_MATH_1	190	194	PF00917	0.666
DOC_WW_Pin1_4	149	154	PF00397	0.382
DOC_WW_Pin1_4	199	204	PF00397	0.642
DOC_WW_Pin1_4	43	48	PF00397	0.347
DOC_WW_Pin1_4	64	69	PF00397	0.329
DOC_WW_Pin1_4	95	100	PF00397	0.254
LIG_14-3-3_CanoR_1	19	29	PF00244	0.267
LIG_BIR_II_1	1	5	PF00653	0.548
LIG_BRCT_BRCA1_1	68	72	PF00533	0.254
LIG_CSL_BTD_1	96	99	PF09270	0.254
LIG_deltaCOP1_diTrp_1	35	40	PF00928	0.279
LIG_FHA_1	22	28	PF00498	0.253
LIG_FHA_1	50	56	PF00498	0.278
LIG_FHA_2	168	174	PF00498	0.740
LIG_FHA_2	180	186	PF00498	0.572
LIG_FHA_2	200	206	PF00498	0.632
LIG_FHA_2	30	36	PF00498	0.388
LIG_FHA_2	44	50	PF00498	0.310
LIG_LIR_Apic_2	39	43	PF02991	0.254
LIG_LIR_Apic_2	62	68	PF02991	0.330
LIG_LIR_Gen_1	104	115	PF02991	0.403
LIG_LIR_Gen_1	35	43	PF02991	0.283
LIG_LIR_Nem_3	104	110	PF02991	0.435
LIG_LIR_Nem_3	14	20	PF02991	0.338
LIG_LIR_Nem_3	35	40	PF02991	0.306
LIG_PDZ_Class_2	228	233	PF00595	0.519
LIG_Pex14_1	97	101	PF04695	0.310
LIG_Pex14_2	36	40	PF04695	0.254
LIG_SH2_CRK	17	21	PF00017	0.349
LIG_SH2_SRC	48	51	PF00017	0.276
LIG_SH3_1	117	123	PF00018	0.310
LIG_SH3_3	106	112	PF00018	0.276
LIG_SH3_3	117	123	PF00018	0.276
LIG_SH3_3	54	60	PF00018	0.330
MOD_CDK_SPxK_1	149	155	PF00069	0.385
MOD_CK1_1	199	205	PF00069	0.634
MOD_CK2_1	156	162	PF00069	0.453
MOD_CK2_1	167	173	PF00069	0.516
MOD_CK2_1	199	205	PF00069	0.569
MOD_CK2_1	29	35	PF00069	0.288
MOD_CK2_1	43	49	PF00069	0.424
MOD_GlcNHglycan	1	4	PF01048	0.714
MOD_GlcNHglycan	82	87	PF01048	0.271
MOD_GSK3_1	145	152	PF00069	0.439
MOD_GSK3_1	190	197	PF00069	0.586
MOD_LATS_1	192	198	PF00433	0.566
MOD_N-GLC_1	21	26	PF02516	0.482
MOD_N-GLC_1	93	98	PF02516	0.249
MOD_NEK2_1	1	6	PF00069	0.458
MOD_NEK2_1	196	201	PF00069	0.675
MOD_NEK2_1	20	25	PF00069	0.398
MOD_PK_1	66	72	PF00069	0.254
MOD_PK_1	84	90	PF00069	0.369
MOD_Plk_1	197	203	PF00069	0.625
MOD_Plk_1	93	99	PF00069	0.249
MOD_Plk_4	49	55	PF00069	0.278
MOD_ProDKin_1	149	155	PF00069	0.385
MOD_ProDKin_1	199	205	PF00069	0.642
MOD_ProDKin_1	43	49	PF00069	0.347
MOD_ProDKin_1	64	70	PF00069	0.329
MOD_ProDKin_1	95	101	PF00069	0.254
MOD_SUMO_rev_2	35	43	PF00179	0.267
TRG_ENDOCYTIC_2	17	20	PF00928	0.413
TRG_ER_diArg_1	11	13	PF00400	0.509

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HXA7	Leptomonas seymouri	30%	87%
A0A0N1I1I8	Leptomonas seymouri	73%	100%
A0A0S4IJE0	Bodo saltans	25%	100%
A0A0S4KIJ6	Bodo saltans	26%	94%
A0A1X0NW44	Trypanosomatidae	57%	100%
A0A3Q8IFB8	Leishmania donovani	93%	100%
A0A3S7WX16	Leishmania donovani	30%	90%
A0A3S7WYK3	Leishmania donovani	34%	100%
A0A422NJI9	Trypanosoma rangeli	57%	100%
A4HCA6	Leishmania braziliensis	30%	90%
A4HDS4	Leishmania braziliensis	33%	100%
A4HZT8	Leishmania infantum	30%	90%
A4I122	Leishmania infantum	34%	100%
A4I7L2	Leishmania infantum	93%	100%
C3Z724	Branchiostoma floridae	30%	100%
D0A9T7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	66%	100%
D0A9W7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	94%
E9AVP3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	90%
E9AX59	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
E9B2J3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
P49428	Komagataella pastoris	25%	100%
Q32LD2	Bos taurus	28%	100%
Q4Q5L3	Leishmania major	91%	100%
Q4QAB7	Leishmania major	33%	100%
Q4QBT6	Leishmania major	30%	90%